Abstract: A method for diagnosing hyperhomocysteinemia by molecular genetic means is disclosed.

Abstract: A method of cleaning probe cards provided with probe tips, in which method the tips are cleaned of a contaminating material by exposing the tips to a cleaning solution by dipping in the solution such that only their ends touch the cleaning solution.

Abstract: A method of identification of differentially expressed messenger RNA (mRNA) which consists of synthesizing from a set of sequences of mRNA sets of fragments of complementary DNA (cDNA), which are separated with the aid of gel electrophoresis and the pictures of separation of the cDNA from different types of cells are compared and fragments with the aid of restriction nucleases. A method of cloning of differentially expressed mRNAs consists of synthesizing from sets of sequences of MRNAs from different types of cells sets of fragments of complementary DNA (cDNA) which are separated with the aid of gel electrophoresis, the pictures of the separation of the cDNA from different types of cells are compared, fragments of cDNA with different signal intensities are separated from the gel, amplified with the aid of a polymerase chain reaction and clones to a plasmid or phage vector.

Abstract: The present invention concerns a mixture of at least one monoester, at least one methyl ester, at least one olefinic hydrocarbon, along with other optional ingredients, which mixture is useful in cleaning organic residues, particularly inks, from various surfaces.

Abstract: Provided are nucleic acid molecules which comprise at least a portion of a polymorphic genetic marker for determining whether a canine has a mutated progressive rod-cone degeneration disease (prcd) gene locus in one or both alleles. The genetic markers are located on canine chromosome 9 in a genomic region identified as the prcd-informative region. A method for making genetic markers representing polymorphic sequences located in the prcd-informative region of canine chromosome 9 comprises isolating a polymorphic DNA sequence in the prcd-informative region, using oligonucleotides complementary with at least a portion of the sequence to amplify the sequence, and analyzing canine chromosome 9 in a prcd-informative pedigree for the ability of the polymorphic sequence to co-segregate with the prcd gene locus by a linkage test.

Abstract: The invention relates to the isolation of a nucleic acid molecule which encodes an esophageal cancer associated antigen. Also a part of the invention is the antigen itself, and the uses of the nucleic acid molecule and the antigen.

Abstract: Internal Transcribed Spacer (ITS) DNA sequences from the ribosomal RNA gene region are described for different species and strains of Helminthosporium carbonum, Helminthosporium turcicum, Helminthosporium maydis, Cercospora zeae-maydis, Kabatiella zeae and Puccinia sorghi. Specific primers from within these sequences are identified as being useful for the identification of the fungal isolates using PCR-based techniques.

Abstract: The invention involves isolated nucleic acid molecules which encode antigens which have been associated with renal carcinoma. The molecule having the sequence of SEQ ID NO: 1 is exemplary. Cell lines and expression vectors using these nucleic acid molecules are also a feature of the invention.

Abstract: The present invention relates to new nucleotide sequences coding for variable regions of the .alpha. chains of human T lymphocyte receptors, corresponding peptide segments and the diagnostic and therapeutic uses.

Abstract: The present invention provides a method of clinically differentiating the presence of Staphylococcus aureus in isolated samples, comprising the step of: analyzing the hybridization profile observed in Southern blots utilizing DNA probes for genes selected from the group consisting of the cna gene, the fnbA gene, the fnbB gene, the hlb gene, the cna-up gene, the pcp gene, the pcp12 gene and the pcp34 gene. Also provided is a kit for clinically differentiating the presence of Staphylococcus aureus in isolated samples, comprising: (1) restriction enzymes to digest genomic DNA contained in said samples; and (2) DNA probes for genes selected from the group consisting of the cna gene, the fnbA gene, the fnbB gene, the hlb gene, the cna-up gene, the pcp gene, the pcp12 gene and the pcp34 gene.

Abstract: There is provided an oligonucleotide for specifically detecting rice plants resistant to stripe disease, said oligonucleotide comprising a sort of nucleic acid sequence 5'-CAGACCGACC-3' SEQ ID NO: 1 and being capable of amplifying a specific DNA fragment of stripe disease resistant rice. There is also provided a method for specifically detecting rice plants resistant to stripe disease, said method comprising the steps of (1) isolating a genomic DNA from rice leaves; (2) amplifying a DNA fragment by polymerase chain reaction; (3) detecting stripe disease resistant rice plants by an agarose electrophoresis.

