Abstract: The present invention relates to a method of subgrouping media formulations into stable, compatible components that can then be solubilized at high concentrations (10.times. to 100.times.). Concentrated solutions of the ingredient containing subgroups can be used to prepare media formulations. Concentrated culture media formulations (2-10.times.) or 1.times. cell culture media can be prepared according to the present invention by mixing a sufficient amount of the concentrated subgroup solutions with a sufficient amount of a diluent (water, buffer, etc.). The present invention further relates to stabilization of glutamine by complexing or mixing glutamine with divalent metal cations in solution.