Patents Examined by Galina Yakovleva
  • Patent number: 10309973
    Abstract: The present invention provides compounds that are surrogates of post-translationally modified proteins and uses thereof. Numerous diseases are associated with post-translationally modified proteins that are difficult to obtain in homogenous form and in quantities needed for immunization and use as convenient standards, calibrators, and/or reference compounds that facilitate the detection and analysis of endogenous post-translationally modified proteins. The surrogate compounds of the invention typically comprise antigenic epitopes (one of which carries a post-translational modification) that are tethered by a flexible and hydrophilic linker. The resulting compound behaves like a surrogate of the post-translationally modified protein because it preserves the character of the included antigens and allows recognition by specific antibodies targeting the individual antigens.
    Type: Grant
    Filed: July 7, 2016
    Date of Patent: June 4, 2019
    Assignee: President and Fellows of Harvard College
    Inventors: Michael Chorev, Jose A. Halperin
  • Patent number: 10261074
    Abstract: Methods, systems, compositions include biocompatible polymer coated nanoceria that function as aqueous redox catalyst with enhanced activity at an acidic to moderately alkaline pH value between 1 and 8. The compositions are used as oxidizing agents for decomposition, decontamination or inactivation of organic contaminants, such as, pesticides and chemical warfare agents. Another use includes nanoceria as targetable nanocatalyst prepared by conjugating various targeting ligands to the nanoparticle coating to form a colorimetric or fluorescent probe in immunoassays and other molecule binding assays that involve the use of a molecule in solution that changes the color of the solution or emits a fluorescent signal, where localization of nanoceria to organs or tissue is assessed by treatment with an oxidation sensitive dye or other detection devices.
    Type: Grant
    Filed: November 10, 2014
    Date of Patent: April 16, 2019
    Assignee: University of Central Florida Research Foundation, Inc.
    Inventors: Jesus Manuel Perez, Atul Asati, Santimukul Santra, Charalambos Kaittanis, Sudip Nath
  • Patent number: 10253006
    Abstract: The current invention describes novel immunogens which are used in the production of novel antibodies with unique binding properties in that they cross-react with a variety of phenylpiperazine derivatives. These antibodies enable methods and kits to detect and/or determine phenylpiperazine derivatives (for example mCPP, TFMPP and MeOPP) in an in vitro sample which are advantageous over currently available analytical methods in terms of cost, ease of use, speed and sensitivity.
    Type: Grant
    Filed: April 15, 2016
    Date of Patent: April 9, 2019
    Assignee: Randox Laboratories Limited
    Inventors: Elouard Benchikh, Ivan McConnell, Philip Lowry, Peter Fitzgerald
  • Patent number: 10254297
    Abstract: Components for enabling immunodetection of methoxetamine are described including immunogens, haptens, antibodies and kits.
    Type: Grant
    Filed: July 1, 2016
    Date of Patent: April 9, 2019
    Assignee: Randox Laboratories Ltd.
    Inventors: Ivan McConnell, Philip Lowry, Elouard Benchikh, Peter Fitzgerald
  • Patent number: 10247739
    Abstract: The present invention relates to a method for the immunological measurement using a hapten and an antibody binding to the hapten as a reference antibody, and a device for the immunological measurement using the reference antibody. According to the present invention, the method and the device for the immunological measurement can be used in an immunoassay by reflecting surrounding environments, for example, by reflecting a numerical value in a test zone, which is changed by external factors such as temperatures of a sample and a device, an amount and components of an injected sample, variations of the device, and the like, in a reference zone and changing the numerical value in the test zone, and can be useful in minimizing analysis errors due to a low probability of causing a non-specific reaction with a material in the sample.
    Type: Grant
    Filed: October 31, 2014
    Date of Patent: April 2, 2019
    Assignee: SK TELECOM CO., LTD.
