Abstract: The present invention relates to methods and compositions for the inhibition of gene expression. In particular, the present invention provides oligonucleotide-based therapeutics for the inhibition of oncogenes involved in cancers.
Type:
Grant
Filed:
June 1, 2004
Date of Patent:
April 28, 2009
Inventors:
Reza Sheikhnejad, Mina Patel Sooch, Neal Goodwin, David Olson
Abstract: Antisense compounds, compositions and methods are provided for modulating the expression of apolipoprotein B. The compositions comprise antisense compounds, particularly antisense oligonucleotides, targeted to nucleic acids encoding apolipoprotein B. Methods of using these compounds for modulation of apolipoprotein B expression and for treatment of diseases associated with expression of apolipoprotein B are provided.
Type:
Grant
Filed:
November 13, 2003
Date of Patent:
March 31, 2009
Assignee:
Genzyme Corporation
Inventors:
Roseanne M. Crooke, Mark Graham, Susan M. Freier
Abstract: A method for the treatment for hepatitis delta infection in a host, that includes administering an effective amount of a nucleoside or a nucleoside analog that suppresses the expression of the hepatitis B surface or preS1 antigen in the host 100-fold or more relative to pretreatment values in vivo; or to not more than 1 microgram per milliliter in vivo. In a preferred embodiment, the nucleoside is L-FMAU, or a pharmaceutically acceptable salt or prodrug thereof.
Type:
Grant
Filed:
November 22, 2002
Date of Patent:
March 31, 2009
Assignees:
Georgetown University, University of Georgia Research Foundation, Inc.
Inventors:
John L. Casey, Brent E. Korba, Paul J. Cote, John L. Gerin, Bud C. Tennant, Chung K. Chu
Abstract: Briefly described, embodiments of this disclosure include bioluminescence resonance energy transfer (BRET) systems, methods of detecting a protein-protein interaction, noninvasive methods for detecting the interaction of a first protein with a second protein within a living animal, methods to determine the efficacy of a test compound administered to modulate the interaction of a first protein with a second protein in a living animal, BRET vectors, kits relating to each of the above, transgenic cell or progeny thereof and/or animals relating to each of the above, and the like.
Abstract: The present invention relates to methods and compositions for the inhibition of gene expression. In particular, the present invention provides oligonucleotide-based therapeutics for the inhibition of oncogenes involved in cancers.
Type:
Grant
Filed:
June 1, 2004
Date of Patent:
March 3, 2009
Assignee:
ProNAI Therapeutics, Inc.
Inventors:
Gholamreza Sheikhnehjad, Mina Patel Sooch, Neal Goodwin, David Olson
Abstract: Compounds comprising an oligonucleotide moiety covalently linked to a lipid moiety are disclosed. The oligonucleotide moiety comprises a sequence that is complementary to the RNA component of human telomerase. The compounds inhibit telomerase activity in cells with a high potency and have superior cellular uptake characteristics.
Abstract: A DNA sequence encoding a protein capable of binding to a tumor necrosis factor receptor-associated factor (TRAF) molecule, TRAF-binding proteins, their isoforms, analogs, fragments and derivatives encoded by the DNA sequence, their methods for the production of the DNA sequences and proteins, and the uses for the DNA sequence and proteins.
Type:
Grant
Filed:
April 1, 1997
Date of Patent:
February 3, 2009
Assignee:
Yeda Research and Development Co. Ltd.
Inventors:
David Wallach, Nikolai Malinin, Mark Boldin, Andrei Kovalenko, Igor Mett
Abstract: Regulatory elements of the 5?UTR of cold-shock inducible genes that prolong the expression of cold-shock inducible genes under conditions that elicit the cold-shock response in a bacterium, repress the expression of the cold-shock inducible genes under physiological conditions, or enhance the translation of cold-shock inducible genes under conditions that elicit a cold-shock response in bacteria; vectors incorporating these elements; and methods for expressing proteins.
Type:
Grant
Filed:
April 19, 2000
Date of Patent:
September 30, 2008
Assignee:
University of Medicine and Dentistry of New Jersey
Abstract: The present invention relates to a porcine uroplakin II gene promoter, an expression vector containing the promoter, and a method for producing useful proteins using the vector. The promoter of the present invention promotes the bladder-specific expression of a target protein at high efficiency. An animal, which was transformed using the inventive promoter so as to express the target protein, secretes the target protein in its urine at high concentration, and the protein thus produced shows a superior physiological activity to that of the same kind of the existing protein. As a result, the inventive promoter, the expression vector and transgenic animal using the promoter, can be advantageously used in the production field of useful proteins that are medicinally valuable.
Abstract: Compositions and methods for the treatment of asthma with oligonucleotides which specifically hybridize with nucleic acids encoding B7 proteins.
Type:
Grant
Filed:
August 15, 2003
Date of Patent:
June 26, 2007
Assignee:
ISIS Pharmaceuticals, Inc.
Inventors:
C. Frank Bennett, Timothy A. Vickers, James G. Karras, Susan M. Freier
Abstract: Nucleic acid sequences containing unmethylated CpG dinucleotides that modulate an immune response including stimulating a Th1 pattern of immune activation, cytokine production, NK lytic activity, and B cell proliferation are disclosed. The sequences are also useful a synthetic adjuvant.
