Patents Examined by Jon D. Epperson
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Patent number: 7469036Abstract: A method for determining atomic level structures of macromolecule-ligand complexes through high-resolution powder diffraction analysis and a method for providing suitable microcrystalline powder for diffraction analysis are provided. In one embodiment, powder diffraction data is collected from samples of polycrystalline macromolecule and macromolecule-ligand complex and the refined structure of the macromolecule is used as an approximate model for a combined Rietveld and stereochemical restraint refinement of the macromolecule-ligand complex. A difference Fourier map is calculated and the ligand position and points of interaction between the atoms of the macromolecule and the atoms of the ligand can be deduced and visualized. A suitable polycrystalline sample of macromolecule-ligand complex can be produced by physically agitating a mixture of lyophilized macromolecule, ligand and a solvent.Type: GrantFiled: May 31, 2002Date of Patent: December 23, 2008Assignee: Los Alamos National Security, LLCInventor: Robert B. Von Dreele
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Patent number: 7445784Abstract: This invention characterizes the specific peptide fragment derived from specially prepared zinc charged fetuin and a method of preparation thereof, wherein the fragment was found to contain an apoptosis-inducing activity. Specifically, the amino acid sequence of this peptide is His Thr Phe Ser Gly Val Ala Ser Val Glu (SEQ ID NO: 1). The peptide induced apoptosis in LNCaP (prostate cancer) and HT-29 (colon cancer) cells without affecting CCD 18 Co (normal colon) cells. The in vitro tissue culture study demonstrated that the peptide is more potent than the parent molecule (full-length zinc charged fetuin) in inducing apoptosis. The peptide has a LD50 of 0.3-0.4 ?M, while the LD50 for zinc-charged fetuin is 3-10 ?M.Type: GrantFiled: April 24, 2006Date of Patent: November 4, 2008Assignee: Ambryx Biotechnology, Inc.Inventor: David Tsai
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Patent number: 7442773Abstract: The present invention provides a universal peptide-binding scaffold. This scaffold is used to bind a target. The target can be a peptide or peptides of interest (for example, peptides associated with a disease state) or can represent the entire proteome. The target can be either protein fragments prepared by enzymatic digestion of the entire proteome or N- or C-terminal short sequences exposed by chemical denaturation of the entire proteome (unfolded proteins). The universal peptide-binding scaffold can be tailored to specifically bind a target using the methods described herein.Type: GrantFiled: January 21, 2005Date of Patent: October 28, 2008Assignee: The Board of Trustees of The University of IllinoisInventors: Huimin Zhao, Olga Esteban
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Patent number: 7442159Abstract: The present invention concerns a method for the selection of a virus comprising the steps of: (a) providing a virus encoding and displaying a fusion polypeptide, said fusion polypeptide comprising a heterologous polypeptide inserted into the sequence of a viral coat protein polypeptide, wherein said virus comprises a cleavable site located within a displayed polypeptide; (b) exposing the virus to a cleaving agent; (c) propagating the virus comprising intact fusion protein.Type: GrantFiled: November 10, 2000Date of Patent: October 28, 2008Assignee: Domantis LimitedInventors: Lutz Riechmann, Peter Kristensen, Jean-Luc Jestin, Gregory Paul Winter
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Patent number: 7435791Abstract: The present invention relates to a process for rapid solution synthesis of a peptide, the process comprising repetitive cycles of steps (a)-(d): (a) a coupling step, using an excess of an activated carboxylic component to acylate an amino component, (b) a quenching step in which a scavenger is used to remove residual activated carboxylic functions, wherein the scavenger may also be used for deprotection of the growing peptide, (c) one or more aqueous extractions and optionally, (d) a separate deprotection step, followed by one or more aqueous extractions, characterised in that the process comprises at least one step (b), referred to as step (b?), in which an amine or a thiol comprising a free anion or a latent anion is used as a scavenger of residual activated carboxylic functions. During the process of this invention the growing peptide need not be isolated until the final peptide sequence has been obtained.Type: GrantFiled: October 23, 2003Date of Patent: October 14, 2008Assignee: N.V. OrganonInventors: Ivo Franci Eggen, Paulus Bernardus Wilhelmus Ten Kortenaar, Cornelis Albert Gruson Haasnoot
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Patent number: 7429360Abstract: An automatic pipette in an analytic device uses disposable pipette tips, and in which proper coupling of the pipette tip to the pipette, accurate volume of the aspirated fluid, and distance of the pipette tip to a biochip are determined using a plurality of sensors that are coupled to the automatic pipette.Type: GrantFiled: May 28, 2003Date of Patent: September 30, 2008Assignee: Autogenomics, Inc.Inventors: Fareed Kureshy, Vijay K. Mahant, Shailendra Singh
