Patents Examined by Charles Z Constantine
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Patent number: 11753668Abstract: The present invention pertains to a cell culture medium comprising copper as a media supplement, which was shown to control recombinant protein glycosylation and methods of sing thereof. The present invention further pertains to a method of controlling or manipulating glycosylation of a recombinant protein of interest in a large scale cell culture.Type: GrantFiled: February 2, 2022Date of Patent: September 12, 2023Assignee: Biogen MA Inc.Inventors: Fernie Mitchelson, Erik Hughes, Jessica Mondia, Robin Parish Hyde-Deruyscher
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Patent number: 11421255Abstract: A method for enzymatic preparation of fludarabine phosphate, comprising reaction of fludarabine with a high-energy phosphate compound under the action of deoxyribonucleic acid kinase. According to said method, acetate kinase and acetyl phosphate free acid or acetyl phosphate are also added. The technical problems present in the existing processes are successfully addressed by employing the enzymatic process to prepare the fludarabine phosphate. The usage of the high-energy phosphate compound is reduced by means of adding acetate kinase to recycle and regenerate a small amount of the high-energy phosphate compound, thereby reducing the generation of by-products having similar structures to the fludarabine phosphate, enhancing the operation convenience of purification steps in the industrial production of the fludarabine phosphate. The process is environment friendly, the reaction conditions are moderate, the cost is low, and the yield and the purity of the product obtained are high.Type: GrantFiled: January 20, 2016Date of Patent: August 23, 2022Assignee: Zhejiang Hisun Pharmaceutical Co., Ltd.Inventors: Dejin Fu, Yong Yang, Wei Yan, Weijiang Wen, Xiande Gu, Hua Chen, Xiaojun Ma, Ling Zhang, Kaijun Wang
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Patent number: 10912818Abstract: The invention relates to acetylcholine-producing microorganisms for use in the prevention and/or treatment of intestinal diseases, and/or reduction of risks of intestinal diseases, and/or improvement of intestinal health as well as promoting healthy gut flora. The acetylcholine-producing microorganisms may be provided as a pharmaceutical dosage form or as additive to functional food or food supplemental products. Also encompassed is a method for the production of acetylcholine by use of Lactobacilli. Further the invention refers to microbially produced acetylcholine for use in the treatment and/or prevention of intestinal diseases.Type: GrantFiled: August 4, 2017Date of Patent: February 9, 2021Assignee: Ludwig Stocker Hofpfisterei GmbHInventors: Jürgen Mayer, Dirk Haller, Anna Zhenchuk, Thomas Hofmann, Andreas Dunkel, Michael Schemann, Dagmar Krüger
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Patent number: 10808275Abstract: Use of at least one chromogenic and/or fluorogenic phosphatase substrate for the detection and/or enumeration of enterobacteria in a sample likely to contain them, such as a food sample.Type: GrantFiled: April 1, 2014Date of Patent: October 20, 2020Assignee: BIOMÉRIEUXInventors: Marie Cellier, Marie-Pierre Bourguignon, Diane Halimi, Sylvain Orenga
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Patent number: 10772318Abstract: A composition and method for generating reagents and the composition of these reagents for the stabilization and preservation of viability of cancer tissue which has been surgically excised and the suspension and/or termination of apoptosis (cell death) by significant modulation of cell metabolism by low molar concentrations of synergistic chemistries and hormonal growth enhancers while maintaining normal gene expression patterns of the surgically excised tissue.Type: GrantFiled: February 13, 2017Date of Patent: September 15, 2020Assignee: Truckee Applied Genomics, LLCInventor: Tony K. Baker
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Patent number: 10662420Abstract: Methods and devices are provided for pre-treatment of a sample containing microbial cells. In some embodiments, the pre-treatment of the sample is performed via the initial selective lysis, within a sample pre-treatment vessel, of non-microbial cells, such as blood cells, and the subsequent centrifugal separation of the sample to remove the resulting debris and concentrate the microbial cells. An immiscible and dense cushioning liquid may be included for collecting the microbial cells adjacent to the liquid interface formed by the cushioning liquid upon centrifugation of the pre-treatment vessel. After removal of a substantial quantity of the supernatant, resuspension of the collected microbial cells, and re-establishment of the cushioning liquid interface, at least a portion of the remaining suspension may be removed without substantially removing the cushioning liquid. One or more intermediate wash cycles may be performed prior to extraction of the remaining suspension, which provides a “pretreated” sample.Type: GrantFiled: June 2, 2017Date of Patent: May 26, 2020Assignee: QVELLA CORPORATIONInventors: Samad Talebpour, Aye Aye Khine, Robert Maaskant, Tino Alavie
