Patents Examined by Celine Qian
  • Patent number: 10113149
    Abstract: The invention is directed to generation of hematopoietic multi-lineage progenitors (HMLPs) from endothelial cells (ECs) by effecting forced expression of certain transcription factors in the ECs and culturing the ECs in serum free media in the presence of endothelial feeder cells. The HMLPs generated in accordance with this invention can produce erythroid, lymphoid, myeloid, and megakaryocyte cells. These generated HMLPs can be used in therapeutic treatment of disorders including hematopoietic conditions.
    Type: Grant
    Filed: January 15, 2014
    Date of Patent: October 30, 2018
    Assignee: CORNELL UNIVERSITY
    Inventors: Vladislav M. Sandler, Shahin Raffi
  • Patent number: 10077459
    Abstract: Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.
    Type: Grant
    Filed: May 4, 2016
    Date of Patent: September 18, 2018
    Assignee: GENERAL ELECTRIC COMPANY
    Inventors: John Richard Nelson, Robert Scott Duthie, Erik Leeming Kvam, Wei Gao
  • Patent number: 10053708
    Abstract: A method of delivering a transgene to a cell is provided. The method uses a vector that contains a T-cell receptor alpha locus control region (TCR?LCR) derived gene regulatory cassette having fewer than 5.0-kb. The method delivers the transgene with spatiotemporally specific gene expression and silencing-prevention controls to a cell such that a predetermined subset of progeny cell-types express a gene product from the transgene. Other progeny of the cell diminish, or silence, the expression of the gene product.
    Type: Grant
    Filed: May 11, 2015
    Date of Patent: August 21, 2018
    Assignee: Research Foundation of the City University of New York
    Inventors: Benjamin D. Ortiz, Armin Lahiji
  • Patent number: 10053683
    Abstract: An sortase-mediated intercellular labeling method allowing for tracking ligand-receptor interaction both in vitro and in vivo; and uses thereof for tracking molecule interactions both in vitro and in vivo, identifying modulators of ligand-receptor interaction, identifying potential binding partners of a protein of interest, identifying B cells expressing high affinity B cell receptors to antigens, and identifying the antigen to which a T cell of interest binds.
    Type: Grant
    Filed: October 5, 2015
    Date of Patent: August 21, 2018
    Assignee: Whitehead Institute for Biomedical Research
    Inventors: Giulia Pasqual, Gabriel Victora
  • Patent number: 10047356
    Abstract: Methods for identifying patient specific driver mutations are provided. The methods provided identify specific patient derived markers associated with aberrant signal transduction pathways, in biological samples of a cancer patient.
    Type: Grant
    Filed: January 16, 2014
    Date of Patent: August 14, 2018
    Assignee: NOVELLUSDX LTD.
    Inventors: Yoram Altschuler, Gabi Tarcic
  • Patent number: 10021861
    Abstract: Described herein are immunodeficient non-human animals lacking expression of toll-like receptor 4 (TLR4) by endogenous autogeneic innate immune cells, as well as methods and compositions for engraftment of xenogeneic hematopoietic stem cells in the immunodeficient non-human animal lacking expression of toll-like receptor 4 (TLR4), thereby creating an innate immune system in the animal derived from the xenogeneic hematopoietic stem cells. Further described are immunodeficient mice lacking expression of toll-like receptor 4 by endogenous autogeneic innate immune cells, as well as methods and compositions for engraftment of xenogeneic hematopoietic stem cells in the immunodeficient mouse lacking expression of toll-like receptor 4, thereby creating an innate immune system in the animal derived from the xenogeneic hematopoietic stem cells.
    Type: Grant
    Filed: August 15, 2016
    Date of Patent: July 17, 2018
    Assignees: The Jackson Laboratory, University of Massachusetts
    Inventors: Leonard D. Shultz, Dale L. Greiner, Michael A. Brehm
  • Patent number: 9995730
    Abstract: The present invention relates to the construction of a new hypoxia inducible factor (HIF) responsive reporter gene construct, the genetic constructs and vectors containing the same. Further, the present invention relates to a stable cell line comprising the HIF responsive reporter construct, as well as methods and uses of the inventive constructs and cell lines to identify modulators of HIF activity.
