Patents Examined by Jeremy Jay
  • Patent number: 4818681
    Abstract: A process for synthesizing an oligonucleotide comprising linking a nucleoside phosphate to a solid support, through the heterocyclic moiety of the nucleoside, coupling a mono- or oligonucleotide to the nucleoside phosphate through its phosphate moiety, in at least one step enzymatically lengthening the mono- or oligonucleotide, cleaving the resultant oligonucleotide from the solid support-nucleoside phosphate at the phosphate moiety of the nucleoside, and separating the oligonucleotide. After cleaving and separating the solid support-nucleoside phosphate is recycled for further coupling. Advantageously the solid support-nucleoside phosphate is phosphorylated between separation and recycling.
    Type: Grant
    Filed: February 22, 1985
    Date of Patent: April 4, 1989
    Assignee: Molecular Diagnostics, Inc.
    Inventor: Nanibhushan Dattagupta
  • Patent number: 4721669
    Abstract: This invention concerns a coordination complex and salts and optically resolved enantiomers thereof, of the formula (R).sub.3 --M, wherein R comprises 1,10-phenanthroline or a substituted derivative thereof, M comprises a suitable transition metal, e.g. ruthenium(II) or cobalt(III), and R is bonded by M by a coordination bond.The complexes of this invention are useful in methods for labeling, nicking and cleaving DNA. The lambda enantiomer of complexes of this invention is useful in methods for specifically labeling, detecting, nicking and cleaving Z-DNA.The complexes may also be used in a method for killing tumor cells and may be combined with a pharmaceutically acceptable carrier to form a pharmaceutical composition for the treatment of tumor cells in a subject. The invention further concerns methods for treating a subject afflicted with tumor cells.
    Type: Grant
    Filed: January 18, 1985
    Date of Patent: January 26, 1988
    Assignee: The Trustees of Columbia University in the City of New York
    Inventor: Jacqueline K. Barton
  • Patent number: 4699876
    Abstract: Nonradiometric polynucleotide probe compositions comprising zymogen-activating polypeptide reporter moieties are disclosed, in addition to detection methods and systems employing said probes.
    Type: Grant
    Filed: January 27, 1984
    Date of Patent: October 13, 1987
    Assignee: E. I. Du Pont de Nemours and Company
    Inventor: Irwin C. Libeskind
  • Patent number: 4689295
    Abstract: Method of detecting the presence of Salmonella in a food sample including providing at least one DNA probe which is capable of selectively hybridizing to Salmonella DNA to form detectable complexes, contacting the DNA probes with the bacteria in the food sample under conditions which allow the probe to hybridize to Salmonella DNA present in the food sample to form hybrid DNA complexes, and detecting the hybrid DNA complexes as an indication of the presence of Salmonella in the food sample.
    Type: Grant
    Filed: September 2, 1983
    Date of Patent: August 25, 1987
    Assignee: Integrated Genetics, Inc.
    Inventors: Robert L. Taber, Renee A. Fitts
  • Patent number: 4689294
    Abstract: The rate of hybridization between two complementary polynucleotide segments in an aqueous medium is increased by the presence of the anionic polymers polyacrylate and polymethacrylate. The acceleration effect is particularly useful in nucleic acid hybridization assays involving immobilization of sample nucleic acids and the use of labeled probes. Nonspecific binding of probe to nitrocellulose supports is substantially lower in the presence of the present polymers than in the presence of the prior art accelerator dextran sulfate. Polyacrylate is particularly advantageous since it has been found to be effective at low concentrations and is significantly less expensive than the prior art compound.
    Type: Grant
    Filed: November 19, 1984
    Date of Patent: August 25, 1987
    Assignee: Miles Laboratories, Inc.
    Inventors: Sophie J. Boguslawski, Leslie H. DeRiemer Anderson
  • Patent number: 4675285
    Abstract: A method for identifying and isolating clones containing DNA coding for a desired protein is described. DNA prepared from a cell that expresses the desired protein is inserted into an isolation expression vector having means for replication (as a means of producing DNA) and a suitable promoter for expression of said DNA in a predetermined mammalian host cell as well as means for replication in a bacterial cell. The transient expression vector is then inserted into a bacterial cell for replication of the DNA. Pools of DNA, prepared from a predetermined number of bacterial clones so that the nucleic acids (DNA and RNA) is substantially free of other bacterial contaminants are transfected or microinjected into mammalian host cells and conditioned medium from growing such cells is tested for the presence of the desired protein. Positive pools are selected and the clones used to make the pool are screened to identify and isolate the clone containing the desired DNA.
    Type: Grant
    Filed: September 19, 1984
    Date of Patent: June 23, 1987
    Assignee: Genetics Institute, Inc.
