Patents Examined by Jean Caraballo-Leon
  • Patent number: 11498070
    Abstract: Microfluidic devices having a construct formed from perfluoropolyether and poly(ethylene glycol) diacrylate. The construct includes an inlet for receiving a continuous phase fluid, an inlet for receiving a dispersed phase fluid, and a plurality of channels extending through the construct. The plurality of channels are in fluid communication with both the inlet of the continuous phase fluid and the inlet of the dispersed phase fluid. The construct further includes a plurality of microdroplet generators configured to produce microdroplets, each of the microdroplet generators in fluid communication with the plurality of channels. Additionally, the construct includes an outlet formed in the construct and in fluid connection with the plurality of microdroplet generators.
    Type: Grant
    Filed: March 21, 2019
    Date of Patent: November 15, 2022
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Daeyeon Lee, David Issadore, Sagar Prasad Yadavali, Heon-Ho Jeong
  • Patent number: 11491481
    Abstract: A diagnostic tester for analysing a body fluid includes a test tape having test zones configured to receive the body fluid. The tester further includes a chamber configured to contain the test tape and a seal. The tester further includes an opening of the chamber which is at least partially bordered by the seal. The tester further includes a face on the chamber that borders the opening, at least part of the face being formed by the seal. The tester further includes a closing leaf spring sealed onto the face, the closing leaf spring shielding the opening from an environment outside the chamber. The tester further includes an exit gap formed between the closing leaf spring and the seal through which the test tape is configured to exit the chamber.
    Type: Grant
    Filed: June 2, 2018
    Date of Patent: November 8, 2022
    Inventor: Brandon Bransgrove
  • Patent number: 11471883
    Abstract: A fluid chip suitable for a fluid device is disclosed in which an upper surface of a flow passage has another member bonded thereto. The disclosed fluid chip, in which a flow passage is formed, comprises a base material having a top surface forming at least a portion of a bottom surface of a flow passage, and a bonding member which is formed from an elastomer resin and an upper end surface of which is provided in a position higher than the top surface of the base material. The base material has a support post portion which projects from the top surface and defines the height of a side surface of the flow passage, and the support post portion of the base material is embedded in the bonding member.
    Type: Grant
    Filed: November 9, 2018
    Date of Patent: October 18, 2022
    Assignee: Enplas Corporation
    Inventors: Takumi Yamauchi, Nobuya Sunaga
  • Patent number: 11453005
    Abstract: Various embodiments comprise systems, methods, architectures, mechanisms or apparatus configured to separate particles of varying size within a fluid flow, or filter particles from a fluid flow, via an array of anchored-liquid drops or anchored-gas drops.
    Type: Grant
    Filed: May 25, 2018
    Date of Patent: September 27, 2022
    Inventors: German Drazer, Shahab Shojaei-Zadeh
  • Patent number: 11453008
    Abstract: A device for sorting bio-particles by image-manipulated electric force includes a first substrate, a second substrate, a fluidic channel, one or more photosensitive layers and an inlet hole. The first substrate has a first conductive electrode, and the second substrate has a second conductive electrode. The second conductive electrode is disposed opposite the first conductive electrode. The fluidic channel is disposed between the first conductive electrode and the second conductive electrode. The photosensitive layer is conformally disposed on at least one of the surfaces of the first conductive electrode and the second conductive electrode. The inlet hole is disposed in the first conductive electrode and the first substrate, where the inlet hole includes a first opening close to the fluidic channel and a second opening away from the fluidic channel, and the surface area of the first opening is greater than the surface area of the second opening.
    Type: Grant
    Filed: March 18, 2020
    Date of Patent: September 27, 2022
    Assignee: Ace Medical Technology Co., Ltd.
    Inventors: Tzu-Keng Chiu, Yu-Xian Zhu, Huan-Yi Tseng
  • Patent number: 11446662
    Abstract: A system for capturing a biomolecule in a single cell includes: a two-dimensional array having single cell capture holes on one surface of a substrate, and biomolecule capture areas inside the substrate each comprising a biomolecule capture member for capturing a biomolecule extracted from individual cells respectively captured by the single cell capture holes; a flow channel for flowing a sample containing cells to be assayed, from a 1st direction parallel to the surface of the substrate; a structure on the surface of the substrate and opposed to the 1st direction on the downstream side of each of the single cell capture holes; a 1st application means applying a 1st flow, and a 1st control means; and a 2nd application means applying a 2nd flow orthogonal to the one surface of the substrate toward each biomolecule capture area from each corresponding single cell capture hole, and a 2nd control means.
    Type: Grant
    Filed: March 28, 2016
    Date of Patent: September 20, 2022
    Assignee: HITACHI, LTD.
    Inventors: Tomoyuki Sakai, Masataka Shirai
  • Patent number: 11440009
    Abstract: A method may include etching a number of holes into a carrier wafer layer to form a plurality of filters in the carrier wafer layer, patterning a chamber layer over a first side of the carrier wafer layer to form chambers above each filter formed in the carrier wafer layer, forming a layer over the chamber layer, grinding a second side of the carrier wafer layer to expose the number of holes etched into the carrier wafer layer, and bonding a molded substrate to the carrier wafer layer opposite the chamber layer.
