Abstract: Compositions, devices, methods and systems are provided for differential functionalization of a surface of a structure to support biopolymer synthesis. Provided herein are processes which include use of lamps, lasers, and/or microcontact printing to add functional groups to surfaces for the efficient and uniform synthesis of oligonucleic acids.
Type:
Grant
Filed:
November 30, 2016
Date of Patent:
February 20, 2018
Assignee:
TWIST BIOSCIENCE CORPORATION
Inventors:
Bill James Peck, Pierre Indermuhle, Eugene P. Marsh, Andres Fernandez, David Stern
Abstract: The present disclosure relates to compositions and methods based on a fast, efficient chemical reaction for conjugating a pore-forming protein, such as ?-hemolysin, to a biomolecule, such as antibodies, receptors, and enzymes, such as DNA polymerase, and the use of such pore-forming protein conjugates in nanopore devices and methods.
Type:
Grant
Filed:
February 22, 2017
Date of Patent:
February 13, 2018
Assignee:
THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
Inventors:
Jingyue Ju, Zengmin Li, Sergey Kalachikov, Carl Fuller
Abstract: The invention relates to a method and apparatus for detecting elution of a sample from a sample substrate to incubation buffer contained in a sample well while the sample substrate is still within the well. The method comprises measuring light absorption of the contents of the sample well at a predefined wavelength or wavelength range, and determining, based on the absorption measurement, the degree of elution of the sample. According to the invention, a wavelength or wavelength range is used which is absorbed by at least one elutable component of the sample but transmitted by the sample substrate. The invention provides a reliable way of determining the degree of elution of blood samples in neonatal screening, for example.
Abstract: The present invention relates to a composition for aggregating biological sample used for manufacturing paraffin block for microscopic observation of biological sample, a method for preparing paraffin block using the same, and a method for microscopic observation of biological sample using the paraffin block, and particularly to a composition for aggregating biological sample including the first composite containing at least one water-soluble polymer selected from the group comprising polyvinyl alcohol, polyethylene oxide, polyacrylamide, polyvinylpyrrolidon, polyacrylic acid, polyethyleneimine, polyamines, polyamideamine, and polydiallyl dimethylammonium chloride and distilled water; and the second composite containing at least one organic solvent selected from the group comprising alcohol, xylene, and acetone and at least one medical adhesive selected from the group comprising cyanoacrylate, fibrin glue, protein glue, polyurethane, and sealant containing PEG (polyethylene glycol), a method for preparing par
Abstract: The present disclosure provides a method of making a systematic single point mutation in a target nucleic acid and a method of generating a mutational library comprising target nucleic acids with single point mutations. The mutational library comprises target nucleic acids with single point mutations distributed evenly throughout the target nucleic acid.
Type:
Grant
Filed:
May 4, 2016
Date of Patent:
January 16, 2018
Assignees:
WASHINGTON UNIVERSITY, SHRINERS HOSPITALS FOR CHILDREN
Inventors:
Christine Gurnett, Gabriel Haller, Matthew Dobbs, David Alvarado
Abstract: The objective of the present invention is to improve the efficiency of screening/selection of a membrane protein in molecular evolutionary engineering (for example, an enzyme evolutionary method). The above-described objective is achieved by providing a unilamellar liposome comprising: (a) a DNA comprising a promoter sequence, a translational initiation sequence, and a sequence encoding a membrane protein; (b) an RNA polymerase; (c) a ribonucleotide; and (d) a cell-free protein synthesis system. In one aspect of the present invention, the membrane protein is a transporter, and the unilamellar liposome further comprises (e) a factor that binds to a ligand transported by the membrane protein.
Abstract: The present invention relates to a method for quantifying the relative content of a protein in a sample. The present invention also relates to a method for comprising the relative content of a protein in at least two samples.
Type:
Grant
Filed:
December 31, 2011
Date of Patent:
January 2, 2018
Assignee:
BGI TECH SOLUTIONS CO., LTD.
Inventors:
Haiyi Zhao, Dahai Jiang, Bo Wen, Jian Wang, Jun Wang, Huanming Yang, Jin Zi, Yan Ren, Siqi Liu
Abstract: The invention provides methods, kits, compositions, and devices useful for detection of antibodies that bind to Ehrlichia antigens and/or for differentiation of certain Ehrlichia species from others. In particular, the invention provides methods and kits useful for identifying species of Ehrlichia using populations of isolated peptides.
Type:
Grant
Filed:
August 2, 2016
Date of Patent:
December 26, 2017
Assignee:
ABAXIS, INC.
Inventors:
Rajesh K. Mehra, Jeremy D. Walker, Kenneth P. Aron, Dennis M. Bleile, Cristina R. Cuesico, Timothy P. Forsyth
Abstract: Disclosed is a method for measuring the concentration of an analyte or analytes in a solution. Although the methods can be conducted using a number of different assay formats, in one embodiment, the assays are conducted in reaction vessels defined, at least in part, by the distal ends of fiber optic strands.
Abstract: In various embodiments, provided are methods for selecting and formulating compositions for treating and maintaining the quality of skin in a select population, wherein a composition is selected for use in a personal care product based on its demonstrated biological effect to improve skin quality in the population as evidenced by one or more biomarker changes that correlate with improvement as evidenced by one or more objective measurements of skin health in the population.
Type:
Grant
Filed:
January 16, 2011
Date of Patent:
November 7, 2017
Assignee:
The Procter & Gamble Company
Inventors:
Qing Stella, Karl Shiqing Wei, Cynthia Ann Garza, Kenneth Robert Wehmeyer, Rohan Lalith Wimalasena
Abstract: In some embodiments, methods of recovering a sequence-verified target nucleic acid are provided. In some embodiments, such methods may include tagging each member of a nucleic acid library with a set of adaptor sequences; sequencing the tagged members of the nucleic acid library; and recovering the sequence-verified target nucleic acid from the tagged and sequenced members of the nucleic acid library using a dial-out selection method. In certain embodiments, the members of the nucleic acid library may be tagged with a second set of adaptor sequences.
