Abstract: Combinations, called matrices with memories, of matrix materials with remotely addressable or remotely programmable recording devices that contain at least one data storage unit are provided. The matrix materials are those that are used in as supports in solid phase chemical and biochemical syntheses, immunoassays and hybridization reactions. The data storage units are preferably non-volatile antifuse memories. By virtue of this combination, molecules and biological particles, such as phage and viral particles and cells, that are in proximity or in physical contact with the matrix combination can be labeled by programming the memory with identifying information and can be identified by retrieving the stored information. Combinations of matrix materials, memories, and linked molecules and biological materials are also provided.

Abstract: Methods are provided for the determination of telomere length. These methods can be used for diagnosis of cancer and the staging of cancer, and diagnosis of senecesence in cells. Also, the instant methods can be used to determine stages of diseases such as atherosclerosis or HIV infection, and can be used to diagnose infertility.

Abstract: Discoveries are disclosed that show particular aspects of recombinant DNA technology can be used successfully to produce hitherto unknown human keratinocyte growth factor (KGF) protein free of other polypeptides. These proteins can be produced in various functional forms from spontaneously secreting cells or from DNA segments introduced into cells. These forms variously enable biochemical and functional studies of this novel protein as well as production of antibodies. Means are described for determining the level of expression of genes for the KGF protein, for example, by measuring mRNA levels in cells or by measuring antigen secreted in extracellular or body fluids.

Abstract: A thermal cycling reaction apparatus comprises a reactor having a reactor body made of a thin heat-conductive plate having a cavity as a reaction chamber with an opening of the chamber sealed with a transparent heat-resistant sheet. The apparatus further includes, delivery rollers for delivering the reactor along a delivery path and stopping it at stopping positions in a predetermined order; plural temperature-controlling blocks having respectively a temperature-controlling surface to be brought into contact with a heat-transfer face of the reactor and being placed at the stopping positions separately so as not to thermally affect each other; and a temperature-controlling mechanism for keeping the fixed temperature-controlling surfaces respectively at prescribed temperatures.

Abstract: A method for the analysis of DNA sequences and PCR products comprises the steps of constructing an oligonucleotide-labeled bead set, and labeled complementary probe, and exposing the bead set and probe to a DNA fragment or PCR product under hybridizing conditions and analyzing the combined sample/bead set by flow cytometry. Flow cytometric measurements are used to classify beads within an exposed bead set to determine the presence of identical or nonidentical sequences within the test sample. The inventive technology enables the rapid analysis of DNA sequences and detection of point mutations, deletions and/or inversions while also reducing the cost and time for performing genetic assays.

Abstract: The invention discloses a method for detecting polymorphism in the angiotensin converting enzyme by means of a chain amplification technique, the aim being to detect whether or not the sequence of nucleotides 1451-1738 of intron 16 is present in the human angiotensin converting enzyme gene.

Abstract: Methods for amplifying a nucleic acid molecule which employs a single primer, and in which the amplification is performed under isothermal conditions. The invention also includes kits containing reagents for conducting the method.

Abstract: Magnetic porous inorganic siliceous materials having a particle size of about 1 to about 200 microns useful as solid supports in various chromatography, immunoassays, synthesis and other separation and purification procedures is disclosed.

Abstract: An apparatus for placing at least one biological reagent at a plurality of locations on a substrate includes a stamp member onto which the at least one biological reagent is applied. The stamp member defines a plurality of transfer elements patterned to correspond to the plurality of locations. The stamp member contacts the substrate to transfer the at least one biological reagent from the plurality of transfer elements to the plurality of locations. The transfer elements can be defined by reservoirs or projected portions of the stamp member. A method of using said apparatus is also disclosed.

Abstract: The present invention provides substantially purified cryptdin peptides having a consensus amino acid sequence:X.sub.1 --C--X.sub.2 --C--R--X.sub.3 --C--X.sub.4 --E--X.sub.5 --C--X.sub.6 --C--C--X.sub.7whereinX.sub.1 is 3 to 9 amino acids;X.sub.2 is one amino acid, preferably Y, H or R;X.sub.3 is 2 or 3 amino acids;X.sub.4 is three amino acids;X.sub.5 is five amino acids;X.sub.6 is 6 to 10 amino acids; andX.sub.7 is 0 to 9 amino acids.The invention also provides a substantially purified mouse cryptdin having a consensus amino acid sequence:X.sub.1 --L--X.sub.2 --C--Y--C--R--X.sub.3 --C--K--X.sub.4 --E--X.sub.5 --G--T--C--X.sub.6 --C--C--X.sub.7whereinX.sub.1 is 3 or 4 amino acids, preferably LRD, LSKK (SEQ ID NO: 1) or LRG;X.sub.2 is 1 amino acid, preferably V, L or I;X.sub.3 is 3 amino acids, preferably KGH or *RG, where * is S, T, K, I or A;X.sub.4 is 2 amino acids, preferably GR, RR or RG;X.sub.5 is 3 amino acids, preferably RMN, RVR, RVF HMN or HIN;X.sub.

