Patents Examined by Janet L. Epps
  • Polynucleotides encoding rodent antibodies with human idiotypes and animals comprising same
    Patent number: 10385132
    Abstract: The invention relates to polynucleotides, particularly chimeric polynucleotides useful for optimal production of functional immunoglobulins with human idiotypes in rodents. The invention further relates to rodents comprising such polynucleotides.
    Type: Grant
    Filed: October 24, 2016
    Date of Patent: August 20, 2019
    Assignee: OPEN MONOCLONAL TECHNOLOGY, INC.
    Inventors: Marianne Bruggemann, Roland Buelow, Michael J. Osborn, Biao Ma
  • Optimized in vivo delivery system with endosomolytic agents for nucleic acid conjugates
    Patent number: 10363317
    Abstract: The present invention relates to an optimized in vivo delivery system with endosomolytic agents for nucleic acid of therapeutic interest conjugated to molecules facilitating endocytosis, in particular for use in the treatment of cancer.
    Type: Grant
    Filed: June 26, 2017
    Date of Patent: July 30, 2019
    Assignees: ONXEO, INSTITUT CURIE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
    Inventors: Jian-Sheng Sun, Marie Dutreix, Maria Quanz
  • Lipid nanoparticles for transfection and related methods
    Patent number: 10342760
    Abstract: Transfection reagent composition, lipid nanoparticles prepared from the transfection reagent composition, kits that include the transfection reagent composition, and methods for making and using lipid nanoparticles prepared from the transfection reagent composition. Lipids when dispersed in aqueous media readily form liposomes, such as unilamellar vesicles and multilamellar vesicles. Liposomes have been used successfully to encapsulate and deliver a wide range of chemicals including nucleic acids, proteins and small molecule drugs, to cells.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: July 9, 2019
    Assignee: THE UNIVERSITY OF BRITISH COLUMBIA
    Inventors: Euan Ramsay, R. James Taylor, Colin Walsh, Nathan M. Belliveau, Pieter R. Cullis, Timothy Leaver, Andre Wild
  • Enhanced placental stem cells and uses thereof
    Patent number: 10329529
    Abstract: Provided herein are placental stem cells that exhibit increased survival (“enhanced placental stem cells”), compositions comprising such placental stem cells, and methods of using such placental stem cells and compositions.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: June 25, 2019
    Assignee: CELULARITY, INC.
    Inventors: Stewart Abbot, Kathy Karasiewicz-Mendez, Xiaokui Zhang
  • Controlled gene expression methods
    Patent number: 10329333
    Abstract: Methods and compositions for controlling gene expression are disclosed.
    Type: Grant
    Filed: October 17, 2017
    Date of Patent: June 25, 2019
    Assignee: The Children's Hospital of Philadelphia
    Inventors: Gerd Blobel, Wulan Deng
  • Polyethyleneimine polyethyleneglycol vectors
    Patent number: 10278991
    Abstract: A polyplex of a double stranded RNA and a polymeric conjugate is provided, wherein the polymeric conjugate consists of a linear polyethyleneimine covalently linked to one or more polyethylene glycol (PEG) moieties, each PEG moiety being conjugated via a linker to a targeting moiety capable of binding to a cancer antigen.
    Type: Grant
    Filed: May 14, 2015
    Date of Patent: May 7, 2019
    Assignee: TARGIMMUNE THERAPEUTICS AG
    Inventors: Alex Levitzki, Salim Joubran, Alexei Shir, Maya Zigler, Alaa Talhami, Yael Langut
  • Induced dendritic cells and uses thereof
    Patent number: 10272111
    Abstract: The present invention relates to cells engineered to express at least one cytokine and at least one antigen which induces the self differentiation of dendritic cell (DC) progenitor cells into functional antigen-presenting induced DC (iDC). Moreover, therapeutic uses of said iDC for regenerating the immune system after transplantation of hematopoietic stem cells are disclosed. Said iDC are also useful for generating mice with a functional endogenously regenerated humanized immune system producing antigen-specific T and B cell responses which can be used as animal models for the study of the human adaptive immune responses.
    Type: Grant
    Filed: August 7, 2015
    Date of Patent: April 30, 2019
    Assignee: Medizinische Hochschule Hannover
    Inventors: Renata Stripecke, Gustavo Salguero-Lopez, Anusara Daenthanasanmak, Arnold Ganser
  • Method and device for cell modification
    Patent number: 10273504
    Abstract: The invention relates to a cell modification device, comprising a centrifugation chamber with at least one cell modifying surface with a normal vector having an angle of 135?45° to the rotational axis of the centrifugation chamber, wherein the centrifugation chamber comprises at least one input/output port and the cells to be modified are immobilized at the cell modifying surfaces by the rotation of the centrifugation chamber at 2 to 2000 g.
