Patents Examined by Changhwa J. Cheu
  • Patent number: 10809266
    Abstract: Provided herein are compositions and methods for the identification of an expression profile in a single cell or population of cells. Kits for use with the disclosed methods are also provided, including antibodies, with a unique molecular identifier and antibody identifier, and primers for amplification of the antibody identifier sequence.
    Type: Grant
    Filed: July 21, 2017
    Date of Patent: October 20, 2020
    Assignee: VERILY LIFE SCIENCES LLC
    Inventors: Ian Peikon, Gary Tong, Daniel Liu, Ci Chu
  • Patent number: 10809264
    Abstract: A concentrated sample having enhanced concentration of the one or more different populations of target rare molecules is incubated with, for each different population of target rare molecules, a particulate or non-particulate affinity agent that comprises a specific binding partner that is specific for and binds to a target rare molecule. The affinity agent comprises a mass spectrometry (MS) label precursor or a first alteration agent, which either facilitates the formation of an MS label from the MS label precursor or releases an entity that comprises the MS label precursor from the affinity agent. The MS label corresponds to one of the populations of target rare molecules. A second alteration agent is employed if the first alteration agent does not facilitate the formation of an MS label from the MS label precursor. MS analysis is used to determine each different MS label.
    Type: Grant
    Filed: May 29, 2015
    Date of Patent: October 20, 2020
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: Michael Pugia, Julia Philip, Karen Marfurt
  • Patent number: 10809250
    Abstract: Methods of detecting an analyte in a sample are provided. In one embodiment, the method comprises forming a partition comprising the sample, a binding agent capable of emitting a signal when bound to the analyte, and a marker capable of identifying the partition; allowing the binding agent to bind to the analyte, if present; and detecting the presence of the analyte in the sample by detecting the signal emitted from the binding agent in the partition.
    Type: Grant
    Filed: August 18, 2016
    Date of Patent: October 20, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Jan zur Megede, George Karlin-Neumann
  • Patent number: 10788484
    Abstract: Provided is a method for quantitative analysis of a sample. The method includes steps of (a) providing a singular marker representative of one or more features of the sample, the sample comprising a population of individual units of the marker; (b) measuring the marker with dot blot analysis, wherein the quantitation result is an absolute amount of the marker's population of individual units in the sample, normalized by the sample volume or by the sample weight; and (c) obtaining an objective determination of the one or more features of the sample based on the quantitation result of the marker. Also disclosed is a reference database and a method to use the reference database for diagnosing cancer in a patient.
    Type: Grant
    Filed: June 28, 2018
    Date of Patent: September 29, 2020
    Assignee: QUANTICISION DIAGNOSTICS INC.
    Inventor: Jiandi Zhang
  • Patent number: 10788482
    Abstract: A point of care immunization system based upon microfluidics and microtitration technologies to rapidly test a patient in order to ascertain an immunization profile so that vaccinations can be administered to address identified gaps. A point of care system comprised of uniquely shaped and color distinguishing sample and test cartridges, with said test cartridges configured to meet healthcare requirements of national governing bodies. A point of care system including an easy access vaccine storage device with indicators to provide data on viability of stored vaccines.
    Type: Grant
    Filed: May 19, 2017
    Date of Patent: September 29, 2020
    Assignee: IMMUNOPROFILE, LLC
    Inventor: Sudip Ghatak
  • Patent number: 10787697
    Abstract: The invention relates to the use of recombinant/semi-synthetic nucleosomes carrying histone and/or DNA modifications as a reference standard for quantification of covalently modified (on the histone proteins or wrapping DNA), variant, or mutant nucleosomes (collectively “modified nucleosomes” or “nucleosome modifications”) from a biological sample. The invention further relates to methods of using the assay to accurately quantify single or combinatorial nucleosome modifications as biomarkers of disease.
    Type: Grant
    Filed: November 8, 2019
    Date of Patent: September 29, 2020
    Assignee: EpiCypher, Inc.
    Inventors: Martis William Cowles, Matthew F. Whelihan, Andrea L. Johnstone, Michael-Christopher Koegh, Zu-Wen Sun, Nathan W. Hall, Matthew R. Marunde
  • Patent number: 10788498
    Abstract: Contemplated test kits and methods for food sensitivity are based on rational-based selection of food preparations with established discriminatory p-value. Particularly preferred kits include those with a minimum number of food preparations that have an average discriminatory p-value of ?0.07 as determined by their raw p-value or an average discriminatory p-value of ?0.10 as determined by FDR multiplicity adjusted p-value. In further contemplated aspects, compositions and methods for food sensitivity are also stratified by gender to further enhance predictive value.
    Type: Grant
    Filed: April 16, 2019
    Date of Patent: September 29, 2020
    Assignee: Biomerica, Inc.