Abstract: The present invention provides prophylactic and therapeutic methods of treating the ductal epithelium of an exocrine gland, in particular a mammary gland, for disease, in particular cancer. The methods comprise contacting the ductal epithelium of the exocrine gland with an epithelium-destroying gent, preferably by ductal cannulation, so as to realize a prophylactic or therapeutic effect.
Type:
Grant
Filed:
July 12, 2005
Date of Patent:
March 27, 2007
Assignee:
Johns Hopkins University School Of Medicine
Abstract: A method for transfecting T cells with a nucleic acid molecule comprising a gene such that the gene is expressed in the T cells is described. The T cells are stimulated and proliferating prior to introduction of the nucleic acid molecule.
Type:
Grant
Filed:
April 20, 2004
Date of Patent:
February 6, 2007
Assignees:
The United States of America as represented by the Secretary of the Navy, The Regents of the University of Michigan
Inventors:
Carl H. June, Thompson B. Craig, Suil Kim
Abstract: The present invention provides a class of Conformationally Assisted Probes comprising (a) a nucleic acid moiety; (b) an energy donor moiety; (c) an energy acceptor moiety; and (d) one or more stabilizing moieties.
Abstract: The present invention provides a pharmaceutical composition, comprising: (a) cationic lipids, wherein said lipids are a liposomal mixture of a diacyl-ethyl-phosphocholine and 1,2-diacyl-sn-glycero-3-phosphoethanolamine; and (b) a plasmid cDNA sequence encoding a protein having tumor suppressor or pro-apoptotic activity. This composition has a high gene transfection efficiency at non-toxic doses and is designed to transfect human bronchial premalignant lesions and early endo-bronchial malignancies.
Abstract: Novel tamoxifen inducible and ICI 182,780 repressible expression systems comprising mutant estrogen receptors and mutant estrogen response element are disclosed. Such systems have a wide variety of applications, including gene therapy and in vivo and in vitro expression, as well as their use in transgenic animals.
Type:
Grant
Filed:
March 11, 2002
Date of Patent:
December 26, 2006
Assignee:
The Board of Trustees of the University of Illinois
Abstract: Compositions and methods are provided for modulating the expression of bcl-x. Antisense compounds, particularly antisense oligonucleotides, targeted to nucleic acids encoding bcl-x are preferred. Methods of using these compounds for modulation of bcl-x expression and for treatment of diseases associated with expression of bcl-x are also provided. Methods of sensitizing cells to apoptotic stimuli are also provided.
Type:
Grant
Filed:
November 21, 2002
Date of Patent:
December 12, 2006
Assignee:
Isis Pharmaceuticala, Inc.
Inventors:
C. Frank Bennett, Nicholas M. Dean, Brett P. Monia, Brian J. Nickoloff, Vivian Q. Zhang
Abstract: A strategy for suppressing specifically or partially specifically an endogenous gene and introducing a replacement gene, said strategy comprising the steps of: 1. providing suppressing nucleic acids or other suppression effectors able to bind to an endogenous gene, gene transcript or gene product to be suppressed and 2. providing genomic DNA or cDNA (complete or partial) encoding a replacement gene wherein the suppressing nucleic acids are unable to bind to equivalent regions in the genomic DNA or cDNA to prevent expression of the replacement gene. The replacement nucleic acids have modifications in one or more third base (wobble) positions such that replacement nucleic acids still code for the wild type or equivalent amino acids.
Type:
Grant
Filed:
April 2, 1997
Date of Patent:
November 21, 2006
Assignee:
Optigen Patents Limited
Inventors:
Gwenyth Jane Farrar, Peter Humphries, Paul Francis Kenna
Abstract: Preparations of conjugates of a receptor-binding internalized ligand and a cytocide-encoding agent and compositions containing such preparations are provided. The conjugates contain a polypeptide that is reactive with an FGF receptor, such as bFGF, or another heparin-binding growth factor, cytokine, or growth factor coupled to a nucleic acid binding domain. One or more linkers may be used in the conjugation. The linker is selected to increase the specificity, toxicity, solubility, serum stability, or intracellular availability, and promote nucleic acid condensation of the targeted moiety. The conjugates are complexed with a cytocide-encoding agent, such as DNA encoding saporin. Conjugates of a receptor-binding internalized ligand to a nucleic acid molecule are also provided.
Type:
Grant
Filed:
July 2, 2002
Date of Patent:
November 21, 2006
Assignee:
Prizm Pharmaceuticals, Inc.
Inventors:
J. Andrew Baird, Lois Ann Chandler, Barbara A. Sosnowski
Abstract: In one aspect, the invention provides a method for separating a mixture of polynucleotides, such as DNA or RNA, including (a) applying the mixture to a polymeric separation medium having non-polar surfaces, wherein the surfaces are characterized by being substantially free from multivalent cations, such as metal ions, which are free to interfere with polynucleotide separation, and (b) eluting the mixture with a mobile phase containing organic solvent and counter ion agent. In the separation of single-stranded polynucleotides, improved separation is obtained at a temperature effective to fully denature secondary structure within the polynucleotides.
Type:
Grant
Filed:
November 16, 2000
Date of Patent:
November 21, 2006
Assignee:
Transgenomic, Inc.
Inventors:
Douglas T. Gjerde, Robert M. Haefele, Paul D. Taylor, Christopher P. Hanna, Alezander I. Kuklin, David P. Hornby