Abstract: Devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore are provided. The devices and methods also determine (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.

Abstract: Disclosed is a cell analyzer comprising: a measuring device that includes a collecting section configured to collect target cells in a specimen with a filter, and is configured to measure the target cells collected by the collecting section; and a data processing device configured to analyze the target cells based on measurement data obtained by the measuring device, wherein the cell analyzer is operable in a first mode of measuring a clinical specimen collected from a subject and a second mode of measuring a quality control specimen containing particles having size capturable by the filter; and the data processing device is programmed to acquire an amount of particles collected by the collecting section based on measurement data of the quality control specimen obtained in the second mode, and output an alarm when the amount of particles meets a predetermined condition.

Abstract: A method for determining functional severity of a stenosis includes: (a) generating a simulated perfusion map from a calculated blood flow; (b) comparing the simulated perfusion map to a measured perfusion map to identify a degree of mismatch therebetween, the measured perfusion map representing perfusion in a patient; (c) modifying a parameter in a model used in calculating the blood flow when the degree of mismatch meets or exceeds a predefined threshold; (d) computing a hemodynamic quantity from the simulated perfusion map when the degree of mismatch is less than the predefined threshold, the hemodynamic quantity being indicative of the functional severity of the stenosis; and (e) displaying the hemodynamic quantity. Systems for determining functional severity of a stenosis are described.

Abstract: An embodiment of a method for solving the inverse problem of electrophysiology and determining a voltage distribution on a surface of a tissue may comprise receiving a plurality of voltages collected by a plurality of electrodes adjacent to the surface, discretizing the problem using a Finite Element Method (FEM) or a Boundary Element Method (BEM), introducing one or more regularization terms to an error minimization formulation, and solving, by a processor, the voltage distribution according to the plurality of voltages and according to the regularization terms. The regularization terms may comprise one or more of a Laplacian smoothness operator, a Tikhonov regularization matrix, a confidence matrix, and a linear operator that interpolates the plurality of electrode voltages to the tissue voltage distribution.

Abstract: A method to calculate and visualize dynamic wave front propagation of electrical signals on a geometric surface is described. Wave front locations are identified on the geometric surface between each identified pair of adjacent nodes on the geometric surface. A graphical map can be generated to represent the identified wave front locations on at least a portion of the geometric surface.

Abstract: A method for determining a pathology of a tissue sample. The method includes steps of coupling the tissue sample between two oppositely-facing ultrasonic transducers; acquiring a pulse-echo ultrasonic measurement and a through-transmission ultrasonic measurement of the tissue sample using the ultrasonic transducers; analyzing at least one of the pulse-echo ultrasonic measurement and the through-transmission ultrasonic measurement using time domain analysis; analyzing at least one of the through-transmission ultrasonic measurements and the pulse-echo ultrasonic measurements using frequency domain analysis; and determining the pathology of the tissue sample based on at least one of the time domain analysis and the frequency domain analysis.

Abstract: A method for genetic evaluation of an inbred plant includes construction of a phenotypic trait database incorporating at least two numerically representable phenotypic traits in a first plant population. Methods for selecting an inbred plant or hybrid plant based on genetic values can be obtained using a multivariate mixed model analysis of such a relationship matrix comprising at least two numerically representable phenotypic traits.

Abstract: A method for monitoring a cell culture, where the method includes measuring pulse-echo ultrasonic waveforms from the cell culture, and analyzing the pulse-echo ultrasonic waveforms to monitor the cell culture.

Abstract: A method of morphological reconstruction of biological activity in a tissue sample maps biological data resulting from analysis of tissue samples onto a 3-D morphological rendering of the biological sample. Each slice in a set of histological slices, indexed by a first index, is micro dissected into micro samples indexed by a pair of first and second indices. The indices are utilized to spatially map biological data to the 3-D rendering.

Abstract: A geometric model of an organ represents its shape as a collection of elements formed from nodes and connections among them. A first vessel network model represents a network of first vessels whose diameters are larger than or equal to a threshold. A plurality of second vessel networks each represent a network of second vessels whose diameters are smaller than the threshold. In a simulator apparatus, a first analysis unit analyzes hemodynamics in the first vessels, based on the geometric model and first vessel network model of the organ and reflecting the motion of the organ. A second analysis unit analyzes hemodynamics in the second vessel network models connected to the nodes, by using output data of the first analysis unit which indicates the hemodynamics in the first vessels at each of the nodes.

