Patents Examined by Ethan C. Whisenant
  • Patent number: 11993807
    Abstract: An affinity reagent, having: (a) a retaining component such as a structured nucleic acid particle; and (b) one or both of (i) one or more label components attached to the retaining component, and (ii) one or more binding components attached to the retaining component.
    Type: Grant
    Filed: March 7, 2023
    Date of Patent: May 28, 2024
    Assignee: Nautilus Subsidiary, Inc.
    Inventors: Tural Aksel, Torri Rinker, Markus Burns, Michael Dorwart, Rachel Galimidi, Dmitriy Gremyachinskiy, Stephen Hendricks, Elvis Ikwa, Gregory Kapp, Joshua Simon Klein, Julia Robinson, Cassandra Stawicki, Sonal Tonapi, Parag Mallick
  • Patent number: 11987848
    Abstract: A method of processing a fecal sample from a human subject comprising combining a first portion of a collected fecal sample with a stabilizing buffer that maintains DNA integrity in a fecal sample, and combining a second portion of the sample with a solution that prevents denaturation or degradation of blood proteins found in a fecal sample. Embodiments comprise testing DNA extracted from the first portion of the fecal sample for the presence of a human DNA, and testing the second portion of the fecal sample for the presence of human blood.
    Type: Grant
    Filed: March 7, 2023
    Date of Patent: May 21, 2024
    Assignee: Exact Sciences Corporation
    Inventor: Joost Louwagie
  • Patent number: 11981965
    Abstract: Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.
    Type: Grant
    Filed: January 23, 2023
    Date of Patent: May 14, 2024
    Assignee: 10x Genomics, Inc.
    Inventors: James Michael Chell, Marlon Stoeckius, Jonathan Alles, Caroline Julie Gallant, Christina Galonska, Felice Alessio Bava, Layla Katiraee
  • Patent number: 11976321
    Abstract: In certain aspects, the invention disclosed herein relates to the isothermal amplification of probe linkage products to generate specific amplified signals. In some aspects, the invention provides methods, reagents, and kits for carrying out such amplification via the isothermal chain reaction (ICR).
    Type: Grant
    Filed: August 26, 2020
    Date of Patent: May 7, 2024
    Inventor: Ricardo Mancebo
  • Patent number: 11970746
    Abstract: A method of processing a fecal sample from a human subject comprising combining a first portion of a collected fecal sample with a stabilizing buffer, combining a second portion of the sample with a solution that prevents denaturation or degradation of blood proteins found in a fecal sample. Embodiments comprise testing nucleic acid extracted from the first portion of the fecal sample for an amount of a human nucleic acid, and testing the second portion of the fecal sample for the presence of human blood.
    Type: Grant
    Filed: March 7, 2023
    Date of Patent: April 30, 2024
    Assignee: Exact Sciences Corporation
    Inventor: Joost Louwagie
  • Patent number: 11959125
    Abstract: Disclosed herein are methods of extracting genetic material from a diverse population of one or more types of microbes in a sample. Microbes can be prokaryotes or eukaryotes and may include bacteria, archaea, fungi, protozoa, helminths, parasites, viruses, phages, and others. Extraction may be from a single sample and subsequent identification may be through a molecular method such as qPCR, PCR, RFLP, SSCP, allele specific PCR, targeted sequencing, pull down sequencing, whole shotgun sequencing, or other methods. Also provided are methods that include extracting nucleic acid molecules from a variety of organisms such as fungi (i.e., Saccharomyces spp.), animal cells (Bos taurus), plants (e.g., Hordeum vulgare) from the gut of a human subject, performing a metagenomics analysis therefrom, and determining a probiotic treatment or dietary guidance for the subject based on the metagenomics analysis.
    Type: Grant
    Filed: April 2, 2019
    Date of Patent: April 16, 2024
    Assignee: Sun Genomics, Inc.
    Inventor: Suneer Jain
  • Patent number: 11959130
    Abstract: Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample.
    Type: Grant
    Filed: March 7, 2023
    Date of Patent: April 16, 2024
    Assignee: 10x Genomics, Inc.
