Abstract: Provided are a photoactive fluorophore, a photoactive ligand, and a photoactive complex. The photoactive fluorophore includes a photoactivatable derivative of an azetidine-containing Janelia-Fluor dye. The photoactive ligand includes a photoactive fluorophore and a protein tag. The photoactive complex includes a photoactive ligand conjugated to a protein. Also provided are methods of in vivo labeling with and photoactivation of the photoactive fluorophore, ligand, and complex.
Abstract: System and method for providing type-II (and higher order) phase-locked loops (PLLs) with a fast signal acquisition mode. A preferred embodiment comprises a loop filter with a proportional loop gain path (proportional loop gain circuit 1115) and an integral loop gain block (integral loop gain block 1120). The proportional loop gain path is used during signal acquisition to provide large loop bandwidth, hence fast signal acquisition of a desired signal. Then, during the PLL's signal tracking phase, the integral loop gain block is enabled and its output is combined with output from the proportional loop gain path to provide higher order filtering of the desired signal. An offset that may be present due to the use of the proportional loop gain path can be measured and subtracted to help improve signal tracking settling times.
Type:
Grant
Filed:
June 19, 2003
Date of Patent:
December 5, 2006
Assignee:
Texas Instruments Incorporated
Inventors:
Robert B. Staszewski, Dirk Leipold, Khurram Muhammad