Abstract: A method for performing a microparticle diagnostic assay in a device having a shallow sample well for receiving a sample and reagents for forming a reaction mixture, a read well positioned adjacent to said sample well and separated from said sample well by a wall which is constructed and arranged such that when wash fluid is injected into said sample well, sample and reaction mixtures are washed from said shallow sample well, over the wall and into said read well. The steps of the method comprise forming microparticle analyte complexes in said shallow sample well; washing said microparticle analyte complexes from said shallow sample well over the wall and into said read well where a fibrous matrix retains and immobilizes said microparticle analyte complexes; adding to said read well an indicator substance capable of forming an assay signal when said microparticle analyte complexes are present; and detecting said assay signal produced by said indicator substance.

Abstract: A method for the diagnosis of IgA nephropathy using a specific binding reaction comprising the steps:a) preparing a substrate capable of binding fibronectin or IgAb) contacting the substrate resulting from step a) with a sample of body fluid drawn from a patient subject to diagnosis to bind any fibronectin-IgA-complex present in said sample to the substrate, andc) determining the presence of complex bound to the substrate using the reaction between the exposed part of such bound complex and a corresponding antibody thereto; anda diagnostic kit for use in such diagnosis.

Abstract: A process for the determination of fructosamine in body fluids by the reaction of a sample solution with a color reagent, wherein the sample liquid is mixed with a buffer solution having a pH value of from 9 to 12, a color-forming reagent and uricase, as well as with at least one detergent, and the chronological change of the extinction is measured kinetically in a temperature range of from 20.degree. to 40.degree. C. at the earliest after 5 minutes.

Abstract: An immunoassay device and a method of performing immunoassay using this immunoassay device are disclosed. The immunoassay device includes a plate substrate having a flat surface, a plurality of adjacent projections projecting on the flat surface of the plate substrate, and a plurality of immunoreaction regions formed by applying and fixing immunoassay reagents to and in flat regions defined by the adjacent projections. The immunoreaction regions are spaced apart from each other and have surface levels equal to or higher than a surface level of the plate substrate. The immunoassay device is used to simultaneously perform various types of immunoassay and requires a simple washing for immunoassay. Specimens do not flow out from the plate or mix with each other, and safe, accurate immunoassay can be performed.