Abstract: Hybrid cell line for production of monoclonal antibody to an antigen found on normal human monocytes and granulocytes. The hybrid is formed by fusing splenocytes from immunized BALB/cJ mice with P3X63Ag8Ul myeloma cells. Diagnostic and therapeutic uses of the monoclonal antibody are also disclosed.

Abstract: The invention relates to a process for the incorporation of foreign DNA into chromosomes of dicotyledonous plants by infecting the plants or incubating plant protoplasts with Agrobacterium bacteria, which contain one or more plasmids, wherein bacteria are used which contain at least one plasmid having the vir-region of a Ti (tumour inducing) plasmid but no T-region, and at least one other plasmid having a T-region with incorporated therein foreign DNA but no vir-region, as well as to a Agrobacterium bacteria wherein at least one plasmid which has the vir-region of a Ti (tumour inducing) plasmid but no T-region and at least one other plasmid which has a wild type T-region with incorporated in it foreign DNA but no vir-region.

Abstract: Hybrid cell line for production of monoclonal antibody to an antigen found on normal human monocytes and granulocytes. The hybrid is formed by fusing splenocytes from immunized BALB/cJ mice with P3X63Ag8Ul myeloma cells. Diagnostic and therapeutic uses of the monoclonal antibody are also disclosed.

Abstract: Cells of Pseudomonas bacteria having a high nitrile hydratase activity can be obtained in a high yield by adding sequentially to a culture medium at least one compound selected from the group consisting of propionitrile, isobutyronitrile, propionamide, and isobutyramide in the process of cultivation of Pseudomonas bacteria capable of producing nitrile hydratase.

Abstract: This invention provides a semi-continuous fermentation process which is operated in a repeated fed-batch mode to maintain cell bioconversion productivity at a high level without product inhibition of enzymatic activity. The process is illustrated by the bioconversion of toluene or catechol via the ortho pathway to muconic acid which accumulates in the fermentation medium in a quantity up to about 50 grams per liter.

Abstract: Promoter probe vectors for determining the presence of efficient promoter regions on DNA segments, which segments permit microbiological expression of genetic information. The promoter probe vector comprises replicons active in E. coli and B. subtilis, a structural gene for B-galactosidase and at least one endonuclease restriction site suitable for insertion of promoter-containing DNA fragments.

Abstract: An efficient process design for purifying large quantities of specific antibodies by affinity chromatography is disclosed. By utilizing the process strategy described, large scale production of highly purified hybridoma antibodies and other proteins becomes feasible.The initiation of the process centers on production of hybridoma proteins which bind to a commonly available inexpensive protein such as human serum albumin (HSA) with which large quantities of a protein such as mouse immunoglobulin can be isolated by affinity chromatography. The mouse immunoglobulin is covalently linked to an inert matrix, such as Sepharose beads and this affinity reagent is used to purify a large quantity of a hydridoma protein such as rat anti-mouse IgG antibody. This rat anti-mouse IgG antibody can then be linked to Sepharose beads and can be used as a general affinity reagent for the purification of any mouse immunoglobulin.

Abstract: An enhanced labelling complex for localizing markers on a prepared tissue section is disclosed. The complex includes both avidin components and biotinylated macromolecular components at a ratio preselected to provide a complex which is sufficiently large to include a large number of labels, and sufficiently small to penetrate the tissue section. The markers are localized by first introducing a biotinylated link into the tissue section and thereafter exposing the section to the labelling complex.

Abstract: An improvement in immunological methods for quantitative detection of Dirofilaria immitis antibodies in a fluid sample comprising a treatment of the sample with Toxocara canis-derived antigens.

Abstract: The preparation and use of monoclonal antibodies to human renal tumor cells is described. The monoclonal antibodies bind to glycoproteins of 160Kd, 120Kd and 115Kd, a glycolipid, a HLA heavy chain, group A blood and group B blood antigens.

Abstract: A method is described for the manufacture of commercially useful alpha-halo and alpha-dihalo ketones and aldehydes from alkynes by enzymatic reaction. The alkyne is acted upon in a reaction mixture comprising a halogenating enzyme, an oxidizing agent, and a halide ion source.

Abstract: An identification technique for micro-organisms in which a dilute solution of a culture medium containing an unknown micro-organism has added thereto an emissive agent such as a radioactive amino acid to produce a mix of emissive products that depends on the metabolic mechanism of the micro-organism. After a predetermined incubation period, the reaction is arrested and the solution layered onto a gel plate where it is subjected to electrophoresis. The plate is then autoradiographed by exposing the gel to a sensitive photographic film for a period sufficient to produce thereon a characteristic band pattern functioning as an identifier for the micro-organism. Identification may be effected by comparing the identifier for the unknown with a collection of identifiers for known micro-organisms to find a match with one of these known identifiers.

Abstract: A method for the identification of microorganisms comprises adding to a sample containing an unknown microorganism an emissive agent such as a radioactive amino acid to produce a mix of emissive products that depends on the metabolic mechanism of the microorganism. After incubation, the reaction is arrested and the emissive products are separated, as by electrophoresis on a gel plate. The plate may then be autoradiographed by exposure to a photographic film to produce on the latter a characteristic band pattern functioning as an identifier for the microorganism. Identification can be effected by comparing the identifier for the unknown with a collection of identifiers for known microorganisms to find a match with one of these known identifiers. The comparison may be carried out by scanning the unknown identifier to produce a signal which is compared with signals representing known identifiers stored in a computer.

Abstract: A method for cultivating, under alkaline conditions, microorganisms in a culture medium containing, as a carbon source, the extracted liquor or spent liquor derived from alkaline pulping is presented. In this cultivation, organic acids contained in the extracted liquor or spent liquor can be effectively utilized. Typical microorganisms cultured are bacteria belonging to the genera Bacillus, Arthrobacter, Corynebacterium and Brevibacterium.

Abstract: A method for eliminating turbidity in a biological fluid by combining said fluid with a surfactant and an enzyme is disclosed as well as a diagnostic reagent formulation for that purpose.

Abstract: A tryptic peptide is produced by partial tryptic digestion of purified, high molecular size colon-specific antigen-p (CSAp), to produce a lower molecular size antigen carrying the CSAp antigenic determinant. The tryptic peptide is used to produce monospecific anti-CSAp antibodies.

Abstract: The present invention prepares protein hydrolyzates by reacting selected protein material with at least one proteolytic enzyme and recovering the low molecular weight protein material by ultrafiltration while recycling the high molecular weight material and proteolytic enzyme for further hydrolysis. A series of ultrafilters of varying permeability may also be used.