Abstract: Monoclonal antibody to Treponema denticola ATCC 33520 and a continuous cell line for its production is disclosed. These antibodies are particularly useful in immunoassays for detecting the presence of these microorganisms in the oral cavity.

Abstract: The present invention relates to monoclonal antibodies having high affinity to human pancreatic PLA.sub.2, the production thereof, hybridomas producing them and an immunoassay for human pancreatic PLA.sub.2 using them.

Abstract: Picogram amounts of DNA can be detected in a sample by the use of high affinity single-stranded DNA binding proteins. The assay is applicable not only to pure DNA samples but also to samples containing significant amounts of protein.

Abstract: Murine monoclonal antibodies specific to unique antigenic determinants on mammalian terminal deoxynucleotidyl transferases (TdT). The monoclonal antibodies specifically bind to TdT in a wide variety of mammalian cells including human, mouse, rat, rabbit and bovine origin. The monoclonal antibodies are secreted by hybridoma cell lines derived from fusion of murine plasmacytoma cells with splenocytes from mice immunized with TdT from bovine thymus cells. The monoclonal antibodies can detect small numbers of TdT-positive cells from monitoring of TdT-positive leukemias and lymphomas in multple species, including human.

Abstract: Rabbit-mouse hybridomas are cultured immediately after fusion in a suspension medium supplemented with normal rabbit serum. This results in stable hybridomas and provides a source of intact rabbit monoclonal antibody immunoglobulin of defined specificity. A hybridoma which secretes intact rabbit antibody specific for Group A Streptococcus is exemplified. Diagnostic compositions and test kits containing rabbit monoclonal antibodies produced from such hybridomas are described.This application is a continuation of application Ser. No. 07/189,754, filed May 3, 1988, which in turn is a continuation-in-part of application Ser. No.: 046,439, filed May 4, 1987, now abandoned.

Abstract: Thermochemically induced luminescence is generated in a fluorescent labeled organic compound containing a covalently bonded fluorescent label which is a polycyclic aromatic radical having at least three linearly fused benzene rings and capable of being excited to a fluorescent electronic excited state by energy transfer from an energy donor molecule or radical having an electronic excited state, by a process comprising generating an energy donor radical or molecule by a thermochemical reaction in the presence of the fluorescent label. The energy donor radical may be generated by a chemical reaction in the presence of the fluorescent labeled organic compound or the fluorescent labeled compound itself may be additionally labeled with a radical capable of being excited to an electronic excited state by a thermochemical reaction, i.e., by a process initiated by adsorption of thermal energy.

Abstract: Oligonucleotide probes for diagnosing predisposition to rheumatoid arthritis, capable of specifically hybridizing with5'-TACGGGGTTGR.sub.1 GAGAGCTT-3'or3'-AR.sub.2 GCCCCAACR.sub.3 CR.sub.2 CR.sub.2 CGAA-5'wherein A is adenine, C is cytosine, G is guanine, T is thymine, R.sub.1 is GT or TG, R.sub.2 is T or uracil, and R.sub.3 is CA or AC. Or, capable of specifically hybridizing with5'-GGAGCAGAR.sub.2 GCGGGCCGCGG-3'or3'-CCR.sub.2 CGR.sub.2 CR.sub.2 R.sub.3 CGCCCGGCGCC-5'wherein R.sub.1 is A or G, R.sub.2 is T or uracil, and R.sub.3 is R.sub.2 or C.

Abstract: A monoclonal antibody specific to a sugar chain containing N-glycolylneuraminic acid and has an ability to bind at least to N-glycolyl GM.sub.2 ganglioside, and producing methods of a hybridomas which produce the monoclonal antibodies are disclosed. The antibodies are extremely useful for clarification of carcinogenesis mechanism, cancer diagnosis and therapy. The hybridomas are manufactured by using an animal with autoimmune disease.

Abstract: Picogram amounts of DNA can be detected in a sample by the use of high-affinity, single-stranded DNA binding proteins. The assay is applicable not only to pure DNA samples but also to samples containing significant amounts of protein.

Abstract: A monoclonal antibody is described, having a specific reactivity with a cancer-associated antigen selected from pancreas cancer-associated antigen, intestinum crassum cancer-associated antigen and hepatoma-associated antigen and has the following characteristics:(1) it exists in a blood serum of pancreas cancer, intestinum crassum cancer and hepatoma patients;(2) it comprises glycoproteins;(3) it has a molecular weight of about 700,000 to 1,500,000, as measured by gel-filtration and determined by comparison with that of a known protein;(4) it has a maximum absorption of 2800 nm when dissolved in 0.1 M acetic acid buffer solution (pH 4.5); and(5) it is released at least in a cultured supernatant medium of establihed cell line of COLO-201, TE-1, TE-2, TE-3, NRC-12, MKN-45 and KATO III. The monoclonal antibody is utilized as a reagent for detecting pancreas cancer, intestinum crassum cancer and hepatoma. A process for preparing the monoclonal antibody and a process for preparing the reagent are also disclosed.

