Patents Examined by Changhwa J. Cheu
  • Patent number: 9977026
    Abstract: Identified herein is a novel post-translational modification of Mcm2, wherein the residue of serines 53 and 108 are phosphorylated. DDK phosphorylates Mcm2 at serines 53 and 108 when stimulated by Treslin. DDK is overexpressed in many human cancers, including colorectal cancer, suggesting that monitoring the phosphorylation of Mcm2 at serines 53 and 108 may detect early cancer with high sensitivity and specificity. It was also found that the homologous modification in budding yeast is required for DNA replication, and the modification occurs in cells during active DNA replication only. In an embodiment, the current invention is an antibody specific for human Mcm2 that is phosphorylated at serines 53 and 108. This antibody was found to be overexpressed in colon cancer cell line HCT 116, as well as other cancer cell lines, compared to normal cells.
    Type: Grant
    Filed: July 27, 2016
    Date of Patent: May 22, 2018
    Assignee: The Florida State University Research Foundation, Inc.
    Inventors: Daniel L. Kaplan, Irina Bruck
  • Patent number: 9976135
    Abstract: The present invention relates to a polypeptide consisting of or comprising a variant of human trypsinogen-1, comprising the substitutions: amino acid residue E64 is replaced with an amino acid residue comprising a positively charged side chain, amino acid residue K123 is replaced with an amino acid residue comprising an aliphatic side chain and amino acid residues Y139 and D147 are replaced with a glutamine or asparagine residue, and wherein said variant is further characterized in that: an amino acid residue selected from E16, E17 and E142 is replaced with an amino acid residue comprising an aliphatic side chain, and/or amino acid residue N18 is replaced with a histidine residue, and/or amino acid residue R107 is replaced with a lysine residue, and/or amino acid residue D138 is replaced with an amino acid residue comprising a positively charged side chain, and wherein said variant is cleavable into a polypeptide having a native-like enzymatic activity when compared to human trypsin-1.
    Type: Grant
    Filed: September 25, 2014
    Date of Patent: May 22, 2018
    Inventors: Karin Büttner, Agneta Prasse, Thole Zuchner
  • Patent number: 9964472
    Abstract: One or more methods for sampling gingival metabolites and biomarkers.
    Type: Grant
    Filed: June 29, 2016
    Date of Patent: May 8, 2018
    Assignee: The Procter & Gamble Company
    Inventors: Sancai Xie, John Christian Haught, Cheryl Sue Tansky, Thomas Glenn Huggins, Jr., Donald James White, Jr., Lijuan Li, Malgorzata Klukowska, Angela Marie Fieno, Deepa Ashok Khambe
  • Patent number: 9964538
    Abstract: Disclosed are sensitive methods for detecting a target substance in low concentrations. The method can include immobilizing a target substance to a solid phase; incubating the immobilized target substance with a reporter enzyme detection probe in solution under conditions for forming target: enzyme detection probe complexes; washing the solid phase to remove any unbound reporter enzyme detection probe; incubating the target: enzyme detection probe complex with a reporter enzyme detection probe substrate in substrate reaction solution to generate one or more ionizable products; and detecting one or more of the one or more ionizable products using mass spectrometry (MS).
    Type: Grant
    Filed: November 20, 2015
    Date of Patent: May 8, 2018
    Assignee: YYZ Pharmatech, Inc.
    Inventors: John G. Marshall, Angelica Kirsten Florentinus
  • Patent number: 9958442
    Abstract: A sensor comprising an electronic circuit electrically coupled to a type III-V semiconductor material, for example indium arsenide (InAs) and an antibody contacting the type III-V semiconductor material. The sensor produces measurable N changes in the electrical properties of the semiconductor upon antibody-antigen binding events. Electrical properties measurable by the electronic device may include resistivity, capacitance, impedance, and inductance. A method of detecting an antigen using sensors of the invention. A method of detecting a reaction of an analyte to a stimulus using sensors of the invention. Sensor arrays comprising multiple sensors of the invention.
    Type: Grant
    Filed: February 11, 2010
    Date of Patent: May 1, 2018
    Assignee: Duke University
    Inventors: R. Michael Angelo, April S. Brown, Scott Wolter, William V. Lampert
  • Patent number: 9958461
    Abstract: The present invention relates to methods and kits for detecting the interaction between a first membrane protein and a second protein in mammalian cells as well as to identify molecules that can disrupt protein to protein interactions. The invention relies on the functional reconstitution of an active human ubiquitin by two inactive fragments upon the interaction of two proteins attached through a linker to the inactive fragments. The reconstituted ubiquitin is then cleaved by human ubiquitin proteases resulting in the release of an artificial transcription factor, which in turn activates a reporter gene transcription. Activation of the reporter gene is indicative of the interaction between the two proteins.
