Abstract: Assemblies and methods for disinfecting surfaces using ultraviolet (UV) light are disclosed. In one aspect, a UV light-emitting assembly comprises a plurality of UV light emitters, and first and second UV light emitter supports seating the UV light emitters. A first heat sink is affixed to the first UV light emitter support and a second heat sink is affixed to the second UV light emitter support. A thermally conductive and electrically insulating plate contacts the first heat sink and the second heat sink.

Abstract: An apparatus and method for preparing nanoemulsions from an oil phase and an aqueous phase, wherein the oil phase can be essential oils, such as damask rose essential oil. The apparatus includes a homogenization unit having a homogenization tank and a sonication unit comprising a sonication tank. The dispersion of oil phase and aqueous phase is passed between the homogenization unit and sonication unit multiple times to prepare the nanoemulsion.

Abstract: A microorganism activation system for activating microorganisms prior to application of microorganisms to agricultural products includes a first container, a second container, and a disruptable dividing member. The first container includes a stabilized microbial culture therein. The second container includes an aqueous rehydration solution therein. The disruptable dividing member separates the stabilized microbial culture in the first container from the aqueous rehydration solution in the second container. Disrupting the dividing member releases the stabilized microbial culture into the second chamber to contact the aqueous rehydration solution. Contact between the stabilized microbial culture and the aqueous rehydration solution re-initiates cellular activity of the microorganisms.

Abstract: Disclosed are methods and apparatus for disinfecting an interior space in a vehicle such as an aircraft. A method includes determining critical levels of heated air in the interior space of the vehicle required to neutralize effects of at least one predetermined pathogen, providing an apparatus for supplying heated air and positioning the apparatus in the interior space, activating the apparatus to supply the heated air to achieve the determined critical levels of the heated air in the interior space of the vehicle, maintaining the achieved critical levels of the heated air in the interior space for a determined critical time period, and optionally cooling the interior space following completion of the disinfecting process. A disinfecting apparatus may include a heated air generator, blower, controller and optional humidity generator, and may be implemented as a cart for traversing an aisle.

Abstract: Devices, systems, and methods can be used for the automated production of dendritic cells (DC) from dendritic cell progenitors, such as monocytes obtained from peripheral blood, and the automated generation of immunotherapeutic products from those dendritic cells, all within a closed system. The invention makes it possible to obtain sufficient quantities of a subject's own DC for use in preparing and characterizing vaccines, for activating and characterizing the activation state of the subject's immune response, and to aid in preventing and/or treating cancer or infectious disease.

Abstract: In the cell culture device, a volume of a space defined by a diaphragm of the diaphragm pump and a plane at an outer edge of the diaphragm is 1 cm3 or more and 20 cm3 or less, in a case where an angle formed by a straight line connecting a foot of a perpendicular line from the apex to the plane to each point in the diaphragm and by the perpendicular line is denoted by an angle A, a thickness H of the diaphragm is within a range of 0.5 mm or more and 1.5 mm or less where the angle A is from 0° to 75°, and in a case where a thickness of the diaphragm at the apex is denoted by HT, the thickness H satisfies a relationship of 1?HT/H?1.75 at any point where the angle A is from 0° to 75°.

Abstract: A device for simulating a function of a tissue includes a first structure, a second structure, and a membrane. The first structure defines a first chamber. The first chamber includes a matrix disposed therein and an opened region. The second structure defines a second chamber. The membrane is located at an interface region between the first chamber and the second chamber. The membrane includes a first side facing toward the first chamber and a second side facing toward the second chamber. The membrane separates the first chamber from the second chamber.

Abstract: The present set of embodiments relate to a system, method, and apparatus for separating cells from a suspension using a gravity driven cell separation device. The apparatus may include a chamber that is separated into a gas compartment and a liquid compartment allowing for transfer of gas through a membrane into the liquid compartment where the cell suspension flows during operation. The embodiments are designed to maximize the gravitational separation effect on the suspension while mitigating the loss of surface area through which gas transfer can occur through a membrane to provide nutrients to cells in the suspension by tilting the cell separation device on two or more axes.

Abstract: It was difficult to create a thin bio sample embedded in an embedding material without using an expensive microtome. The present invention provides a medical device having a space for assisting in the embedding of a bio sample in an embedding material, the medical device being provided with a cover part and a base part having a recessed section in which the space is created by covering the cover part, wherein the base part is provided with a flow passage having an inlet section that is in fluid communication with the space, and the flow passage is configured such that when a force is applied to the medical device so that the volume of the space decreases, the embedding material flows out while preventing a positional misalignment of the bio sample inside the embedding material.

Abstract: A method of preparing viable stromal and mesenchymal stem cells from adipose tissue that produces high quality and high counts of stem cells with a low risk of contamination. The apparatus provides ultrasonic waves through a constant temperature bath to the tissue held in a sterile sonication container such as a test tube or jar. No sonication probe touches the tissue or the cells during the process. The stem cells produced are ready to be administered to a patient.

