Patents Examined by J. Christopher Ball
  • Patent number: 12038405
    Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.
    Type: Grant
    Filed: May 26, 2023
    Date of Patent: July 16, 2024
    Assignee: Life Technologies Corporation
    Inventors: Mark Milgrew, Jonathan Rothberg, James Bustillo
  • Patent number: 12038404
    Abstract: The present application discloses improved multiple-use sensor arrays for determining the content of various species in samples of biological origin, in particular in the area of point-of-care (POC) testing for blood gases. The multiple-use sensor array is arranged in a measuring chamber, and the sensor array comprises two or more different ion-selective electrodes including a first ion-selective electrode (e.g. an ammonium-selective electrode being part of a urea sensor), wherein the first ion-selective electrode includes a membrane comprising a polymer and (a) a first ionophore (e.g. an ammonium-selective ionophore) and (b) at least one further ionophore (e.g. selected from a calcium-selective ionophore, a potassium-selective ionophore, and a sodium-selective ionophore), and wherein the first ionophore is not present in any ion-selective electrode in the sensor array other than in the first ion-selective electrode.
    Type: Grant
    Filed: June 29, 2021
    Date of Patent: July 16, 2024
    Assignee: Radiometer Medical ApS
    Inventors: Thomas Kjaer, Lone Michelsen, Poul Ravn Sorensen, Hans Peter Blaabjerg Jakobsen
  • Patent number: 12038407
    Abstract: Dual nucleic acid and protein isoform measurements are performed on low starting cell numbers (e.g. equivalent to the number of blastomeres composing early embryonic development stages (morula and blastocysts)), comprising integrating fractionation polyacrylamide gel electrophoresis (fPAGE) of 10-100 cells with off-chip analysis of nucleic acids in the nuclei.
    Type: Grant
    Filed: May 24, 2021
    Date of Patent: July 16, 2024
    Assignee: The Regents of the University of California
    Inventors: Amy E. Herr, Lin He, Andrew J. Modzelewski, Elisabet Rosàs-Canyelles
  • Patent number: 12031942
    Abstract: Various embodiments include a method of ascertaining the ammonia concentration and the nitrogen monoxide concentration in the exhaust gas stream from a motor vehicle comprising: measuring a pumping current at least three times; and determining an ammonia concentration and a nitrogen monoxide concentration based on three successive pumping current measurements.
    Type: Grant
    Filed: May 16, 2019
    Date of Patent: July 9, 2024
    Assignee: VITESCO TECHNOLOGIES GMBH
    Inventor: Hong Zhang
  • Patent number: 12031936
    Abstract: A gas sensor element has an electrolyte layer, a first insulator, a second insulator, a measurement gas chamber, and a reference gas chamber. The electrolyte layer includes a holding plate and a solid electrolyte body. The first insulator is laminated on one side of the electrolyte layer, and the second insulator is laminated on the other side of the electrolyte layer. At least a part of the boundary portion between the placement hole and the solid electrolyte body is sandwiched between a first sandwiching portion of the first insulator and a second sandwiching portion of the second insulator.
    Type: Grant
    Filed: February 22, 2021
    Date of Patent: July 9, 2024
    Assignee: DENSO CORPORATION
    Inventors: Masatoshi Ikeda, Shota Hagino, Makoto Ito, Daisuke Kawai
  • Patent number: 12031935
    Abstract: The present invention provides a process and method for the early detection and diagnosis of disease by reading and decoding volatile organic compounds (VOCs) for signatures associated with a specific disease. From its outset, each disease begins producing its own unique set of volatile organic compounds. For many diseases, this early-stage detection may be many months or years before noticeable symptoms. The VOC emissions when analyzed, result in a “signature” that identifies and distinguishes the developing, or at later stages, the developed disease. The device assays non-invasively obtained biosamples in real-time to output a VOC based signature that, when correlated with data in a disease signature library, identifies one or more diseases associated with the sample.
    Type: Grant
    Filed: April 29, 2021
    Date of Patent: July 9, 2024
    Inventor: Richard Postrel
  • Patent number: 12025579
    Abstract: The application provides a method of detecting an analyte in a sample. The method comprises disposing a binding agent in an electrochemical compartment. The binding agent is configured to bind to an interfering species. The method further comprises disposing a solution comprising a sample in the electrochemical compartment. The sample comprises an analyte and the interfering species. The method then comprises applying a voltage across first and second spaced apart electrodes disposed in the solution, and thereby causing a current to flow through the solution between the electrodes. Finally, the method comprises measuring the current and/or voltage and thereby detecting the analyte.
    Type: Grant
    Filed: March 12, 2019
    Date of Patent: July 2, 2024
    Assignee: UNIVERSITY OF LEICESTER
    Inventors: Sergey Piletsky, Francesco Canfarotta, Antonio Guerreiro, Giovanna Marrazza, Riccardo Rapini
  • Patent number: 12025581
    Abstract: A method of detecting and/or discriminating mismatched or complementary nucleic acids using a field-effect transistor (FET). The FET comprises source and drain electrodes formed on substrate and separated by a channel that includes a thin semiconducting film. One or more nucleic acid molecules are immobilized to thin semiconducting film. The FET includes a gate electrode in contact with solution containing the sample (or located on the surface of the device). Samples possibly containing target nucleic acid are exposed to the FET of the biosensor device and current response is measured after samples containing target nucleic acid have been exposed to the FET of the biosensor device. Measured current response in the FET is used to detect and/or discriminate whether target nucleic acid is present as well as complementary or mismatched. Measured current response may also be used to differentiate among different mismatches.