    Type: Grant
    Filed: June 10, 2014
    Date of Patent: May 1, 2018
    Inventors: Igor Stagljar, Julia Petschnigg, Bella Groisman
  • Patent number: 9952208
    Abstract: The invention relates to the field of in vitro detection methods using luminescence. Provided is a sensor molecule for detecting an analyte of interest in a sample using bioluminescence resonance energy transfer (BRET), the sensor molecule comprising a proteinaceous moiety tethered to a synthetic regulatory molecule. Also provided is an analytical device comprising a sensor and methods using the sensor molecule.
    Type: Grant
    Filed: July 18, 2013
    Date of Patent: April 24, 2018
    Assignee: Ecole Polytechnique Fédérale de Lausanne
    Inventors: Kai Peter Johnsson, Alberto Schena, Rudolf Griss
  • Patent number: 9945869
    Abstract: Herein is reported a method for the detection of free antigen of a multispecific antibody in a sample, whereby the antigen to be detected can be specifically bound by a first binding site of the multispecific antibody, comprising the step of incubating a sample comprising free antigen and multispecific antibody with an anti-idiotypic antibody, which specifically binds to a second binding specificity of the bispecific antibody, which is different from the first binding specificity, whereby the anti-idiotypic antibody is bound to a solid phase.
    Type: Grant
    Filed: August 1, 2014
    Date of Patent: April 17, 2018
    Inventors: Kay-Gunnar Stubenrauch, Uwe Wessels
  • Patent number: 9946796
    Abstract: The present invention provides methods, databases and devices for establishing the first integration of social behavior with biological phenotypic measurements. In one embodiment, a method for correlating data from a sample database and a survey database is provided. The method comprises obtaining a sample comprising biological molecules from an individual, simultaneously obtaining survey data from the individual, storing the survey data in the survey database, analyzing the sample of biological molecules to determine the composition of biological molecules, storing the data from the composition in the sample database, and correlating the data from the sample database to the data from the survey database.
    Type: Grant
    Filed: May 23, 2013
    Date of Patent: April 17, 2018
    Assignee: IPHENOTYPE LLC
    Inventors: Jay M. Short, Steve Briggs
  • Patent number: 9945854
    Abstract: Autologous bone marrow cells (BMC) are transplanted to a heterologous site in a patient after a sample of the patient's BMC has been tested and found to have a phenotypic profile which meets minimum criteria for transplantation. The phenotypic profile may be obtained by screening a sample of bone marrow cells (BMC) from the patient for the phenotypic profile, such as a CD profile, the phenotype profile may be assessed to determine the likelihood that the BMC will be suitable for transplantation to the heterologous tissue site without enriching particular phenotypic population(s) of the BMC.
    Type: Grant
    Filed: September 1, 2017
    Date of Patent: April 17, 2018
    Assignee: BioCardia Inc.
    Inventors: Peter Altman, Cheryl Wong Po Foo
  • Patent number: 9938523
    Abstract: Methods and compositions for multiplexed protein-protein interaction profiling (e.g., immunoprofiling), based on nucleic acid tagging of polypeptides (e.g., by RNA display) are described. In some embodiments the described compositions and methods utilize a library of prey polypeptide targets linked to prey RNAs encoding them, and a population of bait polypeptides, e.g., a mixture of antibodies, that bind to one or more of the prey polypeptide targets and are used to isolate and identify the bound prey polypeptide targets by amplification of their associated prey RNAs and sequencing of the corresponding cDNAs. In other embodiments the prey polypeptide targets are linked to DNA Bar Codes, which serve as unique identifiers of the tagged polypeptide.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: April 10, 2018
    Assignee: Arizona Board of Regents on Behalf of Arizona State University
    Inventor: Joshua LaBaer
  • Patent number: 9927443
    Abstract: Methods to estimate safety and/or efficacy of therapeutic drugs, which include portable devices for anti-drug antibody (ADA) testing and databases containing anonymized data from humans and/or animal models, and related analyses, are provided. These methods and compositions can be used in various applications, including but not restricted to the following: uniform testing of patients for ADA; selection of therapeutic drug for patient treatment; evaluation of the need to change therapeutic drug or to apply tolerance regimens; selection of patients for clinical trials; comparison of therapeutic drugs marketed for a given disease and also gene therapy; scientific guidance for discovering and/or developing therapeutic drugs; postmarketing surveillance of therapeutic drugs.
    Type: Grant
    Filed: April 5, 2016
    Date of Patent: March 27, 2018
    Assignee: ConquerAb Inc.
    Inventor: Maria D. F. S. Barbosa
  • Patent number: 9921230
    Abstract: This invention relates to compositions and methods for diagnosing and treating concussion/mTBI.
    Type: Grant
    Filed: October 8, 2015
    Date of Patent: March 20, 2018
    Assignee: Rhode Island Hospital
    Inventors: Adam Chodobski, Joanna Szmydynger-Chodobska
  • Patent number: 9910047
    Abstract: Biomarkers relating to insulin resistance and insulin resistance-related disorders are provided, as well as methods for using such biomarkers as biomarkers for insulin resistance, dysglycemia, type-2 diabetes, and cardiovascular disease. In addition, methods for monitoring the respective disorders or conditions of a subject are also provided. Also provided are suites of small molecule entities as biomarkers for insulin resistance, dysglycemia, type-2 diabetes, and cardiovascular disease.
