Patents Examined by Karen I. Krupen
  • Patent number: 5008199
    Abstract: Monoclonal antibody to Treponema denticola ATCC 33520 and a continuous cell line for its production is disclosed. These antibodies are particularly useful in immunoassays for detecting the presence of these microorganisms in the oral cavity.
    Type: Grant
    Filed: November 19, 1987
    Date of Patent: April 16, 1991
    Assignee: Warner-Lambert Company
    Inventors: Dou-Mei Wang, Linda D. Sturdivant, Thomas A. Biemer, Ronald Mink
  • Patent number: 4978609
    Abstract: The present invention relates to monoclonal antibodies having high affinity to human pancreatic PLA.sub.2, the production thereof, hybridomas producing them and an immunoassay for human pancreatic PLA.sub.2 using them.
    Type: Grant
    Filed: March 25, 1988
    Date of Patent: December 18, 1990
    Assignee: Shionogi & Co., Ltd.
    Inventors: Atsushi Misaki, Masao Kono, Michio Ogawa, Mitsuhiro Okamoto
  • Patent number: 4978608
    Abstract: Picogram amounts of DNA can be detected in a sample by the use of high affinity single-stranded DNA binding proteins. The assay is applicable not only to pure DNA samples but also to samples containing significant amounts of protein.
    Type: Grant
    Filed: September 4, 1987
    Date of Patent: December 18, 1990
    Assignee: Molecular Devices Corporation
    Inventors: Viola T. Kung, Peter A. Nagainis
  • Patent number: 4977086
    Abstract: Murine monoclonal antibodies specific to unique antigenic determinants on mammalian terminal deoxynucleotidyl transferases (TdT). The monoclonal antibodies specifically bind to TdT in a wide variety of mammalian cells including human, mouse, rat, rabbit and bovine origin. The monoclonal antibodies are secreted by hybridoma cell lines derived from fusion of murine plasmacytoma cells with splenocytes from mice immunized with TdT from bovine thymus cells. The monoclonal antibodies can detect small numbers of TdT-positive cells from monitoring of TdT-positive leukemias and lymphomas in multple species, including human.
    Type: Grant
    Filed: June 12, 1989
    Date of Patent: December 11, 1990
    Assignee: The Board of Regents of the University of Texas System
    Inventor: R. Graham Smith
  • Patent number: 4977081
    Abstract: Rabbit-mouse hybridomas are cultured immediately after fusion in a suspension medium supplemented with normal rabbit serum. This results in stable hybridomas and provides a source of intact rabbit monoclonal antibody immunoglobulin of defined specificity. A hybridoma which secretes intact rabbit antibody specific for Group A Streptococcus is exemplified. Diagnostic compositions and test kits containing rabbit monoclonal antibodies produced from such hybridomas are described.This application is a continuation of application Ser. No. 07/189,754, filed May 3, 1988, which in turn is a continuation-in-part of application Ser. No.: 046,439, filed May 4, 1987, now abandoned.
    Type: Grant
    Filed: April 4, 1990
    Date of Patent: December 11, 1990
    Assignee: Adi Diagnostics, Inc.
    Inventors: Torquil J. G. Raybould, Miyoko Takahashi
  • Patent number: 4975380
    Abstract: Thermochemically induced luminescence is generated in a fluorescent labeled organic compound containing a covalently bonded fluorescent label which is a polycyclic aromatic radical having at least three linearly fused benzene rings and capable of being excited to a fluorescent electronic excited state by energy transfer from an energy donor molecule or radical having an electronic excited state, by a process comprising generating an energy donor radical or molecule by a thermochemical reaction in the presence of the fluorescent label. The energy donor radical may be generated by a chemical reaction in the presence of the fluorescent labeled organic compound or the fluorescent labeled compound itself may be additionally labeled with a radical capable of being excited to an electronic excited state by a thermochemical reaction, i.e., by a process initiated by adsorption of thermal energy.
    Type: Grant
    Filed: November 30, 1989
    Date of Patent: December 4, 1990
    Assignee: Rijksuniversiteit Te Groningen
    Inventors: Jan C. Hummelen, Hans Wynberg
  • Patent number: 4971902
    Abstract: Oligonucleotide probes for diagnosing predisposition to rheumatoid arthritis, capable of specifically hybridizing with5'-TACGGGGTTGR.sub.1 GAGAGCTT-3'or3'-AR.sub.2 GCCCCAACR.sub.3 CR.sub.2 CR.sub.2 CGAA-5'wherein A is adenine, C is cytosine, G is guanine, T is thymine, R.sub.1 is GT or TG, R.sub.2 is T or uracil, and R.sub.3 is CA or AC. Or, capable of specifically hybridizing with5'-GGAGCAGAR.sub.2 GCGGGCCGCGG-3'or3'-CCR.sub.2 CGR.sub.2 CR.sub.2 R.sub.3 CGCCCGGCGCC-5'wherein R.sub.1 is A or G, R.sub.2 is T or uracil, and R.sub.3 is R.sub.2 or C.
