Abstract: This invention provides recombinant cells and methods for producing terpenes and terpenoids by increasing production or accumulation or both of isoprenoid precursors thereof.
Type:
Grant
Filed:
August 1, 2017
Date of Patent:
January 29, 2019
Assignee:
EVOLVA SA
Inventors:
Thomas Oestergaard Tange, Michael Naesby, Christophe Folly, Fanny Delegrange, Jens Houghton-Larsen, Simon Carlsen
Abstract: A method of producing bi-functional fatty acids comprising introducing into a host cell or organism, which comprises one or more ?- or ?-1 functionalized acyl-CoAs, and expressing therein a KASIII, which can use one or more of the ?- or ?-1 functionalized acyl-CoAs as a substrate; a method of producing a ?-1 hydroxy branched fatty acid, a ?-1 branched fatty acid, or a combination thereof by culturing a mutant E. coli, which does not express a functional KASIII from the endogenous fabH gene and expresses a phaA and a phaB and a functional exogenous KASIII; and a mutant E. coli, a method of making the mutant, a culture comprising the mutant, and a composition comprising ?-1 hydroxy branched fatty acids, a ?-1 branched fatty acids, or a combination thereof obtained from the culture.
Type:
Grant
Filed:
November 30, 2015
Date of Patent:
January 22, 2019
Assignees:
Iowa State University Research Foundation, Inc., OmegaChea
Abstract: The invention relates to recombinant host cells having at least one integrated polynucleotide encoding a polypeptide that catalyzes a step in a pyruvate-utilizing biosynthetic pathway, e.g., pyruvate to acetolactate conversion. The invention also relates to methods of increasing the biosynthetic production of isobutanol, 2,3-butanediol, 2-butanol or 2-butanone using such host cells.
Type:
Grant
Filed:
August 7, 2017
Date of Patent:
January 22, 2019
Assignee:
Butamax Advanced Biofuels LLC
Inventors:
Larry Cameron Anthony, Lori Ann Maggio-Hall
Abstract: The present invention relates to a modified cellobiose dehydrogenase (CDH) or its functional flavodehydrogenase domain having glucose oxidation activity and a reduced maltose oxidation activity as compared to the unmodified CDH or its functional flavodehydrogenase domain, nucleic acids encoding said enzyme or domain, electrodes with said enzyme or domain and methods of producing and using the same.
Type:
Grant
Filed:
March 6, 2013
Date of Patent:
January 22, 2019
Assignee:
DIRECTSENS GMBH
Inventors:
Roland Ludwig, Christoph Sygmund, Wolfgang Harreither, Roman Kittl, Alfons Felice
Abstract: Engineered microorganisms are provided that convert gaseous substrates, such as producer gas, into limonene. In some embodiments, limonene is pumped out of the cell via an efflux pump. In some embodiments, limonene, produced as described herein, is converted through catalytic dimerization into jet fuel. Producer gas used in the processes described herein for production of limonene may be derived from sources that include gasification of waste feedstock and/or biomass residue, waste gas from industrial processes, or natural gas, biogas, or landfill gas.
Type:
Grant
Filed:
October 20, 2016
Date of Patent:
January 15, 2019
Assignee:
Kiverdi, Inc.
Inventors:
Christer Jansson, Cody A. Marcus Carr, John S. Reed
Abstract: Described herein are genetically-modified microorganisms for producing medium chain lauric acid and/or dodecanedioic acid and methods of using the microorganisms. The microorganisms contain a nucleic acid encoding an Umbellularia californica lauroyl ACP-thioesterase (BTE) operably linked to a promoter.
Type:
Grant
Filed:
December 29, 2016
Date of Patent:
January 8, 2019
Assignee:
INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE
Abstract: The present invention relates to a method of producing cellulosic sugar from biomass by effective impurity removal and sugar separation, and more particularly to a method for producing cellulosic sugar from biomass. The method includes pretreating the biomass so as to be separated into a solid and a liquid; subjecting the separated liquid to monomerization, concentration and adsorption processes, thereby obtaining xylose; and subjecting the separated solid to second pretreatment, saccharification and lignin separation processes, thereby obtaining glucose. The method allows fermentation inhibitory substances to be removed from pretreated biomass in a cost- and energy-effective process, and is useful for effective production of cellulosic sugar.
Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.
Type:
Grant
Filed:
September 12, 2016
Date of Patent:
December 11, 2018
Assignee:
Genomatica, Inc.