Abstract: An improved nucleic acid hybridization process is provided which employs a modified oligonucleotide and improves the ability to discriminate a control nucleic acid target from a variant nucleic acid target containing a sequence variation. The modified probe contains at least one artificial mismatch relative to the control nucleic acid target in addition to any mismatch(es) arising from the sequence variation. The invention has direct and advantageous application to numerous existing hybridization methods, including, applications that employ, for example, the Polymerase Chain Reaction, allele-specific nucleic acid sequencing methods, and diagnostic hybridization methods.

Abstract: A process and an apparatus for continuously and efficiently producing high protein foodstuff such as cheese curd, by continuously sprinkling and pouring a protein solution such as cow's milk or soybean milk from above onto the water surface of a warm water bath through a nozzle having multiple holes. A constant amount of warm water kept at a fixed temperature is supplied to the water bath. This process converts the protein solution into curd useful in producing various types of cheese.

Abstract: The invention is based on the discovery that adults having a genotype comprising a hemoglobin .alpha.-gene deletion are significantly more likely to be hypertensive than adults having a normal (.alpha..alpha./.alpha..alpha.) gentoype. The invention provides an improved method for determining a human subject's genotype at the .alpha.-gene loci; a method of screening a human subject for an increased potential of developing hypertension and other blood-related disorders; and provides an apparatus/kit for screening a human subject for a risk of developing hypertension and other blood-related disorders.

Abstract: Primers and methods for adapter-mediated multiplex amplification of the IS6110 insertion element of Mycobacterium tuberculosis (M.tb) and the 16S ribosomal gene of Mycobacterium tuberculosis, useful for simultaneously detecting and/or identifying species of the M. tuberculosis complex and other clinically relevant Mycobacterium species. Multiplex Strand Displacement Amplification (SDA) is used in a single amplification reaction which is capable of simultaneously identifying M. tuberculosis and providing a screen for substantially all of the clinically relevant species of Mycobacteria. Also disclosed are methods for adapter-mediated multiplex amplification of multiple target sequences and a single internal control sequence for determination of sample efficacy or quantitation of the targets.

Abstract: Nucleic oligomer probes useful for detection of HGBV in test samples. Also provided are assays which utilize these probes and test kits which contain these oligomer probes.

Abstract: The gene responsible for an autosomal dominant con-rod retinal dystrophy is identified, TULP2. The genes are used to produce the encoded protein; in screening for compositions that modulate the expression or function of TULP2 protein; and in studying associated physiological pathways.

Abstract: Specific mutations in the connexin-32 gene that are associated with X-linked Charcot-Marie-Tooth (CMT) disease are disclosed. Methods of diagnosing X-linked CMT disease are also disclosed. Methods include hybridization analysis, such as Southern or Northern analysis, which use hybridization of mutant connexin-32 nucleic acid probes to connexin-32 genes; direct mutation analysis by restriction digest; sequencing of the connexin-32 gene; hybridization of an allele-specific oligonucleotide with amplified genomic DNA; or identification of mutant connexin-32 proteins. Mutant connexin-32 nucleic acid probes are also disclosed. The mutant connexin-32 nucleic acid probes have a mutation in at least one of the following codons: 12, 26, 28, 44, 75, 86, 100, 103, 107, 124, 142, 154, 157, 160, 161, 172, 179, 181, 183, 185, 187, 198, 204, 205, 219, 230 and 235.

Abstract: A new approach is proposed for sequencing by hybridization (SBH), which uses interaction to dramatically reduce the number of oligonucleotides used for de novo sequencing of large DNA fragments, while preserving the parallelism which is the primary advantage of SBH. In particular, a series of rounds is performed, starting from an initial fixed oligonucleotide array, of hybridizing a target sample against an array, and then designing a new oligonucleotide array in response to the results of the rounds to date, until the sequence is determined.