Abstract: The invention concerns novel regulatory elements as well as related vectors and cells. Furthermore, it relates to methods of improving expression of polypeptides from nucleic acids such as cloned genes and to the production of various polypeptides in host cells using said novel regulatory elements. Additionally, the invention relates to uses of said novel regulatory elements as insulators, in gene therapy or for improving host cell lines.
Type:
Grant
Filed:
July 22, 2009
Date of Patent:
June 18, 2013
Assignee:
Boehringer Ingelheim Pharma GmbH & Co, KG
Abstract: The present specification discloses clonal cell lines susceptible to BoNT/A intoxication, methods of producing such clonal cell lines, and methods of detecting Botulinum toxin serotype A activity using such clonal cell lines.
Type:
Grant
Filed:
December 28, 2011
Date of Patent:
June 4, 2013
Assignee:
Allergan, Inc.
Inventors:
Hong Zhu, Joanne Wang, Birgitte P. S. Jacky, D. Dianne Hodges, Fernandez-Salas Ester
Abstract: The present specification discloses clonal cell lines susceptible to BoNT/A intoxication, methods of producing such clonal cell lines, and methods of detecting Botulinum toxin serotype A activity using such clonal cell lines.
Type:
Grant
Filed:
December 28, 2011
Date of Patent:
June 4, 2013
Assignee:
Allergan, Inc.
Inventors:
Hong Zhu, Joanne Wang, Birgitte P. S. Jacky, D. Dianne Hodges, Fernandez-Salas Ester
Abstract: The present specification discloses clonal cell lines susceptible to BoNT/A intoxication, methods of producing such clonal cell lines, and methods of detecting Botulinum toxin serotype A activity using such clonal cell lines.
Type:
Grant
Filed:
December 28, 2011
Date of Patent:
June 4, 2013
Assignee:
Allergan, Inc.
Inventors:
Hong Zhu, Joanne Wang, Birgitte P. S. Jacky, D. Dianne Hodges, Fernandez-Salas Ester
Abstract: A sleeping monitoring system includes a first padding layer. A fluid bladder is beneath the first padding layer. A sensor is in fluid communication with the fluid bladder. The sensor is configured to output a vital sign signal.
Type:
Grant
Filed:
January 7, 2009
Date of Patent:
May 21, 2013
Assignee:
BAM Labs, Inc.
Inventors:
Steven J. Young, Richard V. Rifredi, Yuri Zhovnirovsky
Abstract: The present invention relates to unnatural base pairs of Ds (a 7-(2 thienyl)-3H-imidazo[4,5-b]pyridine-3-yl group) and a Pa derivative (a 2-nitro-1H-pyrrole-1-yl group bearing a substituent having a ?-electron system attached at position 4) that can be replicated with high selectivity/high efficiency, and methods for replicating nucleic acids containing the unnatural base pairs. The present invention also relates to methods for incorporating an unnatural base bearing a functional substituent attached thereto into DNA by a nucleic acid replication reaction. The present invention also relates to methods for replicating and selectively collecting a nucleic acid containing an unnatural base pair from a nucleic acid pool. The present invention also relates to methods for determining a sequence of natural bases in the proximity of an unnatural base in DNA for achieving highly efficient and highly selective replication of a nucleic acid containing the unnatural base.
Abstract: The present invention refers to methods for selectively recognizing a base pair in a DNA sequence by a polypeptide, to modified polypeptides which specifically recognize one or more base pairs in a DNA sequence and, to DNA which is modified so that it can be specifically recognized by a polypeptide and to uses of the polypeptide and DNA in specific DNA targeting as well as to methods of modulating expression of target genes in a cell.
Type:
Grant
Filed:
January 31, 2012
Date of Patent:
April 16, 2013
Inventors:
Ulla Bonas, Jens Boch, Sebastian Schornack, Thomas Lahaye
Abstract: The invention provides transgenomic mitochondria, transmitochondrial cells and organisms, and the materials and methods for making such mitochondria, cells, and organisms.
Abstract: A coated microcarrier for cell culture includes a microcarrier base and a polymeric coating grafted to the base via a polymerization initiator. A method for forming the coated microcarrier includes (i) conjugating a polymerization initiator to the microcarrier base to form an initiator-conjugated microcarrier base; (ii) contacting the initiator-conjugated microcarrier base with monomers; and (iii) activating the initiator to initiate polymerization and graft the polymer to the base.
