Abstract: A method and apparatus for fabricating a microporous-membrane for gel plate separating and transferring macromolecules by electrophoresis, the apparatus comprising a peripheral frame (10) provided with a removable bottom plate and including means (54, 58, 60, 64) enabling the membrane to be tensioned after it has been placed on the liquid gel as cast onto the bottom plate.
Abstract: A disclosure is made of automatic apparatus for processing transfer membranes to which a blot pattern of molecular fragments has been bound so that the pattern may be optically visualized. The apparatus is characterized by at least one open processing cell and two systems for pumping; one selectively controlled to direct fluid to and from functional units and one which is closely coupled to the cell and recirculates the fluid in the cell to assure adequate and thorough treatment therein by uniform fluid flow across both sides of the membrane.
Type:
Grant
Filed:
December 29, 1992
Date of Patent:
September 27, 1994
Assignee:
E. I. Du Pont de Nemours and Company
Inventors:
Donald H. Brunk, David J. Regester, Charles W. Robertson, Anders J. Wellings