Abstract: Disclosed are a detection chip and a manufacturing method therefor, and a reaction system. The detection chip includes: a first substrate (11); a microcavity defining layer (12), which is located on the first substrate (11) and defines a plurality of micro-reaction chambers (120); and a shading structure layer (13), which is located on the first substrate (11) and provided among the plurality of micro-reaction chambers (120). In practical application, the number of target molecules in a reaction system solution in each micro-reaction chamber (120) can be determined by collecting a fluorescence image; and the detection chip is provided with the shading structure layer (13), and the shading structure layer (13) is located on the first substrate (11) and provided among the plurality of micro-reaction chambers (120).

Abstract: Smear preparation machine and control method therefor. The smear preparation machine includes a slide loading mechanism for loading slide, a sample applying mechanism for applying blood sample, a slide smearing mechanism for smearing blood sample to prepare smear slide, a staining mechanism and a slide transferring mechanism. The staining mechanism includes a reagent vessel for storing staining reagent and a first driving system for driving the reagent vessel to reciprocally translate in first direction. The slide transferring mechanism includes a manipulator and a second driving system for driving the manipulator to reciprocally translate in second direction. After reagent vessel and manipulator respectively translate to corresponding cooperative positions, the second driving system drives manipulator to move towards reagent vessel to place or remove smear slide in reagent vessel.

Abstract: Methods and systems for generating three-dimensional (3D) engineered tissues (ETs), and for electrical stimulation of same, are provided. Provided is an ET assembly comprising an ET lid with first post and second post assemblies coupled thereto. Provided is a casting assembly comprising the ET assembly and a casting plate. Provided are stimulation methods and systems for stimulating tissue constructs.

Abstract: A portable incubation system comprising a container and a temperature management system disposed within the container. The container comprises a housing forming an outer wall of the container, an inner chamber assembly disposed within the housing, and a lid coupled to the housing and arranged to seal the inner chamber assembly when closed. The temperature management system comprises a heat sink in thermal communication with an ambient environment of the portable incubation system, a thermoelectric cooler coupled to the inner chamber assembly, and a water-based heat pipe comprises a first end coupled to the heat sink and a second end coupled to the thermoelectric cooler. The system comprises a control system configured to direct the temperature management system to regulate a temperature of the inner chamber assembly and power the portable incubation system.

Abstract: The present invention relates to methods and transformed host cells for the production of eriodictyol from naringenin via bioconversion.

Abstract: Disclosed are a biomimic system simulating lung tissue, a method for manufacturing same, and a microfluidic control method using same, wherein the biomimic system comprises lung epithelial cells and lung fibroblasts, which are isolated from human lungs, and commercially available vascular endothelial cells, and wherein a microfluid flows through the biomimic system. Each chamber inside the corresponding system can allow a fluid, which contains gas and a medium, to flow therethrough and simulate respiration-like movement, wherein all of the three types of cells can survive inside the system even when one week or more have elapsed after through-flow of the fluid. In addition, the pH and pO2 in the chamber can be monitored by using a pH sensor and a gas partial pressure sensor inside the system, and thus the three types of cells inside the system can be exposed to external environments, drugs, and the like under the same conditions as in the lungs in vivo.

Abstract: Disclosed herein are devices, systems and methods for the hypothermic storage of biological samples, such as tissues, organs, or body fluids between arrival at a transplantation site and transplantation. In some examples, various cooling media may be positioned in and around a container used to for the hypothermic storage.

Abstract: A reaction processor includes: a reaction processing vessel; a first optical head including a first objective lens OB1 for irradiating a sample with first excitation light and collecting first fluorescence generated from the sample; a second optical head including a second objective lens for irradiating a sample with second excitation light and collecting second fluorescence generated from the sample; and a holding member holding the first optical head and the second optical head. The wavelength range of the first fluorescence and the wavelength range of the second excitation light at least partially overlap with each other. A distance between the optical axis of the first objective lens and the optical axis of the second objective lens satisfies 2·P0+2·P1+4·P2+4·P3<P, P0=L·NA/?(1?NA2), P1=t1·NA/?(n12?NA2), P2=t2·NA/?(1?NA2), and P3=t3·NA/?(na32?NA2).

Abstract: A method for the determination of antibiotic susceptibility through stimulated Raman scattering microscopy is disclosed. The method utilizes a imaging apparatus adapted to collect a laser signal through a sample having a bacteria for imaging the metabolism of the sample. The sample can be manipulated with an antibiotic for imaging to determine the susceptibility of a bacteria to the provided antibiotic.

Abstract: One or more embodiments of the present disclosure relate to a culture device, which comprises: an accommodation base, an accommodation chamber disposed on the accommodation base. The accommodation chamber includes a culture chamber for accommodating one or more cultures. The culture chamber includes a chamber bottom wall and a chamber side wall surrounding the chamber bottom wall. A culture solution channel is formed between an outer side of the chamber side wall and an inner wall of the accommodation chamber. At least part of the chamber side wall includes a membrane material, and a plane where the membrane material is located intersect with the chamber bottom wall.