Abstract: A support housing for a flexible bioprocess bag, the housing comprising at least one side wall having a first segment and a second segment, the first segment movable in relation to the second segment between an open and a closed position, wherein at least a supporting part of the housing is translatable from an operating position to a bag loading position, wherein the supporting part comprises one or more retainers for holding the flexible bioprocess bag upon re-translation of the supporting part to the operating position and movement of the first segment to the closed position, and wherein the first segment comprises a first drive unit for connecting and driving a first mixing unit in the flexible bioprocess bag.

Abstract: A cell culture vessel has a wall and cell culture surface having a plurality of microcavities for culturing cells in three-dimensional conformation, referred to as “spheroids”. The inner surface of the wall and the cell culture surface define a cell culture chamber of the vessel. The wall is attached to the cell culture surface in a way that does not provide flat surfaces on or around the cell culture surface so that the vessel provides an environment suitable for the production of a homogeneous population of three-dimensional cell clusters, or spheroids.

Abstract: A method of disinfecting using a light diffusing fiber includes optically coupling a light source to a light diffusing optical fiber having a core, a cladding surrounding the core, an outer surface, and a plurality of scattering structures positioned within the core, the cladding, or both the core and the cladding. The method further includes positioning the light diffusing optical fiber in optical engagement with a pathogen sample and directing light output by the light source into the light diffusing optical fiber for a first time interval. The scattering structures scatter light propagating along the light diffusing optical fiber toward the outer surface and a portion of the light diffuses through the outer surface thereby irradiating the pathogen sample with light having an average power density of about 5 mW/cm2 to about 30 mW/cm2 at a wavelength from about 380 nm to about 495 nm for an exposure time from about 2 hours to about 24 hours.

Abstract: In some aspects, the invention relates to selectively permeable cell culture vessel storage containers and their methods of use. In some embodiments, the containers comprise a gas-permeable membrane that is selectively impermeable to water vapor.

Abstract: This application relates to Pseudomonas fluorescens N1, and usage therefor in solid waste recycling based on full bionic simulation combined with a microorganism. Light, temperature, gas and heat conditions in cyclic transformation of organic carbon in soil are simulated, and solid waste is mixed and stacked in the soil or between hills, wherein the solid waste is basically blended according to the carbon-nitrogen ratio, alkalinity or acidity, and water content of the solid waste, and no turning is performed according to the principle of anaerobic fermentation. By introducing high-carbon, high-salt, alkaline or acidic liquid waste using a bionic head cover during stacking, the anaerobic fermentation process is controlled to stop at an acid production stage so that organic acids produced are fully mixed with the solid waste, and the nutrients in the solid waste are released by using acidic materials and thus become nutritional elements for acidic material chelation.

Abstract: The invention discloses a first connection unit having a plurality of sensor surfaces and which is adapted to be aseptically connected to a second connection unit mounted e.g. on a flexible bioreactor bag.

Abstract: A system includes an airflow passage and an air purification element. The airflow passage is configured to confine a flow of air and direct the flow of air toward a space. The air purification element is positioned in the airflow passage and configured to produce a peroxide to oxidize contaminants in air flowing through the airflow passage. The air purification element includes a peroxide-generating structure and a matrix containing a peroxide-activating catalyst configured to activate peroxide produced. The air purification element is configured to produce the peroxide from a water vapor and oxygen in the air within the airflow passage when energized by an energy source, and the matrix is configured to allow the contaminants to contact peroxide activated by the peroxide-activating catalyst.

Abstract: A bioculture meat device includes a culture medium conditioning tank (1), a cell proliferation tank (2), a muscle separating tank (3), a compression forming device (4), and a control system. The control system is divided into a culture medium regulation and control system (51) for controlling the culture medium conditioning tank (1), a cell proliferation control system (52) for controlling the cell proliferation tank (2), and a muscle collecting and shaping control system (53) for controlling the muscle separating tank (3) and the compression forming device (4). Different from traditional manual production, the device controls a culture environment through automatic equipment, realizes automatic integration from culturing to processing a finished product, improves the production capacity, and reduces the cost.

Abstract: A tissue culture vessel including a graft support tray (200) and a box having a lid (400) and a base which engages and retains the tray. The tray has two operational states: a first operational state in which a floor of the tray is slightly raised with respect to a floor of the base and a second operational state in which the floor of said tray descends to contact the floor of the base.

Abstract: A UV module for deactivating biologically contaminated surfaces in an interior of a device includes: a carrier; a radiation source affixed to the carrier and configured to emit UV radiation; and a control unit connected to the radiation source, wherein the control unit is configured to receive and process at least one information item to activate the radiation source to emit UV radiation based on the at least one information item. A system comprises such a UV module wherein the device is configured for the handling and/or examination of samples comprising biological material and has an interior into which the UV module can be introduced. A method for deactivating biologically contaminated surfaces in an interior of a device for the handling and/or examination of samples comprising biological material includes using the device.