    Inventors: Christina Galonska, Marco Mignardi
  • Patent number: 11946095
    Abstract: Disclosed herein are methods, compositions, and kits for barcoding nucleic acids. For example, particles for barcoding are provided, where the particle comprises: a first plurality of oligonucleotide barcodes each comprising a target-binding region, and a second plurality of oligonucleotides each comprising a target-specific target-binding region.
    Type: Grant
    Filed: December 18, 2018
    Date of Patent: April 2, 2024
    Assignee: Becton, Dickinson and Company
    Inventors: Gretchen Yinbon Lam, Eleen Shum
  • Patent number: 11926876
    Abstract: Embodiments of the present invention relate to a combination of experimental and computational workflows that allow characterization of skin and subcutaneous tissue microbial flora and its associated metabolome, aiming to first evaluate an individual's skin and subcutaneous tissue to determine if any skin condition is as a result of an imbalance or absence of commensal or mutualistic microorganisms or their associated metabolites. In particular, embodiments of the methods and the associated computational platform provided herein relate to conducting a customized or personalized test and obtaining customized or personalized information regarding the skin and subcutaneous tissue flora and its associated metabolome there from. This may be accomplished by simultaneously identifying hundreds of microorganisms or metabolites on an individual's skin and subcutaneous tissue and comparing the resulting profile to a previously compiled healthy profile from our database of skin profiles.
    Type: Grant
    Filed: March 9, 2018
    Date of Patent: March 12, 2024
    Assignee: PRODERMIQ, INC.
    Inventors: Sasan Amini, Dana Hosseini
  • Patent number: 11927591
    Abstract: The present invention provides methods of identifying neopeptides in abnormal cells, with the methods comprising sequencing long-read messenger RNA (mRNA) isolated from the abnormal cells, identifying splice variants that could be generated from the sequenced long-read mRNA, determining if the abnormal cells contain neopeptides that correlate with the identified splice variants. The methods may also comprise identifying at least one neoantigen on the neopeptides that are present in the abnormal cells.
    Type: Grant
    Filed: August 2, 2017
    Date of Patent: March 12, 2024
    Assignee: Georgetown University
    Inventor: Anton Wellstein
  • Patent number: 11891670
    Abstract: Disclosed are probes, primers, a microarray, a kit and applications for rapid detection of clinical microorganism in ophthalmology, belonging to that technical field of clinical microorganism detection. The probes of that disclosure comprise probes for respectively detect Staphylococcus epidermidis, Staphylococcus aureus, Staphylococcus haemolyticus, Pseudomonas aeruginosa, Staphylococcus hominis, Serratia marcescens, Escherichia coli, Bacillus subtilis and Enterobacter cloacae. The disclosure also discloses primers for amplifying the target bacteria, which comprises primers which can amplify gene sequence fragments with intra-species homology of more than 95 percent (%) and inter-species homology of less than 75%. The disclosure also provides a method for synthesizing the hybridization probe on the microarray, a method for hybridization reaction and a method for scanning detection.
    Type: Grant
    Filed: September 6, 2022
    Date of Patent: February 6, 2024
    Assignee: AFFILIATED EYE INSTITUTE OF SHANDONG FIRST MEDICAL UNIVERSITY
    Inventors: Yusen Huang, Bining Zhang, Wenfeng Li, Qing Liu, Zhichao Ren, Huabo Chen
  • Patent number: 11891656
    Abstract: Disclosed herein, inter alia, are compositions and methods of use thereof for interrogating a cell.
    Type: Grant
    Filed: June 14, 2022
    Date of Patent: February 6, 2024
    Assignee: Singular Genomics Systems, Inc.
    Inventors: Eli N. Glezer, Hu Cang, Zhenmin Hong
  • Patent number: 11884692
    Abstract: The present invention provides, among other things, methods of purifying messenger RNA (mRNA) including the steps of (a) precipitating mRNA from an impure preparation; (b) subjecting the impure preparation comprising precipitated mRNA to a purification process involving membrane filtration such that the precipitated mRNA is captured by a membrane; and (c) eluting the captured precipitated mRNA from the membrane by re-solubilizing the mRNA, thereby resulting in a purified mRNA solution. In some embodiments, a purification process involving membrane filtration suitable for the present invention is tangential flow filtration.
