Abstract: A fluorescence immunochromatographic detection card and a preparation method therefor and usage thereof is disclosed. The fluorescence immunochromatographic detection card comprises a treatment liquid A, a treatment liquid B, and a detection card. The treatment liquid A contains an antibody 15C4 that is coupled with a fluorescent microsphere. The treatment liquid B contains an antibody 13G12 that is coupled with biotin. The detection card comprises a detection line area and a quality control line area, and a streptavidin detection T line is fixed in the detection area, and an antibody quality control C line is immobilized in the quality control line area. The preparation method comprises: (1) formulating the treatment liquid A; (2) formulating the treatment liquid B; and (3) drawing the line on the detection card.

Abstract: A sample loading cartridge (1) for a microfluidic device comprises a cartridge body (10) with a sample reservoir (20) configured to house a volume of a liquid sample (3) and a sample port (30) in connection with the sample reservoir (20). The cartridge (1) also comprises an output channel (40) extending from the sample reservoir (20) and a feedback channel (50) connected to the sample reservoir (20) and to the sample port (30). The cartridge body (10) comprises a detection portion (60) aligned with the feedback channel (50) to enable detection of any sample (3) in the feedback channel (50). The flow resistance of the feedback channel (50) is lower than the flow resistance of the output channel (40) to cause liquid sample (3) received in the sample port (30) to enter the feedback channel (50) with substantially no liquid sample (3) entering the output channel (40).

Abstract: An automated analyzer is provided that includes a sample probe for dispensing a substance to be dispensed into a plurality of reaction vessels for causing a sample to be analyzed to react with a reagent, a measurement unit (e.g., light source, spectrophotometer, and control device) for measuring a reaction liquid produced from the sample and reagent in a reaction vessel, a cleaning tank for probe cleaning, and a control device for controlling the operation of the probe, measurement unit, and cleaning tank. The cleaning tank includes a cleaning pool for storing cleaning water for immersing and cleaning the probe and a drying tank for sucking up the cleaning water on the surface of the probe after the probe has been immersed in the cleaning water of the cleaning water pool and cleaned. As a result, it is possible to reduce the amount of cleaning water used to clean the probe.

Abstract: The present invention relates to a method of inhibiting, delaying, or reversing cellular senescence that includes having an isolated cell flow through a microchannel, and crashing the cell in flow into an impact surface installed on a flow path of the cell to apply a physical impact to the cell, resulting in inhibiting, delaying, or reversing senescence of the cell, while maintaining high biological activity, and in particular, it relates to a method that can further increase the value of a stem cell as a therapeutic cell for various degenerative diseases by maintaining undifferentiated state of the stem cell even during a long-term culture period to maintain multipotency, and to the cells obtained by the method.

Abstract: The automatic analyzer includes a housing unit capable of housing a plurality of reagent bottles each of which has an IC tag on one surface of short-side side surfaces thereof, an antenna used to communicate with the IC tag, a detector that detects the presence or absence of a reagent bottles, and a control unit. The housing unit includes a housing container cooled by a cooling machine, a heat insulating material, a reagent tray provided with a plurality of reagent bottle holding portions that hold the reagent bottles, a disk rotatably holding the reagent tray, and a driving unit driving the disk, and when it is unable to communicate with the IC tag of the reagent bottle installed in the reagent bottle holding portion where the reagent bottle is detected by the detector, the control unit determines that the reagent bottle is installed in a reverse direction.

Abstract: A microfluidic detection unit comprises at least one fluid injection section, a fluid storage section and a detection section. Each fluid injection section defines a fluid outlet; the fluid storage section is in gas communication with the atmosphere and defines a fluid inlet; the detection section defines a first end in communication with the fluid outlet and a second end in communication with the fluid inlet. A height difference is defined between the fluid outlet and the fluid inlet along the direction of gravity. When a first fluid is injected from the at least one fluid injection section, the first fluid is driven by gravity to pass through the detection section and accumulate to form a droplet at the fluid inlet, such that a state of fluid pressure equilibrium of the first fluid is established.