Abstract: Methods for the use of a class of dyes for improved DNA sequencing by the chain termination method of DNA sequencing, and internal labelling of polynucleotides by enzymatic incorporation of fluorescently-labeled ribonucleotides or deoxyribonucleotides are provided. A new class of dyes, BODIPY.RTM. fluorophores, has been described recently. The parent heterocyclic molecule of the BODIPY.RTM. fluorophores is a dipyrrometheneboron difluoride compound which is modified to create a broad class of spectrally-discriminating fluorophores. BODIPY.RTM. fluorophores have improved spectral characteristics compared to conventional fluorescein and rhodamine dyes. BODIPY.RTM. fluorophores have narrower band width, insensitivity to solvent or pH, and improved photostability, thus, BODIPY.RTM. fluorophores lead to improved DNA sequencing and/or detection in any method where electrophoresis and detection of DNA is required. Additionally, the spectral properties of the BODIPY.RTM.

Abstract: An apparatus and method for selectively attracting and inhibiting attraction of at least one predetermined molecule to a site in a molecular detection device utilizes a first electrode and a second electrode proximate to the site. The first electrode selectively generates a first electric field proximate to the site in response to a first signal applied thereto. The first electric field provides an attractive force to attract the at least one predetermined molecule toward the site. The second electrode selectively generates a second electric field proximate to the site in response to a second signal applied thereto. The second electric field selectively inhibits attraction of the at least one predetermined molecule toward the site by providing a repulsive force which dominates the attractive force provided by the first electric field.

Abstract: The human pol .delta. gene, responsible for a replication error phenotype in some colorectal tumors, can be used for diagnostic and therapeutic purposes. It can be used to demonstrate the existence of germline or somatic mutations in replication error (RER.sup.+) tumor cells.

Abstract: A method of nucleic acid hybridization in living cells is described, which is useful for detecting, quantitating and locating a specific nucleic acid in a cell or tissue, for selecting cells based on the expression or presence of a specific nucleic acid, and for monitoring the amount and location of a specific nucleic acid over time or under various inducing or inhibiting conditions.

Abstract: A method for producing a high-density, position-addressable gene array is disclosed. The method includes contacting an array of different-sequence oligonucleotides having a unique, known combinatorial sequence associated with each position in the array with a set of extended gene probe templates which are complementary to the oligonucleotides at one of the template end regions. After hybridization, the oligonucleotides in the array are extended by strand-directed polymerization to form the probe array. Also disclosed is a probe array device formed by the method.

Abstract: To diagnose non-insulin dependent diabetes mellitus or a genetically conditioned disposition thereto, examinations are made of tissue cells, especially skeletal muscle cells, from the person concerned for the presence of the human insulin receptor type B (HIR-B). This examination is preferably conducted with the aid of specific oligonucleotides as primers in a PCR amplification reaction of cDNA obtained from tissue RNA which enables a distinction to be made between HIR-A and HIR-B RNA.

Abstract: An isolated nucleic acid molecule is provided which encodes a mammalian signal mediator protein involved in regulation of cellular morphological alterations. The encoded protein comprises an amino-terminal SH3 domain, an internal domain containing several SH2 binding motifs, and a carboxy-terminal effector domain that can induce pseudohyphal budding in yeast. The invention also provides the novel signal mediator protein, and antibodies thereto. These biological molecules are useful as research tools and as diagnostic and therapeutic agents for the identification, detection and regulation of complex signaling events leading to morphological, potentially neoplastic, cellular changes.

Abstract: Methods for photocleavage of a polymer of ribonucleic acid using a texaphyrin or a texaphyrin metal complex are provided. A preferred method of use is the site-specific cleavage of a polymer of ribonucleic acid and a preferred texaphyrin is a derivatized texaphyrin having binding specificity, in particular, a texaphyrin covalently coupled to a site-directing molecule, preferably an oligonucleotide. Possible substrates for cleavage include messenger, ribosomal, transfer, small nuclear, and small cytosolic ribonucleic acids, and RNA cofactors, thereby inactivating these ribonucleic acids and providing a multifaceted approach for treating benign or malignant cancer cells, or other undesired cells or tissues.

Abstract: A method of making a pool of individual DNA molecular weight markers of defined base pair lengths that are resolved from one another and have substantially equal band intensities and that span the desired range of base pair lengths; the resultant pool of DNA molecular weight markers; the method of determining the presence of a nucleic acid or the length thereof in a specimen suspected of containing a nucleic acid utilizing such resultant pool of markers, and kits utilizing the method of making the pool and/or utilizing the markers.

Abstract: The present invention provides methods for synthesis, selection, and amplification of DNA and RNA oligonucleotides and analogs. The method for synthesizing an oligonucleotide involves: providing an effective amount of an isolated circular oligonucleotide template which comprises at least one copy of the desired oligonucleotide sequence linked to a cleavage site; providing an effective amount of an isolated oligonucleotide primer; annealing the primer to the circular template to form a primed circular template; and combining the primed circular template with an effective amount of at least two types of nucleotide triphosphates and an effective amount of a polymerase enzyme to form a nucleotide multimer complementary to the circular oligonucleotide template, wherein the nucleotide multimer comprises multiple copies of the oligonucleotide sequence joined end to end. Preferably, the nucleotide multimer is cleaved to produce oligonucleotides having well-defined ends.