    Type: Grant
    Filed: April 13, 2017
    Date of Patent: April 30, 2019
    Assignee: Miltenyi Biotec, GmbH
    Inventors: Stefan Miltenyi, Alexander Scheffold
  • Nanoparticles comprising aminoacyl tRNA synthetase and anticancer composition comprising same
    Patent number: 10258674
    Abstract: The present invention relates to nanoparticles comprising aminoacyl tRNA synthetase and an anticancer composition comprising the same and, specifically, to nanoparticles which comprise glycyl-tRNA synthetase (GRS), leucyl-tRNA synthetase (LRS), and isoleucyl-tRNA synthetase (IRS), and have anticancer or immunostimulating activity; a pharmaceutical composition for preventing or treating cancer, comprising the nanoparticles as an active ingredient; a composition for immunostimulation; and a method for preparing the nanoparticles.
    Type: Grant
    Filed: November 28, 2016
    Date of Patent: April 16, 2019
    Assignee: MEDICINAL BIOCONVERGENCE RESEARCH CENTER
    Inventors: Sunghoon Kim, Min Chul Park
  • Methods and compositions for using argonaute to modify a single stranded target nucleic acid
    Patent number: 10253311
    Abstract: The present disclosure provides compositions, kits, genetically modified cells, non-human transgenic organisms, and methods for binding and/or cleaving a single stranded target nucleic acid. A method of cleaving includes contacting a single stranded target nucleic acid with (e.g., introducing into a cell) a subject argonaute (Ago) polypeptide and a guide RNA (e.g., having a 5?-OH). In some embodiments, a subject Ago polypeptide includes an amino acid sequence having 70% or more sequence identity with amino acids 282-430 and/or 431-639 of the Marinitoga piezophila argonaute (MpAgo) protein set forth in SEQ ID NO: 1. The present disclosure provides variant Ago polypeptides; and methods of use of same.
    Type: Grant
    Filed: April 9, 2015
    Date of Patent: April 9, 2019
    Assignee: The Regents of the University of California
    Inventors: Jennifer A. Doudna, Emine Kaya, Kilian R. Knoll
  • Upconversion of light for use in optogenetic methods
    Patent number: 10252076
    Abstract: Provided herein are compositions comprising lanthanide-doped nanoparticles which upconvert electromagnetic radiation from infrared or near infrared wavelengths into the visible light spectrum. Also provided herein are methods activating light-responsive opsin proteins expressed on plasma membranes of neurons and selectively altering the membrane polarization state of the neurons using the light delivered by the lanthanide-doped nanoparticles.
    Type: Grant
    Filed: July 19, 2016
    Date of Patent: April 9, 2019
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Karl Deisseroth, Polina Anikeeva
  • Induced pluripotent stem cell selection method and method for inducing differentiation to blood cells
    Patent number: 10240126
    Abstract: A method for producing hematopoietic stem cells and/or hematopoietic progenitor cells from pluripotent stem cells is described. The method includes a step of culturing pluripotent stem cells in the presence of IGF2. A method is described for selecting an induced pluripotent stem cell(s) having high capacity to differentiate into hematopoietic stem cells and/or hematopoietic progenitor cells, or into blood cells, based on the expression level(s) of one or more genes such as TRIM58, CTSF, FAM19A5, and TCERG1L genes, or on the DNA methylation state(s) of the TRIM58, CSMD1, and/or FAM19A5 gene(s).
    Type: Grant
    Filed: June 11, 2014
    Date of Patent: March 26, 2019
    Assignee: KYOTO UNIVERSITY
    Inventors: Shinya Yamanaka, Yoshinori Yoshida, Masatoshi Nishizawa
  • Multi-functional particles and methods of using the same
    Patent number: 10232050
    Abstract: Provided herein are multi-functional particles. The particles may include poly(lactide-co-glycolide)-g-polyethylenimine (PLGA-g-PEI (PgP)), at least one targeting moiety, at least one therapeutic agent, and/or at least one nucleic acid. Also provided herein are methods of using the multi-functional particles.