    Inventors: Sunyoung Park, Jeongryul Kim, Seong Woo Kim, Kum-Joo Shin, Ji-Chul Lee
  • Patent number: 10245279
    Abstract: Method for immobilizing a target microorganism or target chemical found in a mammal includes introducing into a gastrointestinal tract of the mammal immobilization particles including immobilization molecules capable of attaching to the target microorganism or the target chemical, which immobilization molecules are attached to one or more portions of a structure that is capable of inhibiting contact between tissues of the gastrointestinal tract and the target microorganisms or target chemicals attached to the immobilization molecules.
    Type: Grant
    Filed: January 20, 2016
    Date of Patent: April 2, 2019
    Assignee: NuBiome, Inc.
    Inventor: Brian C. Lue
  • Patent number: 10222388
    Abstract: The present invention relates to new vitamin D compounds which are bonded to a labelling group at the C3 stereocentre by means of a linker. The present invention further relates to a method for producing these vitamin D compounds and to the use of an intermediate for producing these compounds. The present invention also relates to a method for quantitatively determining vitamin D using a vitamin D compound according to the invention as a tracer. Furthermore, the present invention relates to a reagent for determining vitamin D, containing a compound according to the invention, and to the use thereof for determining vitamin D.
    Type: Grant
    Filed: August 1, 2014
    Date of Patent: March 5, 2019
    Assignee: ORGENTEC DIAGNOSTIKA GMBH
    Inventors: Vukic Soskic, Robert Poppe
  • Patent number: 10203326
    Abstract: A method of detecting a target substance, containing the steps of: incorporating labeling particles into a test liquid containing an analyte; heating the test liquid; irradiating the test liquid with excitation light, and detecting the target substance contained in the test liquid depending on a state of light emission of the labeling particles; wherein an aggregation state of the labeling particles is changed by the heating step; and wherein the labeling particles have a thermoresponsive polymer on a surface of a composite particle containing a magnetic material and a fluorescent material, and further have a biomolecule having properties of binding with the target substance.
    Type: Grant
    Filed: April 1, 2016
    Date of Patent: February 12, 2019
    Assignee: FURUKAWA ELECTRIC CO., LTD.
    Inventors: Masayuki Fukushima, Michio Ohkubo, Kazutomi Miyoshi, Masataka Nishida
  • Patent number: 10202466
    Abstract: Biosensors, compositions comprising biosensors, methods of producing biosensors, and methods of using biosensors are disclosed. The biosensors comprise a fluorogen-activating peptide and a blocking peptide. The fluorogen-activating peptide and blocking peptide are covalently linked through a peptide linker. The blocking peptide associates with the fluorogen-activating peptide thereby blocking an active domain of the fluorogen-activating peptide when the linker is in an unmodified state. The peptide linker may contain an amino acid sequence that is specifically recognized as a modification substrate by a cognate enzyme. The fluorogen-activating peptide and the blocking peptide at least partially disassociate when the linker is modified by an enzyme, thereby allowing the fluorogen-activating peptide to bind a cognate fluorogen and modulate a fluorescence signal.
    Type: Grant
    Filed: March 11, 2013
    Date of Patent: February 12, 2019
    Assignee: CARNEGIE MELLON UNIVERSITY
    Inventor: Peter B. Berget
  • Patent number: 10197583
    Abstract: Provided is a derivative of 22-hydroxycholesterol, 24S-hydroxycholesterol, 25-hydroxycholesterol, 26-hydroxycholesterol or 27-hydroxycholesterol. Also provided is a protein conjugated to the above derivative. Further provided is an antibody composition comprising antibodies that specifically bind to 22-hydroxycholesterol, 24S-hydroxycholesterol, 25-hydroxycholesterol, 26-hydroxycholesterol or 27-hydroxycholesterol. Additionally, a method of making antibodies that specifically bind to 22-hydroxycholesterol, 24S-hydroxycholesterol, 25-hydroxycholesterol, 26-hydroxycholesterol or 27-hydroxycholesterol is provided. Also, a method of assaying for 22-hydroxycholesterol, 24S-hydroxycholesterol, 25-hydroxycholesterol, 26-hydroxycholesterol or 27-hydroxycholesterol is provided. Additionally provided is a kit for detecting 22-hydroxycholesterol, 24S-hydroxycholesterol, 25-hydroxycholesterol, 26-hydroxycholesterol or 27-hydroxycholesterol.
