Abstract: DNA expression vectors capable, in a transformant strain of yeast, of expressing a polypeptide under the control of a genetically distinct yeast promoter, processes of forming transformant strains of yeast and transformed yeast strains are disclosed.
Type:
Grant
Filed:
June 6, 1995
Date of Patent:
January 5, 1999
Assignees:
Washington Research Foundation, Genentech, Inc.
Inventors:
Ronald A. Hitzeman, Franklin E. Hagie IV, Benjamin D. Hall, Gustav Ammerer
Abstract: A recombinant DNA cloning vector useful for expressing exogenous protein is described, which comprises(a) a DNA segment containing a functional origin of replication;(b) one or more DNA segments, each of which conveys to a transformable host cell a property useful for selection when said vector is transformed into said host cell; and(c) a DNA segment comprising a sequence that defines, in tandem,(1) the promoter of a lipoprotein expression control sequence,(2) the 5' untranslated region of a lipoprotein expression control sequence and(3) a translation start codon followed, without interposition of a portion or all of a nucleotide sequence coding for endogenous protein, by a nucleotide sequence coding for an enterokinase cleavage site to which is joined, without interruption, a nucleotide sequence coding for an exogenous protein.
Type:
Grant
Filed:
March 6, 1984
Date of Patent:
May 17, 1988
Assignee:
Eli Lilly and Company
Inventors:
Nancy G. Mayne, J. Paul Burnett, Ramamoorthy Belegaje, Hansen M. Hsiung
Abstract: The present transcription terminators are DNA sequences characterized by their ability to terminate the transcription of DNA sequences. The present sequences are portable, have a variety of restriction endonuclease sites and are useful in controlling the expression in hosts of DNA sequences coding for prokaryotic, eukaryotic and viral proteins and polypeptides.
Type:
Grant
Filed:
September 27, 1984
Date of Patent:
December 1, 1987
Assignee:
Eli Lilly and Company
Inventors:
Ramamoorthy Belagaje, Stuart A. Kuhstoss, R. Nagaraja Rao
Abstract: Recombinant vector plasmids containing a DNA fragment coding for human interferon-.beta. inserted downstream from a tryptophan promoter are useful for transformation of microorganisms such as Escherichia coli which transformants produce human interferon-.beta..
Type:
Grant
Filed:
December 22, 1982
Date of Patent:
August 11, 1987
Assignees:
Hakko Kogyo Co., Ltd. Kyowa, Juridical Foundation, Japanese Foundation for Cancer Research
Abstract: This invention relates to a process for sulfur removal from gas streams by contacting an active culture of photosynthetic sulfur bacteria with reductive sulfur compounds and carbon oxides in the gas stream under substantially anaerobic conditions with irradiation by electromagnetic energy and cultivating the bacteria to form elemental sulfur and organic carbon compounds. The process is particularly suited for removal of hydrogen sulfide from acid-gas streams and from natural or raw pipeline gas with greater than 95 percent and usually greater than about 98 percent sulfur removal from gas streams. The process is operable with gas streams of very low hydrogen sulfide content of about 0.1 and less Mole percent and gas streams of high hydrogen sulfide content. The process utilizes Chlorobium bacteria to produce elemental sulfur and organic carbon compounds which may be advantageously utilized to produce hydrocarbon fuels.