    Inventors: Steven C. Clark, Randal J. Kaufman, Gordon G. Wong
  • Patent number: 4670380
    Abstract: In a method for determining a particular polynucleotide sequence in a test medium containing single stranded nucleic acids wherein the sample is subjected to a hybridization reaction with a labeled detection probe having a substantially complementary polynucleotide sequence, and wherein after hybridization the label in said probe is assayed, the improvement wherein the label in said labeled probe comprises a fluorescent nucleotide which is linked by a phosphate ester linkage to said probe. Probes and kits therefor are also provided.
    Type: Grant
    Filed: May 23, 1984
    Date of Patent: June 2, 1987
    Assignee: Molecular Diagnostics, Inc.
    Inventor: Nanibhushan Dattagupta
  • Patent number: 4666862
    Abstract: Dyes are provided comprising phycobiliproteins having covalently coupled thereto a plurality of small molecular weight organic dyes under conditions permitting energy transfer mechanisms to operate therebetween. The resultant label may then be used in virtually any immunoassay format.
    Type: Grant
    Filed: August 14, 1984
    Date of Patent: May 19, 1987
    Assignee: Ortho Diagnostic Systems Inc.
    Inventor: Joseph L. W. Chan
  • Patent number: 4666845
    Abstract: Mouse monoclonal antibodies to several cell antigens of human ovarian, cervical and endometrial carcinomas have been produced and characterized. The distribution of the antigens was determined by mixed hemagglutination assays on 153 normal and malignant cell cultures of various types, and by immunoperoxidase staining of frozen sections of 27 normal adult and 24 fetal tissues. five monoclonal antibodies representative of five classes of mAb raised to restricted ovarian, cervical and endometrial cells were tested extensively producing mAb reactive with cancer but not normal cells. One such mAb, MF116 was readily detected in the spent culture medium of metabolically radiolabeled cells. These antibodies, reacting with relatively restricted cell surface antigens, are useful in the analysis of epithelial cell differentiation, in cancer diagnosis and therapy and in tissue typing of normal or abnormal cells.
    Type: Grant
    Filed: December 16, 1983
    Date of Patent: May 19, 1987
    Assignee: Sloan-Kettering Institute
    Inventors: M. Jules Mattes, John L. Lewis, Jr., Kenneth O. Lloyd, Lloyd J. Old, Carlos Cordon-Cardo
  • Patent number: 4666853
    Abstract: Self-sufficient incubation assembly for the in vitro cultivation of microorganisms such as bacteria and for being energized by a self-contained energy source, including a heater for heating means, such as a culture growth dish assembly or a cuvette, for receiving a culture growth medium seeded with microorganisms, to a physiological temperature to cultivate the microorganisms, and electrical circuitry which interconnects the heater with the energy source and which includes a temperature control element in intimate physical contact with the seeded culture growth receiving means to cause the temperature of the control element to be substantially the same as the temperature of the medium; the electrical circuit in operation produces heat and due to its intimate physical contact with the means for receiving the seeded culture growth medium supplements the heating of the medium by the heater and the supplementation reduces the total energy required to be supplied by the energy source to cultivate the microorganism
    Type: Grant
    Filed: July 8, 1983
    Date of Patent: May 19, 1987
    Assignee: Personal Diagnostics, Inc.
    Inventors: Peter M. Meserol, Jesse L. Acker, Janet G. Murnick, Dean Pappas
  • Patent number: 4666828
    Abstract: A method for detecting the presence of the gene for Huntington's disease in a subject which comprises analyzing the human chromosome 4 of the subject for a DNA polymorphism, preferably a restriction fragment length polymorphism (RFLP), linked to Huntington's Disease.
    Type: Grant
    Filed: August 15, 1984
    Date of Patent: May 19, 1987
    Assignee: The General Hospital Corporation
    Inventor: James F. Gusella
  • Patent number: 4664911
    Abstract: Compositions and methods for potentiating the cytotoxic activity of immunogotoxin conjugates are provided. The compositions of the present invention include a selective binding agent such as an antibody coupled to a toxin B chain moiety such as ricin B chain.
    Type: Grant
    Filed: June 21, 1983
    Date of Patent: May 12, 1987
    Assignee: Board of Regents, University of Texas System
    Inventors: Jonathan W. Uhr, Ellen S. Vitetta
  • Patent number: 4661458
    Abstract: A device is described which provides a totally enclosed and secure environment for the culture of cells and for the production of biologicals, pharmaceuticals, and other cell-derived products of commercial value. The device comprises several modules of differing functions: regulating the cellular growth environment, providing a suitable cell growth substrate or environment, or separating of desired product form interfering substances. Each module comprises a series of membranes separated by a solid support material which is channeled to provide a series of parallel capillaries for the flow of nutrients, environmental regulating fluids, or gas exchange. The structure and composition of the separating membranes limits the nature, rate, and size of the particular material exchanged across an individual membrane into or from an adjacent compartment. The capillaries are so dimensioned that the nutrients and gases are diffused over distances typical of human tissue.
    Type: Grant
    Filed: October 2, 1984
    Date of Patent: April 28, 1987
    Assignee: Cell Environmental Systems, Ltd.