    Type: Grant
    Filed: October 30, 1987
    Date of Patent: November 20, 1990
    Assignee: Virginia Mason Research Center
    Inventor: Gerald T. Nepom
  • Patent number: 4965198
    Abstract: A monoclonal antibody specific to a sugar chain containing N-glycolylneuraminic acid and has an ability to bind at least to N-glycolyl GM.sub.2 ganglioside, and producing methods of a hybridomas which produce the monoclonal antibodies are disclosed. The antibodies are extremely useful for clarification of carcinogenesis mechanism, cancer diagnosis and therapy. The hybridomas are manufactured by using an animal with autoimmune disease.
    Type: Grant
    Filed: April 26, 1988
    Date of Patent: October 23, 1990
    Assignee: Konica Corporation
    Inventors: Masahiko Yamasaki, Yoshitaka Nagai
  • Patent number: 4963658
    Abstract: Picogram amounts of DNA can be detected in a sample by the use of high-affinity, single-stranded DNA binding proteins. The assay is applicable not only to pure DNA samples but also to samples containing significant amounts of protein.
    Type: Grant
    Filed: December 15, 1988
    Date of Patent: October 16, 1990
    Assignee: Molecular Devices Corporation
    Inventors: Viola T. Kung, Peter A. Nagainis, Edward L. Sheldon, III
  • Patent number: 4959320
    Abstract: A monoclonal antibody is described, having a specific reactivity with a cancer-associated antigen selected from pancreas cancer-associated antigen, intestinum crassum cancer-associated antigen and hepatoma-associated antigen and has the following characteristics:(1) it exists in a blood serum of pancreas cancer, intestinum crassum cancer and hepatoma patients;(2) it comprises glycoproteins;(3) it has a molecular weight of about 700,000 to 1,500,000, as measured by gel-filtration and determined by comparison with that of a known protein;(4) it has a maximum absorption of 2800 nm when dissolved in 0.1 M acetic acid buffer solution (pH 4.5); and(5) it is released at least in a cultured supernatant medium of establihed cell line of COLO-201, TE-1, TE-2, TE-3, NRC-12, MKN-45 and KATO III. The monoclonal antibody is utilized as a reagent for detecting pancreas cancer, intestinum crassum cancer and hepatoma. A process for preparing the monoclonal antibody and a process for preparing the reagent are also disclosed.
    Type: Grant
    Filed: August 8, 1985
    Date of Patent: September 25, 1990
    Assignee: Green Cross Corporation
    Inventors: Yahiro Uemura, Kazumi Fukuyama, Takashi Kobayashi, Yoshiaki Kanou, Ryutaro Yamana, Eiji Kashiwagi, Tomokuni Taniguchi, Kazuaki Nakura, Masahiro Watanabe, Masayuki Nishida, Tadakazu Suyama
  • Patent number: 4957859
    Abstract: Preparation of novel polypeptide sequences spanning amino acid position 61 of the ras protein, said sequences being characterized as containing a leucine in that position instead of the glutamine found in normal ras protein, the utilization of such polypeptides to prepare immunogen compositions utilizing such polypeptide covalently linked to immunogenic carrier materials, the production of antibodies elicited by such polypeptides, the screening of such antibodies to provide monoclonals specific to the P21Tleu61 transforming protein and to immunoassay and use in immunochemical methods employing such antibodies to determine the presence of the p21TLeu61 transforming protein.
    Type: Grant
    Filed: February 16, 1988
    Date of Patent: September 18, 1990
    Assignee: Hoffmann-La Roche Inc.
    Inventors: Diane Bizub, Ellyn Fischberg-Bender, Anna M. Skalka
  • Patent number: 4952507
    Abstract: Antibodies specific for pancreatic alpha-amylase and methods for their production are described. These antibodies may be used for the detection and quantification of pancreatic alpha-amylase in biological fluids, tissue extracts, or extracts of stains of biological fluids.
    Type: Grant
    Filed: March 21, 1989
    Date of Patent: August 28, 1990
    Assignee: Humagen Incorporated
    Inventor: Michael P. Woodward
  • Patent number: 4952517
    Abstract: An assay procedure that is particularly valuable for detecting and/or determining the presence of threshold levels of analyte ligands in biological fluids. In one particularized and specialized aspect the disclosure is directed to procedures for detecting and/or determining threshold levels of hormone metabolites such as pregnanediol-3-glucuronide (P.sub.3 G) and estrone-3-glucuronide (E.sub.1 3G) in human urine. The assay consists of contacting a sample containing the analyte with a known amount of an antibody thereto and with a calibrated amount of the analyte itself that is conjugated to said solid support. When the level of the analyte in the sample exceeds a threshold level, such as 5 ug/ml for P.sub.3 G, the antibody will be insufficient to block all of the corresponding analyte on the solid support.
