Patents Examined by James Ketter
  • Heterodimeric receptor libraries using phagemids
    Patent number: 7118866
    Abstract: Filamentous phage comprising a matrix of cpVIII proteins encapsulating a genome encoding first and second polypeptides of an antogenously assembling receptor, such as an antibody, and a receptor comprised of the first and second polypeptides surface-integrated into the matrix via a filamentous phage coat protein membrane anchor domain fused to at least one of the polypeptides.
    Type: Grant
    Filed: October 18, 2002
    Date of Patent: October 10, 2006
    Assignee: The Scripps Research Institute
    Inventors: Angray Kang, Carlos F. Barbas, III, Richard A. Lerner
  • Method for electro-permeabilization of individual cellular and organellar structures and use thereof
    Patent number: 7109034
    Abstract: The invention relates to a method for permeabilization of a cell structure consisting of a single cell, an intracellular structure or an organelle comprising the following steps: (a) microelectrodes, preferably two carbon fiber electrodes or hollow fiber electrodes, are provided, (b) the microelectrodes are connected to a power supply, (c) the electrodes, individually controlled by high-graduation micromanipulators, are placed close to the cell structure at an appropriate inter-electrode distance, and (d) a highly focused electric field of a strength sufficient to obtain electroporation is applied between the electrodes. The method may be used in order to transfer cell impermeant solutes, such as drugs or genes, into the cell structure or out of the cell structure, in biosensors, in the treatment of tumours and neurodegenerative diseases and in the study of biophysical processes.
    Type: Grant
    Filed: December 19, 2002
    Date of Patent: September 19, 2006
    Assignee: Cellectricon AB
    Inventors: Owe Orwar, Anders Lundqvist, Peter Eriksson, Daniel Chiu
  • Method for ligating nucleic acids and molecular cloning
    Patent number: 7109178
    Abstract: The invention provides methods of covalently joining nucleic acid molecules and methods of molecular cloning. The methods provide either sequential or simultaneous ligation of flanking or vector nucleic acid molecules to nucleic acid insert molecules by topoisomerase and DNA ligase. The methods provide for directional and non-directional covalent joining and cloning of nucleic acid molecules.
    Type: Grant
    Filed: January 25, 2002
    Date of Patent: September 19, 2006
    Assignee: Stratagene California
    Inventors: Henry Ji, Alan Greener, Joseph A. Sorge, John Bauer, Richard Gibbs, Carsten-Peter Carstens
  • Methods and compositions comprising DNA damaging agents and p53
    Patent number: 7109179
    Abstract: The present invention relates to the use of tumor suppressor genes in combination with a DNA damaging agent or factor for use in killing cells, and in particular cancerous cells. A tumor suppressor gene, p53, was delivered via a recombinant adenovirus-mediated gene transfer both in vitro and in vivo, in combination with a chemotherapeutic agent. Treated cells underwent apoptosis with specific DNA fragmentation. Direct injection of the p53-adenovirus construct into tumors subcutaneously, followed by intraperitoneal administration of a DNA damaging agent, cisplatin, induced massive apoptotic destruction of the tumors. The invention also provides for the clinical application of a regimen combining gene replacement using replication-deficient wild-type p53 adenovirus and DNA-damaging drugs for treatment of human cancer.
    Type: Grant
    Filed: February 23, 2004
    Date of Patent: September 19, 2006
    Assignee: Board of Regents, the University of Texas System
    Inventors: Jack A. Roth, Toshiyoshi Fujiwara, Elizabeth A. Grimm, Tapas Mukhopadhyay, Wei-Wei Zhang, Laurie B. Owen-Schaub
  • N-terminal and C-terminal markers in nascent proteins
    Patent number: 7101662
    Abstract: This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents.
    Type: Grant
    Filed: October 9, 2001
    Date of Patent: September 5, 2006
    Assignee: Ambergen, Inc.
    Inventors: Kenneth J. Rothschild, Sadanand Gite, Jerzy Olejnik
  • Electrofusion microelectrode
    Patent number: 7101703
    Abstract: The present invention is directed to an electrofusion microelectrode used in the alignment, manipulation, fusion, or electroporation of cells. This device is particularly useful for transplantation of cells and cellular components.
    Type: Grant
    Filed: February 28, 2002
    Date of Patent: September 5, 2006
    Assignee: Cornell Research Foundation, Inc.
