Patents Examined by Dianne Rees
  • Patent number: 6720410
    Abstract: The invention relates to novel nucleic acid fragments encoding polypeptides which are capable of mediating contact between enamel and cell surface. The invention also relates to expression vectors containing the nucleic acid fragments according to the invention for production of the protein, organisms containing said expression vector, methods for producing the polypeptide, compositions comprising the polypeptides, antibodies or antibody fragments recognizing the polypeptides, and methods for treating various hard tissue diseases or disorders.
    Type: Grant
    Filed: September 13, 2001
    Date of Patent: April 13, 2004
    Assignee: Biora AB
    Inventors: Radim Cerny, Ivan Slaby, Lars Hammarström, Tilmann Wurtz, Cheng Dan Fong
  • Patent number: 6100025
    Abstract: Disclosed are methods for detecting mammalian genes encoding proteins which can function in microorganisms, particularly yeast, to modify, complement, or suppress a genetic defect associated with an identifiable phenotypic alteration or characteristic in the microorganism. Disclosed also are mammalian DNA sequences cloned by the above method, as well as polypeptide products of the expression of the DNA sequences in procaryotic or eucaryotic host cells and antibody substances which are specifically immunoreactive with said expression products. More specifically, the present invention relates to methods for cloning mammalian genes which encode products which modify, complement or suppress a genetic defect in a biochemical pathway in which cAMP participates or in a biochemical pathway which is controlled, directly or indirectly, by a RAS-related protein, to products (RNA, proteins) encoded by the mammalian genes cloned in this manner, and to antibodies which can bind the encoded proteins.
    Type: Grant
    Filed: March 1, 1994
    Date of Patent: August 8, 2000
    Assignee: Cold Spring Harbor Laboratory
    Inventors: Michael H. Wigler, John J. Colicelli
  • Patent number: 5985550
    Abstract: A method for the detection of a polynucleotide target sequence is described. The method involves the formation of a covalent or non-covalent bonded pair of nucleotide sequences formed in response to a target polynucleotide sequence, adding nucleotide sequence specific binding proteins each capable of binding one member of the pair of nucleotide sequences, and detecting the specific binding proteins complexed to the pair of nucleotide sequences.
    Type: Grant
    Filed: May 2, 1995
    Date of Patent: November 16, 1999
    Assignee: Behringwerke AG
    Inventors: Thomas C. Goodman, Edwin F. Ullman
  • Patent number: 5955125
    Abstract: The present invention relates to a confectionary holder which simulates the sound of rain as the holder is vertically positioned. The holder comprises a transparent tube having ends, two sides, and at least two sets of indents directed inward toward the center of the tube and one set of indents per side of the tube. Each indent has a peak, a peak depth, a sloping upper face, a sloping lower face, and between each indent is a space length. The peaks of said indents alternate from one side to the other side along the tube length. The angle of incline of each indent face and the length of the space between each indent are selected to allow rigid items, e.g., candy or gum, when placed in the holder, to create the sound of rain as the confectionary pieces flow downward dropping onto each indent face, sliding off each face, and moving from side to side from one end of the holder to the other end when the holder is vertically positioned.
    Type: Grant
    Filed: July 17, 1996
    Date of Patent: September 21, 1999
    Assignee: Oddzon/Cap Toys, Inc.
    Inventors: Michael G. Hoeting, Sean T. Mullaney
  • Patent number: 5914228
    Abstract: The methods and compositions of this invention provide a fast, accurate means for directly determining the presence and quantity of HCV in a sample, and thus in a patient, through hybridization to patient samples using a probe specific for HCV. They allow detection of both acute and chronic HCV infection. These methods and compositions also allow for the effective monitoring of therapeutic procedures for treating HCV infection.
    Type: Grant
    Filed: November 8, 1994
    Date of Patent: June 22, 1999
    Assignee: Cedars-Sinai Medical Center
    Inventors: John M. Vierling, Ke-Qin Hu
  • Patent number: 5885772
    Abstract: The invention includes methods and materials, including probes, to be used as diagnostic tools for detecting anencephaly. Such methods may be practiced by both manual and automated means, and in the instance of the latter, suitable equipment for such automated performance is also contemplated. Also included within the invention are mouse embryos null for a gene encoding a protein kinase C substrate which binds calcium-calmodulin and regulates cell movement and membrane traffic, and cells derived from such embryos.