    Type: Grant
    Filed: April 11, 2017
    Date of Patent: February 5, 2019
    Assignee: Enzo Life Sciences, Inc.
    Inventors: Michael C. Mullenix, Robert Elliot Zipkin, Jeffrey Kroll Adams, Wayne Forrest Patton, James J. Donegan
  • Patent number: 10197561
    Abstract: The invention is directed to a method for detecting a target moiety in a sample of biological specimens by: a) providing at least one conjugate with the general formula (I) Xn—P—Ym, with X is an detection moiety, P an enzymatically degradable spacer and Y an antigen recognizing moiety and n, m are integers between 1 and 100 and wherein X and Y are covalently bound to P; b) contacting the sample of biological specimens with at least one conjugate, thereby labeling the target moiety recognized by the antigen recognizing moiety Y; c) detecting the target moiety labeled with the conjugate with the detecting moiety X; and d) enzymatically degrading spacer P, thereby cleaving the detection moiety X from the conjugate. The method is useful to identify target moieties on the biological specimens. The biological specimens detected by the conjugate can be subsequently removed from the sample.
    Type: Grant
    Filed: November 18, 2015
    Date of Patent: February 5, 2019
    Assignee: Miltenyi Biotec, GmbH
    Inventors: Christian Dose, Jennifer Brieden, Stefan Miltenyi, Thomas Rockel, Veronika Gann
  • Patent number: 10196631
    Abstract: The invention is directed to a releasable conjugate comprising a biotinylated ligand having a biotin moiety, a ligand moiety (Ligand1) and a biotin-binding molecule (bbm) bound to the biotin moiety of the biotinylated ligand. The ligand moiety of the biotinylated ligand may be separated by a spacer group consisting of polyethylene glycol. Furthermore, the invention relates to a method for cleaving the releasable conjugate by providing biotin or streptavidin and an auxiliary release agent in a sufficient concentration to displace the biotin-binding molecule (bbm) from the biotin moiety of the biotinylated ligand and a method for separation of target cells from a cell sample utilizing the conjugate.
    Type: Grant
    Filed: May 27, 2016
    Date of Patent: February 5, 2019
    Assignee: Miltenyi Biotec GmbH
    Inventors: Jennifer Brieden, Christian Dose
  • Patent number: 10196449
    Abstract: There is disclosed an assay method for selectively detecting 1,25-dihydroxy-vitamin D in a biological fluid sample. According to the method of the invention, the pH of the test sample is adjusted to 6-9 and a receptor protein comprising the Ligand Binding Domain of Vitamin D Receptor (VDR-LBD) is added to the test sample, thereby obtaining the formation of a VDR-LBD/1,25-dihydroxyvitamin D complex in which the VDR-LBD portion is conformationally changed with respect to unbound VDR-LBD. The VDR-LBD/1,25-dihydroxyvitamin D complex is then detected by means of a capture moiety which is capable of specifically binding to VDR-LBD bound to 1,25-dihydroxyvitamin D. Also disclosed are an assay kit and an antibody for carrying out the method of the invention. The assay of the invention is preferably a sandwich immunoassay.
    Type: Grant
    Filed: January 27, 2014
    Date of Patent: February 5, 2019
    Assignee: DiaSorin S.p.A.
    Inventors: Joshua Soldo, Gregory Olson, Michael Lutterman, John Wall, Michael New, Hector Floyd Deluca, Fabrizio Bonelli
  • Patent number: 10180426
    Abstract: The present invention lies in the field of visualization and quantification of immobilized targets in samples using immunochemical means. The methods of the invention utilize an immunostaining system allowing visualizing single target units in samples as distinct dots. In particular, the invention relates to methods and reagents for visualization and quantification of molecular targets immunostained in histological samples and use of said method and reagents in medical diagnostic. However, the visualization and quantification methods of the invention are applicable to a variety of targets in different samples and allow precise quantifying both relative and absolute amounts thereof.