Abstract: Preparation of novel polypeptide sequences spanning amino acid position 61 of the ras protein, said sequences being characterized as containing a leucine in that position instead of the glutamine found in normal ras protein, the utilization of such polypeptides to prepare immunogen compositions utilizing such polypeptide covalently linked to immunogenic carrier materials, the production of antibodies elicited by such polypeptides, the screening of such antibodies to provide monoclonals specific to the P21Tleu61 transforming protein and to immunoassay and use in immunochemical methods employing such antibodies to determine the presence of the p21TLeu61 transforming protein.

Abstract: Antibodies specific for pancreatic alpha-amylase and methods for their production are described. These antibodies may be used for the detection and quantification of pancreatic alpha-amylase in biological fluids, tissue extracts, or extracts of stains of biological fluids.

Abstract: An assay procedure that is particularly valuable for detecting and/or determining the presence of threshold levels of analyte ligands in biological fluids. In one particularized and specialized aspect the disclosure is directed to procedures for detecting and/or determining threshold levels of hormone metabolites such as pregnanediol-3-glucuronide (P.sub.3 G) and estrone-3-glucuronide (E.sub.1 3G) in human urine. The assay consists of contacting a sample containing the analyte with a known amount of an antibody thereto and with a calibrated amount of the analyte itself that is conjugated to said solid support. When the level of the analyte in the sample exceeds a threshold level, such as 5 ug/ml for P.sub.3 G, the antibody will be insufficient to block all of the corresponding analyte on the solid support.

Abstract: Mouse-human hybridoma, which produces monoclonal antivirus-human antibody reactive to herpes simplex virus, varicella zoster virus, and cytomegalo virus, obtained by allowing a human antibody cell, which is sensitized to virus or protein or glycoprotein arising from virus, to fuse with a mouse myeloma cell in the presence of mitogen in vitro.

Abstract: The invention relates to novel purified human immunoglobulin E binding factors (IgE-BFs), its individual optionally glycosylated proteins, and fragments thereof, processes for the purification of IgE-BFs, novel monoclonal antibodies to lymphocyte cellular receptors for IgE (Fc.sub..epsilon. R) crossreacting with IgE-Fs, derivatives thereof, processes for the preparation of these antibodies and their derivatives, hybridoma cell lines that produce these antibodies, processes for the preparation of said hybridoma cell lines, the use of the monoclonal antibodies and their derivatives for the qualitative and quantitative determination of IgE-BFs, test kits containing the monoclonal antibodies and/or their derivatives, the use of the monoclonal antibodies for the purification of IgE-BFs, the use of purified IgE-BFs, its individual optionally glycosylated proteins and/or fragments thereof for the prevention and/or treatment of allergy, and to pharmaceutical preparations containing them.

Abstract: L60 is a novel monoclonal antibody that will react with Leu 22. It is equally reactive in formalin, ZnSO.sub.4 or Bouin's fixed, paraffin-embedded tissues as well as in frozen tissues.

Abstract: Disclosed are a monoclonal antibody which recognizes specifically an O-acylsialic acid containing sugar chain and a method for preparing a hybridoma capable of producing the monoclonal antibody, which comprises using an autoimmune disease animal as the immunization animal and/or using a substance containing said sugar chain adsorbed on Salmonella minesota bacteria as the immunogen.The monoclonal antibody of the present invention capable of recognizing the above described sugar chain which may be considered to be antigens related with human cancer are very useful in diagnosis of cancer.

Abstract: Immunoglobulins are purified by adsorption upon and adsorbent therefor using a buffer having a pH value of about pH 6 to pH 10 and containing at least one polycarboxylic acid in a concentration of about 0.5 M to about 0.9 M.

Abstract: The present invention provides a process for the determination of an antibody in human body fluids according to the immunoassay principle in which a sample containing the antibody to be determined is incubated with at least two different receptors R.sub.1 and R.sub.2, of which one receptor R.sub.1 carries an antigenic determinant specific for the antibody to be determined and one receptor R.sub.2 carries a label to form bound and unbound label, the part which contains the bound label is separated from the part which contains the unbound label and the label is measured in one of the two parts, wherein, for the control, instead of the sample, there is used a standard solution which contains a conjugate of a bindable non-human antibody or a Fab or F(ab').sub.2 fragment thereof which non-human component binds with a receptor R.sub.s which also binds to the antibody to be determined, and a human immunoglobulin or the Fc part thereof.

Abstract: Hybridomas producing antibodies having specific binding affinity against Pneumocystis carinii having been obtained and a method and kit for detecting P. carinii infection in humans having been described.