Patents Examined by Ethan Whisnant
  • Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
    Patent number: 6323030
    Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
    Type: Grant
    Filed: January 29, 1999
    Date of Patent: November 27, 2001
    Assignee: Maxygen, Inc.
    Inventor: Willem P. C. Stemmer