    Type: Grant
    Filed: July 15, 2016
    Date of Patent: September 13, 2022
    Assignee: Hewlett-Packard Development Company, L.P.
    Inventors: Chien-Hua Chen, Michael W. Cumbie, Devin Alexander Mourey
  • Patent number: 11396014
    Abstract: A microfluidic movement control method utilizing light, a device, and a microtubule actuator (2). The microtubule actuator (2) is prepared by utilizing a light-induced deformed smart polymer material. The smart polymer material forms, by an exciting beam, asymmetrical deformation, and is induced to produce a capillary action to drive a microfluid movement. The embodiment can drive microfluids having various polarities and compositions, and can drive creep of the microfluid, and can even drive the microfluid to generate a 3D movement trail. The embodiment has found a wide range of potential applications in controllable microfluidic transport, micro-reaction systems, micro-mechanic systems, IC laboratories, and others.
    Type: Grant
    Filed: February 1, 2019
    Date of Patent: July 26, 2022
    Inventors: Yanlei Yu, Jiuan Lv, Jia Wei, Yuyun Liu
  • Patent number: 11378571
    Abstract: Embodiments of the present disclosure include methods and systems for detecting the presence of endotoxins in filters. For example, embodiments of the present disclosure are directed to methods and systems for improving in-process controls by detecting undesirable quantities of endotoxins in filters containing naturally-sourced materials prior to using those filters in the production of formulated drug substances.
    Type: Grant
    Filed: April 26, 2019
    Date of Patent: July 5, 2022
    Assignee: Regeneron Pharmaceuticals, Inc.
    Inventors: Nathan Mao, Annamarie Cruz, Bernhard Schilling, Scott Carver
  • Patent number: 11371982
    Abstract: The purpose of the present invention is to provide a method for accurately predicting a cancer patient prognosis based on a count of desired cells for which expression of a leukocyte marker and an epithelial marker is hardly exhibited by detecting those cells. Provided is a method for diagnosing an overall survival prognosis for a patient suffering from cancer, the method including: a step of obtaining a concentrated solution containing desired cells by pre-treating a biological sample obtained from the patient; a step of optically detecting the concentrated cells; and a step of detecting the desired cells from the detected image, wherein an association is made with the overall survival prognosis diagnosis by counting the detected desired cells, and wherein the desired cells are cells confirmed by the existence of a cell nucleus and in which expression of a leukocyte marker and an epithelial marker is hardly exhibited.
    Type: Grant
    Filed: January 19, 2017
    Date of Patent: June 28, 2022
    Inventors: Yasuyuki Akiyama, Atsushi Morimoto, Toru Futami, Fumiaki Koizumi, Takeshi Sawada
  • Patent number: 11340214
    Abstract: Among others, the present invention provides apparatus for detecting a disease, comprising a system delivery biological subject and a probing and detecting device, wherein the probing and detecting device includes a first micro-device and a first substrate supporting the first micro-device, the first micro-device contacts a biologic material to be detected and is capable of measuring at the microscopic level an electric, magnetic, electromagnetic, thermal, optical, acoustical, biological, chemical, physical, or mechanical property of the biologic material.
    Type: Grant
    Filed: November 14, 2018
    Date of Patent: May 24, 2022
    Assignee: AnPac Bio-Medical Science Co., Ltd.
    Inventors: Chris C. Yu, He Yu, Xuedong Du
  • Patent number: 11340244
    Abstract: A method and apparatus for removing magnetic beads together with molecular targets of interest from a fluid is described. A consumable bead collection probe is placed in a vessel containing magnetic beads suspended in the fluid. A magnet outside the vessel concentrates magnetic beads on a surface of the collection probe so they may be manipulated in and out of vessels during washing or processing steps without transferring the fluid into or out of a vessel. In a further embodiment, a vibrational source is attached to the collection probe such that, in combination with the magnet in a position external to the vessel, beads may be washed without releasing them from the collection probe.
    Type: Grant
    Filed: January 28, 2020
    Date of Patent: May 24, 2022
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventor: Hannah Pezzi
  • Patent number: 11331668
    Abstract: The invention relates to a method of performing an acoustophoretic operation, comprising the steps of: a. providing an acoustophoretic chip comprising a polymer substrate in which a microfluidic flow channel is positioned, b. providing at least one ultrasound transducer in acoustic contact with one surface of the substrate, c. actuating the at least one ultrasound transducer at a frequency f that corresponds to an acoustic resonance peak of the substrate including the microfluidic flow channel filled with a liquid suspension, and d. providing the liquid suspension in the flow channel to perform the acoustophoretic operation on the liquid suspension. The invention further relates to an acoustophoretic device, a method of producing an acoustophoretic device, and a microfluidic system comprising the acoustophoretic device.
