Patents Examined by Eggerton Campbell
  • Method for determining lung adenocarcinomas by assaying for one or more of MAGE-1, MAGE-2 and MAGE-3
    Patent number: 5763165
    Abstract: A method for determining lung adenocarcinomas is described. The method involves assaying for expression of a gene coding for at least one of tumor rejection antigen precursors MAGE-1, 2 and 3, or their expression product.
    Type: Grant
    Filed: March 10, 1994
    Date of Patent: June 9, 1998
    Assignee: Ludwig Institute for Cancer Research
    Inventors: Thierry Boon-Falleur, Patrick Weynants, Bernard Lethe, Francis Brasseur, Marie Marchand, Charles DeSmet, Christophe Lurquin, Pierre van der Bruggen, Etienne DePlaen
  • Process for the production of primers and template bank therefor
    Patent number: 5763227
    Abstract: The invention relates to a process for the production of a primer for consecutive DNA sequencing, in which at least two shortmers are hybridised adjacent to one another using a template and are then ligated with one another to form the primer, the process being characterised in that(a) a template in the form of a single strand of nucleotides is used in which the number of nucleotides is at least 6 and is not greater than approximately twice the total number of nucleotides of all the shortmers used, and(b) the primer formed is then separated from the template and from unreacted shortmers and recovered. The invention relates also to banks (libraries) of templates for the mentioned process.
    Type: Grant
    Filed: April 29, 1996
    Date of Patent: June 9, 1998
    Assignee: Gesellschaft fur Biotechnologische Forschung mbH (GBF)
    Inventor: Helmut Blocker
  • Multichromophore fluorescent DNA intercalation complexes
    Patent number: 5763162
    Abstract: Novel fluorescent labeling techniques and fluorescent labels are provided, employing high affinity non-covalently binding and intercalating fluorescent dyes and dsDNA. The dyes find application to provide highly sensitive labeling of nucleic acids in electrophoretic gels and as pre-prepared labels for binding to a wide variety of specific binding pair members. The DNA-dye fluorescer complex can be used for labels in diagnostic assays, detection of specific nucleic acid sequences, and the like.
    Type: Grant
    Filed: December 2, 1993
    Date of Patent: June 9, 1998
    Assignee: The Regents of University of California
    Inventors: Alexander N. Glazer, Richard A. Mathies, Konan Peck
  • Methods for enriching target nucleic acid sequences
    Patent number: 5759780
    Abstract: The invention includes selective amplification methods for enriching target nucleic acid sequences that are present in a first source of nucleic acid but absent from a second source of nucleic acid. These methods involve using phenol to enhance the rate of the hybridization step, as well as modifications for reducing the amounts of reagents required for the reactions. Also included are methods for identifying sequences shared by two or more populations of nucleic acids.
    Type: Grant
    Filed: March 29, 1996
    Date of Patent: June 2, 1998
    Assignee: PathoGenesis Corporation
    Inventors: Jay David Parker, Kirsten Towne Smith, Peter Bruce Challoner
  • Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores
    Patent number: 5760201
    Abstract: Novel fluorescent heterodimeric DNA-staining energy transfer dyes are provided combining asymmetric cyanine azole-indolenine dyes, which provide for strong DNA affinity, large Stokes shifts and emission in the red region of the spectrum. The dyes find particular application in gel electrophoresis and for labels which may be bound to a variety of compositions in a variety of contexts. Kits and individual compounds are provided, where the kits find use for simultaneous detection of a variety of moieties, particularly using a single narrow wavelength irradiation source. The individual compounds are characterized by high donor quenching and high affinity to dsDNA as a result of optimizing the length of the linking group separating the two chromophores.
