Abstract: A process of purifying or enriching coagulation FVIII employing chromatography comprising the steps of providing a fraction containing FVIII in an aqueous solution having a high ionic strength; contacting the fraction containing FVIII with a multimodal resin; optionally washing the multimodal resin having FVIII adsorbed with an aqueous washing buffer; eluting FVIII containing fractions by an aqueous elution buffer comprising at least one amino acid which is positively charged at pH 6 to 8; and optionally collecting FVIII containing fractions in purified or enriched form.
Type:
Grant
Filed:
June 24, 2009
Date of Patent:
December 11, 2012
Assignee:
Octapharma AG
Inventors:
Carin Borgvall, Ulrika Ericsson, Gustav Gilljam, Mats Jernberg, Stefan Winge