    Type: Grant
    Filed: April 11, 2019
    Date of Patent: July 2, 2024
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Paul S. Weiss, Anne M. Andrews, Kevin M. Cheung
  • Patent number: 12017225
    Abstract: Methods and apparatus for providing an isolated single cell are provided. In one disclosed arrangement, a test body of liquid is formed on a substrate surface. A contact angle between the test body of liquid and the substrate surface is lower than an equilibrium contact angle. An optical image of the test body of liquid is analysed to determine whether one and only one cell is present in the test body of liquid.
    Type: Grant
    Filed: August 8, 2019
    Date of Patent: June 25, 2024
    Inventor: Edmond Walsh
  • Patent number: 12019045
    Abstract: An ion sensor includes a substrate, at least one ion selective electrode deposited on the substrate, and a reference electrode deposited on the substrate. The sensor may further include an insulating layer placed over the ion selective electrode and the reference electrode and having openings for the ion selective electrode and the reference electrode, a microfluidic layer placed over at least part of the insulating layer, and a cover layer placed over the microfluidic layer. The reference electrode includes reference electrode material deposited on the substrate and a combination of a polymer and a chloride-containing salt deposited on the reference electrode material.
    Type: Grant
    Filed: October 10, 2019
    Date of Patent: June 25, 2024
    Assignee: MX3 Diagnostics, Inc.
    Inventors: Chathurika Darshani Abeyrathne, You Liang, Efstratios Skafidas
  • Patent number: 12013368
    Abstract: An embodiment provides a method for measuring at least one characteristic of an aqueous sample, including: introducing an aqueous sample into a measurement device comprising one or more electrodes; oxidizing a transition metal to produce a higher valent metal by applying an electrical potential between an anode and a cathode of the measurement device; oxidizing, using the higher valent metal as a catalyst, a material within the aqueous sample; measuring a characteristic of the aqueous sample based upon the oxidized material, using a measurement device selected from the group consisting of: an electrochemical measurement device and an optical measurement device; and optimizing the electrical potential and at least one reagent delivered to the measurement device based on the measurement of the characteristic. Other aspects are described and claimed.
    Type: Grant
    Filed: July 24, 2019
    Date of Patent: June 18, 2024
    Assignee: HACH COMPANY
    Inventors: Vishnu Rajasekharan, Russell Young, Richard Leggett, Seamus O'Mahony
  • Patent number: 12011294
    Abstract: Described herein are variations of an analyte monitoring system, including an analyte monitoring device. For example, an analyte monitoring device may include an implantable microneedle array for use in measuring one or more analytes (e.g., glucose), such as in a continuous manner. The microneedle array may include, for example, at least one microneedle including a tapered distal portion having an insulated distal apex, and an electrode on a surface of the tapered distal portion located proximal to the insulated distal apex. At least some of the microneedles may be electrically isolated such that one or more electrodes is individually addressable.
    Type: Grant
    Filed: December 21, 2022
    Date of Patent: June 18, 2024
    Assignee: Biolinq Incorporated
    Inventors: Alan Campbell, Sirilak Sattayasamitsathit, Jared Rylan Tangney, Thomas Arnold Peyser, Joshua Ray Windmiller
  • Patent number: 12004858
    Abstract: Methods and analyte sensors including at least a first working electrode having a first active area thereon, and performing a dip coating operation to deposit a bilayer membrane upon the first working electrode and the first active area. The bilayer may include an inner layer having a first membrane polymer and an outer layer having a second membrane polymer, the first membrane polymer and the second membrane polymer differing from one another. The dip coating operation may comprise one or more first dips in a first membrane formulation to form the inner layer of the bilayer membrane and one or more second dips in a second membrane formulation to form the outer layer of the bilayer membrane upon the inner layer.
    Type: Grant
    Filed: January 18, 2021
    Date of Patent: June 11, 2024
    Assignee: Abbott Diabetes Care Inc.
    Inventors: Stephen Oja, Tianmei Ouyang, Hyun Cho, Lam N. Tran, Benjamin J. Feldman, Ashwin Kumar, Namvar Kiaie
  • Patent number: 11994525
    Abstract: A membrane protein analysis substrate including an electron microscope grid having a plurality of through-holes; a lipid bilayer membrane that is provided to cover at least one of the plurality of through-holes; and membrane proteins that are retained in a part planarly overlapping the through-holes of the lipid bilayer membrane, wherein the lipid bilayer membrane has a lipid monolayer, and wherein the lipid monolayer is larger than the through hole in a plan view, adheres to the grid, and constitutes a part of the lipid bilayer membrane.