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Patent number: 10385304Abstract: Methods and apparatus for growing photosynthetic organisms lacking Photosystem II (PSII) function using externally supplied electrons shuttled into the organism using redox mediators to improve photosynthetic output and to produce and recover chemicals of interest. By removing PSII, all PAR photons are funneled toward Photosystem I, thereby significantly increasing the theoretical photon utilization efficiency for CO2 fixation, energy storage and the capacity to synthesize valuable chemicals. Additional genetic modification can be performed to insert or enhance specific metabolic pathways to generate products of commercial interest.Type: GrantFiled: December 17, 2015Date of Patent: August 20, 2019Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITYInventors: Justin Flory, Petra Fromme, Willem Vermaas, Bruce Rittman, Cesar Torres, Thomas Moore, Ana Moore
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Patent number: 10077463Abstract: Provided herein are photochemical crosslinkers and photocleavable crosslinkers and their uses in methods for cell selection from cell cultures. The photochemical crosslinkers comprise a fluorescent dye and a radical generator. The photocleavable crosslinkers comprise a photocleavable linker linking two electrophilic groups to each other. Also provided are systems for imaging cells comprising a plurality of cells crosslinked to extracellular matrix proteins using a crosslinker as described, an imaging apparatus, an illuminating apparatus, and software for image processing.Type: GrantFiled: January 14, 2016Date of Patent: September 18, 2018Assignee: President and Fellows of Harvard CollegeInventors: Adam E. Cohen, Miao-Ping Chien
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Patent number: 8557560Abstract: The present invention relates to a novel approach to prevent or treat mastitis both in humans and animals through the use of mammal milk-derived microorganisms obtained from healthy hosts of the homologous species; to those new probiotic microorganisms obtained from milk able to reduce infectious mastitis and the method of screening used for their obtaining; to the use of these probiotic bacteria for the prophylaxis or treatment against mastitis and other diseases; and finally, to compositions comprising these compounds.Type: GrantFiled: June 2, 2008Date of Patent: October 15, 2013Assignee: Puleva Biotech, S.A.Inventors: Rocio Martín Jiménez, Mónica Olivares Martín, Esther Antonia Jiménez Quintana, Maria Luisa Marín Martínez, Saleta Sierra Ávila, Antonio Maldonado Barragán, Virginia Martín Merino, Francesc Blanch Martell, Celina Torre Lloveras, Federico Lara Villoslada, Rebeca Arroyo Rodríguez, Julio Boza Puerta, Jesús Jiménez López, Leónides Fernández Álvarez, Odón Julían Sobrino Abuja, Jordi Xaus Pei, Juan Miguel Rodríguez Gómez, Susana Delgado Palacio
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Patent number: 8198097Abstract: A do-it yourself test kit as a diagnostic agent for the detection of malonyldiadeyde and other aldehydes in the urine, which are formed in the course of the lipid peroxidation process caused by the free radicals. Also disclosed is a process for the preparation of the test, including suggested formulations of antioxidants as body's protectors against oxidative stress to the cells and help to reduce the risk of developing diseases due to the attack of free radicals.Type: GrantFiled: September 21, 2007Date of Patent: June 12, 2012Inventor: Ivo E. Pera
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Patent number: 8173385Abstract: The present invention provides a simple method for the stabilization of a peptide in a biological sample and a reagent for the stabilization, a simple method for the preservation of a biological sample comprising a peptide and a reagent for the preservation, and a method for the accurate measurement of a peptide in a biological sample and a reagent for the measurement. Addition of a saccharide to a biological sample enables the stabilization of a peptide in the biological sample, the preservation of the biological sample comprising a peptide in a stable condition and the accurate measurement of a peptide in the biological sample. As the present invention can stabilize a peptide in a biological sample collected in the clinical examination, the peptide as a marker in the biological sample can be measured accurately in the clinical examination.Type: GrantFiled: July 20, 2006Date of Patent: May 8, 2012Assignee: Kyowa Medex Co., Ltd.Inventors: Norihito Aoyama, Kazuhiko Sasaki, Osamu Kusada