Abstract: The present disclosure provides methods for obtaining the targeted integration of a DNA molecule into the genome of a host cell using a recombinase. The methods disclosed herein can be used with a variety of host cells, including, for example, dicotyledonous and monocotyledonous plant cells. The present disclosure provides a method for effecting site-specific recombination of DNA within a plant cell, comprising: introducing into the plant cell a target nucleotide sequence comprising a first Int recognition site; introducing into the plant cell a donor nucleotide sequence comprising a second Int recognition site; and introducing into the plant cell an integrase or integrase complex.

Abstract: A novel inbred maize line designated PHENE and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing inbred maize line PHENE with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHENE through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the inbred line PHENE or a trait conversion of PHENE with another maize line. Inbred maize lines derived from inbred maize line PHENE, methods for producing other inbred maize lines derived from inbred maize line PHENE and the inbred maize lines and their parts derived by the use of those methods.

Abstract: A novel inbred maize line designated PHAPT and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing inbred maize line PHAPT with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHAPT through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the inbred line PHAPT or a trait conversion of PHAPT with another maize line. Inbred maize lines derived from inbred maize line PHAPT, methods for producing other inbred maize lines derived from inbred maize line PHAPT and the inbred maize lines and their parts derived by the use of those methods.

Abstract: A novel inbred maize line designated PHEHG and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing inbred maize line PHEHG with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHEHG through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the inbred line PHEHG or a trait conversion of PHEHG with another maize line. Inbred maize lines derived from inbred maize line PHEHG, methods for producing other inbred maize lines derived from inbred maize line PHEHG and the inbred maize lines and their parts derived by the use of those methods.

Abstract: A novel inbred maize line designated PHAR1 and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing inbred maize line PHAR1 with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHAR1 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the inbred line PHAR1 or a trait conversion of PHAR1 with another maize line. Inbred maize lines derived from inbred maize line PHAR1, methods for producing other inbred maize lines derived from inbred maize line PHAR1 and the inbred maize lines and their parts derived by the use of those methods.

Abstract: A novel hybrid maize variety designated 33Y74 and seed, plants and plant parts thereof, produced by crossing Pioneer Hi-Bred International, Inc. proprietary inbred maize lines. Methods for producing a maize plant that comprises crossing hybrid maize variety 33Y74 with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into 33Y74 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. This invention relates to the hybrid seed 33Y74, the hybrid plant produced from the seed, and variants, mutants, and trivial modifications of hybrid 33Y74. This invention further relates to methods for producing maize lines derived from hybrid maize variety 33Y74 and to the maize lines derived by the use of those methods.

Abstract: The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred promoter for the gene encoding Cr1Bio. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method includes stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

Abstract: The present invention provides a method of increasing salt tolerance in a plant by overexpressing a gene encoding a mutant SOS2 protein in at least one cell type in the plant. The present invention also provides for transgenic plants expressing the mutant SOS2 proteins.

Abstract: Constructs and methods are provided for expressing peptides derived from eukaryotic organisms in plant plastids. Constructs have a promoter functional in a plant plastid, a DNA sequence encoding a peptide derived from an eukaryotic organism and a transcription termination region. Other elements include a selectable marker for selection of plant cells comprising a plastid expressing the marker and DNA regions of homology to the genome of the plastid and optionally a ribosome binding site joined to the promoter. By methods using such constructs high levels of eukaryotic peptides, such as mammalian proteins, are produced in a plant cell by growing plant cells under conditions whereby the DNA encoding sequences are expressed to produce eukaryotic peptide in said plastid.

Abstract: This invention relates to a method of transforming plant cells using whiskers on various types of target tissue. And further relates to a method of transforming plant cells using whiskers with an improved agitation method.

