Patents Examined by James Martinell
  • Patent number: 10842822
    Abstract: Disclosed are methods for diagnosing Parkinson's disease, or identifying a risk of developing Parkinson's disease, comprising measuring the amount of a biomolecule in a blood sample, liver sample, or hepatocyte. Also disclosed are methods for preventing or treating Parkinson's disease, comprising administering a therapeutically effective plurality of hepatocytes to a subject in need thereof.
    Type: Grant
    Filed: October 5, 2016
    Date of Patent: November 24, 2020
    Assignee: Orig3n, Inc.
    Inventors: Marcie A. Glicksman, Nikhat F. Zaidi, Robin Y. Smith
  • Patent number: 10829804
    Abstract: The present invention is directed methods for identifying, in a sample, one or more target nucleotide sequences differing from other nucleotide sequences in the sample by one or more nucleotides, one or more copy numbers, one or more transcript sequences, and/or one or more methylated residues, using ligation detection reactions, polymerase mediated extension reactions, and/or cleavage reactions. The present invention is also directed to methods for identifying, in a sample, one or more nucleotides in a target nucleotide sequence.
    Type: Grant
    Filed: March 23, 2016
    Date of Patent: November 10, 2020
    Assignees: The University of North Carolina at Chapel Hill, Cornell University, Board of Supervisors of Louisiana State University and Agricultural and Mechanical College
    Inventors: Francis Barany, John William Efcavitch, Steven A. Soper, Sunggook Park
  • Patent number: 10829821
    Abstract: Disclosed herein are methods and kits for identifying a subject as having leukemia. Also provided herein are methods and kits for determining a leukemia subtype in subject. Further provided herein are methods and kits for determining the prognosis of a subject having leukemia and for determining the progression of leukemia in a subject.
    Type: Grant
    Filed: August 3, 2018
    Date of Patent: November 10, 2020
    Inventors: Kang Zhang, Rui Hou, Lianghong Zheng
  • Patent number: 10821440
    Abstract: A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir superior to the array of wells through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: capturing a population of non-cell particles into the array of wells in single-particle format; releasing, from the non-cell particles, a set of probes into the array of wells; capturing a population of cells into the array of wells in single-cell format; releasing biomolecules from each captured cell into the array of wells; and generating a set of genetic complexes comprising the biomolecules associated with a single captured cell and a subset of probes within individual wells of the array of wells.
    Type: Grant
    Filed: July 10, 2019
    Date of Patent: November 3, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Kalyan Handique, Vishal Sharma
  • Patent number: 10808244
    Abstract: The present disclosure relates to normalization of biological samples, particularly samples comprising nucleic acids to be sequenced. The normalization protocols described herein may be utilized across multiple samples to cap total stoichiometric input and minimize variations in transcript abundance on a per-sample basis in a multiplexed fashion to dramatically increase the accuracy, capacity and efficiency of nucleic acid sequencing.
    Type: Grant
    Filed: March 21, 2019
    Date of Patent: October 20, 2020
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: Thomas Battersby, Adrian Nielsen Fehr, Minxue Zheng
  • Patent number: 10808283
    Abstract: The present invention relates to an in vitro method of identifying a molecular subtype of a tumor in a cancer patient and to a method of stratifying a cancer patient for tumor treatment. The present invention further relates to kits that are useful for identifying a molecular subtype of a tumor in a cancer patient.
    Type: Grant
    Filed: August 19, 2014
    Date of Patent: October 20, 2020
    Inventors: Ralph Wirtz, Christoph Kneip
  • Patent number: 10801067
    Abstract: The present invention relates to methods to detect an amount of DNA that originates from cells of a given type, where the sample comprising such DNA in admixture with DNA that does not originate from such cells. Such methods are based on differential methylation, at certain regions, of the DNA that originates from the given type of cells compared to the admixed DNA. Such methods have particular application in the detection, from a biological fluid from a pregnant female, of cell free DNA that originates from a foetus or the placenta of a foetus, or the detection, from a biological fluid from an individual, of cell free DNA that originates from cells of a tumour.