    Type: Grant
    Filed: November 8, 2011
    Date of Patent: January 15, 2019
    Assignee: DAKO DENMARK A/S
    Inventors: Jesper Lohse, Galina Skladtchikova
  • Patent number: 10175258
    Abstract: The invention describes methods and kits for detecting and determining current and future synthetic cannabinoids from the JWH and CP families. Unique antibodies derived from novel immunogens enable said methods and kits.
    Type: Grant
    Filed: November 7, 2014
    Date of Patent: January 8, 2019
    Assignee: RANDOX LABORATORIES LIMITED
    Inventors: Stephen Peter Fitzgerald, Paul John Innocenzi, Ivan Robert McConnell, Philip Andrew Lowry, Elouard Benchikh
  • Patent number: 10168325
    Abstract: Disclosed is a non-specific reaction inhibitor for use in an immunological measurement, comprising a complex of an antibody or a fragment of the antibody capable of specifically binding to a non-specific reaction factor, and a polymer. The non-specific reaction inhibitor can inhibit a non-specific reaction which may interfere with the accurate detection or quantification of a trace substance in an immunological measurement method.
    Type: Grant
    Filed: October 28, 2014
    Date of Patent: January 1, 2019
    Assignee: LSI MEDIENCE CORPORATION
    Inventors: Yoshikazu Takagi, Yuichi Shintani
  • Patent number: 10168323
    Abstract: The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture dimers), surfaces (e.g., displaying, capture ligands, capture proteins, or capture dimers), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.
    Type: Grant
    Filed: March 11, 2014
    Date of Patent: January 1, 2019
    Assignee: Promega Corporation
    Inventors: Carolyn W. Hitko, Robin Hurst, Thomas Kirkland, Dieter Klaubert, Poncho Meisenheimer, Nidhi Nath, Rachel Friedman Ohana, Paul Otto, Marjeta Urh, Harry Tetsuo Uyeda, Keith Wood
  • Patent number: 10150736
    Abstract: The invention relates to haptens, immunogens and secondary immunoreactive agents, to the use thereof for producing wide-spectrum antibodies against quinolone-type antibiotics, to the application thereof to immunochemical analysis techniques, and to a kit enabling the detection of said antibiotics in biological samples from food products of animal origin.
    Type: Grant
    Filed: November 21, 2011
    Date of Patent: December 11, 2018
    Assignee: Consejo Superior de Investigaciones Cientificas (CSIC)
    Inventors: Maria Pilar Marco Colas, Francisco José Sánchez Baeza, Daniel González Pinacho
  • Patent number: 10118996
    Abstract: A polyrotaxane containing an affinity binding group has been designed and prepared. The polyrotaxane of the invention can be used for characterization and determination of the three-dimensional structures of biological molecules, such as proteins.
    Type: Grant
    Filed: February 22, 2013
    Date of Patent: November 6, 2018
    Assignee: Purdue Research Foundation
    Inventors: David H. Thompson, Seok-Hee Hyun, Kyle J. Wright
  • Patent number: 10067127
    Abstract: An object of the present invention is to provide a method of detecting an analyte (antigen) in a sample by an antigen-antibody reaction with an antibody fragment including an antigen binding region for the analyte antigen (hereinafter “an antibody fragment comprising an antigen binding region for an analyte antigen” will simply be referred to as “an antibody fragment against antigen”), the method suppressing nonspecific reaction that is caused by antibody fragments. More specifically, provided is a method of detecting an analyte antigen in a sample by an antigen-antibody reaction with an antibody fragment against antigen, the method comprising the steps of: a) bringing a sample into contact with a denatured antibody fragment; and b) bringing the sample into contact with the antibody fragment immobilized on an insoluble carrier after the step of a), the method suppressing nonspecific reaction.
    Type: Grant
    Filed: September 30, 2013
    Date of Patent: September 4, 2018
    Assignee: SEKISUI MEDICAL CO., LTD.
    Inventors: Chie Kataoka, Hiroshi Takahashi, Tadaaki Yoshida, Yoshimasa Banba