Patents Examined by David Thomas
  • Patent number: 10030023
    Abstract: The invention provides a mercaptoalkylglycoluril represented by the general formula (I): wherein R1 and R2 each independently represent a hydrogen atom, a lower alkyl group, or a phenyl group; R3, R4, and R5 each independently represent a hydrogen atom or a mercaptoalkyl group selected from a mercaptomethyl group, a 2-mercaptoethyl group, and a 3-mercaptopropyl group; and n is 0, 1, or 2. The invention further provides an epoxy resin composition comprising an epoxy resin and the mercaptoalkylglycoluril, and a method for producing a laminate or a multilayer printed circuit board using the same.
    Type: Grant
    Filed: November 28, 2014
    Date of Patent: July 24, 2018
    Assignee: SHIKOKU CHEMICALS CORPORATION
    Inventors: Takeshi Kumano, Takuma Takeda, Noboru Mizobe
  • Patent number: 9885075
    Abstract: The present invention concerns a method of detecting macroions in a liquid medium contained in a space, the method including: a) submitting the liquid medium to a stimulating electrical field to induce formation of aggregates of macroions, the formed aggregates of macroions preferably not including any additional labeling agent, and b) measuring, in a detection zone of the space, spatial and/or temporal fluctuations within the liquid medium of at least one variable depending on the concentration of the macroions in the liquid medium, and c) determining, based on these fluctuations, the presence of the macroions, step c) preferably including processing by a time-dependent or space dependent analysis, more preferably by wavelet analysis, or by autocorrelation the fluctuations measured at step b).
    Type: Grant
    Filed: July 2, 2013
    Date of Patent: February 6, 2018
    Assignees: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, INSTITUT CURIE, UNIVERSITE PIERRE ET MARIE CURIE
    Inventors: Jean-Louis Viovy, François Amblard, Laurent Malaquin, Bastien Venzac, Mohamed Lemine Diakite, Stephanie Descroix, Ismaïl Cisse, Ulrich Bockelmann
  • Patent number: 9869682
    Abstract: The invention relates to a method for detecting the presence of a gynaecological growth, in particular for the diagnosis of endometriosis. The invention also relates to a method of identifying a biomarker for detecting the presence of a gynaecological growth and to biomarkers identified by said method.
    Type: Grant
    Filed: September 1, 2015
    Date of Patent: January 16, 2018
    Assignee: BELGIAN VOLITION SPRL
    Inventors: Jacob Vincent Micallef, Mark Edward Eccleston
  • Patent number: 9862983
    Abstract: This invention covers methods for isothermal amplification of DNA, based on the unexpected discovery that primers having, at some positions, adenine substituted by 2-aminopurine or diaminopurine, guanine substituted by inosine, thymine substituted by 2-thiothymine, and cytosine substituted by N4-ethylcytosine are accepted by enzymes used in standard recombinase polymerase assays (RPA). Further unexpected was the discovery that target nucleotides are efficiently amplified in an RPA-like process (hereinafter abbreviated as simply RPA) using substituted primers. The invention also covers RPA-like processes that use substituted primers tagged with oligonucleotides incorporating nucleotides from artificially expanded genetic information systems (AEGIS).
    Type: Grant
    Filed: June 22, 2015
    Date of Patent: January 9, 2018
    Inventors: Steven Benner, Nilesh Karalkar
  • Patent number: 9863010
    Abstract: The disclosed invention is related to methods, compositions, kits and isolated nucleic acid sequences for targeting Adenovirus nucleic acid. Compositions include amplification oligomers and/or detection probe oligomers. Kits and methods comprise at least one of these oligomers.
    Type: Grant
    Filed: February 1, 2016
    Date of Patent: January 9, 2018
    Inventors: Emily Ziegler, Jessica Townsend
  • Patent number: 9850526
    Abstract: The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. In some aspects, a heterogeneous assay can be performed using a gel. For example, a droplet may be hardened to form a gel, where the droplet contains a cell, DNA, or other suitable species. The gel may be exposed to a reactant, and the reactant may interact with the gel and/or with the cell, DNA, etc., in some fashion. For example, the reactant may diffuse through the gel, or the hardened particle may liquefy to form a liquid state, allowing the reactant to interact with the cell. As a specific example, DNA contained within a gel particle may be subjected to PCR (polymerase chain reaction) amplification, e.g., by using PCR primers able to bind to the gel as it forms. As the DNA is amplified using PCR, some of the DNA will be bound to the gel via the PCR primer.
