Patents Examined by Evelyn Y Pyla
  • Patent number: 11660318
    Abstract: Provided herein are novel methods and composition utilizing adipose tissue-derived stromal stem cells for treating fistulae.
    Type: Grant
    Filed: February 1, 2019
    Date of Patent: May 30, 2023
    Assignee: Takeda Pharmaceutical Company Limited
    Inventors: María Gema Fernández Miguel, Manuel Ángel González De La Pena, Rosa Ana García Castro, Mariano García Arranz, Damián García Olmo
  • Patent number: 11655451
    Abstract: The present disclosure relates to methods for culturing human epidermal keratinocytes. When keratinocytes are cultured on plates coated with a laminin containing an alpha-4 chain or an alpha-5 chain, in a xeno-free, chemically defined cell culture medium, they expand efficiently in vitro. Useful cell culture kits for culturing keratinocytes are also described herein, as are methods of using such cells for treatment of burns or chronic wounds.
    Type: Grant
    Filed: May 4, 2018
    Date of Patent: May 23, 2023
    Inventors: Karl Tryggvason, Monica Suryana Tjin, Alvin Wen Choong Chua
  • Patent number: 11649274
    Abstract: An object of the invention is to provide a method for producing a mesenchymal stem cell in which differentiation into an adipocyte is suppressed with a simple step, a mesenchymal stem cell, a method for producing a differentiation-induced cell using the method for producing a mesenchymal stem cell described above, a differentiation-induced cell, and an inhibitor of differentiation into an adipocyte. According to the invention, there is provided a method for producing a mesenchymal stem cell, including: a step of culturing a mesenchymal stem cell in a liquid medium in which a recombinant gelatin having an amino acid sequence derived from a partial amino acid sequence of collagen has been dissolved, wherein differentiation into an adipocyte is suppressed.
    Type: Grant
    Filed: November 8, 2019
    Date of Patent: May 16, 2023
    Assignee: FUJTFITM Corporation
    Inventor: Koji Muraya
  • Patent number: 11649439
    Abstract: A biological composition has a mixture of mechanically selected allogeneic biologic material derived from bone marrow. The mixture has non-whole cellular components including vesicular components and active and inactive components of biological activity, cell fragments, cellular excretions, cellular derivatives, and extracellular components. The mixture is compatible with biologic function.
    Type: Grant
    Filed: October 15, 2020
    Date of Patent: May 16, 2023
    Assignee: Vivex Biologies Group, Inc.
    Inventors: Timothy Ganey, Wendy W. Weston, Miguel Quevedo, Stuart Oglesby, Gaetan Jean-Robert Delcroix, Paul C. Schiller, Gianluca D'Ippolito
  • Patent number: 11633523
    Abstract: Provided are a method for producing a cell tissue, including a culturing step of culturing cells capable of serving as a feeder inside opening pores and communicating pores of a porous film having a plurality of the opening pores provided on a surface thereof and the communicating pores communicating mutually adjacent opening pores with one another; and a porous film including a plurality of opening pores provided on a surface thereof and communicating pores communicating mutually adjacent opening pores with one another.
    Type: Grant
    Filed: March 7, 2019
    Date of Patent: April 25, 2023
    Inventors: Koju Ito, Shun Goto, Souichi Kohashi, Kohji Nakazawa, Hiroshi Yabu
  • Patent number: 11629331
    Abstract: Provided is a medium composition for differentiation of a human induced pluripotent stem cell to a dermal papilla precursor cell, a differentiation method, and a use for inducing hair follicle neogenesis using the differentiated dermal papilla precursor cell. Further provided is a method for differentiating a human induced pluripotent stem cell into a dermal papilla precursor cell having hair follicle forming ability and a composition of a dermal papilla precursor cell specific differentiation medium for the above differentiation, and have effectively induced hair follicle neogenesis consisting only of human cells without conventional mouse-human hybrid hair follicles by using the human induced dermal papilla precursor cell and a human induced epidermal precursor cell obtained through the differentiation method. Human hair follicle tissue produced is expected to be useful as a therapeutic method for patients suffering from hair loss by overcoming the limitations of hair loss treatments.
    Type: Grant
    Filed: July 18, 2018
    Date of Patent: April 18, 2023
    Inventors: Ohsang Kwon, Bo Mi Kang, Jin Yong Kim, Yoo-Wook Kwon
  • Patent number: 11624055
    Abstract: The present disclosure relates to a method of generating bioartificial reptile leather by in vitro culturing of isolated cells the chorioallantois of hatched reptile eggs. The disclosure allows production of reptile leather without the ethical issues of conventional reptile farming and the trapping and killing of reptiles for their skin. Furthermore the disclosure allows production of reptile leather from species that are not abundantly available as skin products, such as from endangered species.
