Patents Examined by Evelyn Y Pyla
  • Patent number: 12037569
    Abstract: This disclosure describes methods for growing cells in a controlled environment in a growing room, transporting the cells to a harvest room, and removing the cells from the controlled environment in the harvest room. The disclosed method can limit exposure of cells to contaminants by maintaining a controlled environment through cell growth and additional processing. For instance, the cells may be washed and cooled within the controlled environment. The cells are transferred to a harvest room where they are harvested and removed from the controlled environment.
    Type: Grant
    Filed: December 22, 2023
    Date of Patent: July 16, 2024
    Assignee: Upside Foods, Inc.
    Inventors: Matthew Leung, Konrad Müller-Auffermann
  • Patent number: 12024720
    Abstract: A cell cultivation method includes cultivating dedifferentiated kidney cells in a state of being non-adherent to a culture vessel for a period of 5 days or longer, forming aggregates of the kidney cells during the cultivation period, then cultivating the kidney cells in a state of having formed aggregates, during a portion of the period, and thereby restoring the physiological functions of the kidney cells.
    Type: Grant
    Filed: October 4, 2019
    Date of Patent: July 2, 2024
    Assignee: NIKKISO CO., LTD.
    Inventor: Fumihiko Kitagawa
  • Patent number: 11957719
    Abstract: The present invention relates to substantially homogenous populations of human retinal progenitor cells having the following positive surface markers: SSEA4, CD73, PTK7 and PSA-NCAM. The invention also relates to method for preparing such substantially homogeneous cell populations from human tissue using cell sorting techniques.
    Type: Grant
    Filed: March 19, 2020
    Date of Patent: April 16, 2024
    Assignee: The Schepens Eye Research Institute
    Inventors: Michael J. Young, Petr Y. Baranov
  • Patent number: 11944667
    Abstract: The present invention provides a method of making a wound treatment composition, wherein the method comprises; i) fractionating a whole blood sample into multiple samples including a platelet rich plasma (PRP) sample, a platelet poor plasma (PPP) sample and a erythrocyte sample, wherein the PRP sample has a haematocrit level of 1-10%, ii) processing a portion of the PPP and/or PRP sample to facilitate cleavage of autologous pro-thrombin present in the PPP and/or PRP to produce autologous thrombin, and iii) combining the PRP sample with a portion of the PPP sample and a portion of the thrombin produced in step (ii) to produce the wound treatment composition; wherein step ii) is performed at less than 15° C. In preferred embodiments the PRP has a haematocrit level of 2 or 8%. Wound treatment compositions produced by the methods are also provided as are compositions for use in treating chronic and acute wounds.
    Type: Grant
    Filed: March 13, 2020
    Date of Patent: April 2, 2024
    Assignee: Biotherapy Services Limited
    Inventor: Janet Hadfield
  • Patent number: 11932874
    Abstract: The present invention relates to a method of culturing mesenchymal stem cells using gamma-irradiated serum, and more particularly to a method for culturing mesenchymal stem cells, which can improve the adhesion and proliferation rate of stem cells using a medium containing gamma-irradiated serum and an antioxidant. The method for culturing mesenchymal stem cells according to the present invention can restore the adhesion and proliferation rate of stem cells when culturing the stem cells using gamma-irradiated FBS, which is safe from contamination sources but reduces the efficiency of adhesion and proliferation of the cells. Thus, the inventive method is useful for the production of stem cells for cell therapy.
    Type: Grant
    Filed: May 24, 2019
    Date of Patent: March 19, 2024
    Assignees: R BIO CO., LTD., NATURECELL CO., LTD.
    Inventors: Jeong Chan Ra, Sung Keun Kang, Eun-Young Kim
  • Patent number: 11912924
    Abstract: The present invention provides a composition for antifreezing including a gold (Au) nanostructure in which at least a portion thereof is concave, thereby it is possible to increase a survival rate of cells due to having excellent effect of inhibiting ice recrystallization when cryopreservation of the cells, and maintain a texture of food even when using in the freezing of food.
    Type: Grant
    Filed: December 3, 2019
    Date of Patent: February 27, 2024
    Assignee: Korea University Research and Business Foundation
    Inventors: Dong June Ahn, Sang Yup Lee, Yong Ho Cho
  • Patent number: 11903974
    Abstract: Therapeutic chondrisome compositions and related methods are described.