    Type: Grant
    Filed: March 30, 2023
    Date of Patent: May 28, 2024
    Assignee: KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION
    Inventor: Atsushi Shimada
  • Patent number: 11992842
    Abstract: Air-matrix digital microfluidics (DMF) apparatuses and methods of using them. These methods and apparatuses may include the use of a liquid wax coating material and/or pinning the encapsulated reaction droplet within the air gap using pinning features. Any of these methods may also include separating the liquid wax from an encapsulated aqueous droplet, e.g., using an oil absorbent wick to selectively separate the liquid oil or wax from the aqueous droplet by adsorbing and/or absorbing the liquid wax into the absorbent wick while leaving the aqueous droplet behind.
    Type: Grant
    Filed: November 3, 2020
    Date of Patent: May 28, 2024
    Assignee: mirOculus Inc.
    Inventors: Mais Jehan Jebrail, Mathieu Gabriel-Emmanuel Chauleau, Poornasree Kumar, Eduardo Cervantes, Foteini Christodoulou, Nikolay Sergeev, Spencer Seiler, Alejandro Tocigl Domeyko, Ana Eugenia Carvajal
  • Patent number: 11981557
    Abstract: The present invention includes one or more nanopores in a SixNy membrane comprising a monoprotic surface termination, methods of making, and methods of using the one or more nanopores, where the one or more nanopores are a chemically-tuned controlled dielectric breakdown (CT-CDB) nanopore membrane, wherein the CT-CDB allows for long-term stability of measurements in the presence of only electrolyte (open pore current stability) and ability to support many molecular detection events. In addition, the CT-CBD has pore that unclog spontaneously, in response to voltage cessation or application, or both.
    Type: Grant
    Filed: April 16, 2021
    Date of Patent: May 14, 2024
    Assignee: SOUTHERN METHODIST UNIVERSITY
    Inventors: Y. M. Nuwan D. Y. Bandara, Buddini I. Karawdeniya, Jugal Saharia, Min Jun Kim, Jason Rodger Dwyer
  • Patent number: 11980849
    Abstract: Disclosed herein are compositions and methods that involve inserting connector protein channels of bacteriophage DNA packaging motors into copolymeric membranes via liposome-polymer fusion, which can be used as nanopore sensors for biomedical applications such as high throughput protein sequencing or cancer diagnosis. For example, disclosed are compositions comprising a copolymeric membrane into which a connector protein channel of a bacteriophage packaging motor has been inserted.
    Type: Grant
    Filed: February 8, 2019
    Date of Patent: May 14, 2024
    Assignee: Ohio State Innovation Foundation
    Inventors: Peixuan Guo, Zhouxiang Ji, Lakmal Jayasinghe, Michael Jordan
  • Patent number: 11981963
    Abstract: Methods for analyzing a nucleic acid molecule are described. Methods may include attaching the nucleic acid molecule to a particle having a first characteristic dimension. In addition, methods may include applying an electric field through an aperture to move the particle to the aperture. Also, methods may include applying a voltage across a first electrode and a second electrode. Further, methods may include contacting a portion of the nucleic acid molecule to both the first electrode and the second electrode within the aperture, where the portion may include a nucleotide. In addition, methods may include measuring a current through the first electrode, the portion of the nucleic acid molecule, and the second electrode, where the measured current runs in a direction parallel to a longitudinal axis of the aperture. Also, methods may include identifying the nucleotide of the portion of the nucleic acid molecule based on the current.
    Type: Grant
    Filed: March 21, 2023
    Date of Patent: May 14, 2024
    Assignee: Roche Sequencing Solutions, Inc.
    Inventor: Yann Astier
  • Patent number: 11977064
    Abstract: Various embodiments of the teachings herein include methods of ascertaining the nitrogen oxide concentration and a nitrogen oxide ratio in the exhaust gas stream from a motor vehicle comprising: measuring a pumping current and ascertaining the nitrogen oxide concentration and the nitrogen oxide ratio from at least three successive pumping current measurements.
    Type: Grant
    Filed: May 16, 2019
    Date of Patent: May 7, 2024
    Assignee: VITESCO TECHNOLOGIES GMBH
    Inventor: Hong Zhang
  • Patent number: 11971384
    Abstract: Electrophoresis is used to identify presence of a target compound in a patient sample based on a charge state of the compound and a label. The charge state of the compound correlates to a total net charge of a binder conjugated to the compound. The bound complex or “bound complex” with the label is then applied to the electrophoresis substrate. An electric potential is applied to the substrate for a time period and causes the labeled bound complex to migrate toward the electrode with opposite charge of the labeled bound complex at a migration velocity to form a migration pattern over the time period. At some time during or at the end of the time period, the labeled bound complex produces a bound complex band as a result of its migration across the substrate. The presence of the compound is identified based on the labeled bound complex band and one or both of the migration pattern and the migration velocity.
    Type: Grant
    Filed: October 18, 2021
    Date of Patent: April 30, 2024
    Assignee: Hemex Health, Inc.
    Inventors: Peter Galen, Ariane Elizabeth Erickson, David Richard Bell, Matthew Christian Lind, Tyler Witte, Umut Atakan Gurkan