    Type: Grant
    Filed: July 12, 1995
    Date of Patent: June 2, 1998
    Assignee: The Regents of the University of California
    Inventors: Alexander N. Glazer, Scott C. Benson
  • Detection of nucleic acids in cells by thermophilic strand displacement amplification
    Patent number: 5756702
    Abstract: Thermophilic Strand Displacement Amplification (tSDA) for amplification of nucleic acid target sequences in situ in cells in suspension, on slides or in tissues is described. Excellent specimen morphology is preserved, and either DNA targets, RNA targets, or both may be selectively amplified. In situ amplification by tSDA is compatible with immunochemical techniques, so that both amplification of target sequences and immunological staining can be perfonned on the same specimen.
    Type: Grant
    Filed: January 10, 1997
    Date of Patent: May 26, 1998
    Assignee: Becton, Dickinson and Company
    Inventors: Kenton L. Lohman, Natalie V. Ostrerova, Mark Van Cleve, Robert Alan Reid
  • DNA primer and a method for screening DNAS
    Patent number: 5756295
    Abstract: DNA primers each having a nucleotide sequence substantially complementary to a nucleotide sequence which codes for Gly-Lys-Arg, Gly-Lys-Lys or Gly-Arg-Arg; methods for amplifying a polynucleotide which codes for a peptide or a precursor protein thereof using said DNA primer; screening methods for a polynucleotide which codes for a peptide comprising an amino acid sequence of Gly-Lys-Arg, Gly-Lys-Lys or Gly-Arg-Arg or a precursor protein thereof from a DNA library, etc. using said DNA; the polynucleotide obtained by said screening method; and the peptide or its precursor protein encoded by said polynucleotide, or a salt thereof. When the DNA primer of the present invention is used, the DNA which codes for a peptide having a useful physiological activity or a precursor protein thereof can be efficiently cloned.
    Type: Grant
    Filed: December 1, 1995
    Date of Patent: May 26, 1998
    Assignee: Takeda Chemical Industries, Ltd.
    Inventors: Haruo Onda, Masaki Hosoya
  • Methods for identifying fetal cells
    Patent number: 5750339
    Abstract: The invention features a method of distinguishing fetal cells from maternal cells in a sample. The method comprises the steps of contacting a sample comprising fetal cells and maternal cells with a probe which is complementary to HLA-G mRNA and identifying individual cells in which the probe hybridize to mRNA in the cells. The absence of hybridization indicates that the cells are maternal cells. The presence of hybridization indicates that the cells are fetal cells. Fetal cells identified in accordance with the invention may be tested for genetic abnormalities.
    Type: Grant
    Filed: November 30, 1994
    Date of Patent: May 12, 1998
    Assignee: Thomas Jefferson University
    Inventor: J. Bruce Smith
  • Mono-allelic mutation analysis for identifying germline mutations
    Patent number: 5750352
    Abstract: A diagnostic strategy for detection of inherited diseases caused by germline mutations is based on somatic cell hybridization. Each allele of a human gene involved in the inherited disease is isolated in a somatic cell hybrid. The products of the isolated human allele are then observed in the absence of the other allele of the human.
    Type: Grant
    Filed: August 23, 1995
    Date of Patent: May 12, 1998
    Assignee: The Johns Hopkins University
    Inventors: Bert Vogelstein, Kenneth W. Kinzler, Nickolas Papadopoulos
  • Discontinuous probe design using hybritope mapping
    Patent number: 5747248
    Abstract: This invention provides a method of detecting or determining a binding oligonucleotide comprising a nucleotide sequence which binds within a known nucleotide sequence of a target nucleic acid using a technique called hybritope mapping. This invention also provides a method of using hybritope mapping to obtain discontinuous probes that bind to a target nucleic acid.
    Type: Grant
    Filed: December 5, 1994
    Date of Patent: May 5, 1998
    Assignee: Chiron Corporation
    Inventor: Mark L. Collins
  • Homogeneous DNA probe titration assay
    Patent number: 5747256
    Abstract: An assay for detecting the presence or amount of a target polynucleotide sequence of interest in a test sample is disclosed. The assay comprises the steps of forming a reaction mixture by combining in an assay medium (i) a first reagent and (ii) an aliquot of the test sample suspected of containing the target polynucleotide sequence. The reaction mixture is subjected to denaturing conditions, and exposed to hybridization conditions allowing the first reagent to hybridize with any target polynucleotide present. A second reagent is then added, the reaction mixture undergoes hybridization conditions and the change in turbidity of the reaction mixture is detected, correlating with the presence of the target polynucleotide in the sample.