    Inventors: Elisabeth Laderman, Zackary Irani-Cohen
  • Patent number: 10775384
    Abstract: The present invention deals with a method for detecting a protein in a protein sample by amplifying and enhancing small differences between proteins contained in the protein sample. In particular, the present invention uses a cascade of enzymatic modification steps to detect and identify a protein in a protein sample by enhancing small differences between the protein and other proteins contained in the protein sample. Further, the present invention provides a method for distinguishing two proteins having substantially identical or similar amino acid sequences but different protein conformations.
    Type: Grant
    Filed: February 1, 2017
    Date of Patent: September 15, 2020
    Assignee: Sandoz AG
    Inventors: Hans Brandstetter, Julia Hollerweger
  • Patent number: 10775368
    Abstract: A fluidic device includes: a circulation flow path; and a capture part arranged on the circulation flow path and configured to capture a sample substance in a solution and/or a detection part arranged on the circulation flow path and configured to detect a sample substance in a solution.
    Type: Grant
    Filed: August 17, 2017
    Date of Patent: September 15, 2020
    Assignees: The University of Tokyo, NIKON CORPORATION
    Inventors: Takanori Ichiki, Taro Ueno, Ryo Kobayashi, Hirofumi Shiono
  • Patent number: 10768182
    Abstract: The present invention relates to a method for detecting and measuring the presence of cell free mono-nucleosomes and oligo-nucleosomes that contain histone H1 or a histone H1 modification, variant or isoform, and the use of such measurements for the detection and diagnosis of disease.
    Type: Grant
    Filed: October 21, 2016
    Date of Patent: September 8, 2020
    Assignee: Belgian Volition SPRL
    Inventors: Mark Edward Eccleston, Jacob Vincent Micallef
  • Patent number: 10761099
    Abstract: Disclosed herein are methods for detecting protein expression in an individual diagnosed with cystic fibrosis. The methods, in certain aspects, include the steps of obtaining a sample from said individual and detecting expression in said sample of each protein of a protein set. The method may further include the step of determining expression level of one or more proteins of the protein set. The disclosed methods may be used to predict one or more clinical parameters in an individual having cystic fibrosis.
    Type: Grant
    Filed: March 21, 2018
    Date of Patent: September 1, 2020
    Assignee: Children's Hospital Medical Center
    Inventors: Assem Ziady, Rhonda Szczesniak, John Clancy, Cole Brokamp
  • Patent number: 10761097
    Abstract: Herein is reported a method for the detection of free antigen of a multispecific antibody in a sample, whereby the antigen to be detected can be specifically bound by a first binding site of the multispecific antibody, comprising the step of incubating a sample comprising free antigen and multispecific antibody with an anti-idiotypic antibody, which specifically binds to a second binding specificity of the bispecific antibody, which is different from the first binding specificity, whereby the anti-idiotypic antibody is bound to a solid phase.
    Type: Grant
    Filed: March 16, 2018
    Date of Patent: September 1, 2020
    Assignee: Hoffmann-La Roche Inc.
    Inventors: Kay-Gunnar Stubenrauch, Uwe Wessels
  • Patent number: 10753928
    Abstract: Provided is a method for detecting a specific protein, having excellent reliability. The method is characterized by including a mass spectrometry step of detecting the specific protein by mass spectrometry and an immunoassay step of detecting the specific protein by immunoassay using an anti-peptide antibody specifically bonded to the specific protein.
    Type: Grant
    Filed: November 4, 2016
    Date of Patent: August 25, 2020
    Assignee: Morinaga Institute of Biological Science, Inc.
    Inventors: Masahiro Shoji, Tsutomu Honjo, Hideki Okada, Kei Kurihara
  • Patent number: 10746746
    Abstract: The present invention relates to the use of a histone H1 binding agent for detecting, isolating and/or purifying cell free nucleosomes of tumor origin from a biological sample. The invention also describes methods of negative or positive selection using histone H1 binding agents, in order to enrich a sample for cell free nucleosomes of tumor origin.
    Type: Grant
    Filed: October 21, 2016
    Date of Patent: August 18, 2020
    Assignee: Belgian Volition SPRL
    Inventors: Mark Edward Eccleston, Jacob Vincent Micallef
  • Patent number: 10746735
    Abstract: The present invention relates to methods for identifying markers for systemic lupus erythematosus (SLE) and to the markers identified with the aid of this method, which can differentiate between SLE and other autoimmune diseases on the one hand and between different SLE subgroups on the other hand. The invention also relates to panels, diagnostic devices and test kits which comprise these markers, and to the use and application thereof, for example for the diagnosis, prognosis and therapy control in SLE. The invention also relates to methods for screening and validating active substances for application in SLE subgroups.