    Type: Grant
    Filed: May 26, 2015
    Date of Patent: December 26, 2017
    Assignee: President and Fellows of Harvard College
    Inventors: Jeremy Agresti, Liang-Yin Chu, David A. Weitz, Jin-Woong Kim, Amy Rowat, Morten Sommer, Gautam Dantas, George Church
  • Patent number: 9834815
    Abstract: The present teachings generally relate to methods and kits incorporating a discriminating positive control for determining whether a particular microorganism or group of microorganisms are present in a sample, for example but not limited to a food, environmental, agricultural, biopharmaceutical, pharmaceutical, or water sample. According to certain methods, at least part of a starting material, for example but not limited to, a food, environmental, agricultural, biopharmaceutical, pharmaceutical, or water sample can be combined with a culture medium and incubated under conditions suitable for microbial growth followed by extracting microorganism and added control nucleic acids for analysis.
    Type: Grant
    Filed: March 25, 2010
    Date of Patent: December 5, 2017
    Assignee: Life Technologies Corporation
    Inventors: Michael Brewer, Olga Petrauskene, Jen-Kuei Liu, Craig Cummings, Sueh-Ning Liew
  • Patent number: 9822401
    Abstract: The disclosure provides methods and systems for nucleic acid amplification including isothermal nucleic acid amplification.
    Type: Grant
    Filed: April 16, 2015
    Date of Patent: November 21, 2017
    Assignee: GENAPSYS, INC.
    Inventor: Florian Oberstrass
  • Patent number: 9822403
    Abstract: A non-contact system for sorting monodisperse water-in-oil emulsion droplets in a microfluidic device based on the droplet's contents and their interaction with an applied electromagnetic field or by identification and sorting.
    Type: Grant
    Filed: May 19, 2014
    Date of Patent: November 21, 2017
    Assignees: Lawrence Livermore National Security, LLC, The Regents of the University of California
    Inventors: Neil Reginald Beer, Abraham Lee, Andrew Hatch
  • Patent number: 9822396
    Abstract: Methods compositions and kits are provided for performing a chromatin or chromosome conformation capture assay in partitions.
    Type: Grant
    Filed: February 13, 2015
    Date of Patent: November 21, 2017
    Inventors: Claudia Litterst, Svilen Tzonev, Jeremy Agresti
  • Patent number: 9822405
    Abstract: The present invention provides methods, reagents and kits for carrying out a variety of assays suitable for analyzing polynucleotides or samples that include an amplification step performed in a multiplex fashion. Also provided are methods for analyzing and improving the efficiency of amplification and for carrying out gene expression analysis.
    Type: Grant
    Filed: June 26, 2015
    Date of Patent: November 21, 2017
    Assignee: APPLIED BIOSYSTEMS, LLC
    Inventors: Mark Andersen, David Ruff
  • Patent number: 9816121
    Abstract: The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. In some aspects, a heterogeneous assay can be performed using a gel. For example, a droplet may be hardened to form a gel, where the droplet contains a cell, DNA, or other suitable species. The gel may be exposed to a reactant, and the reactant may interact with the gel and/or with the cell, DNA, etc., in some fashion. For example, the reactant may diffuse through the gel, or the hardened particle may liquefy to form a liquid state, allowing the reactant to interact with the cell. As a specific example, DNA contained within a gel particle may be subjected to PCR (polymerase chain reaction) amplification, e.g., by using PCR primers able to bind to the gel as it forms. As the DNA is amplified using PCR, some of the DNA will be bound to the gel via the PCR primer.
    Type: Grant
    Filed: March 3, 2017
    Date of Patent: November 14, 2017
    Assignee: President and Fellows of Harvard College
    Inventors: Jeremy Agresti, Liang-Yin Chu, David A. Weitz, Jin-Woong Kim, Amy Rowat, Morten Sommer, Gautam Dantas, George Church
  • Patent number: 9809846
    Abstract: The invention provides compositions comprising rolling circle amplification sequences and hairpin sequences specifically designed for the accurate and highly sensitive detection of one or more analyte sequences. The invention further provides kits comprising them and methods for their use.
    Type: Grant
    Filed: December 30, 2014
    Date of Patent: November 7, 2017
    Assignee: Yissum Research Development Company of The Hebrew University Of Jerusalem Ltd.
    Inventors: Itamar Willner, Fuan Wang, Chun-Hua Lu, Xiaoqing Liu, Lina Freage
  • Patent number: 9809841
    Abstract: The present invention provides a method of detecting nucleotide sequence differences between two nucleic acid samples. The method employs a comparative genomic hybridization (CGH) technique to analyze the sequence differences between the samples. This method permits the identification of small sequence differences (e.g., sequence divergence of 1% or less) in nucleic acid samples of high complexity (e.g., an entire genome).