    Type: Grant
    Filed: May 8, 2015
    Date of Patent: October 13, 2020
    Assignee: Eurofins LifeCodexx GmbH
    Inventors: Sebastian Grömminger, Wera Hofmann, Hamed Said, Matthias Sachse
  • Patent number: 10793911
    Abstract: The invention relates to methods of analysing a sample from a subject having or suspected of having Crohn's disease for the abundance of the subject's nucleic acid (e.g., DNA) in the sample. The invention also relates to methods for measuring abundance of nucleic acid (e.g., DNA) in stool from a human subject having or suspected of having Crohn's Disease (CD). In various embodiments, an in vitro method includes analysing the relative abundance of the host (human) DNA in said sample of stool or nucleic acid extracted or isolated from a stool sample from the host (human); determining the relative abundance of the host (human's) DNA in the sample; and associating the abundance of the host (human) DNA with the host (human) providing the stool sample, or the host (human) providing the stool sample from which the nucleic acid was extracted.
    Type: Grant
    Filed: January 22, 2018
    Date of Patent: October 6, 2020
    Assignee: ENTEROME
    Inventor: Alessandra Cristina L Cervino
  • Patent number: 10787701
    Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.
    Type: Grant
    Filed: May 16, 2019
    Date of Patent: September 29, 2020
    Assignee: Prognosys Biosciences, Inc.
    Inventor: Mark S. Chee
  • Patent number: 10787712
    Abstract: Analysis of 13,023 genes in 11 breast and 11 colorectal cancers revealed that individual tumors accumulate an average of ˜90 mutant genes but that only a subset of these contribute to the neoplastic process. Using stringent criteria to delineate this subset, we identified 189 genes (average of 11 per tumor) that were mutated at significant frequency. The vast majority of these genes were not known to be genetically altered in tumors and are predicted to affect a wide range of cellular functions, including transcription, adhesion, and invasion. These data define the genetic landscape of two human cancer types, provide new targets for diagnostic and therapeutic intervention and monitoring.
    Type: Grant
    Filed: March 19, 2020
    Date of Patent: September 29, 2020
    Assignee: The Johns Hopkins University
    Inventors: Tobias Sjoblom, Sian Jones, D. Williams Parsons, Laura D. Wood, Jimmy Cheng-Ho Lin, Thomas Barber, Diana Mandelker, Bert Vogelstein, Kenneth W. Kinzler, Victor E. Velculescu
  • Patent number: 10781489
    Abstract: Systems and methods to diagnose sarcoidosis are described. In addition to diagnosing sarcoidosis, the systems and methods can distinguish sarcoidosis from tuberculosis. Further disclosed is a cDNA library and methods of its use for reliably identifying sarcoidosis markers.
    Type: Grant
    Filed: March 4, 2016
    Date of Patent: September 22, 2020
    Inventors: Lobelia Samavati, Harvinder S. Talwar, Rita Rosati, Felix Fernandez-Madrid, Jia Li
  • Patent number: 10779518
    Abstract: The present invention relates, inter alia, to methods for determining whether or not an animal and/or its offspring is more likely than not to have an increased tolerance to heat, an increased resistance to ticks, and/or a desirable coat texture. It also provides methods for selecting or rejecting animals, one or more cells or embryos, estimating the worth of an animal, generating animals having a desired genotype/phenotype, cloning and breeding animals and herd formation.
    Type: Grant
    Filed: October 24, 2014
    Date of Patent: September 22, 2020
    Assignee: Livestock Improvement Corporation Limited
    Inventors: Mathew Douglas Littlejohn, Stephen Richard Davis
  • Patent number: 10781473
    Abstract: The present invention provides a plurality of pairs of proximity probes, each pair being capable of binding to a different target analyte, wherein the first and second proximity probes of each pair of probes comprise universal oligonucleotides conjugated to their analyte binding moieties, and hybridised to the universal oligonucleotides are different tag oligonucleotides comprising universal complement domains common to all tag oligonucleotides and unique domains unique to each tag oligonucleotide, as well as methods for their production.
    Type: Grant
    Filed: October 21, 2016
    Date of Patent: September 22, 2020
    Inventors: Johan Erik Simon Fredriksson, Klas Martin Lundberg
  • Patent number: 10774383
    Abstract: High-throughput long read sequencing is used to perform immunogenomic characterization of expressed antibody repertoires in the context of vaccination. Informatic analysis allows global characterizations of isotype distributions, determination of the lineage structure of the repertoire and measure age and antigen related mutational activity. Global analysis of the immune system's clonal structure provides direct insight into the effects of vaccination and provides a detailed molecular portrait of age-related effects.