    Type: Grant
    Filed: February 10, 2015
    Date of Patent: August 18, 2020
    Assignee: Oncimmune Germany GmbH
    Inventors: Angelika Lüking, Peter Schulz-Knappe, Carmen Theek, Petra Budde, Anna Telaar
  • Patent number: 10738345
    Abstract: This invention provides methods for treating diseases associated with elevated p38 mitogen-activated protein kinase activity. Moreover, the invention provides methods for testing a candidate compound for a p38 mitogen-activated protein kinase modifying activity by calculating the level of relocalization of an SMN complex component from the cytoplasm to the nucleus of a cell. Additionally, the invention provides a kit and a system for calculating the same.
    Type: Grant
    Filed: March 26, 2018
    Date of Patent: August 11, 2020
    Assignee: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
    Inventors: Gideon Dreyfuss, Mumtaz Kasim
  • Patent number: 10739347
    Abstract: A bioassay for the detection of multiple target analytes that includes living immunocytes of a plurality of predetermined types; a signal-generating reporter within each immunocyte that is responsive to predetermined changes occurring within the immunocyte and that includes a bioluminescent protein linked to a fluorescent protein; a signal transduction pathway associated with each signal-generating reporter that is operative to induce the predetermined changes within each immunocyte; a detector element associated with the signal transduction pathway that is operative to trigger the signal transduction pathway; an analyte binding element associated with the detector element that is specific to both the detector element and a target analyte; and wherein upon the binding of an analyte binding element to which a target analyte is also bound to the detector element, the detector element triggers the signal transduction pathway causing the predetermined changes to occur within the living biological cell, thereby ca
    Type: Grant
    Filed: August 20, 2018
    Date of Patent: August 11, 2020
    Assignee: Fundamental Solutions Corporation
    Inventors: M. Russell Williams, Joel Lwande, Shengwen Liang, Kyle McQuaid
  • Patent number: 10732178
    Abstract: The present invention is directed to novel assays for detecting target molecules. The assays employ small size, detectably labeled, magnetic nanoparticles associated with a capture molecule. The detection assay is accelerated by applying magnetic field during the assay. The assays of the invention can be used to enhance the efficiency of the detection step in dot blot, Western blot and ELISA.
    Type: Grant
    Filed: January 29, 2014
    Date of Patent: August 4, 2020
    Assignee: BIO-RAD HAIFA LTD.
    Inventors: Shai Nimri, Vered Bronner
  • Patent number: 10725048
    Abstract: Disclosed is a method for detecting a test substance, including a first contact step of bringing the test substance, a capturing body 1 that has binding properties to the test substance and is fixed to a substrate, and a capturing body 2 that has binding properties to the test substance and contains a label into contact with each other in a liquid to arrange at least a part of the capturing body 2 on the substrate; after the first contact step, a first dissociation step of dissociating a part of substances containing the capturing body 2 from the substrate and releasing it into the liquid; after the first dissociation step, a second contact step of bringing the test substance, the capturing body 1 that has binding properties to the test substance and is fixed to a substrate, and the capturing body 2 that has binding properties to the test substance and contains a label into contact with each other in the liquid to arrange at least a part of the capturing body 2 on the substrate; after the second contact step,
    Type: Grant
    Filed: July 13, 2018
    Date of Patent: July 28, 2020
    Assignee: SYSMEX CORPORATION
    Inventors: Kenji Akama, Hiroya Kirimura
  • Patent number: 10725050
    Abstract: The present invention is comprised in the field of glycobiology. In particular, it relates to a method for separating, in biological samples, the fraction bound to or associated with sulfated glycosaminoglycans (GAGs), and the applications thereof in biomedicine, such as for identifying the profile of glycoproteins or the profile of lipids bound to or associated with sulfated GAGs, detecting an alteration in the pattern of glycosylation by sulfated GAGs, identifying biomarkers for the diagnosis, for the prognosis, for monitoring the progression of a disease or of the effect of a therapy, or for identifying compounds suitable for the treatment of a disease. The invention also relates to methods for diagnosing mucopolysaccharidosis and for diagnosing and determining the prognosis of a kidney disease.
    Type: Grant
    Filed: September 9, 2016
    Date of Patent: July 28, 2020
    Assignees: SERVIZO GALEGO DE SAUDE (SERGAS), UNIVERSIDADE DE SANTIAGO DE COMPOSTELA, FUNDACIÓN INSTITUTO DE INVESTIGACIÓN SANITARIA DE SANTIAGO DE COMPOSTELA
    Inventors: Manuela Alonso Sampedro, Victor Álvarez González, Cristóbal Colón Mejeras, Miguel A. García González, Olaya Lamas González