    Type: Grant
    Filed: April 20, 2016
    Date of Patent: November 7, 2017
    Assignee: The Regents of the University of California
    Inventors: Donna G. Albertson, Daniel Pinkel, Yevgeniya Fridlyand, Bing Huey, Antoine Snijders
  • Patent number: 9803188
    Abstract: The present disclosure relates to systems and methods for nucleic acid isolation. In particular, the present disclosure provides systems and methods for isolating nucleic acids from aqueous samples (e.g., blood or urine).
    Type: Grant
    Filed: December 21, 2012
    Date of Patent: October 31, 2017
    Assignee: IBIS BIOSCIENCES, INC.
    Inventors: Mark W. Eshoo, Christopher Crowder
  • Patent number: 9797008
    Abstract: In relation to an automated nucleic acid processor and an automated nucleic acid processing method using a multi function dispensing unit, processing involving extraction and amplification of the nucleic acid, can be consistently, quickly and efficiently conducted at a low cost with the use of a multi function dispensing unit, while saving user's trouble without expanding the scale of the device.
    Type: Grant
    Filed: May 8, 2015
    Date of Patent: October 24, 2017
    Assignee: UNIVERSAL BIO RESEARCH CO., LTD.
    Inventor: Hideji Tajima
  • Patent number: 9791409
    Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. A plurality of smaller flow cells is employed, each with a relatively small area to be imaged, in order to provide greater flexibility and efficiency.
    Type: Grant
    Filed: May 11, 2015
    Date of Patent: October 17, 2017
    Assignee: Intelligent BioSystems, Inc.
    Inventors: Steven Gordon, Thomas Hagerott, Edmund Golaski, Jerzy Olejnik
  • Patent number: 9790540
    Abstract: The present innovation provides methods and kits that enable rapid and efficient dual end-tagging of RNA to prepare libraries for analysis by applications such as next-generation RNA sequencing, qPCR, microarray analysis, or cloning. The methods do not require time-consuming and inefficient gel-purification steps that are common to methods known in the art. In addition, the present invention provides methods and kits for rapid, high-throughput enzymatic preparation of 5?-activated, 3?-blocked DNA oligonucleotides from standard, single-stranded DNA oligonucleotides.
    Type: Grant
    Filed: September 13, 2013
    Date of Patent: October 17, 2017
    Assignee: EPICENTRE TECHNOLOGIES CORPORATION
    Inventors: Ramesh Vaidyanathan, Scott Kuersten, Ken Doyle
  • Patent number: 9777315
    Abstract: The present invention relates to a proximity probe based detection assay (“proximity assay”) for an analyte in a sample, specifically a proximity probe extension assay (PEA), an in particular to an improvement in the method to reduce non-specific “background” signals, wherein the improvement comprises the use in such assays of a component comprising 3? exonuclease activity, said method comprising: (a) contacting said sample with at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte; (b) allowing the nucleic acid domains of the proximity probes to interact with each other upon binding of said proximity probes to said analyte, wherein said interaction comprises the formation of a duplex; (c) contacting said sample with a component comprising 3? exonuclease activity; (d) extending the 3? end of at least one nucleic acid domain of said duplex to generate an extension product, wherein
    Type: Grant
    Filed: January 30, 2012
    Date of Patent: October 3, 2017
    Assignee: OLINK PROTEOMICS AB
    Inventors: Simon Fredriksson, Martin Lundberg, Anna Eriksson, Emma Rennel-Dickens
  • Patent number: 9771615
    Abstract: Disclosed is a method for sequencing a polynucleotide analyte comprising: •a. generating a stream of droplets containing a single nucleotide wherein the order of single nucleotides in the droplet stream corresponds to the sequence of nucleotides in the analyte; •b. introducing into each droplet a plurality of biological probe types each type comprising a different label in an undetectable state and being adapted to capture a different single nucleotide; •c. causing the single nucleotide contained in the droplet to bind to its complementary probe and •d. causing the label to be released from the probe that has bound the nucleotide in a detectable state. The probe is a dumbbell shaped probe comprising fluorescent donor and quencher labels and a single nucleotide gap. After gap repair by a polymerase and a ligase, a restriction enzyme recognition site is cleaved by a restriction enzyme, followed by exonuclease digestion to release the labels.
    Type: Grant
    Filed: October 4, 2013
    Date of Patent: September 26, 2017
    Assignee: BASE4 INNOVATION LTD
    Inventors: Cameron Alexander Frayling, Barnaby Balmforth, Bruno Flavio Nogueira de Sousa Soares, Thomas Henry Isaac, Boris Breiner, Alessandra Natale, Michele Amasio