Patents Examined by George P. Elliott
  • Humanized green fluorescent protein genes and methods
    Patent number: 5968750
    Abstract: Disclosed are synthetic and "humanized" versions of green fluorescent protein (GFP) genes adapted for high level expression in mammalian cells, especially those of human origin. Base substitutions are made in various codons in order to change the codon usage to one more appropriate for expression in mammalian cells. Recombinant vectors carrying such humanized genes are also disclosed. In addition, various methods for using the efficient expression of humanized GFP in mammalian cells and in animals are described.
    Type: Grant
    Filed: October 9, 1998
    Date of Patent: October 19, 1999
    Assignee: The University of Florida Research Foundation Inc.
    Inventors: Sergei Zolotukhin, Nicholas Muzyczka, William W. Hauswirth