    Type: Grant
    Filed: November 30, 2016
    Date of Patent: February 20, 2024
    Assignees: FLAGSHIP PIONEERING INNOVATIONS V, INC., The Children's Medical Center Corporation
    Inventors: Geoffrey A. von Maltzahn, John Miles Milwid, Michael Travis Mee, Jacob Rosenblum Rubens, David Chess, Kyle Marvin Trudeau, Kiana Mahdaviani, Jacob Feala, James D. McCully, Douglas B. Cowan
  • Patent number: 11903975
    Abstract: Therapeutic chondrisome and mitoplast compositions and related methods are described.
    Type: Grant
    Filed: November 30, 2016
    Date of Patent: February 20, 2024
    Assignees: FLAGSHIP PIONEERING INNOVATIONS V, INC., The Children's Medical Center Corporation
    Inventors: Geoffrey A. von Maltzahn, John Miles Milwid, Michael Travis Mee, Jacob Rosenblum Rubens, David Chess, Kyle Marvin Trudeau, Kiana Mahdaviani, Jacob Feala, James D. McCully, Douglas B. Cowan
  • Patent number: 11898167
    Abstract: The invention relates to devices, methods, kits, and compositions for in vitro generation of three-dimensional micro-tissues that are accurate models of heart, skeletal muscle, neuronal, and other tissues.
    Type: Grant
    Filed: October 26, 2020
    Date of Patent: February 13, 2024
    Assignees: The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Gladstone, The Regents of the University of California
    Inventors: Nathaniel Huebsch, Bruce Conklin, Kevin E. Healy, Peter Loskill
  • Patent number: 11898127
    Abstract: This disclosure describes methods for growing cells in a controlled environment in a growing room, transporting the cells to a harvest room, and removing the cells from the controlled environment in the harvest room. The disclosed method can limit exposure of cells to contaminants by maintaining a controlled environment through cell growth and additional processing. For instance, the cells may be washed and cooled within the controlled environment. The cells are transferred to a harvest room where they are harvested and removed from the controlled environment.
    Type: Grant
    Filed: September 26, 2022
    Date of Patent: February 13, 2024
    Assignee: Upside Foods, Inc.
    Inventors: Matthew Leung, Konrad Müller-Auffermann
  • Patent number: 11873508
    Abstract: There is provided a polymeric microsphere comprising a thermally responsive monomer crosslinked with a functional group monomer, wherein the functional group monomer comprises at least one of a carboxylic acid functional group or an amine functional group. The thermally responsive monomer is preferably N-isopropylacrylamide (NIPAM), and the microspheres preferably comprise a coating of polymerized catecholamines (e.g. DOPA). There is also provided a method of preparing the polymeric microsphere and uses of the polymeric microsphere in culturing, harvesting, or expanding stem cells or stromal cells. Preferably, the cells, e.g. hMSCs (human mesenchymal stem/stromal cells), are expanded or harvested in serum-free and xeno-free medium.
    Type: Grant
    Filed: January 22, 2018
    Date of Patent: January 16, 2024
    Assignee: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
    Inventors: Jackie Y. Ying, Leng Leng Chng, Daniele Zink, Aysha Farwin
  • Patent number: 11873513
    Abstract: The invention relates to progenitor cells of mesodermal lineage and their use in therapy.
    Type: Grant
    Filed: October 7, 2020
    Date of Patent: January 16, 2024
    Inventors: Thomas Averell Houze, Martin John Evans, Ajan Trevor Reginald, Ina Laura Pieper
  • Patent number: 11833270
    Abstract: A biological product for clinical xenotransplantation into a human and a method of preparing biological product for clinical xenotransplantation into a human involving producing a non-wild type, biologically engineered swine having a biologically engineered genome such that the swine does not express one or more extracellular surface glycan epitopes, is free of certain pathogens, is reared according to a bioburden-reducing procedure in a closed designated pathogen free herd, wherein the biological product is harvested following the swine being euthanized and the product is aseptically removed from the swine, the biological product is processed involving sterilization and storing the product in a sterile container, and the product does not contain one or more extracellular surface glycans, is free of certain designated pathogens, is biologically active and comprises live cells and tissues capable of vascularizing after xenotransplantation.
    Type: Grant
    Filed: June 14, 2021
    Date of Patent: December 5, 2023
    Assignee: XENOTHERAPEUTICS, INC.
    Inventors: Paul W. Holzer, Jon Adkins, Rodney L. Monroy, Elizabeth J. Chang
  • Patent number: 11795430
    Abstract: Disclosed herein is a material that may be useful as a coating for optical slides and medical implants. The material may aid or restrict grown of cells on a coating of the composite material. As such, there is provided a composite material having a substrate on the surface of which a coating layer of an amorphous metal oxide is formed. The metal oxide may be one or more of Ag2O, ZnO, ZrO2, TiO2, CuO, and Y2O3 and the coating layer may be from 5 to 100 nm thick and have a root mean square roughness of the coating surface is from 0.1 to 0.7 nm.