Abstract: Methods for the transformation of plants are provided. The methods of the present invention comprise the steps of a) contacting the meristematic tissue of a plant and an area of the plant below the contacted meristematic tissue to a power source, wherein the area below the meristematic tissue is contacted to a positive lead and the meristematic tissue is contacted with a DNA-containing medium which in turn is contacted with a negative lead, and b) applying low amperage current, thus causing the DNA to migrate from the DNA-containing medium into the cells of the meristematic tissue. The transformed plants may be grown to maturity. The mature plants may then self-pollinate and the seed from the plant harvested. Both the parent plant, seed from the parent plant, progeny plants and progeny seeds contain the introduced DNA.

Abstract: Disclosed are isolated transposable elements, or isolated DNA sequences which encode a transposase protein or a portion of a transposase protein. The isolated transposable elements or the isolated DNA sequences are members of the mPing/Pong family of transposable elements. The invention also relates to a purified transposase protein, or peptide fragments thereof, encoded by such DNA sequences. Such transposable elements are useful in applications such as the stable introduction of a DNA sequence of interest into a eukaryotic cell. The sequence information disclosed herein is useful in the design of oligonucleotide primers which are useful for the isolation of related members of the mPing/Pong family of transposable elements, or for the detection of transpositions of the transposable elements.

Abstract: Markers associated with Sclerotinia stem rot resistance are provided. Methods of identifying resistant, and susceptible plants, using the markers are provided. Methods for identifying and isolating QTL are a feature of the invention, as are QTL associated with Sclerotinia stem rot resistance.

Abstract: The present invention relates to a new plant breeding process. The process improves the agronomic performance of crop plants by using genetic material that is also used in classical breeding. Instead of sexually recombining entire genomes at random, as is done in classical breeding, specific genetic elements are rearranged in vitro and inserted back into individual plant cells. Plants obtained through this new plant breeding process do not contain foreign nucleic acid but only contain nucleic acid from the plant species selected for transformation or plants that are sexually compatible with the selected plant species. Plants developed through this new plant breeding process are provided. In particular, potato plants displaying improved tuber storage and health characteristics are provided.

Abstract: Method for the inhibition of carbon flow in the glycolytic direction in a cell by increasing the intracellular availability of trehalose-6-phosphate.

Abstract: The present invention relates to recombinant nucleic acid molecules which contain two or more nucleotide sequences which encode enzymes which participate in the starch metabolism, methods for generating transgenic plant cells and plants which synthesize starch which is modified with regard to its phosphate content and its side-chain structure. The present invention furthermore relates to vectors and host cells which contain the nucleic acid molecules according to the invention, the plant cells and plants which originate from the methods according to the invention, to the starch synthesized by the plant cells and plants according to the invention, and to processes for the preparation of this starch.

Abstract: Disclosed is a method of making transgenic plants. Heterologous DNA is first introduced into a donor plant, plant cell or protoplast, and then moved from the donor to a recipient plant, plant cell or protoplast unaccompanied by any native genomic DNA of the donor. The donor and recipient are chosen that produce unstable progeny or demonstrate preferential segregation or sorting out. The DNA may be inserted randomly or at specific locations in the genome of the recipient plant. Also disclosed are transgenic plants produced by the methods, and plant progeny, plant parts and seeds and seed parts from the plants.

Abstract: A method of controlling cyst nematode population in soybeans by providing a blended composition of soybean varieties. At least one of the soybean varieties in the blend has resistance to a given race spectrum, while another of the soybean varieties in the blend may have resistance to a differing race spectrum or be a susceptible variety. The blend may include 10 to 90% of the first variety and 90 to 10% of the second variety. The blend may consist of three or more soybean varieties.

Abstract: The present invention relates to the modification of galactomannans present in the green coffee bean by reducing the endogenous level of ?-D-galactosidase activity. In particular, the present invention pertains to a plant cell with reduced ?-D-galactosidase activity and to a plant harboring such a plant cell.

Abstract: The present invention provides methods for the conditional or regulated expression of a site-specific recombinase using split intein-mediated protein splicing. This enhances the temporal and tissue-specificity of trait gene expression and allows for fine tuning of expression specificity.