Abstract: An image analysis system is used for the quantitation of nuclear proteins in cell populations. Particularly, the hormonal receptor content of fine needle aspirates of human breast carcinomas are evaluated. Estrogen or progesterone receptors are amplified and visualized in the specimen by a staining technique of the immunoperoxidase type. Monoclonal antibodies specific against the receptor are attached to the receptor sites and are then amplified by a bridging antibody which attaches to the monoclonal antibody and a peroxidase-antiperoxidase complex. A chromagen, diaminobenzidine is combined with the complex and treated with hydrogen peroxide to react with the peroxidase forming an insoluble brown precipitate which marks the receptor sites for optical identification. The specimen is then counterstained with another chromagen, methyl green which is specific to the nucleus of each cell.

Abstract: A method for the diagnosis of whooping-cough is described. The method is carried out such that a sample of body fluid from a patient is incubated with at least a part of a pertussis toxin-sensitive, chromatophore containing fish-scale during a predetermined suitable period of time, that the fish-scale colour then is subjected to a first visual inspection, optionally by means of a microscope, that a substance inducing a colour change in the fish-scales is added, that the fish-scale colour then is subjected to a second visual inspection, optionally by means of the microscope and that the fish-scale colour first observed is compared with the subsequently observed fish-scale colour, the absence of a colour change, or a slight colour change, indicating the presence of pertussis toxin in the body fluid sample.A test kit for carrying the method into effect is described as well.