Abstract: One aspect of this disclosure is a method for maintaining a plankton population in a culture medium by removing particles from the culture medium. The method may comprise rotating a filter body to lift the particles from the culture medium with a filter of the filter body, positioning the filter relative to a conduit so that a first portion of the lifted particles fall into the conduit, directing a removal fluid toward the filter body to move a second portion of the lifted particles off the filter and into the conduit with impact forces applied by the removal fluid, and/or outputting an effluent flow from the conduit. The effluent flow may comprise the first and second portions of the lifted particles and a portion of the removal fluid. Aspects of related apparatus, methods, and systems also are disclosed.

Abstract: There is described a fermentation tank for the multiplication of microorganisms, particularly a vertical fermentation tank comprising aeration and agitation elements with low cost and high efficiency. Particularly, a fermentation tank for the multiplication of microorganisms (100) comprising an external structure (10) of longitudinal axis (L) and an inner container (20) which receives a mixture to be fermented, the fermentation tank (100) comprising an aeration and agitation element (30) arranged in the inner container (20) collinear with the longitudinal axis (L), the aeration and agitation element (30) comprising internally a plurality of sets of fins (40), each set of fins (40) being arranged axially parallel to each other along the longitudinal axis (L) to provide aeration and agitation of the mixture to be fermented.

Abstract: A cell culture insert for use in culturing cells to promote the formation of spheroids and methods of using these spheroid-promoting cell culture inserts. The cell culture insert includes a porous membrane and one or more sidewalls that are non-adherent to cells and cause the cells in the insert to associate with each other and form spheroids.

Abstract: A multilayer culture vessel includes: an internal space that is divided by a boundary portion into a culture space on one side and a buffer space on the other side in a direction parallel to a bottom plate; at least one intermediate plate extending along the direction parallel to the bottom plate at least in the culture space and configured to divide the culture space into culture layers; wall portions at the boundary portion, the wall portions including one extending from the bottom plate in a direction toward a top plate and one extending from each of the at least one intermediate plate in a direction toward the top plate; communication portions formed at the boundary portion to bring the culture layers into communication with the buffer space; and a liquid supply/drainage port formed in the housing at a location facing the buffer space.

Abstract: Electronic sensors configured to detect single molecules and DNA methods of using and manufacturing same are disclosed. A sensor may include source and drain electrodes spaced apart by a sensor gap; a gate electrode, wherein the source, drain and gate electrodes cooperate to form an electrode circuit; and a bridge molecule bridging across the sensor gap, connecting source and drain electrodes; and a probe coupled to the bridge molecule, wherein interaction of the probe with a nucleic acid is detectable by monitoring a parameter of the electrode circuit. In various examples, the nucleic acid comprises DNA or RNA.

Abstract: The present disclosure relates to Oxygen Reduction Disposable kits (ORDKit), devices and methods for the improved preservation of whole blood and blood components. The improved devices and methods for the collection of blood and blood components provide for whole blood and blood components having reduced levels of oxygen. The devices and methods provide for the rapid preparation of deoxygenated blood and blood components for storage that improves the overall quality of the transfused blood and improves health outcomes in patients.

Abstract: A pressure generating device includes a tank, a deformable membrane, a driving device, and an actuating arm. The tank defines a chamber therein, and includes an opening and a communication port each of which is in fluid communication with the chamber. The deformable membrane is disposed to seal the opening, and is deformable between a flat state and a deformed state. The actuating arm is coupled to be driven by the driving device to move between a first position, where the deformable membrane is in the flat state, and a second position, where the deformable membrane is forced to be in the deformed state, such that a predetermined negative pressure is generated through the communication port when the actuating arm is driven from one of the first and second positions to the other one of the first and second positions.

Abstract: Provided in the present invention is an incubator capable of producing pressure fluctuations. The incubator comprises an incubator body and a variable pressure apparatus, the incubator body having a variable pressure interface, the variable pressure apparatus being connected to the incubator body by means of the variable pressure interface, and the variable pressure apparatus being used for inputting fluctuating or constant air pressure into the incubator body.

Abstract: According to one embodiment, a nucleic acid construct is used for detecting an estrogen-sensitive cell. The nucleic acid construct includes an enhancer sequence, a promoter sequence and a reporter gene. The enhancer sequence includes at least an ERE sequence and at least a XRE sequence. The promoter sequence is ligated to downstream of the enhancer sequence. The reporter gene is ligated to downstream of the promoter sequence.

Abstract: An apparatus for culturing suspended photosynthetic organisms, specifically a flowing thin film photobioreactor, wherein the flowing thin film photobioreactor comprises a light source and a trickle-film insert comprising screen material.