Abstract: Conjugates between one or more repeated subunits of an antigen and a carbohydrate polymer are desired. Also described are immunogenic vaccines against disease states which contain the conjugates and methods for inducing cell-mediated immune responses. The conjugates may especially contain polymers of the carbohydrate mannose and one or more repeated subunits of human mucin.

Abstract: A live, attenuated HSV-2 virus and methods of making and using the virus are provided. The live, attenuated HSV-2 virus is constructed using recombinant techniques and can be used in a pharmaceutical composition for prophylactic treatment of HSV-2 infections and for treatment of recurrent HSV-2 related diseases and conditions. Additionally, a plasmid vector is disclosed for expressing a GST-UL56 fusion protein wherein the protein comprises the Glutathione S Transferase gene product fused to at least an immunogenic portion of the HSV-2 UL56 gene product. The GST-UL56 fusion protein can be used to produce polyclonal antisera to the HSV-2 UL56 gene product, to detect whether recombinant HSV-2 deletion mutants express UL56, and as a type-specific reagent capable of discerning HSV-1 from HSV-2.

Abstract: Crosslinking reagents for protein modification are provided, which comprise four functional, leaving groups capable of reacting with amino acid residues on the protein chains, one pair at each end of a relatively rigid chemical spacer group comprising aromatic residues joined by amide linkages. Such crosslinking reagents react with hemoglobin to effect intramolecular crosslinking of two dimeric hemoglobin units to stabilize the tetramers, by use of the respective pairs of functional groups, and to produce dimers of such tetrameric units, i.e. bis-tetrameric, intramolecularly crosslinked and stabilized hemoglobin, to the exclusion of higher molecular weight hemoglobin species.

Abstract: A nucleic acid comprising a sequence encoding a fusion protein comprising a portion of the glycoprotein B of HCMV fused to a portion of the glycoprotein D of HSV-2.

Abstract: The invention provides for compositions and methods for interfering with Hepatitis B viral infection that are based on the interaction of Hepatitis B virus X protein with a novel proteasome subunit.

Abstract: A protein crumble having fat-like properties is made by using a soy protein which is mixed with water and then chopped and blended. Specific amounts of isolate soy protein or soy protein concentrate are preferred. Any of a number of ingredients including animal derived proteins, carbohydrates, and gums may be added to produce a fat-like attribute without actually adding fat.

Abstract: A method for debittering and reducing the allergenic reactivity of protein hydrolysates is disclosed, which method includes the providing of an aqueous solution of a protein hydrolysate, feeding the solution into a bed of siloxane, and collecting a first portion of the solution exiting from the bed. The first portion contains either a non-bitter or bitter fraction. A second portion of the solution exiting from the bed can be collected which contains the opposite tasting fraction of the hydrolysate. Preferably the siloxane is selected from the group comprising octa-siloxane and octadecyl-siloxane. Preferably the hydrolysate has a molecular weight of less than 10,000, and is derived from the group of hydrolysates comprising casein, whey and soy. Additionally, the bed preferably is of a depth of between 2 to 4 times the bed diameter. A more hypoallergenic protein hydrolysate product produced in accordance with the method of the invention is also disclosed.

Abstract: A method for improving recovery after surgery or an invasive procedure is provided, including administering a hemoglobin preparation to a patient before surgery or an invasive procedure.

Abstract: A method for culturing an organic tissue comprising: attaching the tissue onto an inner side of a mesh which is placed in an incubation chamber, and culturing the tissue in the presence of a culture medium and a gas containing oxygen under a condition that the ratio of the contact time of the tissue with the culture medium to that with the gas lies in a range from 1:2.5 to 1:3.5.

Abstract: An antigen composition hepatitis E virus (HEV) infection are disclosed. The antigen composition includes peptides corresponding to carboxyl terminal end regions of the second and third open reading frames of the HEV genome.

Abstract: An anti-FHV-1 recombinant antibody efficacious for treatment, prevention and diagnosis of feline herpes virus-1 (FHV-1) and a gene fragment useful for preparing the same are provided. A cell producing a mouse monoclonal antibody having an excellent neutralizing activity against FHV-1 was constructed, and a gene fragment coding for V region of said antibody responsible for the specific binding activity against FHV-1 was obtained. Using this gene fragment and a gene fragment coding for the constant region of a feline antibody, a chimeric anti-FHV-1 recombinant antibody is obtained.

Abstract: The entire genome of the hepatitis D virus has been shown to be a circular single-stranded RNA Of 1679 bases. Several open reading frames in both the genomic and complementary strands indicate possible protein products. The products encoded in one open reading frame, ORF5, are identified as viral polypeptides p24.sup..delta. and p27.sup..delta. of which the nuclear .delta. antigens in HDV infected liver is comprised. These products, as well as others encoded in ORFs 1, 2, 6, and 7 are produced in recombinant expression systems. The ORF5 products, in particular, are useful for HDV diagnosis and vaccines.