    Type: Grant
    Filed: June 21, 2018
    Date of Patent: September 15, 2020
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Stephen R. Quake, Joshua Weinstein, Ning Jiang, Daniel S. Fisher
  • Patent number: 10760073
    Abstract: Provided herein are methods, compositions, and kits for assays, many of which involve amplification reactions such as digital PCR or droplet digital PCR. The assays may be used for such applications as sequencing, copy number variation analysis, and others. In some cases, the assays involve subdividing a sample into multiple partitions (e.g., droplets) and merging the partitions with other partitions that comprise adaptors with barcodes.
    Type: Grant
    Filed: December 18, 2018
    Date of Patent: September 1, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Serge Saxonov
  • Patent number: 10738361
    Abstract: Provided are methods and kits for classifying a subject as being more likely to have benign multiple sclerosis (BMS) or as being more likely to have typical relapsing remitting multiple sclerosis (RRMS). Classification of multiple sclerosis disease course is performed by comparing a level of expression of at least one gene involved in the RNA polymerase I pathway in a cell of the subject to a reference expression data of said at least one gene obtained from a cell of at least one subject pre-diagnosed as having BMS and/or from a cell of at least one subject pre-diagnosed as having typical RRMS, thereby classifying the subject as being more likely to have BMS or as being more likely to have typical RRMS. Also provided are methods of diagnosing and treating multiple sclerosis and methods of monitoring treatment efficiency.
    Type: Grant
    Filed: June 19, 2017
    Date of Patent: August 11, 2020
    Assignee: Tel HaShomer Medical Research Infrastructure and Services Ltd.
    Inventors: Anat Achiron, Michael Gurevich
  • Patent number: 10731146
    Abstract: Compositions and methods for nucleic acid isolation from an environmental or biological sample comprising nucleic acid-analysis interferents, particularly from microbiome-containing samples, are provided.
    Type: Grant
    Filed: March 1, 2018
    Date of Patent: August 4, 2020
    Inventors: Alexander Vlassov, Sarah Larocca, Mu Li
  • Patent number: 10732175
    Abstract: The present invention relates generally to compositions, methods and kits for detection of a molecule of interest. The present invention is based, at least in part, on the design of nucleic acid oligonucleotides such that they can be used as molecular building blocks which can be assembled (polymerized) to form a dsDNA polymerization product which can be detected and further, can be disassembled (depolymerized) such that the polymerization product is no longer detectable. The invention can be performed multiple times on the same cell population, and is therefore useful for highly sensitive in situ detection of multiple biomarkers.
    Type: Grant
    Filed: December 14, 2016
    Date of Patent: August 4, 2020
    Assignee: The Penn State Research Foundation
    Inventors: Yong Wang, Erin Gaddes, Jinping Lai, Niancao Chen
  • Patent number: 10724077
    Abstract: The present invention relates to self-contained integrated systems and methods for constructing nucleic acid profiles of human individuals and for identification of the human individuals.
    Type: Grant
    Filed: February 5, 2016
    Date of Patent: July 28, 2020
    Assignee: Laboratory Corporation of America Holdings
    Inventors: Nani M. Grimmer, Michael Neal Parsons, Abigail Bathrick, Donia Palomo Slack
  • Patent number: 10724100
    Abstract: Systems and methods for mitigating prostate cancer development are provided. Peripheral blood cells may be evaluated for the presence or quantity of gamma-H2AX foci, and/or for gene alterations encoding a protein with impaired or lack of function, for example, because the encoded protein is truncated, and correlating with prostate cancer development. Such nucleic acids may encode proteins from or peripheral to the DNA damage repair pathway and/or androgen receptor signaling pathway, or that are otherwise correlated with prostate cancer development. Such genes include one or more of AKR1C1, PALB2, APTX, BLM, BRCA1, CTBP1, DDB2, FANCA, FANCL, MBD5, MSH3, NEIL3, RAD51D, RAD54L2, SP1, TP53BP1, UBE2D3, UBE2V2, NRIP1, EFCAB6, CRISP3, PAPSS2, ATP6V0A2, ALG13, MGAT2, B3GAT3, DOLK, FLT3, ASXL1, KDR, or NOTCH2.
    Type: Grant
    Filed: September 16, 2015
    Date of Patent: July 28, 2020
    Assignee: Institute For Cancer Research
    Inventors: Sanjeevani Arora, Greg Enders, Erica Golemis, Emmanuelle Nicolas, Ilya Serebriiskii