Inventors:
Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Priti Pharkya
Abstract: The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in microorganisms by engineering a microorganism for increased carbon flux towards mevalonate production in the following enzymatic pathways: (a) citrate synthase, (b) phosphotransacetylase, (c) acetate kinase, (d) lactate dehydrogenase, (e) malic enzyme, and (f) pyruvate dehydrogenase such that one of more of the enzyme activity is modulated. In addition, production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids can be further enhanced by the heterologous expression of the mvaE and mvaS genes (such as, but not limited to, mvaE and mvaS genes from the organisms Listeria grayi DSM 20601, Enterococcus faecium, Enterococcus gallinarum EG2, and Enterococcus casseliflavus).
Type:
Grant
Filed:
July 22, 2015
Date of Patent:
November 27, 2018
Assignees:
Danisco US Inc., The Goodyear Tire & Rubber Company
Inventors:
Zachary Q. Beck, Michael C. Miller, Caroline M. Peres, Yuliya A. Primak, Jeff P. Pucci, Derek H. Wells
Abstract: The present invention discloses an industrial scale method to obtain 1-kestose by the use of a recombinant fructosyltransferase (FTF), isolated from Festuca arundinacea, expressed constitutively in a non-saccharolytic yeast. In this invention, the recombinant FTF type sucrose:sucrose 1-fructosyltransferase (1-SSTrec) is produced constitutively, stable and at high yield, both in the culture supernatant and in intact cells of the host Pichia pastoris. Hence, the invention additionally provides a method for 1-SST production at industrial scale. The recombinant enzyme is then used for mass production of short-chain fructooligosaccharides (FOS), specifically 1-kestose, from sucrose. The method of the present invention establishes conditions that allow conversion rates where the synthesized FOS constitute above 55% (w/w) of the total sugars in the reaction mixture and the 1-kestose content reaches values higher than 90% (w/w) of the total FOS fraction.
Type:
Grant
Filed:
September 18, 2013
Date of Patent:
November 20, 2018
Assignee:
CENTRO DE INGENIERIA GENÉTICA Y BIOTECNOLOGIA
Inventors:
Enrique Rosendo Pérez Cruz, Lázaro Hernández García, Duniesky Martínez García, Luis Enrique Trujillo Toledo, Carmen Menéndez Rodríguez, Alina Sobrino Legón, Ricardo Ramírez Ibañez, Gumersindo Feijoo Costa, Juan Manuel Lema Rodicio
Abstract: The present invention relates to a process for the development of a highly efficient solid state matrix by the activation of acrylate based polymer resin having specialized functional groups with 1,1-Carboxyl diimidazole for immobilizing biologically active macromeloecules such as oxidases, in particular plant oxidases and the most preferred being tea polyphenol oxidase through indirect covalent bonding/cross linking on such activated polymer resin support, are thermally stable, gives very high number of turnovers in vitro (“n” times) with tea substrate forming exclusive product Theaflavins without any loss of biological activity and leaving the product remaining in vitro with adherence to matrix rendering the matrix safe towards product poisoning and subsequent partial or complete loss of biological activity of the matrix bound enzyme system and thus well adapted to and well suited biorectors based on such systems.
Type:
Grant
Filed:
March 21, 2007
Date of Patent:
November 20, 2018
Assignee:
COUNCIL OF SCIENTIFIC AND INDUSTRIAL RESEARCH
Abstract: A method for the in vivo production of 1,3-butadiene from 2,4-pentadienoate is described (FIG. 1). Enzymes capable of decarboxylating 2,4-pentadienoate to 1,3-butadiene have been discovered. Recombinant expression of these newly discovered enzymes has resulted in the engineering of microorganisms capable of producing 1,3-butadiene when cultured in the presence of 2,4-pentadienoate. 1,3-butadienoate is an important monomer used in the manufacturing of rubbers and plastics. This invention will help to enable the biological production of 1,3-butadiene from renewable resources such as sugar, for example.
Type:
Grant
Filed:
August 13, 2014
Date of Patent:
November 13, 2018
Assignee:
ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
Inventors:
David Nielsen, Shawn Pugh, Rebekah McKenna
Abstract: Materials and methods are described to produce xylitol from a mixture of hemicellulosic sugars by several routes. Examples include either as a direct co-product of a biorefinery or ethanol facility, or as a stand-alone product produced from an agricultural or forestry biomass feedstock including using, e.g. ethanol waste streams.
