Patents Examined by Jeffrey Fredman
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Patent number: 7273697Abstract: A method for identifying an unknown base sequence present in a target single-stranded nucleic acid utilizing a probe array in which single-stranded nucleic acid probes are arranged as isolated spots on a substrate, where each probe has a base sequence complementary to one of the plural base sequences expected to be the unknown base sequence, and a fluorescence pattern of a sample on the probe array is compared with template patterns to identify the base sequence of the sample.Type: GrantFiled: August 31, 2001Date of Patent: September 25, 2007Assignee: Canon Kabushiki KaishaInventors: Nobuko Yamamoto, Tadashi Okamoto, Tomohiro Suzuki
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Patent number: 7273702Abstract: A nucleic acid refining apparatus, being ease in automation thereof, keeping high in contacting frequency between nucleic acid within a sample and a solid phase during nucleic acid capture processing, thereby proving high capturing rate, comprises: means for separating a liquid containing the nucleic acid therein from said sample through centrifugal force; means for transferring a reagent through the centrifugal force; means for producing a mixture liquid of said reagent transferred through the centrifugal force and a solution containing said nucleic acid therein; a carrier for capturing said nucleic acid; means for transferring said mixture liquid to said carrier through the centrifugal force; heating means for heating said carrier; and a holding means for separating and holding the reagent containing said nucleic acid eluting from said carrier, separating from other reagent, through different centrifugal.Type: GrantFiled: May 24, 2002Date of Patent: September 25, 2007Assignee: Hitachi, Ltd.Inventors: Teruhisa Akashi, Yukiko Ikeda, Yoshihiro Nagaoka, Naruo Watanabe, Yuji Miyahara
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Patent number: 7267984Abstract: A method of assembling large DNA fragments in a chromosome using site specific recombinases and alternating excisionases. The method may be performed in vitro or in vivo, but larger assemblies are possible when the assembly is performed in vivo. For an in vivo assembly, the cell must be engineered to contain the desired recombinases, each in an inducible construct so that the desired recombinase can be expressed at the correct time with the correct choice of inducing agent.Type: GrantFiled: October 31, 2003Date of Patent: September 11, 2007Assignee: Rice UniversityInventor: George Nelson Bennett
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Patent number: 7262010Abstract: Ralstonia solanacearum, the causal agent of bacterial brown rot of potato, is often carried latently in seed potato tubers. Primers and a probe were designed for a real-time BIO-PCR assay technique for detecting potato tubers latently infected with R. Solanacearum. Using naturally infected potato tubers, as few as 20 cells/ml extract could be detected. Two of 14 naturally infected potato tubers with no disease symptoms were positive by the newly described real-time BIO-PCR (pre-enrichment on agar or in liquid medium) assay but not by direct real-time PCR.Type: GrantFiled: October 30, 2002Date of Patent: August 28, 2007Assignee: The United States of America, as represented by the Secretary of AgricultureInventors: Norman W. Schaad, Phillip E. Gaush, Meric Ozakman
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Patent number: 7262290Abstract: A mammalian KIR 4.1 gene and gene products which are predictive of a susceptibility or predisposition to neurological disorders such as multiple epilepsy phenotypes are provided. Methods of predicting an individual's susceptibility in developing or having a neurological disorder via detection of these diagnostic markers are also provided. In addition, compositions and methods for identifying compositions for use in the treatment of neurological disorders via these genes and gene products are described.Type: GrantFiled: August 24, 2001Date of Patent: August 28, 2007Assignee: Trustees of the University of PennsylvaniaInventor: Wade H. Berrettini
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Patent number: 7262031Abstract: Disclosed herein is a method for synthesizing a desired nucleic acid sequence. The method comprises dividing the desired sequence into a plurality of partially overlapping segments; optimizing the melting temperatures of the overlapping regions of each segment to disfavor hybridization to the overlapping segments which are non-adjacent in the desired sequence; allowing the overlapping regions of single stranded segments which are adjacent to one another in the desired sequence to hybridize to one another under conditions which disfavor hybridization of non-adjacent segments; and filling in, ligating, or repairing the gaps between the overlapping regions, thereby forming a double-stranded DNA with the desired sequence. Also disclosed is a method for preventing errors in the synthesis of the nucleic acid sequence.Type: GrantFiled: May 21, 2004Date of Patent: August 28, 2007Assignee: The Regents of the University of CaliforniaInventors: Richard H. Lathrop, G. Wesley Hatfield
