Abstract: A multilayer analytical element for determining an assay of a component in whole blood sample is disclosed, which comprises at least two water-permeable layers, wherein one layer is a sample spreading layer and the other layer is a reagent layer, wherein one layer or more of said water-permeable layers contain a composition which interacts with said component in whole blood sample, wherein the composition forms a dye capable of being detected by a spectrophotometry at a wavelength of 600 n.m. or longer, when the composition interacts with said component in whole blood sample.