    Type: Grant
    Filed: April 5, 2018
    Date of Patent: April 11, 2023
    Inventor: Bettina C. Overgaard
  • Patent number: 11624046
    Abstract: Embodiments described herein generally provide for expanding cells in a cell expansion system. The cells may be grown in a bioreactor, and the cells may be activated by an activator (e.g., a soluble activator complex). Nutrient and gas exchange capabilities of a closed, automated cell expansion system may allow cells to be seeded at reduced cell seeding densities, for example. Parameters of the cell growth environment may be manipulated to load the cells into a particular position in the bioreactor for the efficient exchange of nutrients and gases. System parameters may be adjusted to shear any cell colonies that may form during the expansion phase. Metabolic concentrations may be controlled to improve cell growth and viability. Cell residence in the bioreactor may be controlled. In embodiments, the cells may include T cells. In further embodiments, the cells may include T cell subpopulations, including regulatory T cells (Tregs), helper, naïve, memory, or effector, for example.
    Type: Grant
    Filed: August 24, 2018
    Date of Patent: April 11, 2023
    Assignee: Terumo BCT, Inc.
    Inventors: Brian J. Nankervis, Mark E. Jones, Domicinda M. Hill
  • Patent number: 11596714
    Abstract: Provided herein are constructs of micro-aggregate multicellular, minimally polarized grafts containing Leucine-rich repeat-containing G-protein coupled Receptor (LGR) expressing cells for wound therapy applications, tissue engineering, cell therapy applications, regenerative medicine applications, medical/therapeutic applications, tissue healing applications, immune therapy applications, and tissue transplant therapy applications which preferably are associated with a delivery vector/substrate/support/scaffold for direct application.
    Type: Grant
    Filed: April 19, 2022
    Date of Patent: March 7, 2023
    Assignee: POLARITYTE, INC.
    Inventor: Denver M. Lough
  • Patent number: 11591572
    Abstract: The invention relates to differentiation methods for progenitor cells, e.g. mammalian epithelial stem cells, differentiation media for use in said methods, organoids and cells obtainable by said methods and uses, including therapeutic uses, thereof.
    Type: Grant
    Filed: March 1, 2017
    Date of Patent: February 28, 2023
    Assignee: Koninklijke Nederlandse Akademie Van Wetenschappen
    Inventors: Johannes Carolus Clevers, Helmuth Gehart
  • Patent number: 11584912
    Abstract: The present invention provides automated devices for use in supporting various cell therapies and tissue engineering methods. The present invention provides an automated cell separation apparatus capable of separating cells from a tissue sample for use in cell therapies and/or tissue engineering. The cell separation apparatus can be used in combination with complementary devices such as cell collection device and/or a sodding apparatus to support various therapies. The automated apparatus includes media and tissue dissociating chemical reservoirs, filters, a cell separator and a perfusion flow loop through a graft chamber which supports a graft substrate or other endovascular device. The present invention further provides methods for using the tissue grafts and cell samples prepared by the devices described herein in a multitude of therapies including revascularization, regeneration and reconstruction of tissues and organs, as well as treatment and prevention of diseases.
    Type: Grant
    Filed: September 28, 2015
    Date of Patent: February 21, 2023
    Inventors: Gregory D. Ariff, Thomas Cannon, Jennifer L. Case, Christian L. Haller, Paul Kosnik, Charles P. Luddy, Craig A. Mauch, Erik Vossman, Stuart K. Williams
  • Patent number: 11577000
    Abstract: The present invention relates to an in vitro method for creating a viable connective tissue and/or osseous tissue obtained by tribological solicitations of a biological culture. It further relates to a viable connective tissue and/or osseous tissue susceptible to be obtained by said method as well as to the use of said method or viable connective tissue and/or osseous tissue to prepare a biological implant.
    Type: Grant
    Filed: December 17, 2018
    Date of Patent: February 14, 2023
    Assignee: TORNIER
    Inventors: Michel Hassler, Ghassene Ouenzerfi
  • Patent number: 11564388
    Abstract: The present invention provides methods for cryopreserving a population of cells with improved cell viability. In some aspects, the method comprises contacting a population of cells with a peptoid polymer comprising one or more polar peptoid monomers, e.g., formulated in a cryoprotectant solution, and cooling the population of cells at a temperature of from 0° C. to about ?20° C. for a time period of at least about 3 hours to produce a population of supercooled cells. The supercooling methods of the present invention provide excellent post-thaw cell survival and recovery. In certain embodiments, the population of cells is present in a tissue or an organ that is cryopreserved by performing the supercooling methods of the present invention.
    Type: Grant
    Filed: September 26, 2019
    Date of Patent: January 31, 2023
    Assignee: X-THERMA, INC.
