Abstract: The present invention relates to a method of producing a heterologous polypeptide of interest in a host cell, wherein the method comprises expressing a fusion protein comprising the heterologous polypeptide of interest and a fluorescent fusion partner in a host cell modified to include a nucleic acid encoding a lytic protein operably linked to a promoter, wherein translation of the lytic protein is under control of an RNA thermometer. Also provided are E. coli cells which express the fusion protein and include a nucleic acid encoding a lytic protein operably linked to a promoter, wherein translation of the lytic protein regulated by an RNA thermometer.

Abstract: Methods to determine an individual's propensity or risk for a trait and applications thereof are described. Generally, systems and methods utilize genetic data acquired from an individual to determine their propensity or risk for a set of components that contribute to a trait. Propensity or risk of a trait is determined by assessing propensity or risk for each component of a set of components and the weighted contribution of each component to the trait.

Abstract: Described herein are modified MYB46 transcription factors that are more stable and less prone to degradation than corresponding unmodified MYB46 transcription factors. Expression of the modified MYB46 transcription factors within plants improves the structural strength, increases biomass, and enhances fiber strength of the plants.

Abstract: Genetically encoded calcium indicator (GECI) polypeptides and the nucleic acid molecules encoding such polypeptides are provided.

Abstract: Compositions comprising colicin immunity proteins and methods of using same for protein purification are described.

Abstract: The present invention pertains to hybrid RNase H2 proteins that include fragments of amino acid sequences from Pyrococcus abyssi (P.a.), Thermococcus kodakarensis (T.kod), and Pyrococcus furiosus organisms, as well as methods of using the same to improve mismatch discrimination and activity in a high-fidelity DNA polymerase buffer.

Abstract: This invention relates to compositions and methods for modifying Homeodomain-leucine zipper (HD-Zip) transcription factors in plants to suppress shade avoidance response in plants. The invention further relates to plants produced using the methods and compositions of the invention.

Abstract: Methods of making synthetic yeast cells by mating together two diploid (or higher ploidy) yeast species or hybrids to generate multi-ploid yeast hybrids are provided herein. The synthetic yeast cells made by this process and kits for performing the process are also provided.

Abstract: Aspects relate to systems and methods for high-contrast cleavage detection. An exemplary method includes a plurality of probes, wherein at least a probe of the plurality of probes comprises a high-contrast agent, a waveguide configured to propagate an electromagnetic (EM) wave, wherein the waveguide includes a surface upon which the plurality of probes are immobilized, wherein the surface is configured to provide communication between the EM wave and the high-contrast agent, a primary cleaving agent proximal the plurality of probes, wherein the primary cleaving agent and the plurality of probes are configured to selectively cleave the at least a probe as a result of the primary cleaving agent being in presence of an analyte, and a sensor in communication with the waveguide and configured to detect cleaving of the at least a probe by way of the EM wave.

Abstract: Therapeutic uses P2X7 inhibition and inhibition of IRAK1 and/or IRAK4, methods protecting a cell, and screening methods for identifying inhibitors are described herein.

Abstract: A construction method of a regulation system for gene expression in Zymomonas mobilis and applications are provided. The T7 expression system in Zymomonas mobilis has been constructed. The T7 expression system is used to construct a regulation circuit, help analyze the function of toxic genes and realize the regulation of metabolic pathways in Zymomonas mobilis. At the same time, shuttle plasmids that can be used in Zymomonas mobilis and Escherichia coli, also be constructed to facilitate the construction of plasmid and improve the expression efficiency of foreign genes in Zymomonas mobilis, laying a foundation for subsequent protein expression and secretion and metabolic engineering pathway optimization regulation.

Abstract: Disclosed herein are novel 5? UTR sequences that can be used to control gene expression in various contexts. Also disclosed herein are methods of engineering 5? UTR sequences and methods and kits for screening 5? UTR sequences for a property of interest.