Abstract: The present disclosure provides an automatic and portable system, comprising a bench-top instrument and a disposable microfluidic device for multiplex detection and quantitation of biomolecules such as proteins and nucleic acids from biological samples. The disposable device pre-encapsulates all the reagents including capture probes, detection probes, and wash buffer, with a waste reservoir in it, allowing all the liquids to be circulated inside the device, without interaction with the external environment. The bench-top instrument comprises a pressure control module and a fluorescence detection module, enabling automatic device operation and signal detection without manual intervention.

Abstract: A method of testing a biological sample, the method comprising: providing a centrifuge sample holder having a camera; arranging a transparent container comprising a fluid test medium in a recess of said centrifuge sample holder, wherein the camera is arranged to image a portion of the container comprising said fluid test medium; arranging said biological sample above and not in contact with said fluid test medium in said container; and with the camera, imaging a mixing of said biological sample with said fluid test medium while centrifuging the sample holder.

Abstract: This invention relates to a sampling device. The device includes an elongate separating member having a sampling side and a non-sampling side. One or more through openings extend from the sampling side to the non-sampling side of the elongate member. The separating member is adapted for insertion into a reservoir of particulate material so as to define a sampling zone and a non-sampling zone within the reservoir. A shaft is positioned away from the sampling side and operably associated with the separating member, wherein the shaft is selectively rotatable about its longitudinal axis. One or more sample capturing scoops are attached to the shaft so as to be aligned with a respective opening. The or each scoop has a leading edge, a trailing edge and a cavity for receiving a sample of particulate material. The device is configured such that rotation of the shaft about its longitudinal axis causes a corresponding rotation of the or each scoop between a first position and a second position.

Abstract: The invention provides kits, devices, methods, and systems for forming droplets or particles and methods of their use. The devices may be used to form droplets of a size suitable for utilization as microscale chemical reactors, e.g., for genetic sequencing. In general, droplets are formed in a device by flowing a first liquid through a channel and into a droplet formation region including a second liquid, i.e., the continuous phase. The invention allows for more efficient recovery of droplets or processed droplets.

Abstract: A milking system includes a milking device, a milk line, and a sampling and analysis device for milk from the milk line, that includes a control unit, a carrier with reagent pads to detect a substance in the sample, a dosing device to dose a sample onto a reagent pad, an sensor device to detect radiation from that reagent pad, and to analyse the detected radiation to indicate a presence or concentration of said substance. The dosing device includes a nozzle with a supply line, a pump for pumping liquid to the nozzle, a flat wall part, and a mover to press the flat wall part and the nozzle against each other to close the nozzle. In this way, the nozzle can be filled completely to a known level and without bubbles. Thereafter, the sample pump can provide a sample droplet with known volume, which enables better control over the sample supply process.

Abstract: Spherical grains and sacrificial particles are mixed in a suspension. The sacrificial particles are larger than the spherical grains. The suspension is injected into a channel in a microfluidic chip, and the spherical grains form microporous structures in the channel. The microporous structures are sintered in the channel. A solvent is injected into the channel, and the solvent dissolves the sacrificial particles and forms macropores between at least some of the microporous structures, thereby forming a mixed-porosity microfluidic chip.

Abstract: A concentration device that concentrates a solution containing a biological substance includes: a first chamber into which the solution is to be introduced; a second chamber to be filled with a drawing agent; and a film that separates a space of the first chamber and a space of the second chamber. The film has a first region and a second region. The first region is semipermeable in that the first region is permeable to a solvent of the solution but impermeable to the biological substance and the drawing agent. The second region is impermeable to the solution, the biological substance, and the drawing agent. When the solution is introduced into the first chamber, the film is positioned to incline with respect to a surface of the solution. The second region is positioned below the first region with respect to the surface of the solution.