    Inventor: Gianpiero D. Palermo
  • Peptide vector
    Patent number: 7101700
    Abstract: The present invention relates to a peptide vector comprising a leader peptide, a linker DNA, and a desired gene. This peptide vector can achieve gene transfer without cell specificity and does not induce host immune responses.
    Type: Grant
    Filed: February 8, 2002
    Date of Patent: September 5, 2006
    Inventor: Young-Jin Chae
  • Polyampholytes for delivering polyions to a cell
    Patent number: 7098030
    Abstract: An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
    Type: Grant
    Filed: July 26, 2004
    Date of Patent: August 29, 2006
    Assignee: Mirus Bio Corporation
    Inventors: David B. Rozema, Vladimir G. Budker, James E. Hagstrom, Vladimir Trubetskoy, Jon A. Wolff, Sean D. Monahan, Paul M. Slattum
  • Compositions and methods for drug delivery using pH sensitive molecules
    Patent number: 7098032
    Abstract: An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
    Type: Grant
    Filed: January 28, 2005
    Date of Patent: August 29, 2006
    Assignee: Mirus Bio Corporation
    Inventors: Vladimir S. Trubetskoy, James E. Hagstrom, Vladimir G. Budker, Jon A. Wolff, David B. Rozema, Sean D. Monahan
  • Eukaryotic signal sequences for prokaryotic expression
    Patent number: 7094579
    Abstract: The present invention generally relates to methods and compositions for expressing proteins or polypeptides in prokaryotic hosts using eukaryotic signal sequences.
    Type: Grant
    Filed: February 13, 2002
    Date of Patent: August 22, 2006
    Assignee: XOMA Technology Ltd.
    Inventors: Jeff Gray, Joe Buechler, Uday Kumar Veeramallu
  • Formation of polyampholytes in the presence of a polyion
    Patent number: 7094605
    Abstract: Polyampholyte are able to condense nucleic acid to form small complexes which can be utilized in the delivery of nucleic acid to mammalian cells. The polyampholytes can be formed prior to interaction with nucleic acid or they can be formed in the presence of nucleic acid. Stabilized polycation/nucleic acid complexes can be modified to reduce the positive charge of the polycation and add targeting ligands without destabilizing the complex. The resultant particles retain their small size and are more effective in delivery of nucleic acid to cells in vivo.
    Type: Grant
    Filed: January 28, 2005
    Date of Patent: August 22, 2006
    Assignee: Mirus Bio Corporation
    Inventors: Darren H. Wakefield, David B. Rozema, Jon A. Wolff, Vladimir Trubetskoy, James E. Hagstrom, Vladimir G. Budker, Jason Klein, So Wong
  • L-Lysine-producing corynebacteria and process for the preparation of L-lysine
    Patent number: 7094584
    Abstract: The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.
    Type: Grant
    Filed: March 19, 2004
    Date of Patent: August 22, 2006
    Assignee: Degussa AG
    Inventors: Caroline Kreutzer, Stephan Hans, Mechthild Rieping, Bettina Möckel, Walter Pfefferle, Lothar Eggeling, Hermann Sahm, Miroslav Patek
  • Methods and compositions for controlling biofilm development
    Patent number: 7094394
    Abstract: A method of cleaning or protecting surfaces by treatment with compositions comprising N-(3-oxododecanoyl)-L-homoserine lactone (OdDHL) blocking compounds and/or N-butyryl-L-homoserine lactone (BHL) analogs, either in combination or separately.
    Type: Grant
    Filed: July 1, 2002
    Date of Patent: August 22, 2006
    Assignees: Montana State University, University of Rochester, University of Iowa Research Foundation
    Inventors: David G. Davies, John William Costerton
  • Poly ADP-ribose polymerase gene and it uses
    Patent number: 7094950
    Abstract: Compositions and methods for influencing the metabolic state of plant cells are provided. The compositions comprise poly ADP-ribose polymerase genes and portions thereof, particularly the maize poly ADP-ribose polymerase gene as well as antisense nucleotide sequences for poly ADP-ribose polymerase genes. The nucleotide sequences find use in transforming plant cells to alter the metabolic state of the transformed plants and plant cells.
