Abstract: A rapid technique is disclosed for the identification of enteric bacteria which elaborate cholera-related heat-labile enterotoxin antigens which have the ability to cause diarrheal disease in man or animals. The invention includes a sensitized carrier particle for use as a reagent in an agglutination test for heat-labile enterotoxins and the use of said reagent in immunological determinations.

Abstract: This invention describes a process for immuno-chemical quantitative determination of immunologically active substances. The method involves a first incubation of a sample containing the active substance with a labelled binder, which contains an antibody or antibody fragment. After complexing has taken place, solid phase bound active substance identical to the substance being quantitatively determined is added. The solid phase bound substance binds with free binder, and the solid and liquid phases are separated. A second antibody which is specific either to antibody or the solid phase antibody-substance complex is then added to the liquid phase. This second antibody is non cross-reactive with individual complex components. The amount of labelled first antibody bound to second antibody is then determined.

Abstract: A biochemical detection method in which a ligand is bound to a surface, an aqueous solution containing an antiligand is brought into contact therewith whereby antiligand becomes bound to ligand, the aqueous solution is separated from the thus bound antiligand, and the antiligand is detected using a detection means associated therewith, for example using amplified enzyme-linked immunoassay. The surface is treated with at least one agent for limiting non-specific binding of either the ligand or the antiligand with other substrates, which agent is(i) a surfactant containing an aromatic residue and having an HLB number of at least 16,(ii) a zwitterionic surfactant, or(iii) a solution containing a salt of a polyvalent anion, in a concentration of at least 100 mM.Preferably, all three agents are used simultaneously. The three agents may be used during the detection procedure in solutions containing the antiligand, in washing solutions used during the method, or in solutions used to pre-treat the plate.

Abstract: Reagent for immunoassay, ligand binding assay and ligand receptor assay in which luciferin is covalently bonded to a molecule having biological activity for bonding to a particular biologically active group of a material and method in which the luciferin and biologically active molecule conjugate is added to a material having a group for combination with the biological activity of the conjugated molecule, the mixture is incubated to bond the material to the reagent through the biological activity and the product of the bonding is reacted with luciferase to produce bioluminescence having intensity dependent on the concentration of the material being assayed.

Abstract: In a homogeneous assay, binder supported on a solid support, having a sac lysing agent conjugated thereto, is contacted with analyte and tracer comprised of sacs containing a detectable marker. The sacs of the bound tracer portion are lysed to release marker to determine analyte in the sample.

Abstract: A solution containing from about 0.25% to about 5% hydrolyzed ovalbumin by weight provides surprising thermal stabilization of monoclonal antibodies.

Abstract: A method and serological reagent for determining antigens or antibodies with enhanced sensitivity and specificity techniques acts in an interdependent manner in one diagnostic unit of two separately acting components. This action can be simultaneous or consecutive and the combined effect inhibits "lectin-like" factors or "unspecific antibodies" and detects specific antibodies or antigens.