Abstract: The present invention relates to fusion polynucleotides for biosynthesis of beta-carotene and a method for producing beta-carotene using the same. More particularly, it relates to fusion polynucleotides encoding phytoene synthase, connective sequences of FMDV-derived 2A sequence or internal ribosome entry site (IRES) and carotene desaturase, and a method for producing beta-carotenes using the same. Fusion polynucleotides and the recombinant vector using the same of the present invention have the effect of expression both phytoene synthase gene and carotene desaturase gene stably within cell transformants. Accordingly, fusion polynucleotides of the present invention can be used to regulate the biosynthetic metabolism of plant producing beta-carotene. Furthermore, it can be applied to effectively increase the content of beta-carotene, a useful metabolite.
Type:
Grant
Filed:
August 29, 2008
Date of Patent:
October 15, 2013
Assignee:
Republic of Korea, Rural Development Administration
Inventors:
Sun Hwa Ha, Ju Kon Kim, Ha Rin Jung, Jung Bong Kim, Young Mi Kim, Seok-Cheol Suh, Liang Ying Shi, Soon Jong Kweon, Dong Hern Kim