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Patent number: 7255989Abstract: The invention concerns a method for preparing nucleic acids from an environment sample, more particularly a method for obtaining a library of nucleic acids from a sample. The invention also concerns nucleic acids of nucleic acid libraries obtained by said method their use in the synthesis of novel compounds, in particular novel compounds of therapeutic interest. The invent further concerns novel means used in the method for obtaining said nucleic acids, such as novel vectors and novel processes for preparing such vectors or recombinant host cells containing said nucleic acid. Finally, the invention concerns methods for detecting a nucleic acid of interest within a library of nucleic acids resulting from said method, and nucleic acids detected by said method and polypeptides encoded by said nucleic acids.Type: GrantFiled: November 27, 2000Date of Patent: August 14, 2007Assignee: Aventis Pharma S.A.Inventors: Pascale Jeannin, Jean-Luc Pernodet, Michel Guerineau, Pascal Simonet, Sophie Courtois, Camela Cappellano, François Francou, Alain Raynal, Maria Ball, Guennadi Sezonov, Karine Tuphile, Asa Frostegard
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Patent number: 7255992Abstract: The present invention provides methods for rapid detection of bioagents for environmental and product testing. The methods can be used for testing air, water, soil, surfaces of buildings, containers, towers and the like, as well as testing of foodstuff and cosmetics.Type: GrantFiled: September 12, 2003Date of Patent: August 14, 2007Assignee: Isis Pharmaceuticals, IncInventors: David J. Ecker, Richard H. Griffey, Rangarajan Sampath, Steven A. Hofstadler, John McNeil, Stanley T. Crooke, Lawrence B. Blyn, Thomas A. Hall
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Patent number: 7252935Abstract: The present invention provides methods and kits useful for detecting neplasia by measuring the methylation level of biomarkers, especially the promoter region of GSTP1 for the detection of prostate adenocarcinoma.Type: GrantFiled: November 15, 2002Date of Patent: August 7, 2007Assignee: The John Hopkins University School of MedicineInventor: David Sidransky
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Patent number: 7247454Abstract: The present invention provides improved methods for the production of recombinant peptides from bacterial cells.Type: GrantFiled: December 20, 2002Date of Patent: July 24, 2007Assignee: XOMA Technology Ltd.Inventors: Marc D. Better, Patrick D. Gavit
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Patent number: 7244567Abstract: This invention relates to methods of sequencing DNA. More specifically, this invention relates to methods of sequencing both the sense and antisense strands of DNA through the use of blocked and unblocked sequencing primers. In brief, these methods include the steps of annealing an unblocked primer to a first strand of nucleic acid; annealing a second blocked primer to a second strand of nucleic acid; elongating the nucleic acid along the first strand with a polymerase; terminating the first sequencing primer; deblocking the second primer; and elongating the nucleic acid along the second strand.Type: GrantFiled: January 28, 2004Date of Patent: July 17, 2007Assignee: 454 Life Sciences CorporationInventors: Yi-Ju Chen, John H. Leamon, Kenton L. Lohman, Michael T. Ronan, Jonathan M. Rothberg, Maithreyan Srinivasan, Michael P. Weiner
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Patent number: 7241598Abstract: A method for preparing an antigen-specific antibody by constructing a library of phage-displayed single chain variable fragment of an antibody with a novel frame-shifting PCR is disclosed. Also disclosed is a method for preparing a clone for producing an antigen-specific antibody.Type: GrantFiled: June 29, 2004Date of Patent: July 10, 2007Assignee: The Chinese University of Hong KongInventors: Wing-Tai Cheung, Man Cheng
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Patent number: 7241567Abstract: A herbicide resistant transformed sugar beet that is detectable by the specific primers developed to match the DNA sequences that flank the left and/or right border region of the inserted transgenic DNA and the method of identifying primer pairs containing plant genomic DNA/plasmid DNA. More specifically the present invention covers a specific glyphosate resistant sugar beet plant having an insertion of the transgenic material identified as the T227-1 event. The present invention additionally covers primer pairs: plant genomic DNA/Plasmid DNA that are herein identified. Additionally, these primer pairs for either the left or the right flanking regions make an event specific test for the T227-1 insert of transgenic material.Type: GrantFiled: November 30, 2001Date of Patent: July 10, 2007Assignee: SES Europe N.V./S.A.Inventors: Guy Weyens, Steve Barnes, Inge Rosquin
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Patent number: 7241572Abstract: A method for isolating and purifying nucleic acids with an improved recovery yield is provided. A mixed solution containing the nucleic acids, salts, and an organic solvent is contacted with an adsorption support to cause the nucleic acids to be adsorbed on the support. Then, the nucleic acids are desorbed from the support using an elution bugger. At least one compound containing 2 to 10 carbon atoms as selected from the group consisting of aliphatic ether, aliphatic ester, and aliphatic ketone is used as the organic solvent. The method improves the yield of nucleic acids collection, is easy to implement and less susceptible to contamination.Type: GrantFiled: March 2, 2004Date of Patent: July 10, 2007Assignee: Hitachi, Ltd.Inventors: Kyoko Kojima, Satoshi Ozawa