Abstract: A fusion protein comprising a portion of a glycoprotein of HCMV fused to a portion of a glycoprotein of HSV is described.

Abstract: Antibodies are recovered and isolated from the sera of a number "n" of patients each of which have or have had the same given disease so that each of the "n" patients has, within a large number of different antibodies, some antibodies uniquely associated with the same disease of interest. The antibodies of a first patient are bound to a support surface. To carry out initial screening libraries of molecules are brought into contact with the bound antibodies of the first patient under conditions where binding will occur. Secondary screening is then carried out by extracting and labeling the antibodies of a second patient and using the labeled antibodies to probe the molecules (peptides) isolated in the initial screening. Many of the non-disease specific antibodies (of the second patient) will not bind to the molecules (peptides) of the isolated bacteriophage which bound to the antibodies of the first patient.

Abstract: In a data processing system which is for use in combination with at least one peripheral device and comprises an input-output processor connected to a first and a second bus, a main memory connected to the first bus, a peripheral controller connected to the second bus and the peripheral device, and a central processor connected to the first bus and coupled to the second bus through the input-output processor, the peripheral controller requests data transfer between the main memory and the peripheral device. For this purpose, the peripheral controller produces a translation request signal in response to a channel command which is stored in a local memory of the peripheral controller from the main memory through the input-output processor and indicates the data transfer.

Abstract: A drawbar assembly for interconnecting railcars comprises a drawbar having enlarged ends, each of which is positioned in a socket formed in a railcar sill having outer side walls. A drawbar pin is retained in the sill and passes through an aperture in the enlarged drawbar end, retaining the drawbar in the socket. Pin bearing block means are carried in the drawbar and are in abutting relation with the pin. Slack adjusting wedge means urge the pin against the bearing block means. In accordance with this invention, the slack adjusting wedge means are positioned in the sill outer side walls. Thus, the slack adjusting wedge means can be inspected and maintained without disassembling the drawbar assembly.

Abstract: A vehicle truck embodying articulated subtrucks or steering arms having a plurality of wheelsets, with steering arm interconnections establishing coordinated steering motions of the wheelsets, the truck also having elastic restraining devices for stabilizing steering and other motions of the wheelsets and still further having linkage interrelating relative lateral motions of the truck and body of the vehicle. A method and structure is provided for adapting or "retrofitting" existing truck structures in a manner to embody the steering and stabilizing characteristics.

Abstract: A gondola-type railway car has an end structure supported on a frameless radial truck. The end structure includes a center sill and a bottom closure member. Side sill members are connected to the sides of the bottom closure member. The undersurface of the bottom closure member has eight wedge pockets thereon with reinforcement attached thereto for engaging eight support pads on the truck. The pads and the wedge pockets are arranged in four longitudinally-spaced pairs of laterally spaced pads. The bottom closure member has a reinforcement structure on its upper surface for transferring the weight of the car to the truck. The reinforcement structure includes three transverse reinforcement channels above the three inner pairs of wedge pockets. A pair of longitudinally extending channel members extend from above the outer wedge pockets to engage the outermost transverse beam to tie the outer wedge pockets into the car body structure.

Abstract: An open-topped vehicle, such as a freight vehicle, is covered by a single-use covering which includes a cage having a standard length and adjustment elements for taking up the difference between the cage length and the length of the vehicle. The cage includes cover supporting ribs that are spaced apart from each other according to preset distances. The covering also includes a flexible cover that is shrinkable and self-weldable upon the application of heat thereto, and is attached to the vehicle by multi-ply end joints which are formed by welding a first portion of the cover to a second portion of the cover to form a hem with a line located inside the hem. The line is attached to the vehicle. Patches can also be placed in locations on the cover needing additional, multi-ply protection.

Abstract: A method of applying a cover to an open-topped vehicle, such as a freight vehicle, includes steps of applying a single-use covering which includes a cage having a standard length and adjustment elements for taking up the difference between the cage length and the length of the vehicle to the vehicle. The cage includes cover supporting ribs that are spaced apart from each other according to preset distances. The covering also includes a flexible cover that is shrinkable and self-weldable upon the application of heat thereto, and is attached to the vehicle by forming double-ply end joints by welding a first portion of the cover to a second portion of the cover to form a hem with a line located inside the hem. The line is attached to the vehicle. Patches can also be placed in locations on the cover needing multi-ply protection and heat welded to the cover at those locations.