Type:
Grant
Filed:
July 25, 2012
Date of Patent:
March 26, 2013
Assignee:
Corning Incorporated
Inventors:
Kevin Robert McCarthy, Simon Kelly Shannon, Florence Verrier
Abstract: Disclosed herein are methods for mutagenizing a mammalian gene, the methods involving introducing into a mammalian cell a retroviral vector which includes a splice acceptor sequence, a transcription termination sequence, and retroviral packaging and integration sequences, the introducing step being carried out under conditions which allow the vector to integrate into the genome of the cell. Also disclosed are retroviral vectors for use in these methods as well as methods for the use of mutagenized cells.
Abstract: The disclosure of the present application provides polypeptide sequences and nucleotide sequences coding for the polypeptide sequences of proteins used in the production of APPA-containing peptides. In at least one embodiment of the present disclosure, an isolated nucleic acid is disclosed which comprises a nucleotide sequence encoding a polypeptide having a sequence identity of 60 percent or greater to an amino acid sequence selected from the group consisting of SEQ ID NOS: 2-13, and 15-23.
Type:
Grant
Filed:
January 21, 2011
Date of Patent:
February 12, 2013
Assignee:
University of Illinois at Urbana-Champaign
Inventors:
William W. Metcalf, Wilfred A. van der Donk, Junkal Zhang, Benjamin T. Circello, Svetlana A. Borisova
Abstract: An apparatus comprises a sheath for introducing a catheter into a blood vessel, at least one electrode provided on the sheath, at least one other electrode; and a measuring device to which at least one pair of electrodes are coupled. The measuring device measures the impedance or conduction velocity between the first electrode on the sheath and the second electrode.
Abstract: The present specification discloses clonal cell lines susceptible to BoNT/A intoxication, methods of producing such clonal cell lines, and methods of detecting Botulinum toxin serotype A activity using such clonal cell lines.
Type:
Grant
Filed:
March 12, 2010
Date of Patent:
January 29, 2013
Assignee:
Allergan, Inc.
Inventors:
Hong Zhu, Joanne Wang, Birgitte P. S. Jacky, D. Dianne Hodges, Ester Fernandez-Salas
Abstract: The present invention relates generally to the fields of molecular biology and protein technology. More specifically, the invention concerns signal sequences for the secretion of heterologous polypeptide from bacteria. The invention also concerns recombinant polypeptides and uses thereof.
Abstract: Immortalized avian cell lines containing E1A nucleic acid sequences are useful for the production of viruses and are particularly useful for the production of recombinant viral vectors which can be employed for the preparation of therapeutic and/or prophylactic compositions for the treatment of animals and more particularly humans.
Type:
Grant
Filed:
July 2, 2008
Date of Patent:
January 29, 2013
Assignee:
Transgene S.A.
Inventors:
Philippe Erbs, Marina Kapfer, Nathalie Silvestre
Abstract: Disclosed herein are recombinant tobacco osmotin polypeptides and methods for expressing tobacco osmotin polypeptides in microbial host cells. The recombinant tobacco osmotin polypeptides produced by the methods disclosed herein may be utilized as biocides or as therapeutic agents in medicaments.
Type:
Grant
Filed:
December 17, 2010
Date of Patent:
January 22, 2013
Assignee:
Auburn University
Inventors:
Tung-Shi Huang, Ywh-Min Tzou, Narendra Slngh, Bryan A. Chin
Abstract: This invention relates to industrial production of proteins. More specifically, the invention relates to the res-DHFR surrogate marker, which corresponds to a fusion between DHFR and a protein conferring resistance to a toxic compound or conferring a metabolic advantage. The invention further relates to the use of res-DHFR for screening cells for high expression of a protein of interest. The invention is illustrated by the Puro-DHFR surrogate marker, which corresponds to a fusion between the puromycin N-acetyltransferase and dihydrofolate reductase (DHFR).
Abstract: This invention relates to immortalized avian cells, including those deposited under accession numbers 09070701, 09070702, and 09070703 at the ECACC, and to the use of these cells for the production of viruses. The cells according to the invention are particularly useful for the production of recombinant viral vectors which can be used for the preparation of therapeutic and/or prophylactic compositions for the treatment of animals and more particularly humans.
Type:
Grant
Filed:
July 2, 2010
Date of Patent:
January 22, 2013
Assignee:
Transgene S.A.
Inventors:
Philippe Erbs, Marina Kapfer, Nathalie Silvestre
Abstract: The present invention is directed generally to compositions and methods for expressing recombinant proteins in a mammalian host cell using a co-expressed transcriptional activator. In particular, the invention provides vectors, host cells, and methods of expressing at least one desired polypeptide by transfecting a mammalian host cell with cistrons encoding a transactivator, a desired polypeptide, and an apoptosis-protective protein.