    Type: Grant
    Filed: December 19, 1995
    Date of Patent: May 5, 1998
    Assignee: Beckman Instruments, Inc.
    Inventors: Cheng F. Yan, Fredrick S. Yein
  • DNA encoding ATP-sensitive potassium channel genes
    Patent number: 5744594
    Abstract: This invention relates to DNA and protein compositions useful in the diagnosis and treatment of diabetes, heart disease and skeletal muscle disease. More specifically, this invention relates to DNA and protein compositions for ATP-sensitive potassium channel proteins, and methods of using these compositions.
    Type: Grant
    Filed: February 7, 1995
    Date of Patent: April 28, 1998
    Assignee: Oregon Health Sciences University
    Inventors: John P. Adelman, Michael J. Ashford, Chris T. Bond
  • Chimera oligonucleotide and its utilization for obtaining transcripts of a nucleic acid
    Patent number: 5744308
    Abstract: A chimera oligonucleotide is provided that can be used in a process for obtaining transcripts and/or amplification of a target sequence of a nucleic acid, having, at its 3' end, a downstream sequence. The oligonucleotide comprises successively, from 5' to 3', 1) a first oligonucleotide segment, of the DNA type, comprising a sense sequence of a promoter of an RNA polymerase, 2) a second oligonucleotide segment, of the DNA type, capable of hybridizing with the downstream sequence, and 3) a third oligonucleotide segment, of the RNA type, capable of hybridizing with a part of the target sequence contiguous to the downstream sequence, the third segment being blocked at 3'. A process using the chimera oligonucleotide and an enzyme system containing DNA polymerase activity, RNA polymerase activity, and a third activity, for example, an RNase H activity provides transcription products of the target.
    Type: Grant
    Filed: September 26, 1995
    Date of Patent: April 28, 1998
    Assignee: Bio Merieux
    Inventors: Fran.cedilla.oise Guillou-Bonnici, Philippe Cleuziat, Fran.cedilla.ois Mallet, Pierre Levasseur, William McAllister
  • Strand displacement amplification using thermophilic enzymes
    Patent number: 5744311
    Abstract: Strand Displacement Amplification methods (thermophilic SDA) which can be performed over a broad temperature range (37.degree. C. to 70.degree. C.). The preferred temperature range for thermophilic SDA is 50.degree. C. to 70.degree. C. It has been found that certain thermophilic restriction endonucleases are capable of nicking the hemimodified restriction endonuclease recognition/cleavage site as required by SDA and dissociating from the site. It has further been found that certain thermophilic polymerases are capable of extending from the nick while displacing the downstream strand. Thermophilic SDA, because of reaction temperatures higher than previously possible with conventional SDA enzyme systems, has improved specificity and efficiency, reduced nonspecific background amplification, and potentially improved yields of amplification products.
    Type: Grant
    Filed: August 22, 1996
    Date of Patent: April 28, 1998
    Assignee: Becton, Dickinson and Company
    Inventors: Melinda S. Fraiser, Catherine A. Spargo, George Terrance Walker, Mark Van Cleve, David James Wright, Michael C. Little
  • Microtiter well for detecting nucleic acid
    Patent number: 5741638
    Abstract: A microtiter well in which a single stranded nucleic acid including a plurality of sequences specifically hybridizalbe with a target nucleic acid is immobilized is disclosed. The single stranded nucleic acid is derived from a phage or a phage-plasmid. The microtiter well enables a target nucleic acid to be specifically detected with a high sensitivity and high efficiency and further enables the detection procedure to be automated.