Abstract: A cosmetic regimen and/or a nutrition regimen is outputted almost instantly to a user who transmits a digital color image, preferably video, of the surface tissue, for example at the face of a human to a web site. The regimen is custom tailored to the surface tissue type of the subject. The surface tissue type is based on value ranges of multiple surface tissue bioparameters, such as surface tissue moisture, surface tissue collagen levels, surface tissue pH and surface tissue sun sensitivity. The system may track changes in the surface tissue parameters between one digital color image provided by a user at one time and a further digital color image provided later.

Abstract: A computer-implemented method is presented for recommending insulin dosage adjustments for a patient having diabetes. The method includes: identifying a plurality of bolus events from patient data; grouping bolus events having a recommended bolus dosage substantially equivalent to the amount of administered insulin into a first subset of bolus events; determining a bolus outcome for each of the bolus events in the first subset of bolus events, where the bolus outcome is expressed in relation to a target range of blood glucose values and is selected from a group including above the target range, in the target range and below the target range; determining whether one of the bolus outcomes is predominant amongst the bolus events in the first subset of bolus events; and generating a recommendation pertaining to insulin dosage for the patient in response to a determination that one of the bolus outcomes is predominant.

Abstract: Provided herein are methods, processes and apparatuses for non-invasive assessment of genetic variations.

Abstract: An embodiment of the present invention includes a method for discriminating between benign and malignant tissue lesions. The method includes the steps of using a plurality of maps of physiology and morphology parameters generated from reflectance measurements and morphology parameters generated from reflectance measurements. The method also includes calculating entropies and cross entropies of the plurality of maps, and calculating a plurality of morphology parameters. Further, the method includes assigning a weight to each entropy and a weight to a logarithm of each entropy, a weight to each cross entropy and a weight to a logarithm of each cross entropy, and a weight to each morphology parameter and a weight to a logarithm of each morphology parameter.

Abstract: A method can include storing input electrical signal data representing at least a given electrophysiological signal acquired from a patient. A non-local mean filter can be applied to the given electrophysiological signal, the non-local mean filter including a spatial filter component and an intensity filter component. The method can also include controlling parameters to establish weighting of each of the spatial filter component and the intensity filter component in response to a control input. Filtered signal data can be stored based on the applying and the controlling.

Abstract: Motility contrast imaging (MCI) is a depth-resolved holographic technique to extract cellular and subcellular motion inside tissue. The holographic basis of the measurement technique makes it highly susceptible to mechanical motion. The motility contrast application, in particular, preferably includes increased mechanical stability because the signal is based on time-varying changes caused by cellular motion, not to be confused with mechanical motion of the system. The use of the resulting spectrogram response signatures, or “fingerprint” data, of known compounds is disclosed to screen new compounds for leads as to those having potentially beneficial mechanisms of action. The “fingerprint” data of known toxic compounds can be used to screen new compounds for toxicity.

Abstract: A computer method of detecting interacting DNA loci by constructing a contingency table from samples of a first trait and samples of a second trait. The samples of the first and second trait are associated with one of a plurality of genotype calls, each relating to an interaction between multiple DNA loci. The contingency table includes frequencies of each genotype call in the samples. Based on the contingency table, measuring the association between the plurality of genotype calls and the first and second traits. Classifying the genotype calls into a first group that is statistically associated with the first trait and a second group that is statistically associated with the second trait.

Abstract: Provided are methods for prognosing a clinical outcome in a subject with a hematological disorder comprising determining a 3D telomeres organization signature of a test sample cell from the subject, wherein the 3D telomeres organization signature of the test sample cell is indicative of the clinical outcome of the subject.

Abstract: In general, the presently disclosed technology relates to identification of cancer subtypes. More specifically, the technology relates to methods for determining molecular drivers of cancer and/or progression using a multivariate image data and statistical analysis of in-situ molecular markers and morphological characteristics in the same cells of a biological sample suspected of b cancer. This analysis takes place after a single acquisition that obtains the molecular and anatomic morphology data in parallel. The analysis compares specific morphological and molecular markers to known samples exhibiting particular genetic drivers of the cancer. This method provides statistical information that allows for an increased confidence in the identification of specific molecular drivers of the cancer.

Abstract: Exemplary systems, methods and non-transitory computer-accessible mediums are provided for generating a mosaic image of a large area of tissue with microscopic resolution by acquiring a plurality of rectangular-shaped long strips of images of the tissue and stitching the strips together along their lengths.