    Type: Grant
    Filed: November 10, 2017
    Date of Patent: October 24, 2023
    Assignees: National University of Singapore, Agency for Science, Technology, and Research
    Inventors: Michal Marcin Dykas, Kingshuk Poddar, Xuefeng Hu, Ee Jen Wilson Wang, Thirumalai Venky Venkatesan, Abhijeet Patra, Viknish Krishnan Kutty, Paul Lorenz Bigliardi, Mei Bigliardi
  • Patent number: 11739295
    Abstract: The invention discloses the method of differentiating human induced Pluripotent Stem cells (iPS cells) to obtain pigmented cells which produce melanin. The protocol described is much more efficient than any other method used to obtain melanin in vitro.
    Type: Grant
    Filed: September 21, 2018
    Date of Patent: August 29, 2023
    Assignee: UNIWERSYTET JAGIELLONSKI
    Inventors: Marcin Majka, Maciej Sułkowski
  • Patent number: 11739291
    Abstract: The present disclosure provides solid collagen constructs and tissue compositions, wherein a polymerizable collagen solution or suspension is extruded in the presence or absence of cells to formed an aligned architecture comprising solid collagen constructs such as those made with fibrillar collagen. Methods of using and of manufacturing solid collagen constructs and tissue compositions, where the component collagen is solid fibrillar collagen and cells are preferentially aligned, are also provided.
    Type: Grant
    Filed: April 25, 2018
    Date of Patent: August 29, 2023
    Assignee: Purdue Research Foundation
    Inventors: Sherry L. Voytik-Harbin, Stacey L. Halum, Sarah Brookes, Hongji Zhang
  • Patent number: 11737451
    Abstract: A system and method are provided for wet storage of tissue. In an embodiment, a solution for the wet preservation of tissue may include between about 0.1% to about 50% by volume dimethyl sulfoxide (DMSO) and one or more soluble monovalent or divalent metal cationic salts. A wet-preserved tissue and method for preparing the wet-preserved tissue for ultimate use, is also provided.
    Type: Grant
    Filed: June 10, 2020
    Date of Patent: August 29, 2023
    Assignee: Axogen Corporation
    Inventor: Mindy E. Sadik
  • Patent number: 11713446
    Abstract: The present invention provides improved and/or shortened processes and methods for reprogramming TILs in order to prepare therapeutic populations of TILs with increased therapeutic efficacy. Such reprogrammed TILs find use in therapeutic treatment regimens.
    Type: Grant
    Filed: May 4, 2022
    Date of Patent: August 1, 2023
    Assignee: IOVANCE BIOTHERAPEUTICS, INC.
    Inventors: Cecile Chartier-Courtaud, Krit Ritthipichai
  • Patent number: 11713445
    Abstract: The present disclosure provides a method of fabricating curvature-defined (C-D) or shape-defined (S-D) concave and convex polydimethylsiloxane (PDMS) surfaces and a method of fabricating C-D or S-D convex and concave gel surfaces for use in cell and tissue culturing and in other surface and interface applications, and provides a method of using C-D or S-D convex and concave surfaces with varying curvatures to direct cell attachment, spreading, and migration.
    Type: Grant
    Filed: December 13, 2019
    Date of Patent: August 1, 2023
    Inventor: Shengyuan Yang
  • Patent number: 11702634
    Abstract: Embodiments described herein generally provide for expanding cells in a cell expansion system. The cells may be grown in a bioreactor, and the cells may be activated by an activator (e.g., a soluble activator complex). Nutrient and gas exchange capabilities of a closed, automated cell expansion system may allow cells to be seeded at reduced cell seeding densities, for example. Parameters of the cell growth environment may be manipulated to load the cells into a particular position in the bioreactor for the efficient exchange of nutrients and gases. System parameters may be adjusted to shear any cell colonies that may form during the expansion phase. Metabolic concentrations may be controlled to improve cell growth and viability. Cell residence in the bioreactor may be controlled. In embodiments, the cells may include T cells. In further embodiments, the cells may include T cell subpopulations, including regulatory T cells (Tregs), for example.
    Type: Grant
    Filed: April 2, 2018
    Date of Patent: July 18, 2023
    Assignee: Terumo BCT, Inc.
    Inventors: Brian J. Nankervis, Domicinda M. Hill, Mark E. Jones