    Type: Grant
    Filed: January 29, 2013
    Date of Patent: June 12, 2018
    Assignee: UNIVERSITI PUTRA MALAYSIA
    Inventors: Norazizah Shafee, Eric J. Stanbridge, Khatijah Yusoff, Sien-Yei Liew
  • Patent number: 9982034
    Abstract: The present disclosure relates to compositions and methods for targeting expression of exogenous genes to platelets. In particular, the present disclosure relates to treatment of hemophilia and other diseases and conditions by targeting expression of exogenous agents (e.g., clotting factors) to platelets.
    Type: Grant
    Filed: October 24, 2013
    Date of Patent: May 29, 2018
    Assignee: PLATELET TARGETED THERAPEUTICS, LLC
    Inventors: David A. Wilcox, Sandra L. Haberichter
  • Patent number: 9958454
    Abstract: The present invention provides methods for constructing peptide construct sets and methods of use of these peptide construct sets in assay systems for peptide analysis, and in particular for use in high throughput peptide analysis. The methods allow for analysis of large sets of peptide constructs in a cost-effective manner, employing molecular biological techniques that are both robust and easily parallelized. Thus, the methods allow for the construction of peptide construct sets encompassing, e.g., the human proteome.
    Type: Grant
    Filed: October 31, 2013
    Date of Patent: May 1, 2018
    Assignee: Prognosys Biosciences, Inc.
    Inventors: Igor A. Kozlov, Mark S. Chee, Petr Capek, David A. Routenberg
  • Patent number: 9944923
    Abstract: Methods for construction of DNA origami nanostructures, as well as for binding, isolation, linking, and deep sequencing information, such as both of TCR alpha and beta CDR3 mRNA, from individual cells within a mixed population of cells without the need for single cell sorting.
    Type: Grant
    Filed: June 9, 2014
    Date of Patent: April 17, 2018
    Assignee: Arizona Board of Regents on Behalf of Arizona State University
    Inventors: Joseph Blattman, Hao Yan, Louis Schoettle, Xixi Wei
  • Patent number: 9938585
    Abstract: The present invention provides a method for screening for iPS cells exhibiting differentiation resistance using a marker identified as lincRNA or mRNA that is specifically expressed in an iPS cell line exhibiting differentiation resistance, and such markers.
    Type: Grant
    Filed: May 19, 2017
    Date of Patent: April 10, 2018
    Assignee: KYOTO UNIVERSITY
    Inventors: Shinya Yamanaka, Kazutoshi Takahashi, Michiyo Koyanagi, Mari Ohnuki
  • Patent number: 9926567
    Abstract: The current invention reports a promoter having the nucleic acid sequence of SEQ ID NO: 02, or SEQ ID NO: 03, or SEQ ID NO: 04, or SEQ ID NO: 06, which is a 5? shortened SV40 promoter with reduced promoter strength especially useful for the limited expression of heterologous polypeptides or selectable markers.
    Type: Grant
    Filed: May 26, 2015
    Date of Patent: March 27, 2018
    Assignee: HOFFMANN-LA ROCHE, INC.
    Inventors: Christian Klein, Erhard Kopetzki
  • Patent number: 9926582
    Abstract: Herein is reported a method for producing a polypeptide comprising the step of incubating (resuspended) prokaryotic cells in a solution comprising about 10 mM to about 95 mM Tris-HCl and about 2 mM to about 6 mM EDTA at a pH value of about 7 to about 10 for about 15 min to about 6 h at about 25° C.
    Type: Grant
    Filed: March 16, 2015
    Date of Patent: March 27, 2018
    Assignee: HOFFMANN-LA ROCHE INC.
    Inventor: Christian Schantz
  • Patent number: 9873900
    Abstract: The present invention is directed to methods and materials for RNA-mediated gene assembly from oligonucleotide sequences. In some embodiments, the oligonucleotides used for gene assembly are provided in an array format. An RNA polymerase promoter is appended to surface-bound oligonucleotides and a plurality of RNA copies of each oligonucleotide are then produced with an RNA polymerase. These RNA molecules self-assemble into a desired full-length RNA transcript by hybridization and ligation. The resulting RNA transcript may then be converted into double strand DNA useful in a variety of applications including protein expression.