    Type: Grant
    Filed: August 28, 2003
    Date of Patent: August 22, 2006
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Pramod Mahajan, Zhuang Zuo
  • Method for directed packaging of molecular substances in protein shells
    Patent number: 7091028
    Abstract: The invention relates to a method for packaging molecular substances in protein shells, comprising the following steps: binding a protein shell fragment, via a first region to a matrix; bringing the protein shell fragment bound to the matrix into contact with the molecular substance, in order to bind the molecular substance to a second region of the protein shell fragment; separating the protein shell fragment with the bonded molecular substance, or the part thereof which contains the bonded molecular substance, from the matrix and assembling the separated protein shell fragments, or the part thereof which contains the bonded molecular substance, with other protein shell fragments to form a protein shell, whereby the separation and assembly can be carried out in any order.
    Type: Grant
    Filed: November 3, 2000
    Date of Patent: August 15, 2006
    Assignee: ACGT ProGenomics AG
    Inventors: Gerald Boehm, Dirk Esser, Ulrich Schmidt
  • Non-invasive gene targeting to ocular cells
    Patent number: 7090864
    Abstract: Liposomes containing therapeutic genes are conjugated to multiple targeting agents to provide transport of the encapsulated gene across the blood-retinal barrier and the plasma membrane of ocular cells. Once across the blood-retinal barrier and ocular cell membrane, the encapsulated gene expresses the encoded therapeutic agent within the ocular cells to provide diagnosis and/or treatment of disease.
    Type: Grant
    Filed: December 19, 2001
    Date of Patent: August 15, 2006
    Assignee: The Regents of the University of California
    Inventor: William M. Pardridge
  • Materials and methods for the modification of plant lignin content
    Patent number: 7087426
    Abstract: Novel isolated polynucleotides and polypeptides associated with the lignin biosynthetic pathway are provided, together with genetic constructs including such sequences. Methods for the modulation of lignin content, lignin structure and lignin composition in target organisms are also disclosed, the methods comprising incorporating one or more of the polynucleotides of the present invention into the genome of a target organism.
    Type: Grant
    Filed: June 18, 2002
    Date of Patent: August 8, 2006
    Assignees: Agrigenesis Biosciences Ltd., Rubicon Forests Holdings Limited
    Inventors: Leonard N. Bloksberg, Ikka Havukkala
  • Methods for detecting polypeptides regulating signal transduction pathways
    Patent number: 7087382
    Abstract: A method of detecting a polypeptide capable of regulating a transduction pathway and an expression library for use with such method are provided. The method is effected by introducing into cells endogenously expressing a trans acting regulator of the transduction pathway the expression library which includes a plurality of expression constructs each encoding a polypeptide and a reporter molecule expressible in the presence of a trans acting regulator of the transduction pathway. A level of expression within a predetermined range of the reporter is indicative of regulation of the transduction pathway by the polypeptide.
    Type: Grant
    Filed: October 25, 2000
    Date of Patent: August 8, 2006
    Assignee: Stil Biotechnologies Ltd.
    Inventor: Sylvie Luria
  • Methods for large scale protein production in prokaryotes
    Patent number: 7087412
    Abstract: The invention belongs to the field of protein production in prokaryotic cells. The invention relates to methods for the production of recombinant DNA-derived heterologous protein in prokaryotic cells, wherein the heterologous protein is secreted extracellularly as an active and correctly folded protein, and the prokaryotic cell contains and expresses a vector comprising the DNA coding for the heterologous protein operably linked to the DNA coding for the signal peptide OmpA.
    Type: Grant
    Filed: November 14, 2001
    Date of Patent: August 8, 2006
    Assignee: Boehringer Ingelheim International GmbH
    Inventors: Jiradej Manosroi, Aranya Manosroi, Chatchai Tayapiwatana, Friedrich Goetz, Rolf-Guenther Werner
  • Promoter sequences for urocortin II and the use thereof
    Patent number: 7087385
    Abstract: Various human urocortin II promoter sequences are disclosed. Nucleic acids and host cells that contain the promoter sequences are also disclosed. Further disclosed are various methods involving the use of these sequences. Also disclosed are methods of modulating the activity of a human urocortin II promoter sequence and methods of modulating urocortin II expression in a cell.
    Type: Grant
    Filed: May 15, 2003
    Date of Patent: August 8, 2006
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Ned H. Kalin, Patrick Henry Roseboom, Steven Anil Nanda