Abstract: A test system having an extended range of measurement and an appropriate procedure for the determination of NAD(P)H or of substrates or enzymes which react to form or consume NAD(P)H in fluids is provided. The test system contains, at one and the same time, several substances acting independently of one another as electron acceptors with respect to NAD(P)H and having different electrochemical potentials. Addition of the test system to the sample solution gives rise to different end products which can be analytically differentiated and which are evaluated visually or by other techniques of measurement.

Abstract: This invention relates to a method for determining fibrinolytic activation in human blood and to a method to distinguish between tissue (e.g. vascular) plasminogen activator and urokinase-like plasminogen activator contributions to fibrinolytic activation.Broadly, the method of the invention relates to detecting the level of plasmin-plasmin inhibitor complexes in blood plasma samples before and after clotting thereof; a greater increase in the complex level in the clotted sample as compared to non-clotted samples indicating the presence of circulating tissue plasminogen activator, with substantially identical levels of increase of complex in both the clotted and unclotted samples indicating the sole presence of urokinase-type plasminogen activator in the sample.

Abstract: An improved immunoassay method, reagent means, test kit, and test device for determining an iodothyronine, e.g., thyroxine (T-4), in a biological fluid, usually serum or plasma, wherein 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid (HMS), or a salt thereof, is employed as a blocking agent for the binding of iodothyronines to thyroxine binding protein (TBP). The present invention is particularly advantageous as applied to homogeneous competitive binding iodothyronine immunoassays employing labels which are participants in enzyme-catalyzed reactions. Such labels include enzyme substrates, coenzymes, enzyme inhibitors, enzyme prosthetic groups, and enzymes.

Abstract: Novel hybridomas, human monoclonal antibodies, and their uses are provided. Specifically CLNH5 is a human-human hybridoma which secretes IgM monoclonal antibodies specific for cervical of carcinomas. The monoclonal antibodies can find use in therapy and diagnosis, both in vitro and in vivo.The hybridoma CLNH5 was deposited at the A.T.C.C. for patent purposes as defined in M.P.E.P. 608.01(p) on Jan. 28, 1983 and given A.T.C.C. Accession No. HB8206.

Abstract: Differentiation, identification and enumeration of sub-populations of lymphocytes including B-cells and T-cells can now be further discriminated into their sub-populations by means of basic orange 21 as a supravital stain to provide distinctive morphologic differences between B-cells, T-suppressor cells, T-helper cells and natural killer (NK) cells without resorting to the use of individual monoclonal antibodies for differentiation of each of the above.The differentiation, identification and enumeration of the foregoing cells can be accomplished by means of both white light absorbance and fluorescence measurements, singly or in sequential combinations.

Abstract: A method for distinguishing multiple subpopulations of a cell sample whereby human natural killer cells subpopulations can be monitored. The method utilizes two monoclonal antibodies identified as anti-Leu-7 and anti-Leu-11.

Abstract: A microbial culture transport apparatus comprising an open top receptacle having a first open-top culture device receiving area for receiving a culture sample and at least a second container retaining area, a cover for the receptacle, and a rupturable container in the second area containing a material for use in maintaining viability of a culture sample. Interengaging detents are provided on the receptacle and cover for releasably retaining the cover in a first position with the top wall of the cover spaced above the bottom of the receptacle to maintain the culture sample and rupturable container in their respective areas without rupturing the container, the interengaging detents being releasable on response to downward pressure on the cover to move down to a second position.

Abstract: A cholinesterase-assaying reagent containing acetylcholine, a thermostable acetate kinase, and adenosine triphosphate is disclosed. This reagent makes it possible to assay cholinesterase, whose activity is clinically important, with good reproducibility and high accuracy.

Abstract: Disclosed is a process for recovering nonsecreted substances produced by cells. The process eliminates some of the high molecular weight contaminants thereby simplifying the purification process. The cells are encapsulated within a semipermeable membrane having properties which permit rapid passage of the relatively low molecular weight substances of interest but retard or prevent passage of higher molecular weight contaminants. The encapsulated cells are suspended in a culture medium and undergo normal cell growth and mitosis. The encapsulated cell culture grows to substantially fill the capsules but not rupture them. The cell membrane is then lysed without disrupting the capsule membrane. The permeability of the capsule membrane is such that the substances of interest diffuse rapidly through the capsule membrane into the extracapsular fluid while the higher molecular weight contaminants and cell fragments are retained within the capsule.

Abstract: Particle reagents, having polyether polyamine linking groups, for use in turbidimetric immunoassays are provided. These linking agents permit ready covalent attachment of compounds of biological interest to polymer particles and lead to immunoassays of improved precision.

Abstract: A process for immobilizing enzyme-containing microbial cells by contacting such cells with an aqueous agar solution containing from about 1.0 to 8.0% sulfate moiety on a weight/weight basis, contacting a solution of inorganic sodium salts with a stream of the cell mixture whereby agar fibers containing cells are formed; and recovering the cell-containing agar fibers produced.

Abstract: Method and apparatus for use in the diagnostic analysis of a liquid specimen through binding assays. The apparatus includes a rigid body having an elongated, shallow well formed in it, with a plurality of elongated strips, preferably in the form of cotton threads, stretched across the well in spaced relationship and generally perpendicular to the well's longitudinal axis. Each thread is coated with a separate binding conjugate such as an antigen or allergen. A cover plate that includes a thin plastic layer and an overlaying thin metallic layer covers the well to form an enclosed chamber having a specific volume. The metallic layer includes a separate elongated aperture in alignment with each coated thread. A liquid specimen is introduced into the chamber through one of two ports, for incubation with the coated threads, after which the specimen is removed and the reaction that occurred on each thread is determined.

Abstract: A member for detecting and/or measuring the level of concentration of a chemical substance in a medium comprises a non-metal substrate bearing a dielectric layer. A detection reactant which is capable of reacting with the substance to be detected is put on the dielectric layer and the assembly is put into the medium to be investigated, whereupon an optically detectable layer of the substance to be detected is formed on the detection reactant.

Abstract: Salmonella typhimurium strain SL1479, ATCC No. 39183 and Salmonella dublin strain SL1438, ATCC No. 39184 are provided which are useful as live vaccines. The vaccines are prepared by introducing at least one modification in a gene involved in at least one, normally at least two, biosynthetic pathways, involving the production of products which are unlikely to be found in the disease susceptible host. The modification results in a gene change which cannot be repaired by a single step e.g. polynucleotide deletions and inversions. Where the aro gene suffers such a change, the resultant auxotrophic mutants required aromatic amino acids, p-aminobenzoic acid and enterochelin or a highly concentrated source of absorbable iron. The auxotrophic mutations have substantially reduced or nonexistent virulence, while retaining the desired immunogenicity to initiate the immunogenic response.

Abstract: A microbial culture apparatus including an outer sealable enclosure for receiving a culture retaining container and a self-contained anaerobic gassing and indicator apparatus which can be selectively activated to provide a gaseous atmosphere around the culture retaining container. The gassing and indicator apparatus includes an open top receptacle having a first rupturable container for producing a gaseous atmosphere, a second rupturable container containing a color indicator, and a catalyst, and a cover for the receptacle arranged to rupture both rupturable containers when the cover is moved to a closed position to activate the gassing and indicating apparatus. The outer enclosure comprises an outer open top receptacle for receiving the culture retaining container and the gassing and indicator apparatus, and a cover which can be moved to a closed position sealing the outer receptacle and activating the gassing and indicating apparatus.