    Type: Grant
    Filed: March 16, 1995
    Date of Patent: March 23, 1999
    Assignee: The Rockefeller University
    Inventors: Alan A. Aderem, Jianmin Chen, Sandy Chang
  • Patent number: 5882857
    Abstract: The present invention relates to an improvement in a method for amplifying a target sequence of a target polynucleotide. The method comprises combining a sample suspected of containing the target polynucleotide with reagents for amplifying the target sequence and subjecting the combination to conditions wherein the target sequence if present is amplified. The present improvement comprises including in the combination a control oligonucleotide and a control polynucleotide that has a sequence that is hybridizable with the control oligonucleotide. When the control oligonucleotide is bound to the control polynucleotide, the ability of a primer to chain extend along the control polynucleotide is reduced. Optionally, the control oligonucleotide is part of the control polynucleotide. The method finds particular application in the area of nucleic acid amplification and detection.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: March 16, 1999
    Assignee: Behringwerke AG
    Inventors: Linda M. Western, Samuel J. Rose, Edwin F. Ullman
  • Patent number: 5874217
    Abstract: Methods of genotyping canines by analysis of polymorphisms in the number of microsatellite DNA repeats are provided. The internal repeat sequence is amplified by the use of specific primers. The number of repeats, and therefore the distance between the primers, is highly variable in a population, thereby providing an allelic marker for the locus. The combined information from multiple loci provides a means of distinguishing individuals, even among inbred dog breeds, for parentage testing, forensic testing and analysis of individuals relatedness.
    Type: Grant
    Filed: March 27, 1996
    Date of Patent: February 23, 1999
    Assignee: The Perkin-Elmer Corporation
    Inventors: Joy Halverson, Jan Dvorak, Tamara Stevenson
  • Patent number: 5871697
    Abstract: This invention provides methods by which biologically derived DNA sequences in a mixed sample or in an arrayed single sequence clone can be determined and classified without sequencing. The methods make use of information on the presence of carefully chosen target subsequences, typically of length from 4 to 8 base pairs, and preferably the length between target subsequences in a sample DNA sequence together with DNA sequence databases containing lists of sequences likely to be present in the sample to determine a sample sequence. The preferred method uses restriction endonucleases to recognize target subsequences and cut the sample sequence. Then carefully chosen recognition moieties are ligated to the cut fragments, the fragments amplified, and the experimental observation made. Polymerase chain reaction (PCR) is the preferred method of amplification.
    Type: Grant
    Filed: October 24, 1995
    Date of Patent: February 16, 1999
    Assignee: CuraGen Corporation
    Inventors: Jonathan Marc Rothberg, Michael W. Deem, John W. Simpson
  • Patent number: 5871920
    Abstract: Novel gene present in interval 6E of the distal portion of the long arm of the human Y chromosome, whose alteration is associated with reduced sperm count. Methods of diagnosis and treatment utilizing said gene, and antibodies that bind to the protein encoded by said gene.
    Type: Grant
    Filed: July 31, 1996
    Date of Patent: February 16, 1999
    Assignee: Whitehead Institute for Biomedical Research
    Inventors: David C. Page, Renee Reijo
  • Patent number: 5871917
    Abstract: The present invention provides Methyl- (or Mutant-) Differential Display (MDD) methods and nucleic acid probes for detecting mutations and the methylation patterns of nucleic acids. The methods of the present invention are particularly useful for detecting and isolating genomic DNA fragments which are near coding and regulatory regions of genes and which are differentially mutated or methylated relative to the corresponding DNA from normal cells. Genes are frequently not methylated in the cells where they are expressed but are methylated in cell types where they are not expressed. Moreover, tumor cell DNA is frequently methylated to a different extent and in different regions than is the DNA of normal cells. The present invention is used for identifying which regions of the genome are methylated or mutated in different cell types, including cancerous cell types.
    Type: Grant
    Filed: May 31, 1996
    Date of Patent: February 16, 1999
    Assignee: North Shore University Hospital Research Corp.
    Inventor: Hao-peng Xu Duffy
  • Patent number: 5869240
    Abstract: Integrated methods and apparatus for sequencing or identifying polymers by mass spectrometry with a statistical certainty. The methods involve integrating data obtained by mass spectrometry analysis of a series of polymer fragments and statistically comparing said data with hypothetical data corresponding to known sequences or identities.
    Type: Grant
    Filed: May 19, 1995
    Date of Patent: February 9, 1999
    Assignee: PerSeptive Biosystems, Inc.
    Inventor: Dale H. Patterson
  • Patent number: 5866325
    Abstract: Disclosed are DNA sequences encoding novel DNA binding proteins implicated in regulation of early stages of cell growth. Illustratively provided are human and mouse origin DNA sequences encoding early growth regulatory ("Egr") proteins which include "zinc finger" regions of the type involved in DNA binding. Also disclosed is a detailed analysis of the structure and function of the early growth regulatory protein, Egr-1, delineating independent and modular activation, repression, DNA-binding, and nuclear localization activities. Also disclosed are immunological methods and materials for detection of Egr proteins and hybridization methods and materials for detection and quantification of Egr protein related nucleic acids.
    Type: Grant
    Filed: June 6, 1995
    Date of Patent: February 2, 1999
    Assignee: Arch Development Corporation
    Inventor: Vikas P. Sukhatme
  • Patent number: 5863717
    Abstract: Two oligonucleotides are provided which function as amplifying primers when used in tandem in the polymerase chain reaction with HPV DNA. The primers are of the formulae:IU: 5'-TIIN.sub.1 IN.sub.1 IIN.sub.2 TAAAACGAAAGT-3'andIWDO: 5'-N.sub.1 TCN.sub.1 N.sub.3 AIGCCCAN.sub.2 TGIACCAT-3'wherein N.sub.1 is A or G, N.sub.2 is C or T and N.sub.3 is A or T.
