Abstract: Disclosed herein are devices, systems and methods for the hypothermic storage of biological samples, such as tissues, organs, or body fluids between arrival at a transplantation site and transplantation. In some examples, various cooling media may be positioned in and around a container used to for the hypothermic storage.

Abstract: A reaction processor includes: a reaction processing vessel; a first optical head including a first objective lens OB1 for irradiating a sample with first excitation light and collecting first fluorescence generated from the sample; a second optical head including a second objective lens for irradiating a sample with second excitation light and collecting second fluorescence generated from the sample; and a holding member holding the first optical head and the second optical head. The wavelength range of the first fluorescence and the wavelength range of the second excitation light at least partially overlap with each other. A distance between the optical axis of the first objective lens and the optical axis of the second objective lens satisfies 2·P0+2·P1+4·P2+4·P3<P, P0=L·NA/?(1?NA2), P1=t1·NA/?(n12?NA2), P2=t2·NA/?(1?NA2), and P3=t3·NA/?(na32?NA2).

Abstract: A method for the determination of antibiotic susceptibility through stimulated Raman scattering microscopy is disclosed. The method utilizes a imaging apparatus adapted to collect a laser signal through a sample having a bacteria for imaging the metabolism of the sample. The sample can be manipulated with an antibiotic for imaging to determine the susceptibility of a bacteria to the provided antibiotic.

Abstract: One or more embodiments of the present disclosure relate to a culture device, which comprises: an accommodation base, an accommodation chamber disposed on the accommodation base. The accommodation chamber includes a culture chamber for accommodating one or more cultures. The culture chamber includes a chamber bottom wall and a chamber side wall surrounding the chamber bottom wall. A culture solution channel is formed between an outer side of the chamber side wall and an inner wall of the accommodation chamber. At least part of the chamber side wall includes a membrane material, and a plane where the membrane material is located intersect with the chamber bottom wall.

Abstract: One aspect of this disclosure is a method for maintaining a plankton population in a culture medium by removing particles from the culture medium. The method may comprise rotating a filter body to lift the particles from the culture medium with a filter of the filter body, positioning the filter relative to a conduit so that a first portion of the lifted particles fall into the conduit, directing a removal fluid toward the filter body to move a second portion of the lifted particles off the filter and into the conduit with impact forces applied by the removal fluid, and/or outputting an effluent flow from the conduit. The effluent flow may comprise the first and second portions of the lifted particles and a portion of the removal fluid. Aspects of related apparatus, methods, and systems also are disclosed.

Abstract: There is described a fermentation tank for the multiplication of microorganisms, particularly a vertical fermentation tank comprising aeration and agitation elements with low cost and high efficiency. Particularly, a fermentation tank for the multiplication of microorganisms (100) comprising an external structure (10) of longitudinal axis (L) and an inner container (20) which receives a mixture to be fermented, the fermentation tank (100) comprising an aeration and agitation element (30) arranged in the inner container (20) collinear with the longitudinal axis (L), the aeration and agitation element (30) comprising internally a plurality of sets of fins (40), each set of fins (40) being arranged axially parallel to each other along the longitudinal axis (L) to provide aeration and agitation of the mixture to be fermented.

Abstract: A cell culture insert for use in culturing cells to promote the formation of spheroids and methods of using these spheroid-promoting cell culture inserts. The cell culture insert includes a porous membrane and one or more sidewalls that are non-adherent to cells and cause the cells in the insert to associate with each other and form spheroids.

Abstract: A multilayer culture vessel includes: an internal space that is divided by a boundary portion into a culture space on one side and a buffer space on the other side in a direction parallel to a bottom plate; at least one intermediate plate extending along the direction parallel to the bottom plate at least in the culture space and configured to divide the culture space into culture layers; wall portions at the boundary portion, the wall portions including one extending from the bottom plate in a direction toward a top plate and one extending from each of the at least one intermediate plate in a direction toward the top plate; communication portions formed at the boundary portion to bring the culture layers into communication with the buffer space; and a liquid supply/drainage port formed in the housing at a location facing the buffer space.

Abstract: The present disclosure relates to Oxygen Reduction Disposable kits (ORDKit), devices and methods for the improved preservation of whole blood and blood components. The improved devices and methods for the collection of blood and blood components provide for whole blood and blood components having reduced levels of oxygen. The devices and methods provide for the rapid preparation of deoxygenated blood and blood components for storage that improves the overall quality of the transfused blood and improves health outcomes in patients.

Abstract: Electronic sensors configured to detect single molecules and DNA methods of using and manufacturing same are disclosed. A sensor may include source and drain electrodes spaced apart by a sensor gap; a gate electrode, wherein the source, drain and gate electrodes cooperate to form an electrode circuit; and a bridge molecule bridging across the sensor gap, connecting source and drain electrodes; and a probe coupled to the bridge molecule, wherein interaction of the probe with a nucleic acid is detectable by monitoring a parameter of the electrode circuit. In various examples, the nucleic acid comprises DNA or RNA.