    Type: Grant
    Filed: December 19, 1994
    Date of Patent: April 21, 1998
    Assignee: Wakunaga Seiyaku Kabushiki Kaisha
    Inventor: Akio Yamane
  • Oligonucleotide clamps
    Patent number: 5741643
    Abstract: Compounds referred to herein as oligonucleotide clamps are provided that stably bind to target polynucleotides in a sequence-specific manner. The oligonucleotide clamps comprise one or more oligonucleotide moieties capable of specifically binding to a target polynucleotide and one or more pairs of binding moieties covalently linked to the oligonucleotide moieties. In accordance with the invention, upon annealing of the oligonucleotide moieties to the target polynucleotide, the binding moieties of a pair are brought into juxtaposition so that they form a stable covalent or non-covalent linkage or complex. The interaction of the binding moieties of the one or more pairs effectively clamps the specifically annealed oligonucleotide moieties to the target polynucleotide.
    Type: Grant
    Filed: June 5, 1995
    Date of Patent: April 21, 1998
    Assignee: Lynx Therapeutics, Inc.
    Inventors: Sergei M. Gryaznov, David H. Lloyd
  • Discontinuous probe design using hybritope mapping
    Patent number: 5736327
    Abstract: This invention provides a method of detecting or determining a binding oligonucleotide comprising a nucleotide sequence which binds within a known nucleotide sequence of a target nucleic acid using a technique called hybritope mapping. This invention also provides a method of using hybritope mapping to obtain discontinuous probes that bind to a target nucleic acid.
    Type: Grant
    Filed: May 5, 1995
    Date of Patent: April 7, 1998
    Assignee: Chiron Corporation
    Inventor: Mark L. Collins
  • HBV capture and amplifiers probes for use in solution phase sandwich hybridization assays
    Patent number: 5736316
    Abstract: Novel DNA probe sequences for detection of HBV in a sample in a solution phase sandwich hybridization assay are described. Amplified nucleic acid hybridization assays using the probes are exemplified.
    Type: Grant
    Filed: January 24, 1994
    Date of Patent: April 7, 1998
    Assignee: Chiron Corporation
    Inventors: Bruce D. Irvine, Janice A. Kolberg, Joyce A. Running, Michael S. Urdea
  • Telomere repeat binding factor and diagnostic and therapeutic use thereof
    Patent number: 5733730
    Abstract: The present invention relates to a novel nucleotide sequence encoding a telomeric protein which binds a repeat region of telomeric sequences, and to the protein encoded thereby. Also included within the invention are expression vectors for the production of the telomeric protein and host cells transformed with the nucleotide sequence. In addition, antibodies, probes and antagonists specific for the telomeric protein are contemplated. Methods of identifying antagonists of the telomeric protein, diagnostic methods of identifying the telomeric protein in a sample, and therapeutic uses of the telomeric protein, particularly in the treatment of aging and cancer, are also contemplated.
    Type: Grant
    Filed: August 25, 1995
    Date of Patent: March 31, 1998
    Assignee: The Rockefeller University
    Inventor: Titia De Lange
  • Diagnostic compositions, elements, methods and test kits for amplification and detection of two or more DNA's using primers having matched melting temperatures
    Patent number: 5733751
    Abstract: An aqueous composition containing primers for opposing strands of two or more target nucleic acids can be used in polymerase chain reaction to provide simultaneously rapid and efficient amplification and detection of those nucleic acids. The primers for each target DNA differ in length by no more than 5 nucleotides and have a T.sub.m within the range of from about 65.degree. to about 74.degree. C., while the T.sub.m 's are within about 5.degree. C. of each other. Such compositions are useful in diagnostic test kits and methods for amplification and detection of multiple nucleic acids, or in "multiplexing", using multiple capture probes. All of the capture probes have T.sub.m 's which are greater than 50.degree. C. and are within 15.degree. C. of each other.
    Type: Grant
    Filed: June 8, 1995
    Date of Patent: March 31, 1998
    Assignee: Johnson & Johnson Clinical Diagonstics, Inc.
    Inventors: Thomas J. Cummins, Susan Melissa Atwood, Lynn Bergmeyer, John Bruce Findlay, John W. H. Sutherland, JoAnne H. Kerschner