Type:
Grant
Filed:
April 23, 2012
Date of Patent:
November 7, 2017
Assignee:
UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION
Inventors:
Jay Shendure, Jerrod Schwartz, Jacob Kitzman, Rupali Patwardhan, Joseph Hiatt
Abstract: A method for designing circular permuted proteins using two engineered Mu transposons for easy construction of random circular permuted proteins, the two designed transposons being MuCP-ISC (Mu Circular permutation transposon with Integrated Start Codon) and MuCP-ISSC (Mu Circular permutation transposon with Integrated Start and Stop Codon).
Type:
Grant
Filed:
April 14, 2015
Date of Patent:
November 7, 2017
Assignee:
New York University
Inventors:
Jin Ryoun Kim, Brennal Pierre, Vandan Shah
Abstract: A portable plasma based diagnostic apparatus comprising a plasma source for producing energy projectiles at atmospheric pressure, a mass analyzer, a sampling interface for receiving direct sample to be analyzed, the sampling interface being positioned between the plasma source and the mass analyzer, a database containing a library of biomarkers with their associated mass spectra, a processor operatively connected to the plasma source, the mass analyzer and the database. The processor is so configured so as to obtain from the mass analyzer a sample mass spectrum of parent and fragment ions resulting form the collision between the energetic projectiles and the sample, compare the sample mass spectrum with mass spectra in the reference library in order to identify at least one indicator and provide a report based on the at least one identified indicator.
Abstract: Object of the present invention is to develop an artificial synthesis system of various peptides having an azole derivative structure and develop a library of such peptides.
Abstract: The disclosure relates to labeling glycans and glycosphingolipids from undefined mixtures with chemical moieties that emit light when exposed to electromagnetic radiation and uses of these labeled glycans and glycosphingolipids in microarrays for research and diagnostic purposes. In certain embodiments, the disclosure relates to derivatizing glycosphingolipids with a marker.
Type:
Grant
Filed:
November 22, 2011
Date of Patent:
September 26, 2017
Assignee:
Emory University
Inventors:
Richard D. Cummings, David F. Smith, Xuezheng Song
Abstract: A method of analyzing a population of cells is disclosed. In certain embodiments, the method includes i) obtaining an array of cells on a substrate, wherein the cells are labeled with one or more mass tags and are separated from one another, ii) measuring, using secondary ion mass spectrometry (SIMS), the abundance of the one or more mass tags at a plurality of locations occupied by the cells, thereby generating, for each individual cell measured, a set of data, and iii) outputting the set of data for each of the cells analyzed. Also provided herein are systems that find use in performing the subject method. In some embodiments, the system is an automated system for analyzing a population of cells using SIMS.
Type:
Grant
Filed:
March 11, 2016
Date of Patent:
September 19, 2017
Assignee:
The Board of Trustees of the Leland Stanford Junior University
Inventors:
Garry P. Nolan, Sean C. Bendall, Robert M. Angelo
Abstract: A method and system for amplifying non-coding RNA, microRNA, and small polynucleotide sequences through the generation of a pool of signature sequences to the target sequences. The target sequences can be amplified through DNA synthesis, RNA synthesis, or the combination of DNA and RNA synthesis. The amplification of signature sequences provides an efficient and reproducible mechanism to determine the presence or absence of the target miRNAs, to analyze the quantities of the miRNAs, and for miRNA profiling. The method may also be used for screening for unknown non-coding RNAs, including novel miRNAs.
Type:
Grant
Filed:
November 11, 2013
Date of Patent:
September 5, 2017
Assignee:
RESEARCH FOUNDATION FOR MENTAL HYGIENE, INC.
Abstract: Proteins containing a C-terminal thioester are important intermediates in semi-synthesis. Currently there is one main method for the synthesis of protein thioesters that relies upon the use of engineered inteins. The invention involves, in some aspects a method, utilizing Sortase A, for preparation of recombinant proteins containing a C-terminal ?thioester. This new method for double ligatation is useful for synthesizing new or naturally occurring molecules such as a protein thioester.
Type:
Grant
Filed:
May 21, 2013
Date of Patent:
August 15, 2017
Assignee:
Massachusetts Institute of Technology
Inventors:
Bradley L. Pentelute, Xiaoli Liao, Amy E. Rabideau, Jingjing Ling, Rocco L. Policarpo
Abstract: Methods and compositions for making and isolating allosteric DNA binding proteins that bind to one or more allosteric effectors to induce a conformation change in the proteins are provided.
Type:
Grant
Filed:
February 20, 2015
Date of Patent:
August 1, 2017
Assignee:
President and Fellows of Harvard College
Inventors:
Srivatsan Raman, Noah D. Taylor, George M. Church
Abstract: The invention provides a method of identifying biologically active compounds comprising: (a) designing a first library of compounds of formula (1) to scan molecular diversity wherein each compound of the library has at least two pharmacophoric groups R1 to R5 as defined below and wherein compound of the library has same number of pharmacophoric groups; (b) assaying the first library of compounds in one or more biological assay(s); and (c) designing a second library wherein each compound of the second library contains one or more additional pharmacophoric group with respect to the first library; such that the/each component of the first and second library is a compound of formula (1).
Type:
Grant
Filed:
February 20, 2013
Date of Patent:
July 18, 2017
Assignee:
VAST BIOSCIENCE PTY LIMITED
Inventors:
Gerald B. Tometzki, Wim F. Meutermans, Johannes Zuegg