Abstract: Rapid electrochemical verification of the amplification of DNA by a polymerase chain reaction in a small sample of the PCR product.

Abstract: The present invention relates to the detection of specific nucleic acid sequences, either by a process of amplification of specific nucleic acid sequences or not. More particularly the invention provides for improved compositions and methods for reducing the chance for contamination from manipulation of reagents, internal controls for amplification, and the use of automated apparatus for the automated detection of one, or more than one amplified nucleic acid sequences.

Abstract: Cells are genetically modified to express a luminophore, e.g., a modified (F64L, S65T, Y66H) Green Flourescent Protein (GFP, EGFP) coupled to a component of an intracellular signalling pathway such as a transcription factor, a cGMP- or cAMP-dependent protein kinase, a cyclin-, calmodulin- or phospholipid-dependent or mitogen-activated serine/threonin protein kinase, a tryosine protein kinase, or a protein phosphatase (e.g. PKA, PKC, Erk, Smad, VASP, actin, p38, Jnkl, PKG, IkappaB, CDK2, Grk5, Zap70, p85, protein-tyrosine phosphatase 1C, Stat5, NFAT, NFkappaB, RhoA, PKB). An influence modulates the intracellular signaling pathway in such a way that the luminophore is being redistributed or translocated with the component in living cells in a manner experimentally determined to be correlated to the degree of influence.

Abstract: The present invention is directed to a transcription based amplification method for the amplification of DNA targets. With the method of the present invention an isothermal transcription based amplification method is provided for the amplification of double stranded DNA.

Abstract: The invention comprises a multi-color, comparative hybridization assay method using an array of nucleic acid target elements attached to a solid support for the simultaneous detection of both gene expression and chromosomal abnormalities in a tissue sample. The method of the invention employs a comparative hybridization of a tissue mRNA or cDNA sample labeled in a first fluorescent color, a tissue chromosomal DNA sample labeled in a second fluorescent color, and at least one reference nucleic acid labeled in a third fluorescent color, to the array. The fluorescent color presence and intensity at each of at least two target elements are detected and the fluorescent ratios (i) of the first and third colors and (ii) the second and third colors determined. Gene expression and chromosomal abnormalities are thus simultaneously detected.

Abstract: This invention relates to devices, methods, and compositions of matter for the multiplex amplification and analysis of nucleic acid sequences in a sample using ligation-dependent strand displacement amplification technologies in combination with bioelectronic microchip technology.

Abstract: A method for detecting the homozygous or heterozygous state of mutations assumed to be present in a nucleic acid by simultaneously using two primer pairs. The two different primer pairs lead to the production of amplified fragments of different sizes, and the number and quality of the amplified bands enables homozygous and heterozygous items to be distinguished on the basis of said mutation.

Abstract: The present invention provides a metal charged iminodiacetic acid (IDA) cellulose for detecting a test sample having a histidine tag. The present invention also provides methods for determining cloned protein expression and function. Additionally, the present invention includes a method for the handling of denatured proteins with subsequent renaturation in situ (parenthetically after binding to metal charged IDA cellulose). A wide range of applications are contemplated for the metal charged IDA cellulose including two-dimensional high throughput screening of proteins.

Abstract: A thermal cycling device comprising a ceramic sample plate, and method of use thereof, is provided. The device is adapted for in situ thermal processing of biological samples on planar substrates, in particular amplification of nucleic acids. The device comprises electrical heating means attached to the ceramic sample plate, and forced air cooling means.

Abstract: Circular RNAs may be synthesized by inserting DNA fragments into a plasmid containing sequences having the capability of spontaneous cleavage and self-circularization. Insertion of the DNA fragments allows RNAs of predetermined size to be constructed. In addition, a two-dimensional polyacrylamide gel electrophoresis system having a second dimension more highly cross-linked than the first dimension permits the separation and analysis as well as the precise sizing of both linear and circular RNAs produced by the synthetic method.

Abstract: The present invention provides a computer method and apparatus for detecting mutations in DNA fragments. Specifically, percent acetonitrile and threshold temperature, for use in the denaturing high performance liquid chromatography method of separating DNA fragments, are determined. In a preferred embodiment, the present invention serves as a preprocessor to the DHPLC system described in the cited art.

Abstract: The present invention provides a fast, simple and specific method for generating a complete full-length cDNA library from single cells. The first reverse transcription of intracellular mRNAs with an oligo(dT)n-promoter primer introduces a recognition site for following transcription of newly reverse-transcribed cDNAs. The poly-nucleotide tailing of above cDNAs in addition to aforementioned promoter region further forms binding templates for specific PCR amplification. After repeating the reverse transcription, transcription, reverse transcription and PCR procedure, we can multiply a single copy of mRNA to two billion folds by calculation based upon the comparison between the amount of a synthesized cDNA library and that of theoretically presumed mRNAs within a cell (0.1 pg). In conjunction with a cell fixation and permeabilization step, the complete full-length cDNA library can be directly generated from few single cells without mRNA degradation.

Abstract: A method of assay of a specific nucleic acid anticipated in a sample, which comprises:a DNA producing step which involves production of a double-stranded DNA having a promoter sequence for an RNA polymerase and the nucleic sequence of the specific nucleic acid (the specific nucleic acid sequence) downstream from the promoter sequence by using the specific nucleic acid in the sample as a template, andan RNA producing and measuring step which involves production of a single-stranded RNA having the specific nucleic acid sequence by the RNA polymerase and measurement of the single-stranded RNA,wherein the RNA producing and measuring step is initiated by adding at least the RNA polymerase, ribonucleoside triphosphates and a probe which is labeled with a fluorescent intercalative dye and is complementary to the single-stranded RNA to the reaction solution after the DNA producing step, involves measurement of the fluorescence intensity of the reaction solution, and is carried out at a constant temperature, and does

Abstract: Continuous amplification reaction provide a method of amplifying a specific nucleic acid without the need to cycle a reaction. The method produces RNA transcripts which can be detected by a variety of methods. Amplification and detection kits are also provided.

Abstract: An apparatus for the automated synthesis of molecular arrays. A jetting device is employed along with a reaction chamber to dispense reagents used in the synthesis onto the substrate. A positioning system moves the substrate from the jet to the reaction chamber. A controller controls the movement of the substrate and the application of the reagents so that the synthesis is carried out according to a pre-determined procedure. The apparatus will synthesize nucleotides in an array of micron-size spots according to a pattern selected by the operator immediately prior to synthesis.

Abstract: A method for quantifying total mRNA in a biological sample containing RNA such as crude cell lysates containing cytosolic mRNA, which method comprises the steps of: (a) incubating the sample with an oligo-(dT)- or poly-U-immobilized microtiter plate; (b) washing non-hybridized components from the microtiter plate; (c) labeling the hybridized mRNA with a photometric nucleic-acid dye; (d) measuring the amount of label captured on the microtiter plate; (e) heat-denaturing the labeled mRNA; (f) washing the denatured mRNA from the microtiter plate; and (g) measuring the amount of label remaining on the microtiter plate; and (h) correlating the amount of the measured label (captured label minus remaining label) with the quantity of total mRNA present in the sample, thereby easily measuring the total mRNA without the influence of rRNA or tRNA and without radioactive dyes, which method can be adapted to chemosensitivity tests.