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Patent number: 7238484Abstract: Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread.Type: GrantFiled: December 17, 2004Date of Patent: July 3, 2007Assignee: The Regents of the University of CaliforniaInventors: Daniel J. Pinkel, Joe W. Gray, Anne Kallioniemi, Olli-Pekka Kallioniemi, Frederic Waldman
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Patent number: 7238477Abstract: The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, nucleotides are covalently attached to Raman labels before incorporation into a nucleic acid. In other embodiments, unlabeled nucleic acids are used. Exonuclease treatment of the nucleic acid results in the release of labeled or unlabeled nucleotides that are detected by Raman spectroscopy. In alternative embodiments of the invention, nucleotides released from a nucleic acid by exonuclease treatment are covalently cross-linked to nanoparticles and detected by surface enhanced Raman spectroscopy (SERS), surface enhanced resonance Raman spectroscopy (SERRS) and/or coherent anti-Stokes Raman spectroscopy (CARS). Other embodiments of the invention concern apparatus for nucleic acid sequencing.Type: GrantFiled: September 12, 2003Date of Patent: July 3, 2007Assignee: Intel CorporationInventors: Xing Su, Andrew A. Berlin, Selena Chan, Steven J. Kirch, Tac-Woong Koo, Gabi Neubauer, Valluri Rao, Narayanan Sundararajan, Mineo Yamakawa
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Patent number: 7235387Abstract: The present invention relates to a method for mutation analysis of the HIV pol gene of HIV virions comprising amplifying virion RNA or DNA via nested PCR using outer primers as represented in SEQ ID No. 1 and 2, amplifying said PCR product via nested PCR using a 5? and 3? primer chosen from the inner primers SEQ ID No. 3, 4, 5, and 6, and sequencing this secondary obtained PCR product using at least one sequencing primer chosen from any of SEQ ID No. 7 to 12 or variants thereof. In the alternative, at least one secondary sequencing primer may be used chosen from any of SEQ ID No. 13 to 24. The benefit of the sequences present in the invention resides in the fact that, with the aid of the oligonucleotides, the sequences of all presently known HIV subtypes and all mutations of the pol gene presently known to yield resistance towards antiretroviral therapy can be determined. The present invention also relates to kits for performing such a method as well as primers for performing the same.Type: GrantFiled: April 20, 2001Date of Patent: June 26, 2007Assignee: Virco BVBAInventors: Brendan Larder, Sharon Kemp, Stuart Bloor, Ann Brophy
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Patent number: 7232654Abstract: This invention is in the field of lymphadenopathy virus, which has been designated Human Immunodeficiency Virus Type 1 (HIV-1) to detect the presence of DNA, RNA or antibodies of the lymphadenopathy retrovirus associated with the acquired immune deficiency syndrome or of the lymphadenopathy syndrome by the use of DNA fragments or the peptides encoded by said DNA fragments. The invention further relates tot he DNA fragments, vectors comprising them and the proteins expressed.Type: GrantFiled: September 19, 1994Date of Patent: June 19, 2007Assignees: Institut Pasteur, The United States of America as represented by the Secretary of the Department of Health and Human ServicesInventors: Jean-Claude Chermann, Solange Chamaret, Claudine Axler-Blin, Francoise Rey, Marie-Therese Nugeyre, Jacques Gruest, legal representative, Charles Dauguet, Willy Rozenbaum, Christine Rouzioux, Francois Brun-Vezinet, Luc Montagnier, Francoise Barre-Sinoussi, Pierre Tiollais, Marc Alizon, Pierre Sonigo, Simon Wain-Hobson, Stewart Cole, Oliver Danos, Robert C. Gallo, Mikulas Popovic, Mangalasseril G. Sarngadharan, Jacqueline Gruest, deceased
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Patent number: 7232657Abstract: The invention relates to methods of detecting a drug resistant HIV in a subject. The methods include detecting mutations associated with drug resistance in an HIV 2-LTR circle DNA molecule obtained from a cell of an HIV-positive subject, e.g., an HIV-1-positive human.Type: GrantFiled: September 2, 2004Date of Patent: June 19, 2007Assignee: University of MassachusettsInventors: Mario Stevenson, Mark Sharkey
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Patent number: 7229800Abstract: The present invention relates to nucleic acid amplification assays for the detection of nucleic acid sequences of Neisseria gonorrhoeae. The present invention provides oligonucleotides that are complementary or that anneal to nucleic acid sequences of Neisseria gonorrhoeae. The present invention also provides internal amplification controls (IACs) that can be used in nucleic acid amplification reactions.Type: GrantFiled: April 16, 2004Date of Patent: June 12, 2007Assignee: Becton, Dickinson and CompanyInventor: James M. Harris