Abstract: A membrane supported bioreactor arrangement and method for anerobic conversion of gas into liquid products including membrane modules having hollow fibers, each of the hollow fibers formed from an asymmetric membrane wall having a porous outer layer defining biopores for retaining a porous biolayer about the outer surface of the membrane wall and a less permeable hydration layer around the hollow fiber lumen; a membrane vessel for retaining the membrane modules in a process gas for formation of the biolayer on the outer surface of the hollow fiber wall by interaction of microorganisms with a process gas and for the production of a liquid product, wherein the membrane vessel retains the membrane modules in a common horizontal plane; provides a seal between contents of the membrane tank and ambient atmosphere; and includes a liquid supply conduit for communicating the process liquid with the hollow fiber lumens of the hollow fibers.
Abstract: Embodiments described include methods for maintaining the pH and alkalinity during industrial fermentation processes are described that foster optimal microorganism activities throughout the duration of the fermentation process. Magnesium based buffering agents having sufficient buffering capacity and applied at sufficient concentration levels required to neutralize the acidic content of fermentation fluids during the fermentation process cycle are detailed. These magnesium based buffering agents control fermenter processes without causing extreme pH swings to maximize the production of desired fermentation products.
Abstract: A process for simultaneous saccharification and fermentation of a cellulosic solids fraction extracted from a lignocellulosic feedstock. The viscosity of the cellulosic solids fraction is reduced by intermixing with a liquid carbohydrate stream. A suitable liquid carbohydrate stream is a de-lignified liquids fraction that was previously separated from the solids fraction during processing of the lignocellulosic feedstock. Alternatively, the viscosity of the solids fraction may be reduced by commingling with a liquid carbohydrate stream comprising one or more monosaccharides. The reduced-viscosity cellulosic solids fraction is then commingled with a fermentative microbial inoculant and a cellulosic biomass-degrading enzyme composition. The commingled mixture is maintained in a pressurized reaction vessel under elevated temperatures to enable simultaneous enzymatic hydrolysis of the cellulosic solids to monosaccharides and fermentation of the monosaccharides to produce an ethanolic beer.
Type:
Grant
Filed:
July 3, 2008
Date of Patent:
November 15, 2011
Inventors:
Alex Berlin, Edward Kendall Pye, Donald O'Connor
Abstract: The present invention relates to a method of producing polyhydroxyalkanoates (PHAs) using Bacillus sp. with succinate as a carbon source. The PHAs comprise more than 95% of poly(3-hydroxyvalerate-co-4-hydroxyvalerate) (P3HV-co-P4HV).
Abstract: The present invention relates to an antibacterial agent containing 7,10-dihydroxy-8(E)-octadecenoic acid (referred as DOD) as an active ingredient. DOD produced by the method of the present invention can be effectively used as an antibacterial agent because it has a wide spectrum of antibacterial activity against various microorganisms.
Type:
Grant
Filed:
June 17, 2008
Date of Patent:
November 8, 2011
Assignee:
Kyung Pook National University Industry-Academic Cooperation Foundation
Abstract: The invention is directed to a process for the production and recovery of chemicals, in particular hydrocarbons, from a fermentation medium, wherein solvent impregnated carriers are used. Accordingly, the invention provides a process for the production of hydrocarbon from a fermentation liquid comprising: —forming said hydrocarbon from said fermentation liquid using a biocatalyst;—contacting said fermentation liquid with a solvent impregnated porous carrier, which solvent impregnated porous carrier has a density that is different from said fermentation liquid, whereby said formed hydrocarbon is sorbed by said solvent impregnated carrier; —regenerating said solvent impregnated carrier, whereby a stream of said hydrocarbon is obtained; and optionally, recycling said regenerated solvent impregnated carrier.
Type:
Grant
Filed:
March 1, 2005
Date of Patent:
November 1, 2011
Assignee:
Nederlandse Organisatie voor toegepast-natuurwetenschappelijk Onderzoek TNO
Inventors:
Jan de Bont, Paulus Josephus Theodorus Bussmann, Earl Lawrence Vincent Goetheer, Zhuo Hornstra-Xu, Dionne Josephine Irving, Jan Izaak Walpot, Johan Alexander Vente
Abstract: The present invention provides a method for producing preparations comprising soluble arabinoxylans as co-products of ethanol production through fermentation of whole-grain cereals. The method results in preparations that are highly enriched in soluble arabinoxylans, which can be used as a food, beverage, or feed ingredients.