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Patent number: 7427260Abstract: Disclosed are efficient methods for loading amino derivatives onto trityl chloride resins and for cleaving chemically modified amino derivatives from trityl chloride resins. Methods for making a library of discrete chemically modified amino derivatives also disclosed.Type: GrantFiled: March 29, 2002Date of Patent: September 23, 2008Assignee: Mayo Foundation for Medical Education and ResearchInventors: Yuan-Ping Pang, Jewn Giew Park
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Patent number: 7417129Abstract: A monosaccharide compound of formula I as shown in the specification. Processes for the preparation of the compound of formula I and methods of screening for antibacterial or antibiotic compounds involving the compound of formula I.Type: GrantFiled: April 17, 2003Date of Patent: August 26, 2008Assignee: Alchemia Pty LtdInventors: Michael Leo West, Wim Meutermans, George Adamson, Karl Schafer, Darren Schliebs
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Patent number: 7413536Abstract: A variety of methods for assaying libraries of test compounds as ligands and/or substrates of transport proteins, including both carrier-type and receptor-type transport proteins, are provided. Both in vitro and in vivo screening methods are disclosed. Also provided are methods for screening DNA libraries to identify members that encode transport proteins. Pharmaceutical compositions including compounds identified via the screening methods are also provided.Type: GrantFiled: September 14, 2000Date of Patent: August 19, 2008Assignee: XenoPort, Inc.Inventors: William J. Dower, Mark A. Gallop, Ronald W. Barrett, Kenneth C. Cundy, Tania Chernov-Rogan
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Patent number: 7407934Abstract: Methods for treating conditions associated with elevated, inappropriate or undesired post-prandial blood glucose levels are disclosed which comprise administration of an effective amount of an amylin agonist alone or in conjunction with other anti-gastric emptying agents. Methods for reducing gastric motility and delaying gastric emptying for therapeutic and diagnostic purposes are also described.Type: GrantFiled: August 18, 2003Date of Patent: August 5, 2008Assignee: Amylin Pharmaceuticals, Inc.Inventors: Orville G. Kolterman, Andrew A. Young, Timothy J. Rink, Kathleen Ann Keating Brown
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Patent number: 7399585Abstract: The system includes a microfabricated electrochemical biosensor for detecting the presence and/or quantity of the target analyte in the sample reagent. The biosensor includes a substrate and at least two electrically conductive electrodes. Each of the electrical conductive electrodes consists of a single layer of an electrically conductive material. The electrodes can be fabricated on the substrate by integrated circuit technology.Type: GrantFiled: May 3, 2001Date of Patent: July 15, 2008Assignee: The United States of America as represented by the Secretary of the NavyInventor: Vincent Jen-Jr. Gau
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Patent number: 7396940Abstract: Combinatorial libraries that contains various different 4, 5 fused 3-substituted-2-pyrrolocarboxylic acids for screening pharmacological activity and methods of synthesizing said libraries.Type: GrantFiled: October 1, 2004Date of Patent: July 8, 2008Assignee: Hoffmann-La Roche Inc.Inventors: Jianping Cai, Robert Alan Goodnow, Jr.
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Patent number: 7390787Abstract: Compositions and methods for treating infections, especially bacterial infections, are provided. Indolicidin peptide analogues containing at least two basic amino acids are prepared. The analogues are administered as modified peptides, preferably containing photo-oxidized solubilizer.Type: GrantFiled: January 24, 2003Date of Patent: June 24, 2008Assignee: Migenix Inc.Inventors: Janet R. Fraser, Michael H. P. West, Timothy J. Krieger, Robert Taylor, Douglas Erfle
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Patent number: 7390825Abstract: Substituted oxazolidinone of the formula: wherein R2 is alkyl selected from the group consisting of methyl, ethyl, and isopropyl moieties, are described. The compounds are antibacterial.Type: GrantFiled: October 17, 2002Date of Patent: June 24, 2008Assignee: Board of Trustees of Michigan State UniversityInventors: Rawle I. Hollingsworth, Guijun Wang, Raghavakaimal Padmakumar, Jianmin Mao, Huiping Zhang, Zongmin Dai, Kanakamma Puthuparampil