Abstract: A magnetic digital microfluidic apparatus for manipulating a liquid droplet containing magnetic particles using a magnetic force, the apparatus comprising: a hydrophobic surface on which the liquid droplet containing magnetic particles can be moved using the magnetic force; and at least one surface energy trap provided to retain at least a portion of the liquid droplet thereon, the at least one surface energy trap comprising a layer of polydopamine. A method of magnetic digital microfluidic manipulation, the method comprising the steps of: a) contacting a liquid droplet on a hydrophobic surface with a polydopamine surface energy trap, the liquid droplet containing magnetic particles; b) retaining at least a portion of the liquid droplet on the surface energy trap; and c) moving at least the magnetic particles with a magnetic force.

Abstract: A well plate assembly with an interior channel system in the well plate lid provides a more efficient and uniform distribution of fluid into a receptacle positioned below the well plate lid. The channel system in the lid allows air, or other fluid, to pass through with the use of a pump to each of a plurality of channels in the receptacle. Inlet and outlet valves in the lid prevent a gasket positioned between the lid and the receptacle from releasing contact with the receptacle due to high pressure experienced during the injection of fluid into the assembly. Specifically, pressure under a specified tolerance passes through the inlet valves, and if the pressure within the channel system exceeds the limit of the outlet valve, the outlet valve opens and allows air to escape the lid safely without disturbing the fluid flow into the channels below.

Abstract: Aspects of the disclosure relate to liquid chromatography (e.g., HPLC) methods which enable high resolution separations of polynucleotides of various lengths, sequences, and/or base compositions in a highly tunable manner. In some embodiments, the disclosure describes liquid chromatographic methods for separating a nucleic acid (e.g., a polyadenylated nucleic acid, such as an mRNA) from a complex mixture by using multiple ion pairing agents in the same mobile phase system. Accordingly, in some embodiments methods described by the disclosure are useful for assessing the quality of pharmaceutical preparations comprising nucleic acids.

Abstract: A microfluidic flow cell for carrying out an analysis, having a substrate of synthetic material, which has cavities for the formation of channel structures and chambers, wherein the cavities are closed on one side of the substrate by a film adhesively bonded or welded to the substrate, and having a housing component that is produced with a hard and a soft component, which, on the side of the substrate facing away from the film, is connected to the substrate, completing functional sections respectively fulfilling the function of the flow cell. The housing component is formed as a multifunctional part completing more than two functional sections.

Abstract: A microfluidic valve can include a substrate having a microfluidic channel formed in the substrate. A sealing layer can be over the microfluidic channel. A flexible blister layer can be over the sealing layer. The flexible blister layer can include a blister formed as a distended portion with a blister volume between the flexible blister layer and the sealing layer. The microfluidic valve can be actuatable by puncturing the sealing layer by pressing on the blister. Actuating the microfluidic valve can either allow fluid to flow through the microfluidic channel or block fluid from flowing through the microfluidic channel.

Abstract: An optofluidic diagnostic system and methods for rapid analyte detections. The system comprises an optofluidic sensor array, a test plate and an optical detection cartridge. The sensor array supports one or more distinct sensor units, each having a reactor section designed to temporarily enter a series of different kinds of wells in the test plate. One kind of well is a sample reservoir that holds reagent solution to be transferred into the reactor section. Another kind of well is a drainage chamber that removes reagent solution from the reactor section. A third kind of well is a colorant reservoir that holds a colorant reagent transferable into a reactor section. Finally, the sensor unit is transferred to the optical detection cartridge where it is placed into an isolation booth during the optical detection process so that its flat observation face is stationed in a viewing window opposite an optical detector lens.

Abstract: A microfluidic device for detection of an analyte in a fluid is described. The microfluidic device comprises a substrate having a first surface defining entrances to one or more chambers defined in the substrate, surfaces of the chambers defining a second surface of the substrate, the first surface being modified for selective targeting and capture of at least one analyte to operably effect a blocking of the entrance to at least one of the chambers, and wherein a response characteristic of the microfluidic device is operably varied by the blocking of the entrance to the at least one of the chambers, thereby providing an indication of the presence of the analyte within the fluid.