    Inventors: Eric S. Berry, Bernard R. Danti
  • Patent number: 4656127
    Abstract: A method of detecting a mutation of a specific nucleotide base in a target nucleic acid chain comprises: (a) hybridizing a labelled probe to the target to form a hybrid in which one end of the probe is positioned adjacent the specific base; (b) adding a nucleotide derivative, e.g. a thionucleotide, under conditions to cause it to join to the end of the probe if it is complementary to the specific base; (c) digesting the hybrid using an exonuclease enzyme under conditions such that the nucleotide derivative protects the probe from digestion; and observing the presence or absence of label attached to the target. The method can be used to detect mutations even when these are not present at restriction enzyme cleavage sites, and does not require the preliminary steps of restriction digestion, gel electrophoresis and DNA (or RNA) blotting.
    Type: Grant
    Filed: April 23, 1984
    Date of Patent: April 7, 1987
    Assignee: Amersham International plc.
    Inventor: Christopher R. Mundy
  • Patent number: 4652517
    Abstract: Methods for the detection and identification of unknown pathogens or genetic entities from samples suspected of containing the same involve the use of novel wicking techniques whereby sample DNA/RNA becomes readily available on a porous, inert, positively charged support in single stranded form for hybridization with a hybridization probe having a nucleotide sequence substantially complementary to a nucleotide sequence of the sample DNA/RNA. Such methods permit the detection and identification of unknown pathogens and genetic entities in a shorter period of time than that required by conventional methods. Lysis solutions for rapidly lysing bacterial cells without the aid of enzymes are also disclosed.
    Type: Grant
    Filed: June 11, 1984
    Date of Patent: March 24, 1987
    Assignee: Diagnostic Research Limited Partnership
    Inventors: David R. Scholl, Joseph D. Jollick
  • Patent number: 4650770
    Abstract: Assays are provided employing particles and absorbent particles, wherein the absorbent particles substantially inhibit fluorescence when bound to the fluorescent particles through specific non-covalent binding.
    Type: Grant
    Filed: December 7, 1983
    Date of Patent: March 17, 1987
    Assignee: Syntex (U.S.A.) Inc.
    Inventors: Yen-Ping Liu, Edwin F. Ullman, Martin J. Becker
  • Patent number: 4618588
    Abstract: A reagent and merchandizing kit for use in the diagnosis of syphilis by hemagglutination of an anitgen obtained from a culture of pathogenic Treponema pallidum Nichols and which is sensitized on carrier particles in the presence of antibody wherein said antigen is substantially devoid of proteinic fractions of said culture having a specific gravity of less than 1.01, and methods of preparing the same.
    Type: Grant
    Filed: September 30, 1982
    Date of Patent: October 21, 1986
    Assignee: Fujizoki Pharmaceutical Co., Ltd.
    Inventors: Takashi Sato, Emiko Kubo
  • Patent number: 4608337
    Abstract: The present invention provides a stable, continuous human myeloma cell line which is capable of hybridization with antibody-producing cells of humans and other animals, the cell line being a mutant of GM 1500 human B cells and being deficient in hypoxanthine phosphoribosyltransferase. The invention also comprises processes for the production of hybrid cells employing the stable, HPRT-deficient human myeloma cell line, and processes for the production of antibodies employing such hybrid cells.
    Type: Grant
    Filed: May 17, 1983
    Date of Patent: August 26, 1986
    Assignee: The Wistar Institute
    Inventor: Carlo M. Croce
  • Patent number: 4605630
    Abstract: An agglutination assay reagent and method. The reagent is composed of liposomes predominantly in the 1 to 20 micron diameter size range. Each liposome has a surface array of laterally mobile ligand molecules, at a surface concentration adapted to produce reagent agglutination within about 5 minutes, when the reagent is incubated at room temperature with a multivalent ligand-binding analyte. A dye in the liposomes allows such agglutination to be visualized easily without magnification.
    Type: Grant
    Filed: July 27, 1983
    Date of Patent: August 12, 1986
    Assignee: Cooper Lipotech Inc.
    Inventors: Viola T. Kung, Francis J. Martin, Yolanda P. Vollmer
  • Patent number: 4598049
    Abstract: A microprocessor controlled, general purpose gene synthesizer for programmably synthesizing selected nucleotide sequences. Depending upon a programmed chemistry, metered volumes of selected bases and reagent/solvents are sequentially metered into the bottom of a reaction cell containing solid-supported nucleotides, where desired linkages are achieved in an agitated suspension. Self-refilling syringe metering and electrically initiated, pneumatically operated valving insures economical, non-contaminated synthesis. Preprogrammed, selectable chemistries and operator programmed sequences are operatively coupled under microprocessor control to ensure a general purpose synthesizer.
    Type: Grant
    Filed: August 31, 1983
    Date of Patent: July 1, 1986
    Assignee: Systec Inc.
    Inventors: Richard J. Zelinka, Keiichi Itakura, Carl W. Sims, Bruce E. Kaplan