    Type: Grant
    Filed: February 8, 1988
    Date of Patent: August 28, 1990
    Assignee: Hygeia Sciences, Inc.
    Inventor: Izak Bahar
  • Patent number: 4950595
    Abstract: Mouse-human hybridoma, which produces monoclonal antivirus-human antibody reactive to herpes simplex virus, varicella zoster virus, and cytomegalo virus, obtained by allowing a human antibody cell, which is sensitized to virus or protein or glycoprotein arising from virus, to fuse with a mouse myeloma cell in the presence of mitogen in vitro.
    Type: Grant
    Filed: May 28, 1986
    Date of Patent: August 21, 1990
    Assignee: Teijin Limited
    Inventors: Yasuhiko Masuho, Yoh-ichi Matsumoto, Toru Sugano, Katsuhiko Tomibe
  • Patent number: 4946788
    Abstract: The invention relates to novel purified human immunoglobulin E binding factors (IgE-BFs), its individual optionally glycosylated proteins, and fragments thereof, processes for the purification of IgE-BFs, novel monoclonal antibodies to lymphocyte cellular receptors for IgE (Fc.sub..epsilon. R) crossreacting with IgE-Fs, derivatives thereof, processes for the preparation of these antibodies and their derivatives, hybridoma cell lines that produce these antibodies, processes for the preparation of said hybridoma cell lines, the use of the monoclonal antibodies and their derivatives for the qualitative and quantitative determination of IgE-BFs, test kits containing the monoclonal antibodies and/or their derivatives, the use of the monoclonal antibodies for the purification of IgE-BFs, the use of purified IgE-BFs, its individual optionally glycosylated proteins and/or fragments thereof for the prevention and/or treatment of allergy, and to pharmaceutical preparations containing them.
    Type: Grant
    Filed: June 4, 1986
    Date of Patent: August 7, 1990
    Assignee: Ciba-Geigy Corporation
    Inventor: Guy Delespesse
  • Patent number: 4945056
    Abstract: L60 is a novel monoclonal antibody that will react with Leu 22. It is equally reactive in formalin, ZnSO.sub.4 or Bouin's fixed, paraffin-embedded tissues as well as in frozen tissues.
    Type: Grant
    Filed: December 30, 1987
    Date of Patent: July 31, 1990
    Assignee: Becton, Dickinson and Company
    Inventors: David W. Buck, Jane M. Bindl
  • Patent number: 4942131
    Abstract: Disclosed are a monoclonal antibody which recognizes specifically an O-acylsialic acid containing sugar chain and a method for preparing a hybridoma capable of producing the monoclonal antibody, which comprises using an autoimmune disease animal as the immunization animal and/or using a substance containing said sugar chain adsorbed on Salmonella minesota bacteria as the immunogen.The monoclonal antibody of the present invention capable of recognizing the above described sugar chain which may be considered to be antigens related with human cancer are very useful in diagnosis of cancer.
    Type: Grant
    Filed: June 3, 1988
    Date of Patent: July 17, 1990
    Assignee: Konica Corporation
    Inventors: Masahiko Yamasaki, Kiyomi Sunaga, Yoshitaka Nagai
  • Patent number: 4933435
    Abstract: Immunoglobulins are purified by adsorption upon and adsorbent therefor using a buffer having a pH value of about pH 6 to pH 10 and containing at least one polycarboxylic acid in a concentration of about 0.5 M to about 0.9 M.
    Type: Grant
    Filed: April 5, 1989
    Date of Patent: June 12, 1990
    Assignee: Bioprobe International
    Inventor: That T. Ngo
  • Patent number: 4929543
    Abstract: The present invention provides a process for the determination of an antibody in human body fluids according to the immunoassay principle in which a sample containing the antibody to be determined is incubated with at least two different receptors R.sub.1 and R.sub.2, of which one receptor R.sub.1 carries an antigenic determinant specific for the antibody to be determined and one receptor R.sub.2 carries a label to form bound and unbound label, the part which contains the bound label is separated from the part which contains the unbound label and the label is measured in one of the two parts, wherein, for the control, instead of the sample, there is used a standard solution which contains a conjugate of a bindable non-human antibody or a Fab or F(ab').sub.2 fragment thereof which non-human component binds with a receptor R.sub.s which also binds to the antibody to be determined, and a human immunoglobulin or the Fc part thereof.
    Type: Grant
    Filed: May 12, 1988
    Date of Patent: May 29, 1990
    Assignee: Boehringer Mannheim GmbH
    Inventors: Rosemarie Kientsch-Engel, Walter Worner, Gerd Kleinhammer
  • Patent number: 4925800
    Abstract: Hybridomas producing antibodies having specific binding affinity against Pneumocystis carinii having been obtained and a method and kit for detecting P. carinii infection in humans having been described.
    Type: Grant
    Filed: December 4, 1986
    Date of Patent: May 15, 1990
    Assignee: The United States of America as represented by the Department of Health and Human Services
    Inventors: Joseph A. Kovacs, Henry Masur