    Type: Grant
    Filed: July 6, 2022
    Date of Patent: January 30, 2024
    Assignee: TRANSLATE BIO, INC.
    Inventors: Frank DeRosa, Anusha Dias, Michael Heartlein, Shrirang Karve
  • Patent number: 11873318
    Abstract: Disclosed is a composition comprising dichloroacetic acid, a process for preparing the same and a use thereof. It has been discovered that the novel impurity is glyoxylic acid, and glyoxylic acid in dichloroacetic acid can be detected and its concentration accurately measured, by ion chromatography method.
    Type: Grant
    Filed: July 21, 2022
    Date of Patent: January 16, 2024
    Assignees: CHANGZHOU SYNTHEALL PHARMACEUTICALS CO., LTD., SHANGHAI STA PHARMACEUTICAL R&D CO., LTD.
    Inventors: Hu Cheng, Chenchen Hu, Jiaxin Song, Yang Liu, Tingting Qian, Xianzhe Wang, Jimin Yang
  • Patent number: 11859242
    Abstract: Described herein are genetic recognition reagents comprising terminal aromatic moieties that bind specifically to a template nucleic acid and concatenate. Also provided are methods of using the genetic recognition reagents, e.g., to treat or diagnose a repeat expansion disorder, such as DMI.
    Type: Grant
    Filed: December 21, 2018
    Date of Patent: January 2, 2024
    Assignee: Carnegie Mellon University
    Inventors: Danith H. Ly, Wei-Che Hsieh, Raman Bahal
  • Patent number: 11845991
    Abstract: A method of processing a fecal sample from a human subject comprising removing a portion of a collected fecal sample and adding the removed portion of the sample to a buffer that prevents denaturation or degradation of blood proteins found in the sample, and detecting the presence of human blood in the removed portion of the fecal sample. The method further comprises stabilizing the remaining portion of the fecal sample.
    Type: Grant
    Filed: June 27, 2017
    Date of Patent: December 19, 2023
    Assignee: Exact Sciences Corporation
    Inventor: Joost Louwagie
  • Patent number: 11820977
    Abstract: The present invention provides, among other things, methods of purifying messenger RNA (mRNA) including the steps of subjecting an impure preparation comprising in vitro synthesized mRNA to a denaturing condition, and purifying the mRNA from the impure preparation from step (a) by tangential flow filtration, wherein the mRNA purified from step (b) is substantially free of prematurely aborted RNA sequences and/or enzyme reagents used in in vitro synthesis.
    Type: Grant
    Filed: June 8, 2022
    Date of Patent: November 21, 2023
    Assignee: TRANSLATE BIO, INC.
    Inventors: Frank DeRosa, Anusha Dias, Michael Heartlein, Shrirang Karve
  • Patent number: 11808675
    Abstract: Reagents and methods for extracting and stabilizing metabolites at room temperature and methods in which metabolites and one or more of proteins, lipids and nucleic acids are extracted from a single sample in a unified workflow.
    Type: Grant
    Filed: May 14, 2020
    Date of Patent: November 7, 2023
    Assignee: AGILENT TECHNOLOGIES, INC.
    Inventors: Genevieve Van de Bittner, James Alexander Apffel, Steven M. Fischer, Christine A. Miller, Kristin Briana Bernick
  • Patent number: 11795499
    Abstract: The invention provides methods and compositions for separately denaturing a probe and target in hybridization applications. The invention may, for example, eliminate the use of, or reduce the dependence on formamide in hybridization applications. Compositions for use in the invention include an aqueous composition comprising at least one polar aprotic solvent in an amount effective to denature double-stranded nucleotide sequences.
    Type: Grant
    Filed: January 4, 2019
    Date of Patent: October 24, 2023
    Assignee: Agilent Technologies, Inc.
    Inventor: Steen Hauge Matthiesen
  • Patent number: 11795496
    Abstract: A method of determining responsiveness to therapy for rheumatoid arthritis.
    Type: Grant
    Filed: June 24, 2016
    Date of Patent: October 24, 2023
    Assignee: Oxford Biodynamics PLC
    Inventors: Aroul Ramadass, Ewan Hunter, Alexandre Akoulitchev