    Type: Grant
    Filed: December 11, 2015
    Date of Patent: March 19, 2019
    Assignee: Clemson University
    Inventor: Jeoung Soo Lee
  • Method for inducing cardiac differentiation of pluripotent stem cell with low-molecular compounds
    Patent number: 10233426
    Abstract: The present invention provides a method for inducing cardiac differentiation of a pluripotent stem cell, which comprises the steps of (1) culturing a pluripotent stem cell in a medium containing a WNT signaling activator and a PKC activator and (2) culturing the cell after the step (1) in a medium containing a WNT signaling inhibitor, a Src inhibitor, and an EGFR inhibitor.
    Type: Grant
    Filed: May 29, 2015
    Date of Patent: March 19, 2019
    Assignee: KYOTO UNIVERSITY
    Inventors: Norio Nakatsuji, Itsunari Minami
  • Use of liposomes in a carrier comprising a continuous hydrophobic phase for delivery of polynucleotides in vivo
    Patent number: 10232052
    Abstract: The invention provides compositions comprising a carrier comprising a continuous phase of a hydrophobic substance, liposomes, and a polynucleotide, and methods for using such compositions for delivering a polynucleotide to a subject.
    Type: Grant
    Filed: October 11, 2016
    Date of Patent: March 19, 2019
    Assignee: IMMUNOVACCINE TECHNOLOGIES INC.
    Inventors: Marc Mansour, Mohan Karkada, Genevieve Mary Weir
  • DNA vectors, transposons and transposases for eukaryotic genome modification
    Patent number: 10233454
    Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
    Type: Grant
    Filed: October 6, 2016
    Date of Patent: March 19, 2019
    Assignee: DNA2.0, INC.
    Inventors: Jeremy Minshull, Mark Welch, Sridhar Govindrajan, Maggie Lee, Kate Caves, Jon Ness
  • Bone matrix compositions having nanoscale textured surfaces
    Patent number: 10220115
    Abstract: Bone matrix compositions having nanoscale textured surfaces and methods for their production are provided. In some embodiments, bone matrix is prepared for implantation and retains nanoscale textured surfaces. In other embodiments, nanostructures are imparted to bone matrix wherein collagen fibrils on the surface of the bone matrix have been compromised, thus imparting a nanoscale textured surface to the bone matrix. Generally, these methods may be applied to mineralized or demineralized bone including partially or surface demineralized bone.
    Type: Grant
    Filed: January 27, 2017
    Date of Patent: March 5, 2019
    Assignee: Warsaw Orthopedic, Inc.
    Inventors: Guobao Wei, Keyvan Behnam, Nanette Forsyth
  • Liquefaction of bone matrix
    Patent number: 10220116
    Abstract: The invention relates to methods of preparing a bone matrix solution, a bone matrix implant, and variants thereof. The invention also relates to methods of cell culture using the same. The invention further relates to bone matrix scaffolds comprising one or more bone matrix nanofibers, methods of preparing, and methods of use thereof. The invention also relates to methods of culturing cells and promoting differentiation of stem cells using the same.
    Type: Grant
    Filed: August 16, 2017
    Date of Patent: March 5, 2019
    Assignee: Lifenet Health
    Inventors: Michael Francis, Roy Ogle
  • Lipids and lipid nanoparticle formulations for delivery of nucleic acids
    Patent number: 10221127
    Abstract: Compounds are provided having the following structure: or a pharmaceutically acceptable salt, tautomer or stereoisomer thereof, wherein R1a, R1b, R2a, R2b, R3a, R3b, R4a, R4b, R5, R6, R7, R8, R9, L1, L2, G1, G2, G3, a, b, c and d are as defined herein. Use of the compounds as a component of lipid nanoparticle formulations for delivery of a therapeutic agent, compositions comprising the compounds and methods for their use and preparation are also provided.
    Type: Grant
    Filed: June 29, 2016
    Date of Patent: March 5, 2019
    Assignee: Acuitas Therapeutics, Inc.
    Inventors: Xinyao Du, Steven M. Ansell
  • Alkenyl substituted 2,5-piperazinediones, compositions, and uses thereof
    Patent number: 10201618
    Abstract: Provided herein are compounds of Formula (I), and salts thereof, wherein each instance of RL is independently optionally substituted C6-C40 alkenyl. Further provided are compositions comprising a compound of Formula (I) and an agent. Further provided are methods and kits using the compositions for delivering an agent to a subject or cell and for treating and/or preventing a range of diseases. Further provided are methods of preparing compounds of Formula (I) and precursors thereof.
    Type: Grant
    Filed: June 17, 2016
    Date of Patent: February 12, 2019
    Assignee: Massachusetts Institute of Technology
    Inventors: Daniel Griffith Anderson, Joseph R. Dorkin, Owen Shea Fenton, Kevin John Kauffman, Rebecca L. McClellan