Patents Examined by Jeffery R. Snay
  • Thiobenzoylation method of peptide sequencing with gas chromatography and mass spectrometric detection
    Patent number: 5246865
    Abstract: A protein microsequencing method for use in conjunction with the thiobenzoylation degradation of polypeptides and proteins is disclosed. The process involves reaction of the N-terminal amino acid of a polypeptide with an excess of a thiobenzoylating reagent. The derivatized polypeptide is subjected to cleavage by acid which forms a 4-substituted 2-phenyl-5(4H) thiazolone. The thiazolone is acylated to form a 5-acyloxy-2-phenylthiazole and subjected to detection by both gas chromatography and chemical ionization mass spectroscopy.
    Type: Grant
    Filed: July 24, 1992
    Date of Patent: September 21, 1993
    Assignee: California Institute of Technology
    Inventor: Mark L. Stolowitz