    Type: Grant
    Filed: August 31, 2018
    Date of Patent: May 17, 2022
    Assignee: ACOUSORT AB
    Inventors: Henrik Bruus, Rayisa Moiseyenko, Torsten Freltoft, Pelle Ohlsson, Ola Jakobsson
  • Patent number: 11331665
    Abstract: A microfluidic centrifuge is provided that includes a microfluidic disc having a pair of through holes that include a first through hole and a second through hole, where the pair of through holes are disposed symmetric and proximal to a central axis of the microfluidics disc, where the microfluidics disc includes a sealable input port and a sealable output port, where the sealable input port and the sealable output port are connected by a microfluidics channel, and a pair of tethers disposed through the pair of through holes that are disposed to twist about each other for unwinding where the tether unwinding is disposed to interact with the through holes, where the microfluidics disc rotates about the central axis.
    Type: Grant
    Filed: January 9, 2017
    Date of Patent: May 17, 2022
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Manu Prakash, Mohammed Saad Bhamla, James Stanley Cybulski, Chew Chai, Aanchal Johri, Brandon Benson
  • Patent number: 11280766
    Abstract: This invention relates to encapsulated workflow reagents comprising an encapsulating material and a workflow reagent encapsulated within the encapsulating material for sample and workflow preparation prior to chromatographic, spectroscopic or other analytical systems, use thereof, and devices comprising the same.
    Type: Grant
    Filed: July 28, 2017
    Date of Patent: March 22, 2022
    Assignee: Waters Technologies Corporation
    Inventors: Kevin Daniel Wyndham, Darryl W. Brousmiche
  • Patent number: 11241688
    Abstract: A microfluidic system includes a microfluidic chip having a plurality of fluid channels, each fluid channel having an opening providing access to an interior of the fluid channel, and a gasket disposable on the microfluidic chip in an aligned configuration, the gasket including a first side configured to face the microfluidic chip in the aligned configuration, a second side opposite the first side, and an aperture extending through the gasket from the first side to the second side, the aperture being sized and positioned to allow a communication of pressure from the second side of the gasket to the openings of at least two fluid channels when the gasket is in the aligned configuration.
    Type: Grant
    Filed: December 5, 2019
    Date of Patent: February 8, 2022
    Assignee: 10x Genomics, Inc.
    Inventors: Tobias Daniel Wheeler, Rajiv Bharadwaj
  • Patent number: 11169141
    Abstract: The invention relates to a method of detecting a clot in a measurement chamber of a liquid sample analyzer, wherein the liquid sample analyzer comprises at least two analyte sensors, a first analyte sensor, for measuring a first analyte in a liquid sample, and one or more second analyte sensors, for measuring one or more second analytes in the liquid sample in the measurement chamber, the method comprising the steps of, (a) at least partly filling the measurement chamber with a known solution having a composition comprising the first analyte at a pre-determined level, and the second one or more analytes at pre-determined levels, (b) obtaining a first sequence of measurement results by the first analyte sensor, and simultaneously obtaining a second sequence of measurement results by the second, one or more analyte sensors, (c) determining a change of the first sequence of measurement results, (d) determining a change of the second one or more sequence of measurement results, (e) comparing the change of the fir
    Type: Grant
    Filed: December 19, 2016
    Date of Patent: November 9, 2021
    Assignee: Radiometer Medical ApS
    Inventors: Michael Taagaard, Peter Aage Frischauf, Flemming Aas
  • Patent number: 11131608
    Abstract: A target analyte is extracted out of a sample fluid in a sample fluid passage by diffusing the target analyte through a supported liquid membrane to a product fluid passage. Extraction of the target analyte is accelerated by applying an electric field across and perpendicular to the supported liquid membrane with electrodes. Passage of selected ions across an exchange membrane extending between one of the electrodes and the supported liquid membrane is inhibited.
    Type: Grant
    Filed: October 23, 2015
    Date of Patent: September 28, 2021
    Assignee: Hewlett-Packard Development Company, L.P.
    Inventors: Viktor Shkolnikov, Anita Rogacs
  • Patent number: 11073522
    Abstract: The structures of very long chain dicarboxylic acids from blood samples may be validated through chemical derivatization of the dicarboxylic functionality with 2-picolylamine before positive ESI mass spectroscopy analysis. Laboratory standards may be used to quantify the concentration of the selected very long chain dicarboxylic acid in a blood sample.
    Type: Grant
    Filed: May 3, 2018
    Date of Patent: July 27, 2021
    Assignee: Lincoln Memorial University
    Inventor: Paul L. Wood
  • Patent number: 11067576
    Abstract: A method for monitoring health maintenance by collecting an initial blood sample from an individual and dividing the collected blood sample into at least two parts. The first of the two parts is immediately analyzed for multiple blood components levels, and the second part is frozen immediately after the collection to be used at a later time as a comparative standard. After a period of time, a second blood sample is collected from the same individual and analyzed for the same multiple blood components levels in a parallel test with the frozen part. The results obtained from the second blood sample are then compared to the results from the frozen comparative standard to detect real changes in the multiple blood components levels over time.
    Type: Grant
    Filed: March 9, 2018
    Date of Patent: July 20, 2021
    Inventor: Martin King