Type:
Grant
Filed:
July 2, 2007
Date of Patent:
October 11, 2011
Assignee:
Fugeia NV
Inventors:
Willem Broekaert, Christophe Courtin, Jan Delcour
Abstract: A method of biotreating and recovering metal values from metal-bearing refractrory sulfide ore using a nonstirred surface bioreactor is provided. According to the method, the surface of a plurality of coarse substrates is coated with a solid material to be biotreated to form a plurality of coated coarse substrates. The coarse substrates have a particle size greater than about 0.3 cm and the solid material to be biotreated has a particle size less than about 250 ?m. A nonstirred surface reactor is then formed by stacking the plurality of coated coarse substrates into a heap or placing the plurality of coated coarse substrates into a tank so that the void volume of the reactor is greater than or equal to about 25%. The solid material is biotreated in the surface bioreactor until the undesired compound in the solid material is degraded to a desired concentration.
Abstract: A method for inexpensive and efficient PHA biosynthesis includes operating a sequencing bioreactor in alternating phases of nutrient deprivation and carbon feedstock deprivation to select for robust PHA-producing microbes. Preferably, the bioreactor is operated in a non-sterile manner with mixed cultures of methanotrophs. The method also preferably uses periodic biomass-wasting (PHA harvesting) at the end of the carbon feed phase, gradually lengthening the time period of carbon deprivation phase to create a penalty for rapid PHA degradation and incentive for PHA accumulation. Also, bacterial enrichment cultures may be introduced periodically. The PHA-accumulating bacteria are preferably grown on common anaerobic degradation products, specifically volatile fatty acids, such as acetate and propionate, and methane gas. The PHA has useful applications in bioplastics and other products.
Type:
Grant
Filed:
June 24, 2009
Date of Patent:
October 4, 2011
Assignee:
The Board of Trustees of the Leland Standford Junior University
Abstract: A method of producing a retinyl ester compound comprising subjecting a composition comprising retinyl or a retinyl ester and a fat or oil of animal or vegetable origins to enzyme catalysed trans-esterification in solvent free conditions to produce a retinyl ester.
Type:
Grant
Filed:
November 6, 2008
Date of Patent:
October 4, 2011
Assignee:
Unilever Home & Personal Care USA, division of Conopco, Inc.
Inventors:
John Anthony Bosley, Clive Roderick Harding, Christopher Rawlins, Julia Sarah Rogers, Ian Richard Scott
Abstract: A method for the co-production of silica and at least one other useful industrial chemical such as ethanol, comprises the steps of: pre-treating siliceous plant matter derived from plants, such as horsetail weeds from the genus Equisetum, to create a feedstock having exposed cellulose; placing the feedstock in a reactor containing a biological agent effective to break down the cellulose into at least one useful organic chemical reaction product and a silica-containing co-product; separating the at least one useful organic chemical product from the reactor; separating the silica-containing co-product from the reactor; and refining the silica-containing co-products into silica or other industrially useful silicon containing products. The biological agent may be an anaerobic thermophyllic bacteria, enzymes, or a co-mixture of enzymes and yeast.
Abstract: A method of biotreating a solid material including an organic compound is provided. According to the method, a nonstirred bioreactor having a void volume of greater than or equal to 25% with a mixture including a solid material to be biotreated and a plurality of coarse substrates having a particle size greater than or equal to about 0.6 cm is formed. The solid material to be biotreated includes an organic material. The mixture includes sufficient coarse substrates to provide the reactor with at least 100 square meters of surface area per cubic meter of reactor space. The solid material in the bioreactor is biotreated until the organic material within the bioreactor is reduced to a desired concentration.
Abstract: Pediococcus bacteria having enhanced tolerance to butanols have been isolated. The bacteria are useful for the fermentative production of butanol.