Abstract: A pressure generating device includes a tank, a deformable membrane, a driving device, and an actuating arm. The tank defines a chamber therein, and includes an opening and a communication port each of which is in fluid communication with the chamber. The deformable membrane is disposed to seal the opening, and is deformable between a flat state and a deformed state. The actuating arm is coupled to be driven by the driving device to move between a first position, where the deformable membrane is in the flat state, and a second position, where the deformable membrane is forced to be in the deformed state, such that a predetermined negative pressure is generated through the communication port when the actuating arm is driven from one of the first and second positions to the other one of the first and second positions.

Abstract: Provided in the present invention is an incubator capable of producing pressure fluctuations. The incubator comprises an incubator body and a variable pressure apparatus, the incubator body having a variable pressure interface, the variable pressure apparatus being connected to the incubator body by means of the variable pressure interface, and the variable pressure apparatus being used for inputting fluctuating or constant air pressure into the incubator body.

Abstract: According to one embodiment, a nucleic acid construct is used for detecting an estrogen-sensitive cell. The nucleic acid construct includes an enhancer sequence, a promoter sequence and a reporter gene. The enhancer sequence includes at least an ERE sequence and at least a XRE sequence. The promoter sequence is ligated to downstream of the enhancer sequence. The reporter gene is ligated to downstream of the promoter sequence.

Abstract: An apparatus for culturing suspended photosynthetic organisms, specifically a flowing thin film photobioreactor, wherein the flowing thin film photobioreactor comprises a light source and a trickle-film insert comprising screen material.

Abstract: The technology disclosed herein relates to, at least in part, a removable imaging cassette. An illumination plate is configured to transmit light. A cassette body defines an outer surface and a cassette cavity within the outer surface, where the outer surface surrounds the illumination plate. The cassette cavity abuts the illumination plate. A light source is disposed in the cassette cavity. Mating electrical contacts extend through the outer surface of the cassette body, where the mating electrical contacts are operably coupled to the light source.

Abstract: A sparger assembly for a bioprocessing system includes a base and a plurality of spargers connected to the base, each sparger including a plurality of pores, the plurality of spargers each have a generally cylindrical shape. Each of the plurality of spargers includes a sidewall and a top, which define the cylindrical shape, the sidewall and the top each include a plurality of pores. The pores of the sidewall can be arranged around a circumference of the sidewall at an array of heights. Ridges may also be located on the sidewall above a respective array of pores.

Abstract: A carbon dioxide electrolytic device in an embodiment includes: an electrochemical reaction cell including: a first accommodation part that accommodates gas or a first electrolytic solution containing CO2; a second accommodation part that accommodates a second electrolytic solution containing H2O; a diaphragm provided between the first and second accommodation parts; a cathode that is in contact with the gas or the first electrolytic solution; and an anode that is in contact with the second electrolytic solution; a first supply part that supplies the gas or the first electrolytic solution to the first accommodation part; a second supply part that supplies the second electrolytic solution to the second accommodation part; and a carbon dioxide separation part that is connected to a discharge portion of a discharge containing O2 and CO2 from the second accommodation part and includes a cryogenic separation device to separate CO2 from a gas component in the discharge.

Abstract: The present disclosure relates to methods, apparatuses, and systems to measure one or more electrical signals from a cultured cell layer. In some embodiments, such electrical signals can be indicative of transport or lack of transport of an agent through the cell layer.

Abstract: The present invention provides a method for simple and effective monitoring of a dynamic change in a cell or a derivative thereof. More specifically, the present invention provides a method for monitoring a dynamic change in a cell, which includes preparing a plurality of compartments containing at least one cell or a derivative thereof and at least one bead, and repeatedly obtaining over time both state measurement information of the cell or the derivative thereof in each compartment, and imaging information of the bead to monitor a dynamic change in each cell or derivative thereof in the plurality of compartments, wherein the imaging information of the bead in each compartment can be clearly distinguished from each other and becomes an index of identifying the cell or the derivative thereof contained in each compartment.

Abstract: A method of pumping fluid out of a bioprocess vessel includes providing a bioprocess vessel defining an interior volume and having a bottom surface containing an aperture or port therein for the passage of fluid and a pump head integrally formed with or bonded to the bottom surface of the bioprocess vessel or secured to the port, wherein the pump head further comprises an inlet and an outlet with the inlet in fluid communication with the interior volume, the pump head further including a plurality of diaphragms, each diaphragm associated with a check-valve, wherein sequential actuation of the plurality of diaphragms pumps fluid from the interior volume of the bioprocess vessel into the inlet and out of the outlet. The plurality of diaphragms are actuated with a motor secured to the pump head. In other embodiments, the pump head is secured to the bioprocess vessel with fasteners.