    Type: Grant
    Filed: January 16, 2014
    Date of Patent: March 6, 2018
    Assignee: Metabolon, Inc.
    Inventors: Walter Gall, Jeffery Edmond Cobb, Kirk Lane Pappan
  • Patent number: 9896717
    Abstract: The present invention provides methods of detecting an antigen in a sample comprising contacting the sample with affinity agents to form an antigen-affinity agent-label complex, separating the antigen-affinity agent-label complex from uncomplexed components, partitioning label from the separated sample into multiple partitions, and detecting the presence of the label in one or more partitions. Compositions and kits for detecting an antigen in a sample are also provided.
    Type: Grant
    Filed: May 7, 2014
    Date of Patent: February 20, 2018
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Shawn Hodges, Nick Heredia, Jonathan Petersen
  • Patent number: 9891236
    Abstract: The present disclosure relates to assays and methods for the detection of renal inflammation by measuring the level of P2Y14 and/or UDP-glucose in a sample from a subject, such as a urine sample. The present disclosure also relates to methods for the treatment of renal inflammation by administering a P2Y14 inhibitor.
    Type: Grant
    Filed: November 7, 2014
    Date of Patent: February 13, 2018
    Inventors: Sylvie Breton, Dennis Brown, Anie Azroyan, Virna F. Cortez-Retamozo, Mikael Pittet
  • Patent number: 9891222
    Abstract: The present invention includes a semi-quantitative method for the detection of ENOX2 transcript variants from one or more anti-ENOX2 antibody-binding spots on a blot comprising the steps of: electrophoretically separating proteins from a concentrated blood, serum, or plasma sample from the subject; transferring the electrophoretically separated proteins to a substrate; separating the one or more ENOX2 transcript variants from the one or more anti-ENOX2 antibody-binding spots; and measuring an ENOX2-catalyzed conversion of an ionic silver or gold to a colloidal silver or gold detected by light scattering from the one or more anti-ENOX2 antibody binding spots on the substrate, wherein each of the one or more spots is indicative of the ENOX2 transcript variant. Also provided is the basis for a point of care test to detect ENOX2 transcript variants based on use of colloidal gold or silver complexes with ENOX2 as a rapid simple test for cancer presence.
    Type: Grant
    Filed: May 4, 2017
    Date of Patent: February 13, 2018
    Assignee: Mor-Nuco Enterprises, Inc.
    Inventors: D. James Morre, John Orczyk
  • Patent number: 9885726
    Abstract: This disclosure provides, in one aspect, a method for analyzing a sample from a subject for a biomarker that is indicative of the subject's immune response to ?-glucan. Generally, the method includes obtaining a biological sample from a subject, analyzing the sample for a biomarker anti-?-glucan antibody compared to a reference standard, computing a Relative Antibody Unit (RAU) value for anti-?-glucan antibody in the sample, and identifying the subject as biomarker positive if the RAU value is greater than a predetermined RAU value for the biomarker anti-?-glucan antibody.
    Type: Grant
    Filed: December 1, 2014
    Date of Patent: February 6, 2018
    Assignee: Biothera, Inc.
    Inventors: Nandita Bose, Mary A. Antonysamy, Keith B. Gorden, Richard Walsh, Michael E. Danielson, Peter Maimonis
  • Patent number: 9885708
    Abstract: Methods for preparation of cells for analysis of biomarkers are disclosed. In one aspect, the method for preparation of cells for analysis of biomarkers includes contacting a sample that contains a population of cells with at least one modulating substance at a first temperature, thereby producing a modulated cell population; contacting the modulated cell population with at least one antibody that is directed to a cell surface biomarker at a second temperature that is lower than the first temperature, thereby producing an extracellularly stained cell population; and contacting the extracellularly stained cell population with one or more reagents that fixes and permeabilizes the cells, thereby producing a fixed and permeabilized cell population.
    Type: Grant
    Filed: November 27, 2013
    Date of Patent: February 6, 2018
    Inventor: Jita De
  • Patent number: 9880175
    Abstract: Methods are disclosed for identifying one or more proteins or polypeptides comprised by a sample. The methods comprise determining binding of each polypeptide with respect to each binding pool of a plurality of binding pools, wherein each binding pool comprises one or more probes which bind a structure comprised by a protein or polypeptide. In some aspects, polypeptides can be denatured and separated into individual polypeptide strands and immobilized on a solid support prior to determining binding of the binding pools. A protein, polypeptide or polypeptide strand can be identified by searching, in at least one database, for a protein or polypeptide sequence comprising binding pool targets either identical to or most similar to the binding pool targets comprised by the protein, polypeptide or polypeptide strand to be identified. Kits for identifying proteins, polypeptides and polypeptide strands are also disclosed.
    Type: Grant
    Filed: December 23, 2016
    Date of Patent: January 30, 2018
    Assignee: Washington University
    Inventor: Robi D. Mitra