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Patent number: 7387865Abstract: A composition containing a photoacid generator monomer and surfactant, and a method for synthesizing a compound on a substrate using the composition are provided. The method includes bonding a layer of first molecules having an acid labile protecting group to a solid substrate; coating a layer of the photoacid generator monomer composition according to the present invention on the layer of first molecules; exposing the composition layer to light and then heat-treating to remove the acid labile protecting group from the first molecules corresponding to the exposed portion; washing and removing the composition layer from the exposed and unexposed portions; and bonding second molecules to the exposed first molecules.Type: GrantFiled: July 20, 2004Date of Patent: June 17, 2008Assignee: Samsung Electronics Co., Ltd.Inventors: Sung-ouk Jung, Seung-ju Seo, Jae-chan Park
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Patent number: 7385086Abstract: This invention provides compounds and methods for treating, with said compound, a mycobacterial infection by administering to an animal a pharmaceutical composition containing a compound having the formula R—SOn-Z-CO—Y, where R is an alkyl groups having 6-20 carbon atoms, unsaturated hydrocarbon groups having 6-20 carbon atoms, or alkyl groups having 6-20 carbon atoms interrupted by at least one aromatic ring; Z is —CH2—, —CH2CH2—, —NH—NH—, —O—, ——NH—, —O—NH—, —CH2—NH—, —CH2—O—, —NH—O—, —NH—CH2—, —O—CH2—, and —CH?CH—; Y is —NH2, —O—CH2—C6H5, —CO—CO—O—CH3, and —O—CH3; and n is 1 or 2. It has been discovered that these compounds treat microbially-based infections caused by corynebacteria, nocardiae, rhodococcus, and mycobacteria. These compounds may be used to treat mycobacterial cells, such as Mycobacteria tuberculosis, drug resistant M. tuberculosis, M. avium intracellulare, M. leprae, M. paratuberculosis, and pathogenic Mycobacteria sp.Type: GrantFiled: January 27, 2004Date of Patent: June 10, 2008Assignee: The Johns Hopkins University School of MedicineInventors: Craig A. Townsend, James D. Dick, Gary R. Pasternack, Francis P. Kuhajda, Nicole M. Parrish
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Patent number: 7384387Abstract: Apparatus and methods for high throughput mass spectrometry are provided. The methods involve sample preparation in an off-line parallel purification system. Such methods include but are not limited to the use of an appropriate buffer when generating samples or the use of a solid support for tagged components. The samples prepared in this way do not then need to be column separated. The apparatus provided includes a cell growth plate for growing cells and generating products and/or reactants, an off-line parallel purification system, a mass spectrometer, and an automatic sampler that transports samples and injects them into the mass spectrometer of the apparatus. The methods and apparatus described are used, for example, in screening enzyme reaction pathways.Type: GrantFiled: February 11, 2000Date of Patent: June 10, 2008Assignee: Maxygen, Inc.Inventors: Sun Ai Raillard, Yong Hong Chen, Claus Krebber, Jeremy Minshull, Willem P. C. Stemmer
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Patent number: 7371849Abstract: The present invention provides camel antibody libraries that maintain in vivo diversity of camelid antibody variable region genes. The in vivo diversity of antibody variable region genes can be accomplished by, for example, mixing genes derived from a plurality of animals or modifying gene amplification conditions. Conventional methods yield only VHHs with limited repertoire diversity. However, the present invention provides libraries comprising genes encoding functional VHHs with sufficient repertoire size. According to the present invention, libraries that enable to freely obtain VHHs against arbitrary antigens are provided. VHHs have excellent solubility and stability, and show a reactivity that usually cannot be expected from tetrameric IgGs.Type: GrantFiled: September 13, 2002Date of Patent: May 13, 2008Assignee: Institute For Antibodies Co., Ltd.Inventors: Toshio Honda, Yasushi Akahori, Yoshikazu Kurosawa
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Patent number: 7368528Abstract: The present invention provides antineoplastic peptides of formula I, R1R2N—CHX—CO-A-B-D-E-(G)s-K I wherein R1, R2, X, A, B, D, E, G, K, and s have the meanings stated in the description. The compounds have antineoplastic activity.Type: GrantFiled: July 11, 2005Date of Patent: May 6, 2008Assignee: Abbott GmbH & Co. KGInventors: Wilhelm Amberg, Teresa Barlozzari, Harald Bernard, Ernst Buschmann, Andreas Haupt, Hans-Guenther Hege, Bernd Janssen, Andreas Kling, Helmut Lietz, Kurt Ritter, Martina Ullrich, Jurgen Weyman, Thomas Zierke
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Patent number: 7361133Abstract: Viruses expressing ligands on their surfaces are used as a detection means for the related polypeptide which binds the ligand. Multiple copies of the ligand can be expressed on the viral surface. These viruses may be used to detect polypeptides, cells, receptors and channel proteins.Type: GrantFiled: November 30, 2000Date of Patent: April 22, 2008Assignee: The Johns Hopkins UniversityInventor: Min Li