    Type: Grant
    Filed: February 8, 2013
    Date of Patent: January 23, 2018
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Cheng-Hsien Wu, Lloyd Smith
  • Patent number: 9868972
    Abstract: This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in vertebrate cells. The components include orthogonal tRNA's, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNA's/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in vertebrate cells are also provided. The present invention provides vertebrate cells with translation components, e.g., pairs of orthogonal aminoacyl-tRNA synthetases (O-RSs) and orthogonal tRNA's (O-tRNA's) and individual components thereof, that are used in vertebrate protein biosynthetic machinery to incorporate an unnatural amino acid in a growing polypeptide chain, in a vertebrate cell.
    Type: Grant
    Filed: August 6, 2015
    Date of Patent: January 16, 2018
    Assignee: Ambrx,Inc.
    Inventors: Feng Tian, Thea Norman, Stephanie Chu
  • Patent number: 9863933
    Abstract: The invention discloses a method for determining the repair activity of NHEJ. In this method, HPRT gene is mutated by using a site-directed gene mutation technology, and plasmid transfection, and 6-TG treatment are performed. By means of the method of the invention, the NHEJ repair activity level of cells can be observed by measuring the cell viability. The method can be used for screening the effects of different drugs and different genes on the NHEJ repair activity.
    Type: Grant
    Filed: August 18, 2015
    Date of Patent: January 9, 2018
    Assignee: SOOCHOW UNIVERSITY
    Inventors: Fenju Liu, Jie Du, Jiahua Yu, Haowen Zhang, Zengfu Shang, Yushuo Zhang
  • Patent number: 9822369
    Abstract: The present invention is related to an L nucleic acid that binds to an SDF-1.
    Type: Grant
    Filed: May 6, 2015
    Date of Patent: November 21, 2017
    Assignee: NOXXON Pharma AG
    Inventors: Werner Purschke, Florian Jarosch, Dirk Eulberg, Sven Klussmann, Klaus Buchner, Christian Maasch, Nicole Dinse
  • Patent number: 9816110
    Abstract: Expression-enhancing nucleotide sequences for eukaryotic expressions systems are provided that allow for enhanced and stable expression of recombinant proteins in eukaryotic cells. Genomic integration sites providing enhanced expression and methods of use thereof are provided for expression of a gene of interest in a eukaryotic cell. Chromosomal loci, sequences, and vectors are provided for enhanced and stable expression of genes in eukaryotic cells.
    Type: Grant
    Filed: October 21, 2015
    Date of Patent: November 14, 2017
    Assignee: REGENERON PHARMACEUTICALS, INC.
    Inventors: Ying Shen, Darya Burakov, Gang Chen, James P. Fandl
  • Patent number: 9809856
    Abstract: The invention encompasses methods and compositions for predicting the risk of metastasis. In particular, the invention encompasses a method for correlating the level of expression of one or more nucleic acid sequences with a risk of metastasis.
    Type: Grant
    Filed: December 12, 2013
    Date of Patent: November 7, 2017
    Assignee: WASHINGTON UNIVERSITY
    Inventor: J. William Harbour
  • Patent number: 9796992
    Abstract: The invention pertains to the field of production of natural products and, in particular, in the field of production of cornexistin and hydroxycornexistin. It provides polynucleotides encoding polypeptides involved in the biosynthesis of cornexistin and hydroxycornexistin as well as vectors and recombinant microorganisms comprising such polynucleotides. Also provided are methods for the production of natural products, in particular methods for the production of cornexistin and hydroxycornexistin, using such polynucleotides and polypeptides encoded therein, as well as vectors and recombinant microorganisms comprising such polynucleotides and polypeptides.
    Type: Grant
    Filed: November 19, 2013
    Date of Patent: October 24, 2017
    Assignee: BASF SE
    Inventors: Oskar Zelder, Birgit Hoff, Hartwig Schröder, Andrea Molt, Holger Hartmann, Klaus Ditrich, Michael Breuer, Rüdiger Reingruber, Jakob Weber