Abstract: A method for isolating specific viable cell types from surrounding organ tissue is provided. The method entails the use of sonication in conjunction with tissue dissociating agents to free the cells of interest. A specific application of the method is the isolation of the insulin producing tissue of the pancreas, the islets of Langerhans. The method results in a high yield of islets that maintain a high level of viability.
Type:
Grant
Filed:
August 10, 1998
Date of Patent:
December 3, 2002
Assignee:
Novocell, Inc.
Inventors:
Brad Gray, Monty Kahn Baird, Francis Lamberti