Type:
Grant
Filed:
January 14, 2011
Date of Patent:
October 23, 2018
Assignees:
The Board of Trustees of the University of Illinois, ZuChem, Inc.
Inventors:
Huimin Zhao, Nikhil Unni Nair, Michael Racine, Ryan Woodyer
Abstract: The disclosure provides a nitrilase from Arabis alpina, which belongs to genus Arabis, family brassicaceae. The disclosure further provides the encoding gene, vector, recombinant bacterial strain, and the application in the manufacturing of (S)-3-cyano-5-methylhexanoic acid. The wet resting cells containing nitrilase Aa-Nit can kinetically resolve racemic IBSN at 1.2 M with a 42% conversion rate in 15 hr and >99% ee value. The disclosure provides a regio- and stereoselective method for the preparation of (S)-3-cyano-5-methylhexanoic acid. This method provides an atom economical, mild, environmental friendly industrial method to manufacture (S)-3-cyano-5-methylhexanoic acid.
Type:
Grant
Filed:
August 28, 2017
Date of Patent:
October 16, 2018
Assignees:
ZHEJIANG CHIRAL MEDICINE CHEMICALS CO. LTD., ZHEJIANG UNIVERSITY OF TECHNOLOGY
Abstract: Described herein are packages for storing and dispensing multi-part tooth whitening formulations, comprising a deformable material configured to form two or more sealed chambers, e.g., wherein the first chamber contains a low viscosity liquid solution comprising an enzyme having perhydrolytic activity, and the second chamber contains a peroxide source and a at least one acyl donor substrate. Particular multi-part tooth whitening formulations using this principle and methods of use thereof are also provided.
Type:
Grant
Filed:
December 18, 2012
Date of Patent:
October 16, 2018
Assignee:
Colgate-Palmolive Company
Inventors:
Thomas Boyd, Guofeng Xu, Richard Adams, Robert Pierce, Derek Samaroo, David Viscio, Kari A. Fosser, Robert Dicosimo, Hong Wang
Abstract: Compositions and methods for the treatment of cancer are described, and, more preferably, to the treatment of cancers that do not express, or are otherwise deficient in, argininosuccinate synthetase, with enzymes that deplete L-Arginine in serum. In one embodiment, the present invention contemplates an arginase protein, such as a human Arginase I protein, comprising at least one amino acid substitution and a metal cofactor, said protein comprising an increased catalytic activity when compared with a native human Arginase I.
Abstract: The present invention provides a method of producing a polymer, which comprises the step of performing a polymerization reaction using, as a starting material, an organic acid obtained by allowing a microorganism or a treated cell thereof to act on an organic raw material, wherein said microorganism has an ability to produce an organic acid and has been modified so as to produce less aromatic carboxylic acid as compared to an unmodified strain.
Type:
Grant
Filed:
May 31, 2016
Date of Patent:
September 18, 2018
Assignee:
Mitsubishi Chemical Corporation
Inventors:
Shuichi Yunomura, Gou Takahashi, Yoshiaki Mori
Abstract: Provided is an acid-tolerant yeast cell, a method of producing an organic acid by using the yeast cell, and a method of producing the yeast cell resistant to acid.
Type:
Grant
Filed:
July 28, 2016
Date of Patent:
August 21, 2018
Assignee:
SAMSUNG ELECTRONICS CO., LTD.
Inventors:
Hunsu Chu, Hwayoung Cho, Jinhwan Park, Dongsik Yang, Hongsoon Rhee, Kwangmyung Cho
Abstract: It is an object of the present invention to provide a bacterial strain that can decrease the amount of an intermediate Compound P converted into Metabolite M and efficiently accumulate Compound P in a medium that is not supplemented with Metabolite M or the final product generated from Metabolite M. The present invention provides a prokaryotic organism having all features (a) to (d) as defined in the specification so as to accumulate Compound P by regulating expression level of Enzyme X that converts Compound P as an intermediate metabolite into Metabolite M in a biosynthetic pathway in which Metabolite M indispensable for the growth is produced from a carbon source.
Abstract: The invention provides a unique subset of GH30 subfamily 8 xylanases (GH30-8) with endo-?-1,4-xylanase activity, compositions comprising an effective amount of the GH30-8 xylanases, methods of synthesis and methods of use thereof.
Type:
Grant
Filed:
July 9, 2015
Date of Patent:
August 7, 2018
Inventors:
Franz St. John, Diane Dietrich, Merritt E. Casey Crooks