    Inventor: Xiaoxi Wei
  • Patent number: 11549097
    Abstract: The present invention relates to a process for producing a composition comprising an aqueous medium and, disposed in the aqueous medium, a first volume of a first hydrogel, which process comprises: (i) providing a composition comprising a first hydrophobic medium and, disposed in the first hydrophobic medium, a first volume of a first hydrogel; (ii) disposing a volume of an aqueous composition comprising a hydrogel compound around the first volume of the first hydrogel; (iii) allowing the aqueous composition comprising the hydrogel compound to form a gel and thereby forming a hydrogel object, which hydrogel object comprises the first volume of the first hydrogel and a second volume of a second hydrogel, which second volume of the second hydrogel is disposed around the first volume of the first hydrogel; and (iv) transferring the hydrogel object from the first hydrophobic medium to an aqueous medium and thereby producing the composition comprising the aqueous medium and, disposed in the aqueous medium, the fir
    Type: Grant
    Filed: February 28, 2017
    Date of Patent: January 10, 2023
    Assignee: Oxford University Innovation Limited
    Inventors: Hagan Bayley, Sam Olof, Alexander D. Graham
  • Patent number: 11523606
    Abstract: A system and method are provided for wet storage of tissue. In an embodiment, a solution for the wet preservation of tissue may include between about 0.1% to about 50% by volume dimethyl sulfoxide (DMSO) and one or more soluble monovalent or divalent metal cationic salts. A wet-preserved tissue and method for preparing the wet-preserved tissue for ultimate use, is also provided.
    Type: Grant
    Filed: July 27, 2020
    Date of Patent: December 13, 2022
    Assignee: Axogen Corporation
    Inventor: Mindy E. Sadik
  • Patent number: 11512278
    Abstract: A process of growing a phototrophic biomass in a reaction zone, including a reaction mixture that is operative for effecting photosynthesis upon exposure to photosynthetically active light radiation, is provided. The reaction mixture includes phototrophic biomass that is operative for growth within the reaction zone. In one aspect, the carbon dioxide supply is modulated in response to detected process parameters. In another aspect, inputs to the reaction zone are modulated based on changes to the carbon dioxide supply. In another aspect, dilution of the carbon dioxide-comprising supply is effected. In another aspect, pressure of the carbon dioxide-comprising supply is increased. In another aspect, water is condensed from the carbon dioxide-comprising supply and recovered for re-use. In another aspect, the produced phototrophic biomass is harvested at a rate which approximates a predetermined growth rate of the phototrophic biomass.
    Type: Grant
    Filed: December 16, 2015
    Date of Patent: November 29, 2022
    Assignee: Pond Technologies Inc.
    Inventors: Jaime A. Gonzalez, Max Kolesnik, Steven C. Martin, Tony Di Pietro, Emidio Di Pietro
  • Patent number: 11505780
    Abstract: The present disclosure provides a method of fabricating curvature-defined (C-D) or shape-defined (S-D) concave and convex polydimethylsiloxane (PDMS) surfaces and a method of fabricating C-D or S-D convex and concave gel surfaces for use in cell and tissue culturing and in other surface and interface applications, and provides a method of using C-D or S-D convex and concave surfaces with varying curvatures to direct cell attachment, spreading, and migration.
    Type: Grant
    Filed: December 13, 2019
    Date of Patent: November 22, 2022
    Inventor: Shengyuan Yang
  • Patent number: 11492593
    Abstract: The present invention provides a method of purifying highly pure retinal pigment epithelial cells from a cell population obtained by induction of differentiation of pluripotent stem cells into retinal pigment epithelial cells, by a simple and easy operation in a short period. The purification method of the present invention includes a step of introducing a cell population containing retinal pigment epithelial cells obtained by differentiation induction of pluripotent stem cells on laminin or a fragment thereof on a filter, and obtaining a cell population that passed the filter.
    Type: Grant
    Filed: October 9, 2014
    Date of Patent: November 8, 2022
    Inventors: Masanori Sawada, Kiyotoshi Sekiguchi
  • Patent number: 11479752
    Abstract: Provided herein are synthetic, three-dimensional (3D) bioprinted tissue constructs comprising porcine cells and methods of producing and using the same. The synthetic 3D bioprinted tissue constructs are fabricated by bioprinting spheroids comprising porcine cells, including genetically engineered cells, on a microneedle mold and fusing the spheroids to form an engineered tissue construct. Also provided are methods of using scaffold-free 3D bioprinted tissue constructs for applications related to drug screening and toxicity screening.
    Type: Grant
    Filed: October 17, 2018
    Date of Patent: October 25, 2022
    Inventor: Burcin Ekser
  • Patent number: 11473117
    Abstract: A technology regarding the production, formulation and use of conditioned media and the factors included therein is disclosed. The conditioned media may be inoculated with animal cells, plant cells and any combination thereof. The inoculations may occur simultaneous or at different times. Cells retrieved from different areas of the animal and/or the plant may also be cultured together to form conditioned media and associated growth factors.
    Type: Grant
    Filed: July 23, 2019
    Date of Patent: October 18, 2022
    Assignee: Rinati Skin, LLC
    Inventors: Nathan Newman, Alex Rajangam, Dodanim Talavera-Adame, Harpreet Sidhu