    Type: Grant
    Filed: November 3, 1989
    Date of Patent: January 26, 1999
    Assignee: Abbott Laboratories
    Inventors: Wayne D. Lancaster, Lucie Gregoire
  • Patent number: 5861287
    Abstract: Methods for the use of a class of dyes for improved DNA sequencing are provided. A new class of dyes, BODIPY.RTM. fluorophores, has been described recently. The parent heterocyclic molecule of the BODIPY.RTM. fluorophores is a dipyrrometheneboron difluoride compound which is modified to create a broad class of spectrally-discriminating fluorophores. The present invention provides methods for the use of BODIPY.RTM. fluorophore-labeled DNA for dye-primer sequencing in which the BODIPY.RTM.s are attached to the 5' end of sequencing primers. BODIPY.RTM. fluorophores have improved spectral characteristics compared to conventional fluorescein and rhodamine dyes. BODIPY.RTM. fluorophores have narrower band width, insensitivity to solvent or pH, and improved photostability, thus, BODIPY.RTM. fluorophores lead to improved DNA sequencing and/or detection in any method where electrophoresis and detection of DNA is required. Additionally, the spectral properties of the BODIPY.RTM.
    Type: Grant
    Filed: October 6, 1995
    Date of Patent: January 19, 1999
    Assignee: Baylor College of Medicine
    Inventors: Michael L. Metzker, Richard A. Gibbs
  • Patent number: 5861124
    Abstract: An apparatus for detecting denaturation of a nucleic acid, including: denaturation condition controlling means for controlling condition of denaturation under which a double-stranded nucleic acid is separated into a first single-stranded nucleic acid and a second single-stranded nucleic acid; excitation light irradiation means for irradiating the double-stranded nucleic acid before denaturation, and the first single-stranded nucleic acid and second single-stranded nucleic acid after the denaturation; fluorescence detection means for detecting fluorescence emission based on the excitation light irradiation; and processing means for receiving, storing and processing a signal supplied from the fluorescence detection means.
    Type: Grant
    Filed: August 29, 1994
    Date of Patent: January 19, 1999
    Assignee: Hamamatsu Photonics KK
    Inventors: Shigeru Hosoi, Tadashi Fukami, Akihiko Tsuji, Makiko Hiyoshi
  • Patent number: 5858987
    Abstract: The present invention relates to the discovery that antisense nucleic acids complimentary to an E6AP gene can be used to regulate cellular p53 levels. In general the invention features E6AP antisense constructs which, by inhibiting E6AP activity, can modulate cellular p53 levels in both p53+ transformed cells and in normal cells. The invention also provides methods for treating papillomavirus (PV) induced condition, methods for regulating cellular p53 levels and methods for regulating cellular proliferation.
    Type: Grant
    Filed: May 5, 1995
    Date of Patent: January 12, 1999
    Assignee: Mitotix, Inc.
    Inventors: Peggy L. Beer-Romero, Giulio Draetta, Mark Rolfe
  • Patent number: 5856096
    Abstract: Improved telomerase activity assays are provided in which a ligation sequential reaction (LSR) or BrdUTP are used to identify a telomerase specific product. These assays are useful in diagnosing various cancers and determining the clinical prospects for cancer patients. In addition, the assays can be used to screen for substances that interfere with telomerase activity.
    Type: Grant
    Filed: September 21, 1995
    Date of Patent: January 5, 1999
    Assignee: CTRC Research Foundation
    Inventors: Bradford E. Windle, Ming Qiu, Shih-Fong Chen, Terace M. Fletcher, Ira Maine
  • Patent number: 5856442
    Abstract: Transcripts of the major histocompatibility complex (MHC) Class I HLA-G gene which are present in foetal trophoblasts and/or in adult circulating mono-nuclear cells, as well as methods of using these. Either the said transcripts comprise, in succession in the 5' to 3' direction: a fragment encoding the signal peptide (exon 1), a fragment encoding the .alpha.1 domain (exon 2), a fragment encoding the .alpha.2 domain (exon 3), a fragment encoding the transmembrane TM domain (exon 5), a fragment encoding the cytoplasmic domain (exon 6) and the 3' untranslated fragment (exon 8), which sequence is designated HLA-G4, or the said transcripts comprise intron 4.
    Type: Grant
    Filed: March 17, 1995
    Date of Patent: January 5, 1999
    Assignee: Commissariat a l'Energie Atomique
    Inventors: Edgardo Delfino Carosella, Philippe Moreau, Eliane Gluckman, Marek Kirszenbaum
  • Patent number: 5856089
    Abstract: The present invention is directed to in situ hybridization methods using nucleic acid probes for single copy sequences for detecting chromosomal structural abnormalities in fixed tissue obtained from a patient suspected of having a chromosomal structural abnormality.
    Type: Grant
    Filed: July 22, 1994
    Date of Patent: January 5, 1999
    Assignee: Oncor, Inc.
    Inventors: Mary Ge Wang, Albert Louis George, Jr., Elizabeth Sophia Light