Type:
Grant
Filed:
December 4, 2007
Date of Patent:
September 13, 2011
Inventors:
Michael G. Bramucci, Helene M. A. Kane, Vasantha Nagarajan
Abstract: A resource production system using a byproduct from the production of ethanol for efficiently producing biogas and fertilizer from the byproducts of ethanol production. The resource production system using a byproduct of a production of ethanol generally includes a plurality of processes for producing an inorganic renewable fertilizer, such as struvite and a non-fossil fuel source of energy, such as biogas, by using various types of byproducts produced by the ethanol plant, such as but not limited to whole stillage, thin stillage and thin stillage solubles. The process also produces an organic fertilizer, such as biosolids and a liquid stream suitable for further processing to produce recyclable water at the ethanol plant.
Abstract: An object of the present invention is to provide a process according to which 5-ene-3-one or 3,6-dione derivatives of sterols can be synthesized with a better yield, as well as a process for production of lipid metabolism improvers, foods, drinks, and animal feeds containing the 5-ene-3-one or 3,6-dione derivatives of sterols. The process for production of 5-ene-3-one or 3,6-dione derivatives of sterols of the present invention involves preparing a culturing solution containing an Arthrobacter bacterium, which is a microorganism exhibiting a cholesterol oxidase activity, and then, adding to the culturing solution an equivalent amount of hexane so as to prepare a bilayer solution consisting of an aqueous layer and a hydrocarbon-based solvent layer. Thereafter, sterol, or a derivative thereof, is made as a substrate contained therein, a reaction performed with cholesterol oxidase at 30° C. for 2 days, and, ethanol then added to separate a hydrocarbon-based solvent layer.
Abstract: The present invention provides a microbial hydrogen-producing process, comprising: providing at least one Clostridium microbe and at least one Bacillus microbe; and co-culturing said at least one Clostridium microbe and said at least one Bacillus microbe in a fermentation culture system to produce hydrogen. The present invention also provides a microbial hydrogen-producing system, characterized by that the system comprises at least one Clostridium microbe and at least one Bacillus microbe, and it uses an organic waste medium as the substrate to perform a hydrogen-producing fermentation having high efficiency, high stability, and high reproducibility.
Abstract: A method of separating a polymer from a biomass containing the polymer, includes contacting the biomass with a solvent system, the solvent system including a solvent for the polymer and a precipitant for the polymer, to provide a residual biomass and a solution that includes the polymer, the solvent for the polymer and the precipitant for the polymer; and applying a centrifugal force to the solution and residual biomass to separate at least some of the solution from the residual biomass; where, the polymer is a polyhydroxyalkanoate; the precipitant comprises at least one alkane; the solvent for the polymer is selected from the group consisting of ketones, esters and alcohols and combinations thereof; and the biomass comprises a slurry of the biomass and water.
Type:
Grant
Filed:
April 15, 2010
Date of Patent:
August 23, 2011
Assignee:
Metabolix, Inc.
Inventors:
Johan van Walsem, Luhua Zhong, Simon S. Shih
Abstract: The present invention generally relates to novel processes for protein purification in high salt solutions such as cell culture broth by increasing the dynamic binding capacity of a resin with the addition of polyethylene glycol.
Abstract: There are provided polyunsaturated fatty acids-containing oils/fats or triglycerides with high digestion and absorption properties and resistance to oxidative damage, which are suitable for applications in fields such as modified milk for infants, food products and healthy foods and/or supplements, produced by transesterification of polyunsaturated fatty acids-containing oils/fats or triglycerides with vegetable oils/fats or triglycerides using 1,3-position specific type lipases.
Abstract: A method for the production of ethanol and a modified animal feed is provided. The method replaces the starch in known corn-based animal feed with biomass fiber treated to make it more digestible by animals. The process includes wherein the pericarp and germ are removed from the corn kernel and processed for by-products. The starch and protein are also removed and separated. The starch is then fermented and distilled to ethanol and stillage. The bioavailable modified animal feed comprises the pericarp and germ removed from corn kernels and optionally by-products of the pericarp and germ processing, and lignocellulosic materials. The modified animal feed may optionally include energy materials such as animal and vegetable fats, vegetable soapstocks, or glycerin, and combinations thereof.
Type:
Grant
Filed:
January 15, 2009
Date of Patent:
August 16, 2011
Assignee:
Archer Daniels Midland Company
Inventors:
Charles Abbas, Thomas P. Binder, Kyle E. Beery, Michael J. Cecava, Perry H. Doane, David P. Holzgraefe, Leif P. Solheim